Publikasi Scopus FKUI 2021 per tanggal 31 Agustus 2021 (582 artikel)

Pujianto D., Oktarina M., Sharma Sharaswati I., Yulhasri Y.
8745734300;57225172840;57225177505;57225180520;
Hydrogen peroxide has adverse effects on human sperm quality parameters, induces apoptosis, and reduces survival
2021
Journal of Human Reproductive Sciences
14
2
121
128
Department of Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Master Program for Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Pujianto, D., Department of Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Oktarina, M., Master Program for Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Sharma Sharaswati, I., Master Program for Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Yulhasri, Y., Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Background: One of the causes of male fertility disorders is the exposure of oxidative stress on the human sperm. Understanding the mechanism of disturbance is important to develop a better treatment for infertile or subfertile patients. Aims: The aim of this study was to analyze the effects of hydrogen peroxide (H2O2) on human sperm quality parameters and cell survival. Settings and Design: This study used an experimental design. Materials and Methods: Sperm cells from 15 donors were washed in a Percoll gradient and dissolved in Biggers, Whitter, and Whittingham medium. Cells were incubated with H2O2at various concentrations from 0 to 250 μM for 2 h. Sperm viability was examined by eosin assay, sperm kinetic by computer-assisted sperm analyzer, sperm penetration by cervical mucus penetration assay, and membrane integrity by hypo-osmotic swelling test. Sperm capacitation, apoptosis, and cell survival were analyzed using western immunoblotting. Statistical Analysis Used: One-way ANOVA on SPSS 21 combined with post hoc LSD test was used to analyze differences among the groups. A P < 0.05 was considered significant. Results: Sperm viability and kinetic were significantly reduced at H2O2concentrations of 200 and 250 μM. H2O2reduced sperm capability to penetrate cervical mucus and also damage cell membrane integrity at all concentrations used. H2O2significantly inhibited sperm capacitation, indicated by reduced total tyrosine phosphorylation. H2O2exposure stimulated activation of caspase 3 and significantly reduced phosphorylated AKT at all concentrations used. Conclusions: H2O2comprehensively inhibits sperm qualities related to the capacity to fertilize oocyte, stimulates caspase activity, and inhibits cell survival. © 2021 Wolters Kluwer Medknow Publications. All rights reserved.
AKT; capacitation; caspase; hydrogen peroxide; sperm
Wolters Kluwer Medknow Publications
9741208
Article
Q3
484
10359