Publikasi Scopus FKUI 2021 per tanggal 31 Agustus 2021 (582 artikel)

Smith O.J., Wicaksana A., Davidson D., Spratt D., Mosahebi A.
54881691700;57211445559;55553306500;7004394482;55985666400;
An evaluation of the bacteriostatic effect of platelet-rich plasma
2021
International Wound Journal
18
4
448
456
Department of Plastic Surgery, Royal Free Hospital, London, United Kingdom; Division of Surgery and Interventional Science, University College London, London, United Kingdom; Division of Plastic Surgery, Faculty of Medicine Universitas Indonesia/Dr. Cipto Mangunkusumo National Hospital, Jakarta, Indonesia; Microbial Diseases, Eastman Dental Institute, Faculty of Medical Sciences, University College London, London, United Kingdom
Smith, O.J., Department of Plastic Surgery, Royal Free Hospital, London, United Kingdom, Division of Surgery and Interventional Science, University College London, London, United Kingdom; Wicaksana, A., Division of Surgery and Interventional Science, University College London, London, United Kingdom, Division of Plastic Surgery, Faculty of Medicine Universitas Indonesia/Dr. Cipto Mangunkusumo National Hospital, Jakarta, Indonesia; Davidson, D., Microbial Diseases, Eastman Dental Institute, Faculty of Medical Sciences, University College London, London, United Kingdom; Spratt, D., Microbial Diseases, Eastman Dental Institute, Faculty of Medical Sciences, University College London, London, United Kingdom; Mosahebi, A., Department of Plastic Surgery, Royal Free Hospital, London, United Kingdom, Division of Surgery and Interventional Science, University College London, London, United Kingdom
Chronic wounds are a considerable health burden with high morbidity and poor rates of healing. Colonisation of chronic wounds by bacteria can be a significant factor in their poor healing rate. These bacteria can develop antibiotic resistance over time and can lead to wound infections, systemic illness, and occasionally amputation. When a large number of micro-organisms colonise wounds, they can lead to biofilm formation, which are self-perpetuating colonies of bacteria closed within an extracellular matrix, which are poorly penetrated by antibiotics. Platelet-rich plasma (PRP) is an autologous blood product rich in growth factors and cytokines that are involved in an inflammatory response. PRP can be injected or applied to a wound as a topical gel, and there is some interest regarding its antimicrobial properties and whether this can improve wound healing. This study aimed to evaluate the in vitro bacteriostatic effect of PRP. PRP was collected from healthy volunteers and processed into two preparations: activated PRP—activated with calcium chloride and ethanol; inactivated PRP. The activity of each preparation against Staphylococcus aureus and Staphylococcus epidermis was evaluated against a control by three experiments: bacterial kill assay to assess planktonic bacterial growth; plate colony assay to assess bacterial colony growth; and colony biofilm assay to assess biofilm growth. Compared with control, both preparations of PRP significantly inhibited growth of planktonic S aureus and S epidermis. Activated PRP reduced planktonic bacterial concentration more than inactivated PRP in both bacteria. Both PRP preparations significantly reduced bacterial colony counts for both bacteria when compared with control; however, there was no difference between the two. There was no difference found between biofilm growth in either PRP against control or against the other preparation. This study demonstrates that PRP does have an inhibitory effect on the growth of common wound pathogens. Activation may be an important factor in increasing the antimicrobial effect of PRP. However, we did not find evidence of an effect against more complex bacterial colonies. © 2021 The Authors. International Wound Journal published by Medicalhelplines.com Inc (3M) and John Wiley & Sons Ltd.
antimicrobial therapy; chronic wounds; platelet-rich plasma
alcohol; calcium chloride; Article; bacterial count; bacterial growth; bacterial strain; bacteriostatic activity; bacterium colony; biofilm; controlled study; extracellular matrix; in vitro study; nonhuman; Staphylococcus aureus; Staphylococcus epidermidis; thrombocyte activation; thrombocyte rich plasma
John Wiley and Sons Inc
17424801
33476481
Article
Q1
867
5385