Publikasi Scopus 926 artikel (Per 14 Maret 2022)

Umiatin, Indrasari W., Taryudi, Sari P., Mazfufah N.F., Rosadi I.
57202292099;56069603500;57003576500;55776482300;57215595521;57204720098;
Effect of pulse electromagnetic field exposure on the expression of lipo protein lipase (LPL) on the differentiation of mesenchymal stem cell
2021
Journal of Physics: Conference Series
2019
1
012107
Department of Physics, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Indonesia; Department of Electronic Engineering, Faculty of Engineering, Universitas Negeri Jakarta, Indonesia; Department of Biology, Faculty of Medicine, Universitas Indonesia, Indonesia, Jl. Rawamangun Muka, Jakarta, 13220, Indonesia; Stem Cells and Tissue Engineering Research Center, IMERI, Faculty of Medicine, Universitas Indonesia, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Samarinda, Indonesia
Umiatin, Department of Physics, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Indonesia; Indrasari, W., Department of Physics, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Indonesia; Taryudi, Department of Electronic Engineering, Faculty of Engineering, Universitas Negeri Jakarta, Indonesia; Sari, P., Department of Biology, Faculty of Medicine, Universitas Indonesia, Indonesia, Jl. Rawamangun Muka, Jakarta, 13220, Indonesia; Mazfufah, N.F., Stem Cells and Tissue Engineering Research Center, IMERI, Faculty of Medicine, Universitas Indonesia, Indonesia; Rosadi, I., Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Samarinda, Indonesia
Pulsed electromagnetic fields (PEMFs) have an important role in cell differentiation. Previous study reported that PEMFs had positive and negative effect towards cell differentiation that depends on their frequencies applied to the cells. Human adipose-derived stem cells (ASCs) are mesenchymal stem cells that have an ability to differentiate into several types of cell including adipocytes, chondrocytes and osteocytes. This study aimed to evaluate the effect of human ASCs towards their adipogenic differentiation during PEMFs exposure. Human ASCs were isolated from adipose tissue. The cells then cultured in specific medium of adipocyte that induced ASCs differentiation along with PEMFs exposure. The maximum magnetic field used is 2 mT with a frequency of 75 Hz. To confirm the effect of PEMFs exposure towards adipogenic differentiation, mRNA expression of lipo protein lipase (LPL) was measured in mRNA expression level. The results showed that ASCs cultured on adipogenic differentiation without PEMFs exposure gradually increased LPL expression until day 14 of observation, while ASCs with PEMFs exposure significantly decreased LPL expression from day 2 to day 14. Based on the results, we concluded that PEMFs exposure can inhibit LPL expression that suppressed adipogenic differentiation. © 2021 Institute of Physics Publishing. All rights reserved.
Electromagnetic field effects; Electromagnetic fields; Proteins; Stem cells; Adipogenic differentiations; Adipose derived stem cells; Cell differentiation; Cell-be; Cell/B.E; Electromagnetic field exposure; Human adipose; Lipo proteins; Mesenchymal stem cell; Pulsed electromagnetic fields; Cell culture
IOP Publishing Ltd
17426588
Conference Paper
Q4
210
18731