Publikasi Scopus 926 artikel (Per 14 Maret 2022)

Gustiananda M., Sulistyo B.P., Agustriawan D., Andarini S.
6507570171;57215020738;55382929300;8716259500;
Immunoinformatics analysis of sars-cov-2 orf1ab polyproteins to identify promiscuous and highly conserved t-cell epitopes to formulate vaccine for indonesia and the world population
2021
Vaccines
9
12
1459
1
Department of Biomedicine, School of Life Sciences, Indonesia International Institute for Life Sciences, Jl. Pulomas Barat Kav 88, Jakarta, 13210, Indonesia; Department of Bioinformatics, School of Life Sciences, Indonesia International Institute for Life Sciences, Jl. Pulomas Barat Kav 88, Jakarta, 13210, Indonesia; Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, University of Indonesia, Persahabatan Hospital, Jl Persahabatan Raya 1, Jakarta, 13230, Indonesia
Gustiananda, M., Department of Biomedicine, School of Life Sciences, Indonesia International Institute for Life Sciences, Jl. Pulomas Barat Kav 88, Jakarta, 13210, Indonesia; Sulistyo, B.P., Department of Biomedicine, School of Life Sciences, Indonesia International Institute for Life Sciences, Jl. Pulomas Barat Kav 88, Jakarta, 13210, Indonesia; Agustriawan, D., Department of Bioinformatics, School of Life Sciences, Indonesia International Institute for Life Sciences, Jl. Pulomas Barat Kav 88, Jakarta, 13210, Indonesia; Andarini, S., Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, University of Indonesia, Persahabatan Hospital, Jl Persahabatan Raya 1, Jakarta, 13230, Indonesia
SARS-CoV-2 and its variants caused the COVID-19 pandemic. Vaccines that target conserved regions of SARS-CoV-2 and stimulate protective T-cell responses are important for reducing symptoms and limiting the infection. Seven cytotoxic (CTL) and five helper T-cells (HTL) epitopes from ORF1ab were identified using NetCTLpan and NetMHCIIpan algorithms, respectively. These epitopes were generated from ORF1ab regions that are evolutionary stable as reflected by zero Shannon’s entropy and are presented by 56 human leukocyte antigen (HLA) Class I and 22 HLA Class II, ensuring good coverage for the Indonesian and world population. Having fulfilled other criteria such as immunogenicity, IFNγ inducing ability, and non-homology to human and microbiome peptides, the epitopes were assembled into a vaccine construct (VC) together with β-defensin as adjuvant and appropriate linkers. The VC was shown to have good physicochemical characteristics and capability of inducing CTL as well as HTL responses, which stem from the engagement of the vaccine with toll-like receptor 4 (TLR4) as revealed by docking simulations. The most promiscuous peptide899WSMATYYLF907 was shown via docking simulation to interact well with HLA-A*24:07, the most predominant allele in Indonesia. The data presented here will contribute to the in vitro study of T-cell epitope mapping and vaccine design in Indonesia. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Cytotoxic T-cells; Helper T-cells; HLA-A*24:07; Human leukocyte antigen; Immunoinformatics; Multi-epitope peptide-based vaccine; SARS-CoV-2; T-cell epitopes
epitope; gamma interferon; HLA A antigen; HLA antibody; T lymphocyte receptor; toll like receptor 4; allele; allergenicity; amino acid sequence; antigenicity; Article; binding affinity; CD8+ T lymphocyte; controlled study; cytotoxic T lymphocyte; endoplasmic reticulum; entropy; epitope mapping; gene frequency; gene structure; HLA typing; human; human cell; hydrophilicity; immune response; immunogenicity; immunoinformatics; Indonesia; microbiome; molecular docking; open reading frame; peptide synthesis; protein interaction; protein secondary structure; protein structure; sequence alignment; sequence analysis; sequence homology; Severe acute respiratory syndrome coronavirus 2; vaccination
MDPI
2076393X
Article
Q1
1296
2913