Publikasi Scopus 926 artikel (Per 14 Maret 2022)

Zahrah A., Muharam R., Luky Satria Marwali M., Ocktariyana, Deraya I.E., Asmarinah
57205418186;57191492732;57225892448;57210235127;57216313121;15820317600;
MRNA expression and DNA methylation level of the MMP-2 gene in peritoneal endometriosis
2021
Journal of the Pakistan Medical Association
71
2
S112
S115
Master Program of Biomedical Science, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Department of Obstetric and Ginecology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Department of Obstetric and Ginecology, Fatmawati Central General Hospital, Jakarta, Indonesia; Department Miwifery, Politeknik Kesehatan Palembang, Ministry of Health, Indonesia; Department of Medical Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
Zahrah, A., Master Program of Biomedical Science, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Muharam, R., Department of Obstetric and Ginecology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Luky Satria Marwali, M., Department of Obstetric and Ginecology, Fatmawati Central General Hospital, Jakarta, Indonesia; Ocktariyana, Department Miwifery, Politeknik Kesehatan Palembang, Ministry of Health, Indonesia; Deraya, I.E., Department of Medical Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Asmarinah, Master Program of Biomedical Science, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
Objective: Endometriosis is a multifactorial disease that affects 10% of women of childbearing age. Its development is known to be related to the MMP-2 gene. Specifically, the expression of MMP-2 is increase in endometriosis. The aim of this study was to evaluate the correlation between mRNA expression and DNA methylation levels of the MMP-2 gene in peritoneal endometriosis. Methods: This study used a cross-sectional design. The samples included peritoneal endometriosis tissue from women with endometriosis and normal endometrial tissue from women without endometriosis. Twenty samples of each type were taken, and the women were 20-45 years of age. Peritoneal endometriosis tissue was acquired using the laparoscopic technique, while normal endometrial tissue was taken with the microcuretase technique. The mRNA expression of the MMP-2 gene was analysed with qRT-PCR, and the level of DNA methylation of the MMP-2 gene was analysed with a methylation-specific PCR method. Results: The mRNA expression of MMP-2 gene in peritoneal endometriosis tissue was increased, and there were significant differences between peritoneal endometriosis tissue and normal endometrial tissue. The MMP-2 gene was hypermethylated, but there was no significant difference (p = 0.596) between peritoneal endometriosis tissue and endometrial tissue in terms of methylation. This study did not show a significant correlation between mRNA expression and DNA methylation levels of the MMP-2 gene (p = 0.769, r = 0.070). Conclusion: The increase in MMP-2 gene expression in peritoneal endometriosis tissue is likely not only due to hypermethylation; there are other factors that might play a role. © 2021 Pakistan Medical Association. All rights reserved.
cytoskeleton. (JPMA 71: S-112 [Suppl. 2]; 2021); DNA methylation; endometriosis; MMP-2
gelatinase A; messenger RNA; cross-sectional study; DNA methylation; endometriosis; endometrium; female; genetics; human; metabolism; Cross-Sectional Studies; DNA Methylation; Endometriosis; Endometrium; Female; Humans; Matrix Metalloproteinase 2; RNA, Messenger
Pakistan Medical Association
00309982
33785954
Conference Paper
Q4
242
17081