Publikasi Scopus 926 artikel (Per 14 Maret 2022)

633

Authors

Nadia Y.F., Syahrani R.A., Arumsari S., Sadikin M., Wanandi S.I.

Author S Id

57428347800;57204103147;57193202336;36981411100;36099320700;

Title

Effect of Cell Culture Medium on the Proliferation and Stemness of CD24-/CD44+ Human Breast Cancer Stem Cells

Year

2021

Source Title

Indonesian Biomedical Journal

Volume

Issue

Art No

Page Start

355

Page End

363

Page Count

Cited By

Link

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123550895&doi=10.18585%2fINABJ.V13I4.1674&partnerID=40&md5=f0c363bff3a069d364bc71970a109469

Affiliations

Master’s Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia; Molecular Biology and Proteomics Core Facilities, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia

Authors With Affiliations

Nadia, Y.F., Master’s Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia; Syahrani, R.A., Molecular Biology and Proteomics Core Facilities, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia; Arumsari, S., Molecular Biology and Proteomics Core Facilities, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia; Sadikin, M., Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia; Wanandi, S.I., Molecular Biology and Proteomics Core Facilities, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia

Abstract

BACKGROUND: Cancer stem cells (CSCs) is defined as tumor initiating cells within tumor that maintain stemness properties and tumorigenicity. Extracellular pH of CSCs in in vitro condition is important for supporting cell proliferation which may also regulate the expression of stemness markers such as OCT4. This work aimed to examine the effect of cell culture media on the proliferation and stemness of human breast cancer stem cells (BCSCs). RESULTS: Our results showed a higher viable cell number and proliferation of BCSCs in DMEM/F-12 HEPES (-) compared to HEPES (+) medium until 4 day incubation. OCT4 mRNA and protein level, as well as MFU of BCSCs were significantly higher in HEPES (-) compared to HEPES (+) medium on day 2. CONCLUSION: DMEM/F-12 medium without HEPES facilitates CD24-/CD44+ BCSCs to have higher proliferation and stemness on day 2 incubation compared to those with HEPES. © 2021. All Rights Reserved.

Author Keywords

breast cancer; cancer stem cell; OCT4; proliferation; stemness

Index Keywords

Publisher

Prodia Education and Research Institute

Issn

20853297

Isbn

Pubmed Id

Document Type

Article

Sjr Best Quartile

Sjr

160

Sjr Rank

22228