Publikasi Scopus 926 artikel (Per 14 Maret 2022)

Jusman S.W.A., Azzizah I.N., Sadikin M., Hardiany N.S.
36518792100;57223677370;57205093115;57192910605;
Is the mitochondrial function of keloid fibroblasts affected by cytoglobin?
2021
Malaysian Journal of Medical Sciences
28
2
39
47
Department of Biochemistry & Molecular Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Faculty of Medicine Universitas Indonesia, Indonesia; Center of Hypoxia & Oxidative Stress Studies, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
Jusman, S.W.A., Department of Biochemistry & Molecular Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia, Center of Hypoxia & Oxidative Stress Studies, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Azzizah, I.N., Faculty of Medicine Universitas Indonesia, Indonesia; Sadikin, M., Department of Biochemistry & Molecular Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia, Center of Hypoxia & Oxidative Stress Studies, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Hardiany, N.S., Department of Biochemistry & Molecular Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia, Center of Hypoxia & Oxidative Stress Studies, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
Background: A keloid is a benign skin tumour characterised by excessive proliferation of fibroblasts, a process that requires a sufficient amount of energy. The energy needs are associated with adequate oxygen (O2) flow and well-functioning mitochondria. It is known that cytoglobin (CYGB) has a function in O2 distribution. The aim of the present study was to explore whether the inhibition of CYGB expression caused impaired mitochondrial function of keloid fibroblasts. Methods: An in vitro study was conducted on a keloid fibroblast derived from our previous study. The study was carried out in the laboratory of the Biochemistry & Molecular Biology Department, Faculty of Medicine, Universitas Indonesia (FMUI), from July to December 2018. CYGB expression was inhibited by small interfering ribonucleic acid (siRNA) and CYGB. Analysis of mitochondrial function was observed through peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), a mitochondrial biogenesis marker and the activity of the succinate dehydrogenase (SDH) enzyme in mitochondria. Results: The CYGB gene and protein were downregulated after treatment with CYGB siRNA. Inhibition of CYGB expression with siRNA also tended to decrease the levels of PGC-1α messenger ribonucleic acid (mRNA) and protein, as well as SDH enzyme activity. Conclusion: Inhibition of CYGB expression with siRNA tended to decrease mitochondrial biogenesis and function. This may be useful for understanding the excessive proliferation of fibroblasts in keloids and for development of treatment for keloids. © Penerbit Universiti Sains Malaysia, 2021.
CYGB siRNA; Keloid fibroblasts; PGC-1α; SDH enzyme activity
cytoglobin; oxygen; peroxisome proliferator activated receptor gamma coactivator 1alpha; small interfering RNA; succinate dehydrogenase; Article; cell function; cell proliferation; colorimetry; controlled study; down regulation; enzyme activity; enzyme linked immunosorbent assay; fibroblast; gene expression; genetic transfection; human; human cell; in vitro study; keloid; mitochondrial biogenesis; mitochondrial genetics; mRNA expression level; protein expression; protein isolation; real time polymerase chain reaction; reverse transcription; RNA isolation
Penerbit Universiti Sains Malaysia
1394195X
Article
Q3
394
12287