Publikasi Scopus FKUI Tahun 2010 s/d 2020 (data Per 3 Februari 2021)

Tarini N.M.A., Wahid M.H., Ibrahim F., Yasmon A., Djauzi S.
56669724600;57192887722;54886001500;41462004500;23495847800;
Development of multiplex-PCR assay for rapid detection of Candida spp.
2010
Medical Journal of Indonesia
19
2
83
87
6
Department of Clinical Microbiology, Medical Faculty, University of Udayana, Bali, Indonesia; Department of Microbiology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Allergic-Immunology Division, Department of Internal Medicine, Faculty of Medicine, University of Indonesia–Ciptomangunkusumo Hospital, Jakarta, Indonesia
Tarini, N.M.A., Department of Clinical Microbiology, Medical Faculty, University of Udayana, Bali, Indonesia; Wahid, M.H., Department of Microbiology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Ibrahim, F., Department of Microbiology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Yasmon, A., Department of Microbiology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Djauzi, S., Allergic-Immunology Division, Department of Internal Medicine, Faculty of Medicine, University of Indonesia–Ciptomangunkusumo Hospital, Jakarta, Indonesia
Aim Candida spp. infection commonly occur in immunocompromised patients. Biochemical assay for identification of Candida spp. is time-consuming and shows many undetermined results. Specific detection for antibody, antigen and metabolites of Candida spp. had low sensitivity and specificity. In this study, we developed a rapid diagnostic method, Multiplex-PCR, to identify Candida spp. Methods Five Candida spp. isolates were cultured, identified with germ tube and API® 20 C AUX (BioMerieux® SA) kit. Furthermore, DNA was purified by QIAamp DNA mini (Qiagen®) kit for Multiplex-PCR assay. Results DNA detection limit by Multiplex-PCR assays for C. albicans, C. tropicalis, C. parapsilosis, C. krusei and C. glabrata were 4 pg, 0,98 pg, 0,98 pg, 0,5 pg and 16 pg respectively. This assay was also more sensitive than culture in that Multiplex-PCR could detect 2.6-2.9 x 100 CFU/ml, whereas culture 2.6-2.9 x 102 CFU/ml. Conclusion Multiplex-PCR is much more sensitive than culture and thus, can be recommended as a sens tive and specific assay for identification of Candida spp. © 2010, Faculty of Medicine, Universitas Indonesia. All rights reserved.
Candida spp.; Multiplex-PCR
Candida; fungal cell culture; human; human cell; limit of detection; multiplex polymerase chain reaction; nonhuman
Faculty of Medicine, Universitas Indonesia
08531773
Article
Q4