Publikasi Scopus FKUI Tahun 2010 s/d 2020 (data Per 3 Februari 2021)

Pawitan J.A., Damayanti L., Arleni, Swantari N.M.
6508348067;6506640886;57192888839;57192911834;
Cytology technique: Development of a simple spot method for cultured cell suspension
2010
Medical Journal of Indonesia
19
1
26
31
1
Department of Histology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Depatment of Biology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Immunology and Endocrinology Integrated Laboratory, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Division of Plastic Surgery, Department of Surgery, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
Pawitan, J.A., Department of Histology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Damayanti, L., Department of Histology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Arleni, Depatment of Biology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia, Immunology and Endocrinology Integrated Laboratory, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Swantari, N.M., Division of Plastic Surgery, Department of Surgery, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
Aim To develop a simple spot method to attach cultured cells in suspension on to a glass slide. Methods We compared three approaches using both conventional and special glass slide (Shandon-Polysin)., either without additional fetal bovine serum (FBS), or with addition of 3 or 10 μl of FBS to a 20 μl sample (altogether there were six approaches). The slides were examined qualitatively for the background color, boundary color and intactness, and whether there were folded and detached parts. Further, for each slide, the attached intact cells were counted, and the percentage of attached intact cells per number of spotted cells was calculated. The difference in attach intact cells between different approaches was analyzed by ANOVA using SPSS 13.0 for windows. Results There were no significant difference in the percentage of attached intact cells between the six approaches (P= 0.804), though the approach using special glass slide without additional FBS (FBS final concentration 5%) yield the highest percentage of attached intact cells, showed clean background without folded parts. Conclusions We have developed a simple spot method for cultured cell suspension, and the best approach to make spot specimen is using special glass slide with 5% FBS in the cell suspension. © 2010, Faculty of Medicine, Universitas Indonesia. All rights reserved.
Fetal bovine serum; Special glass slide; Spot specimen
Article; cell counting; cell culture technique; cell suspension; cytology; micropipette; microscope slide; microscopy; simple spot method; staining
Faculty of Medicine, Universitas Indonesia
08531773
Article
Q4