Publikasi Scopus FKUI Tahun 2010 s/d 2020 (data Per 3 Februari 2021)

Hardiany N.S., Sadikin M., Wanandi S.I.
57192910605;36981411100;36099320700;
Gene expression of manganese superoxide dismutase in human glioma cells
2010
Medical Journal of Indonesia
19
1
21
25
4
Departement of Biochemistry and Molecular Biology, Faculty of Medicine University of Indonesia, Jakarta, Indonesia; Faculty of Medicine University of Indonesia, Jakarta, Indonesia
Hardiany, N.S., Departement of Biochemistry and Molecular Biology, Faculty of Medicine University of Indonesia, Jakarta, Indonesia, Faculty of Medicine University of Indonesia, Jakarta, Indonesia; Sadikin, M., Departement of Biochemistry and Molecular Biology, Faculty of Medicine University of Indonesia, Jakarta, Indonesia; Wanandi, S.I., Departement of Biochemistry and Molecular Biology, Faculty of Medicine University of Indonesia, Jakarta, Indonesia
Aim This study analyze the MnSOD gene expression as endogenous antioxidant in human glioma cells compared with leucocyte cells as control. Methods MnSOD gene expression of 20 glioma patients was analyzed by measuring the relative expression of mRNA and enzyme activity of MnSOD in brain and leucocyte cells. The relative expression of mRNA MnSOD was determined by using quantitative Real Time RT-PCR and the enzyme activity of MnSOD using biochemical kit assay (xantine oxidase inhibition). Statistic analysis for mRNA and enzyme activity of MnSOD was performed using Kruskal Wallis test. Results mRNA of MnSOD in glioma cells of 70% sample was 0.015–0.627 lower, 10% was 1.002-1.059 and 20% was 1.409-6.915 higher than in leucocyte cells. Also the specific activity of MnSOD enzyme in glioma cells of 80% sample showed 0,064-0,506 lower and 20% sample was 1.249-2.718 higher than in leucocyte cells. Conclusion MnSOD gene expression in human glioma cells are significantly lower than its expression in leucocytes cells. © 2010, Faculty of Medicine, Universitas Indonesia. All rights reserved.
Gene expression; Glioma; MnSOD
manganese superoxide dismutase; xanthine oxidase; agar gel electrophoresis; Article; biochemical analysis; clinical article; controlled study; cross-sectional study; enzyme activity; enzyme specificity; gene amplification; gene expression; glioma cell; human; human cell; leukocyte; real time polymerase chain reaction; reverse transcription polymerase chain reaction; RNA isolation
Faculty of Medicine, Universitas Indonesia
08531773
Article
Q4