Publikasi Scopus FKUI Tahun 2010 s/d 2020 (data Per 3 Februari 2021)

Firdaus M., Astawan M., Muchtadi D., Wresdiyati T., Waspadji S., Karyono S.S.
55471418600;55622754000;54911954100;6507796731;8678136400;57192907536;
Prevention of endothelial dysfunction in streptozotocin-induced diabetic rats by Sargassum echinocarpum extract
2010
Medical Journal of Indonesia
19
1
32
35
9
Laboratory of Biochemistry, Faculty of Fisheries and Marine Science University of Brawijaya, Malang, Indonesia; Department of Food Science, Faculty of Agriculture Technology Bogor Agriculture University, Bogor, Indonesia; Laboratory of Anatomy, Faculty of Veterinary Bogor Agriculture University, Bogor, Indonesia; Internal Medicine Department, Faculty of Medicine University of Indonesia, Jakarta, Indonesia; Laboratory of Pharmacology, Faculty of Medicine University of Brawijaya, Malang, Indonesia
Firdaus, M., Laboratory of Biochemistry, Faculty of Fisheries and Marine Science University of Brawijaya, Malang, Indonesia; Astawan, M., Department of Food Science, Faculty of Agriculture Technology Bogor Agriculture University, Bogor, Indonesia; Muchtadi, D., Department of Food Science, Faculty of Agriculture Technology Bogor Agriculture University, Bogor, Indonesia; Wresdiyati, T., Laboratory of Anatomy, Faculty of Veterinary Bogor Agriculture University, Bogor, Indonesia; Waspadji, S., Internal Medicine Department, Faculty of Medicine University of Indonesia, Jakarta, Indonesia; Karyono, S.S., Laboratory of Pharmacology, Faculty of Medicine University of Brawijaya, Malang, Indonesia
Aim This study aimed to elicit the protective effect of Sargassum echinocarpum extract on endothelial dysfunction in thoracic aorta of streptozotocin-induced diabetic rats. Methods The animals were divided into 5 groups. The first was normal, the second was diabetic non treated animals. The third to fifth groups were the diabetic animals which given Sargassum echinocarpum extract (150; 300, and 450 mg kg-1 body weight, respectively) by oral gavage and extract treatment was given for 12 weeks. Diabetes was induced by single administration of streptozotocin (45 mg kg-1, i.p.), dissolved in freshly prepared 0.1 M citrate buffer, pH 4.5. Diabetes was confirmed ten days latter in streptozotocin induced animals showing blood glucose levels > 200 mg dL-1 (11.1 mmol L-1) as monitored in the blood from tail vein using glucometer. After the treatment period, the blood serum acquired was used for antioxidant enzymes assays and the thoracic aorta was used for vasorelaxation assay. Results There was a significant decrease in the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-px) in diabetic rats (3.31 ± 0.12;67.17 ± 0.62;35.10 ± 0.83) comaped to control rats (9.97 ± 0.12;185.31 ± 0.23;116.38 ± 0.88). Administration of Sargassum extract increased the activity of SOD, CAT, and GSH-px. The diabetic rats exhibit endothelial dysfunction as shown by loss of vasodilatory response to acethylcholine (ACH). This was restored by administration of Sargassum extract. Conclusion Sargassum echinocarpum extract ameliorates oxidative stress and reverses the endothelial dysfunction associated with diabetes. This effect appears to be due to its antioxidant properties. © 2010, Faculty of Medicine, Universitas Indonesia. All rights reserved.
Endothelium dependent relaxation; Oxidative stress; Sargassum echinocarpum; Thoracic aorta
acetylcholine; algal extract; catalase; glutathione peroxidase; Sargassum echinocarpum extract; superoxide dismutase; unclassified drug; animal model; animal tissue; antioxidant assay; Article; concentration response; controlled study; drug mechanism; endothelial dysfunction; glucose blood level; male; muscle isometric contraction; nonhuman; rat; Sargassum; Sargassum echinocarpum; streptozotocin-induced diabetes mellitus; thoracic aorta; vasodilatation
Faculty of Medicine, Universitas Indonesia
08531773
Article
Q4