Publikasi Scopus FKUI Tahun 2010 s/d 2020 (data Per 3 Februari 2021)

Sardjono C.T., Setiawan M., Suyatna F.D., Japutri I., Setiawan B., Sandra F.
57204406495;57202415005;56039633100;57192906835;57220394955;56016164200;
Immunogenicity characterization of mononucleated cells originated from umbillical cord blood
2010
Medical Journal of Indonesia
19
1
14
20
2
Stem Cell and Cancer Institute, Jl. A. Yani no 2. Pulo Mas, Jakarta, 13210, Indonesia; Microbiology Department, Faculty of Medicine, Maranatha Christian University, Bandung, 40164, Indonesia; Department of Pharmacology and Therapeutics, Medical Faculty, University of Indonesia, Jakarta, Indonesia; Kalbe Farma Tbk. Jl. Let. Jend. Suprapto Kav 4. Cempaka Putih, Jakarta, 10510, Indonesia
Sardjono, C.T., Stem Cell and Cancer Institute, Jl. A. Yani no 2. Pulo Mas, Jakarta, 13210, Indonesia, Microbiology Department, Faculty of Medicine, Maranatha Christian University, Bandung, 40164, Indonesia; Setiawan, M., Stem Cell and Cancer Institute, Jl. A. Yani no 2. Pulo Mas, Jakarta, 13210, Indonesia; Suyatna, F.D., Department of Pharmacology and Therapeutics, Medical Faculty, University of Indonesia, Jakarta, Indonesia; Japutri, I., Stem Cell and Cancer Institute, Jl. A. Yani no 2. Pulo Mas, Jakarta, 13210, Indonesia; Setiawan, B., Kalbe Farma Tbk. Jl. Let. Jend. Suprapto Kav 4. Cempaka Putih, Jakarta, 10510, Indonesia; Sandra, F., Stem Cell and Cancer Institute, Jl. A. Yani no 2. Pulo Mas, Jakarta, 13210, Indonesia
Aim Umbilical cord blood mononucleated (UCBMC) cells has been shown to be the stem cells originated from umbilical cord blood. To date, UCBMC has been introduced as an alternative source for stem cells used in autologous and allogeneic transplantations. Several clinical studies have demonstrated that UCBMCs required less stringent selection for HLA matches between donor and recipient with less cases of graft versus host reaction. In this study, UCBMCs are known to contain many stem cells, were characterized and compared to peripheral blood for their immunogenic profile. Method To elucidate the potential of UCBMC alloreactivity, mixed lymphocyte reaction (MLR) assay was performed. The donor and effectors cells were HLA-typed using PCR method to determine their alloreactivity. Further, to distinguish the level of HLA class I and II expression flowcytometry was done using monoclonal antibodies against those molecules. All the analyse were carried out on UCBMCs and peripheral blood mononucleated cells (PBMCs). Results The result of MLR assay showed that there was less IFN-γ secretion detected in the co-cultured medium in the presence of UCBMCs compared to PBMCs counterpart, indicating less possible rejection of UCBMC. Further, we found that there were only 1-3 alleles of HLA match (out of 8 alleles) among the PBMCs and UCBMCs. By using flowcytometry assay, we could further demonstrate lower HLA Class I expression level with less amount of HLA Class II expressing cells in UCBMC compared to those in PBMCs. Conclusion These findings clearly demonstrate the low immunogenicity of UCBMCs, based on the low level of secreted IFN-γ in the MLR assay, low expression level of HLA Class I, and small population of HLA Class II expressing cells. The outcomes from this study would raise a better understanding in the usage of umbilical cord blood as an alternative source of stem cells for allogeneic transplantation. © 2010, Faculty of Medicine, Universitas Indonesia. All rights reserved.
Immunogenicity; Stem cell; Umbilical cord blood
gamma interferon; HLA A antigen; HLA A02 antigen; HLA A04 antigen; HLA A14 antigen; HLA A22 antigen; HLA A32 antigen; HLA A33 antigen; HLA A34 antigen; HLA A35 antigen; HLA antigen; HLA antigen class 1; HLA antigen class 2; HLA B antigen; HLA B14 antigen; HLA B15 antigen; HLA B17 antigen; HLA B20 antigen; HLA B23 antigen; HLA B32 antigen; HLA B40 antigen; HLA B45 antigen; HLA B50 antigen; HLA B54 antigen; HLA DQB1 antigen; HLA DRB1 antigen; unclassified drug; allele; alloimmunity; allotransplantation; antigen expression; Article; controlled study; enzyme linked immunosorbent assay; flow cytometry; gene amplification; HLA typing; human; human cell; immunogenicity; mixed lymphocyte reaction; mononuclear cell; peripheral blood mononuclear cell; polymerase chain reaction; protein expression; u
Faculty of Medicine, Universitas Indonesia
08531773
Article
Q4