Publikasi Scopus FKUI Tahun 2010 s/d 2020 (data Per 3 Februari 2021)

Yasmon A., Yusmaniar, Karuniawati A., Bela B.
41462004500;57192910143;54886816200;24723637900;
Simultaneous detection of Legionella species and Legionella pneumophila by duplex PCR (dPCR) assay in cooling tower water samples from Jakarta, Indonesia
2010
Medical Journal of Indonesia
19
4
223
227
3
Department of Microbiology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
Yasmon, A., Department of Microbiology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Yusmaniar, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Karuniawati, A., Department of Microbiology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Bela, B., Department of Microbiology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
Aim: Since culture method is time-consuming and has low sensitivity, we developed a duplex PCR (dPCR) assay for the detection of Legionella sp. and L. pneumophila in cooling tower samples. We used culture method as a gold standard. Methods: Optimization of dPCR method was performed to obtain an assay with high sensitivity and specificity. The optimized method was used to detect Legionella sp. dan L. pneumophila in 9 samples obtained from 9 buildings in Jakarta. For culture method, the bacteria were grown or isolated on selective growth factor supplemented-buffered charcoal yeast extract (BCYE) media. Results: Of 9 samples tested by dPCR assay, 6 were positive for Legionella species,1 was positive for L. pneumophila, and 2 showed negative results. For the same samples, no Legionella sp. was detected by the culture method. Conclusion: dPCR assay was much more sensitive than the culture method and was potentially used as a rapid, specific and sensitive test for routine detection of Legionella sp. dan for L. pneumophila in water samples. © 2010, Faculty of Medicine, Universitas Indonesia. All rights reserved.
16S-rRNA gene; BCYE media; Mip gene
bacterial DNA; primer DNA; Article; bacterium culture; bacterium detection; colony forming unit; controlled study; cooling tower; DNA extraction; duplex polymerase chain reaction; Indonesia; Legionella pneumophila; nonhuman; polymerase chain reaction; process optimization; sensitivity and specificity; water analysis
Faculty of Medicine, Universitas Indonesia
08531773
Article
Q4