Publikasi Scopus FKUI Tahun 2010 s/d 2020 (data Per 3 Februari 2021)

Savitri I.K.E., Ibrahim F., Sahlan M., Wijanarko A.
54885346100;54886001500;57189182661;6506885367;
Rapid and efficient purification method of phospholipase A2 from Acanthaster planci
2011
International Journal of Pharma and Bio Sciences
2
2
401
406
8
Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Jl. Prof. Somantri Brojonegoro, Kampus UI, Beji, Depok 16424, Indonesia; Department of Aquatic Product Technology, Faculty of Fishery, Pattimura University, Jl. Ir. Martinus Putuhena, Kampus UnPatti, Poka, Ambon 97233, Indonesia; Research and Healthcare Service Center on Virology and Cancer Pathobiology, Faculty of Medicine, Universitas Indonesia, Kampus UI, Salemba, Jakarta 10430, Indonesia
Savitri, I.K.E., Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Jl. Prof. Somantri Brojonegoro, Kampus UI, Beji, Depok 16424, Indonesia, Department of Aquatic Product Technology, Faculty of Fishery, Pattimura University, Jl. Ir. Martinus Putuhena, Kampus UnPatti, Poka, Ambon 97233, Indonesia; Ibrahim, F., Research and Healthcare Service Center on Virology and Cancer Pathobiology, Faculty of Medicine, Universitas Indonesia, Kampus UI, Salemba, Jakarta 10430, Indonesia; Sahlan, M., Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Jl. Prof. Somantri Brojonegoro, Kampus UI, Beji, Depok 16424, Indonesia; Wijanarko, A., Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Jl. Prof. Somantri Brojonegoro, Kampus UI, Beji, Depok 16424, Indonesia
Rapid and efficient purification method of the phopholipase A2 (PLA2) from the crown-of-thorns starfish Acanthaster planci was done. It was developed without using separation column and taken less time around one day purified. Compare to common procedure that was done by purification measurement using several columns chromatography and was known taken several days, this purification method is more simplify. This purification method use only 30 minutes heat treatment on 60°C and then is fractionated by 20% saturated ammonium sulfate precipitation. The protein complex was analyzed by one dimensional sodium dodecylsulfate polyacrylamide gel electrophoresis. PLA2 is obtained an excellent overall yield with a final enzyme activity was similar to result of common procedure using four columns chromatography.
Phospholipase A2; Purification; Simple method; Starfish; Yield and activity
ammonium sulfate; phospholipase A2; Acanthaster planci; article; column chromatography; complex formation; controlled study; enzyme activity; enzyme assay; enzyme purification; fractionation; heat treatment; intermethod comparison; nonhuman; polyacrylamide gel electrophoresis; precipitation; process optimization; protein content; starfish; Acanthaster planci; Asteroidea
09756299
Article
Q4