No records
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125 |
Hermansyah T.A., Ginanjar E., Putri V.H. |
57428569000;23472616600;57428569100; |
Elevation of Cardiac Biomarkers in COVID-19 As a Major Determinant for Mortality: A Systematic Review |
2021 |
Acta medica Indonesiana |
53 |
4 |
|
385 |
396 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123568570&partnerID=40&md5=0047595d5dd32380033c242e7c79fe4c |
Faculty of Medicine Universitas IndonesiaJakarta, Indonesia |
Hermansyah, T.A., Faculty of Medicine Universitas IndonesiaJakarta, Indonesia; Ginanjar, E.; Putri, V.H. |
BACKGROUND: This study aimed to summarize the prognosis of Corona Virus Disease 2019 (COVID-19) patients with elevated troponin and N-terminal pro brain natriuretic peptide (NT-proBNP) levels and demonstrate the involvement of myocardial injury as a complication in COVID-19. METHODS: A systematic literature search was performed using several databases (PubMed, MEDLINE, PROQUEST and SCOPUS ) for studies published up to August 2020. Observational studies about the mortality outcome of COVID-19 patients who experienced cardiac injury, as defined by the elevation of serum levels of troponin, brain natriuretic peptide (BNP), with NT-proBNP or only BNP or only NT-proBNP, were included. In addition, a critical appraisal was conducted for all included studies using the Critical Appraisal for Prognostic Studies checklist published by the Centre for Evidence-Based Medicine by the University of Oxford. RESULTS: Seven retrospective observational studies fulfilled the inclusion criteria. This study found that there is a higher risk of death in COVID 19 patients with higher levels of troponin and NT-proBNP, indicating the importance of these biomarkers as determinant factors to predict in-hospital deaths. CONCLUSION: Based on the analysis, elevation of troponin and NT-proBNP levels plays an essential role in determining the patient prognosis because it is shown to be associated with in-hospital mortality. This also supports the involvement of myocardial injury as a prominent fatal complication in COVID-19. |
BNP; COVID-19; myocardial injury; NT-proBNP; prognostic factors; troponin |
biological marker; brain natriuretic peptide; peptide fragment; pro-brain natriuretic peptide (1-76); troponin; blood; human; mortality; observational study; prognosis; retrospective study; Biomarkers; COVID-19; Humans; Natriuretic Peptide, Brain; Observational Studies as Topic; Peptide Fragments; Prognosis; Retrospective Studies; SARS-CoV-2; Troponin |
NLM (Medline) |
01259326 |
|
35027485 |
Article |
Q3 |
321 |
14162 |
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126 |
Saldi S.R.F., Safitri E.D., Setiati S., Ranakusuma R.W., Marsigit J., Azwar M.K., Astuti P., Sari C.Y.I., Istanti R., Yulianti M., Rumende C.M., Yunihastuti E., Susilo A., Harimurti K., Liastuti L.D., Trimartani T., Restuti R.D., Syam A.F. |
55201904000;57195934356;14325991900;57189729404;57218912589;57202798959;57436688300;57218292263;23496653300;57216405885;14325966300;57221273925;57217867079;23473513200;57204676441;57428529000;55261428300;8443384400; |
Prognostic Scoring System for Mortality of Hospitalized COVID-19 Patients in Resource-Limited Settings: A Multicenter Study from COVID-19 Referral Hospitals |
2021 |
Acta medica Indonesiana |
53 |
4 |
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407 |
415 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123568238&partnerID=40&md5=e5654832599ee37e6d1d6dad0e5cc30c |
Clinical Epidemiology and Evidence-Based Medicine, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo HospitalJakarta, Indonesia |
Saldi, S.R.F., Clinical Epidemiology and Evidence-Based Medicine, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo HospitalJakarta, Indonesia; Safitri, E.D.; Setiati, S.; Ranakusuma, R.W.; Marsigit, J.; Azwar, M.K.; Astuti, P.; Sari, C.Y.I.; Istanti, R.; Yulianti, M.; Rumende, C.M.; Yunihastuti, E.; Susilo, A.; Harimurti, K.; Liastuti, L.D.; Trimartani, T.; Restuti, R.D.; Syam, A.F. |
BACKGROUND: Many studies identified the risk factors and prognostic factors related to in-hospital COVID-19 mortality using sophisticated laboratory tests. Cost and the availability of supporting blood tests may be problematic in resource-limited settings. This multicenter cohort study was conducted to assess the factors associated with mortality of COVID-19 patients aged 18 years and older, based on history taking, physical examination, and simple blood tests to be used in resource-limited settings. METHODS: The study was conducted between July 2020 and January 2021 in five COVID-19 referral hospitals in Indonesia. Among 1048 confirmed cases of COVID-19, 160 (15%) died during hospitalization. RESULTS: Multivariate analysis showed eight predictors of in-hospital mortality, namely increased age, chronic kidney disease, chronic obstructive pulmonary disease, fatigue, dyspnea, altered mental status, neutrophil-lymphocyte ratio (NLR) ≥ 5.8, and severe-critical condition. This scoring system had an Area-under-the-curve (AUC) of 84.7%. With cut-off score of 6, the sensitivity was 76.3% and the specificity was 78.2%. CONCLUSION: The result of this practical prognostic scoring system may be a guide to decision making of physicians and help in the education of family members related to the possible outcome. |
COVID-19; mortality; predictive score; prognostic; resource-limited settings |
clinical trial; comorbidity; health care planning; hospital; hospital mortality; human; mortality; multicenter study; patient referral; prognosis; retrospective study; sensitivity and specificity; Comorbidity; COVID-19; Health Resources; Hospital Mortality; Hospitals; Humans; Prognosis; Referral and Consultation; Retrospective Studies; SARS-CoV-2; Sensitivity and Specificity |
NLM (Medline) |
01259326 |
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35027487 |
Article |
Q3 |
321 |
14162 |
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127 |
Yunihastuti E. |
57221273925; |
Prioritizing Health Care Workers Safety: The International Year of Health and Care Workers 2021 |
2021 |
Acta medica Indonesiana |
53 |
4 |
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371 |
373 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123567858&partnerID=40&md5=6b031d0156ba2c68eb828650dd45d00d |
Division of Allergy and Clinical Immunology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo HospitalJakarta, Indonesia |
Yunihastuti, E., Division of Allergy and Clinical Immunology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo HospitalJakarta, Indonesia |
Healthcare workers pose a substantial risk of acquiring COVID-19 infection during their daily works. We have seen various conditions during the pandemic, such as limited adequate personal protective equipment (PPE), accurate diagnostic tests, lack of information regarding disease management, unsupportive work environment, and excessive workload, increased the number of HCWs-infected COVID-19. Compared to the general population, the risk of COVID-19 infection was several-fold higher in HCWs.Employers and health care workers both should share the responsibility to prevent occupationally acquired infections and avoid causing harm to patients by taking reasonable precautions to prevent vaccine-preventable disease transmission.This year, WHO has launched a year-long campaign under the theme -protect, invest, together'. It highlights the urgent need to invest in health care workers, not only during COVID-19. We need to ensure that all health care workers are supported, protected, motivated, and equipped to deliver safe health care at all times, to provide a high-quality standard of care to the patients. |
COVID-19; healthcare workers; personal protective equipment; safety; vaccination |
health care personnel; human; occupational health; pandemic; protective equipment; World Health Organization; COVID-19; Health Personnel; Humans; Occupational Health; Pandemics; Personal Protective Equipment; World Health Organization |
NLM (Medline) |
01259326 |
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35027483 |
Article |
Q3 |
321 |
14162 |
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128 |
Fadrian F., Chen K., Kumalawati J., Rumende C.M., Shatri H., Nelwan E.J. |
57268114000;57428620500;6504406695;14325966300;28767986500;14527452900; |
The Validation of Drug Resistance in Pneumonia (DRIP) Score in Predicting Infections due to Drug-Resistant Pathogens in Community-acquired Pneumonia at Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
2021 |
Acta medica Indonesiana |
53 |
4 |
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416 |
422 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123567110&partnerID=40&md5=9488cfa84614808f64df6510f7925f6d |
1. Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo Hospital, Indonesia. 2. Division of Tropical Medicine and Infectious Disease, Department of Internal Medicine, Faculty of Medicine, alas University - M. Djamil Hospital, Padang, Jakarta, Indonesia |
Fadrian, F., 1. Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo Hospital, Indonesia. 2. Division of Tropical Medicine and Infectious Disease, Department of Internal Medicine, Faculty of Medicine, alas University - M. Djamil Hospital, Padang, Jakarta, Indonesia; Chen, K.; Kumalawati, J.; Rumende, C.M.; Shatri, H.; Nelwan, E.J. |
BACKGROUND: The emergence of drug-resistant pathogens (DRP) in recent years possibly contributes to the common problems associated with community-acquired pneumonia. However, to predict the risk of the ailment, the DRIP score is mainly applied, although no validation study has been reported in Indonesia. Therefore, the score prediction accuracy in the population, patient characteristics and germ patterns appears indefinite, particularly for Cipto Mangunkusumo Hospital, Jakarta. The purpose of this study is to determine the DRIP performance as an instrument in predicting infections due to drug-resistant pathogens (DRP) in community-acquired pneumonia at Cipto Mangunkusumo Hospital. METHODS: This research employed a cross-sectional design, where the subjects were community-acquired pneumonia patients treated between January 2019 and June 2020. In addition, adequate medical records of the participants were obtained. The condition is defined as DRP when the sputum culture results show resistance to non-pseudomonal β-lactam antibiotics, macrolides, and respiratory fluoroquinolones. Furthermore, the score performance was analyzed by determining the calibration and discrimination values, using the Hosmer-Lemeshow test and AUROC, respectively. RESULTS: A total of 254 subjects were known to have satisfied the selection criteria. These participants were categorized into DRP and non-DRP groups, with 103 (40.6%) and 151 (59.4%) patients, correspondingly. The DRIP calibration analysis using the Hosmer-Lemeshow test obtained p-value = 0.001 (p <0.05), while an AUC value of 0.759 (CI 95%, 0.702-0.810) was derived from the ROC curve. However, at a score of ≥ 4, the DRIP showed sensitivity, specificity, positive and negative predictive values of 70.9, 92.7, 86.9, and 82.3%, respectively. CONCLUSION: The DRIP score demonstrated a significant performance in predicting infections due to DRP in community-acquired pneumonia. |
community-acquired pneumonia; DRIP score; resistant pathogens |
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NLM (Medline) |
01259326 |
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35027488 |
Article |
Q3 |
321 |
14162 |
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129 |
Nelwan E.J., Guterres H., Pasaribu A.I., Shakinah S., Limato R., Widodo D. |
14527452900;57223285438;57428543800;57428569400;57196224631;8231822900; |
The Comparison of Point Prevalence Survey (PPS) and Gyssens Flowchart Approach on Antimicrobial Use Surveillance in Indonesian National Referral Hospital |
2021 |
Acta medica Indonesiana |
53 |
4 |
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505 |
511 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123566730&partnerID=40&md5=0eb0821dbbca8a19666af97b0fdd062d |
1. Division of Tropical and Infectious Disease, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia, Indonesia 2. Infection and Immunology Research Cluster, Indonesian Medical Research Institute Faculty of Medicine Universitas Indonesia, Indonesia 3. Member of Antimicrobial Resistance Control Cipto Mangunkusumo HospitalJakarta, Indonesia |
Nelwan, E.J., 1. Division of Tropical and Infectious Disease, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia, Indonesia 2. Infection and Immunology Research Cluster, Indonesian Medical Research Institute Faculty of Medicine Universitas Indonesia, Indonesia 3. Member of Antimicrobial Resistance Control Cipto Mangunkusumo HospitalJakarta, Indonesia; Guterres, H.; Pasaribu, A.I.; Shakinah, S.; Limato, R.; Widodo, D. |
The antimicrobial resistance (AMR) rate in Indonesia is steadily rising, despite the existing national action plan in 2014. In line with the Global Action Plan on AMR, proper surveillance on antimicrobial usage and resistance are needed. At present, antimicrobial surveillance (AMS) data in Indonesia is heterogeneous, fragmented, and localized. The common method of antimicrobial surveillance (AMS) in referral hospitals is by implementing Gyssens flowchart during Antimicrobial Resistance Control Program Committee clinical rounds. However, the recent method of AMS with Point Prevalence Survey (PPS) offers many advantages include its concise and simple protocol, large data collection, shorter required time, comprehensive data outcomes, real-time data, and standardized parameters. In low-middle income countries such as Indonesia with its restricted resources in AMS, PPS is superior compared to the 'traditional' hospital clinical round in generating representative and homogenous outcomes that can be compared to data from other centers worldwide. |
antimicrobial; Gyssens Flowchart; Indonesia; Point Prevalence Survey |
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NLM (Medline) |
01259326 |
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35027501 |
Article |
Q3 |
321 |
14162 |
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130 |
Yulianti M., Johan C., Singh G., Tenda E.D., Herikurniawan H., Wijaya I.P.E.K. |
57216405885;57428554200;57209127773;57189692101;57428567200;57221504958; |
Effectiveness of Convalescent Plasma Therapy in Treating COVID-19: an Evidence-based Case Report |
2021 |
Acta medica Indonesiana |
53 |
4 |
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497 |
504 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123566521&partnerID=40&md5=3ff22c5c237f932569b8171a1ff5221b |
Department of Internal Medicine, Faculty of Medicine Universitas IndonesiaJakarta, Indonesia |
Yulianti, M., Department of Internal Medicine, Faculty of Medicine Universitas IndonesiaJakarta, Indonesia; Johan, C.; Singh, G.; Tenda, E.D.; Herikurniawan, H.; Wijaya, I.P.E.K. |
BACKGROUND: Convalescent plasma is a potentially beneficial, tolerable, and available additional treatment option for COVID-19. This study aims to evaluate whether the administration of convalescent plasma therapy leads to improved clinical outcomes in COVID-19 patients compared to standard medical therapy. METHODS: We conducted a search of Pubmed, Cochrane, and EBSCO for studies assessing the clinical question using inclusion and exclusion criteria. Selected studies were critically appraised, and the results were summarized. RESULTS: A meta-analysis of 10 randomized clinical trials (RCTs), an RCT, a case-control clinical study were selected and assessed. Only the case-control clinical study showed that convalescent plasma administration improved the clinical outcomes of patients with COVID-19, including all-cause mortality, hospital length of stay, and the need for mechanical ventilation. On the contrary, the other two studies of a higher level of evidence showed no significant clinical outcome improvement with convalescent plasma therapy. CONCLUSION: The effectiveness of convalescent plasma therapy in improving clinical outcomes of patients with COVID-19 was still inconclusive due to several study limitations and other possible causes. |
convalescent plasma; coronavirus; COVID-19; SARS-CoV-2 |
artificial ventilation; human; meta analysis; passive immunization; randomized controlled trial (topic); therapy; treatment outcome; COVID-19; Humans; Immunization, Passive; Randomized Controlled Trials as Topic; Respiration, Artificial; SARS-CoV-2; Treatment Outcome |
NLM (Medline) |
01259326 |
|
35027500 |
Article |
Q3 |
321 |
14162 |
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131 |
Maulahela H., Fauzi A. |
57189612709;36518523000; |
Peripancreatic Tuberculosis Lymphadenopathy: The Role of Endoscopic Ultrasound for Diagnosis |
2021 |
Acta medica Indonesiana |
53 |
4 |
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457 |
459 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123566183&partnerID=40&md5=db2cd4e2d6a90740d0bc3e39263c3c1d |
Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo HospitalJakarta, Indonesia |
Maulahela, H., Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo HospitalJakarta, Indonesia; Fauzi, A. |
Pancreatic and peripancreatic tuberculosis is a rare abdominal tuberculosis. Diagnosis for pancreatic tuberculosis can be challenging. Conventional imaging tools may show mass or malignancy in the pancreas. Endoscopic ultrasound (EUS) is an excellent tools for evaluating pancreas and peri pancreas region. It also allows us to obtain tissue sample for cytology and histopathology. Here we present a case of peripancreatic tuberculosis lymphadenopathy that mimic pancreatic mass. His symptoms were also nonspecific (weight loss, epigastric pain, and irregular fever). From EUS evaluation we found that there was no mass but multiple lymphadenopathy around the pancreas and then performed FNA. The result of the cytology was granuloma inflammation and caseous necrosis which is compatible with tuberculosis infection. From this case illustration we conclude that EUS is an important diagnostic tool for pancreatic lesion to avoid unnecessary surgery. |
endoscopic ultrasound; lymphadenopathy; Pancreas; tuberculosis |
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NLM (Medline) |
01259326 |
|
35027493 |
Article |
Q3 |
321 |
14162 |
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132 |
Rajabto W., Priantono D., Mulyadi R. |
36519576100;57219443427;56403164500; |
Pulmonary Embolism in Hospitalized Patient with Coronavirus Disease 2019 (COVID-19) |
2021 |
Acta medica Indonesiana |
53 |
4 |
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493 |
496 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123461861&partnerID=40&md5=389908dacd77633e6cd2254227617a8c |
Division of Hematology-Medical Oncology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo General HospitalJakarta, Indonesia |
Rajabto, W., Division of Hematology-Medical Oncology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo General HospitalJakarta, Indonesia; Priantono, D.; Mulyadi, R. |
Coronavirus disease 2019 (COVID-19) has been a global pandemic for over a year. Meanwhile, thrombosis occurs in up to one-third of hospitalized patients with the disease, while pulmonary embolism has been reported to be the most dangerous thrombosis which greatly increases mortality in COVID-19.Hospitalized patients with COVID-19 are at high risk of thromboembolic complications such as deep vein thrombosis and pulmonary embolism. The hypercoagulable state caused by COVID-19 leads to activation of coagulation cascade, meanwhile, CT pulmonary angiography is used to diagnose or exclude pulmonary embolism. Furthermore, ground-glass opacities are also evaluated using this modality. Low molecular weight heparin is the anticoagulant of choice due to simplicity in administration and low risk of drug-drug interactions.Pulmonary embolism occurs in COVID-19 patients without DVT. Based on the results, parenteral anticoagulant followed by DOAC is the mainstay of treatment in COVID-19 coagulopathy. |
COVID-19; pulmonary embolism; thrombosis |
anticoagulant agent; complication; diagnostic imaging; human; lung embolism; vein thrombosis; virology; Anticoagulants; COVID-19; Humans; Pulmonary Embolism; SARS-CoV-2; Venous Thrombosis |
NLM (Medline) |
01259326 |
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35027499 |
Article |
Q3 |
321 |
14162 |
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133 |
Shatri H., Prasetyaningtyas A., Putranto R., Rinaldi I. |
28767986500;57426347900;56074051000;23475122400; |
Palliative Prognostic Index Validation in Hospitalized Advanced Cancer Patients in Indonesia Tertiary Hospitals |
2021 |
Acta medica Indonesiana |
53 |
4 |
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442 |
449 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123460876&partnerID=40&md5=6a2a641fbdfa3aa8f7eb17fb4fc4458e |
1. Division of Psychosomatic and Pallliative, Indonesia. 2. Clinical Epidemiology Unit, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - dr. Cipto Mangunkusumo HospitalJakarta, Indonesia |
Shatri, H., 1. Division of Psychosomatic and Pallliative, Indonesia. 2. Clinical Epidemiology Unit, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - dr. Cipto Mangunkusumo HospitalJakarta, Indonesia; Prasetyaningtyas, A.; Putranto, R.; Rinaldi, I. |
BACKGROUND: Accurate prediction of survival is important for advanced cancer patients to determine medical interventions plan the patient's lives and prepare for their death. The palliative prognostic index (PPI) is most popular scores used worldwide to predict life expectancy in advanced cancer palliative patients. The purpose of this study was to test validity and the performance of PPI in Cipto Mangunkusumo Hospital as a Tertiary Referral Nasional Hospital. METHODS: This retrospective cohort study, uses total subject during study with consecutive sampling. Palliative prognostic index was assessed by a palliative care team (PCT). Demographic data were summarized as n (%) and Chi square for categorical variables and median or mean for continuous variables. Overall survival was calculated using the Kaplan-Meier method with hazard ratios. The performance of PPI analyzed using SPSS version 20.0, includes for Receiving Operator Characteristics (ROC) and Hosmer-Lemeshow calibration test. RESULTS: 160 patients were included in the PPI study. The subjects have an average age of 50.08 years and are mostly women 68.10%. 28 (17.50%) had symptoms of dyspnoea, 22 (14.60%) pneumonia, and 19 (11.90%) had pain. The number of patients who died during hospitalisation was 83 (51.90%). PPI sum score >6 109 (68,10%). Calibration performance PPI score reached x2 = 8.915 (p = 0.259), and showed correlation r 0.799 (p 0.000). The accuracy of PPI scores in predicting survival in advanced cancer patients in studies for survival <3 weeks 81%, with a sensitivity of 85%, specificity 70%, PPV 86%, and NPV 67%. Predictive accuracy of survival within 3-6 weeks had 76%, sensitivity 66%, specificity 88%, PPV 85% and NPV 70%. PPI score discrimination performance is had a AUC value of 0.822 (95% CI 0.749-0.895). CONCLUSION: Palliative Prognostic Index (PPI ) is valid and has good performance in predicting the survival of advanced cancer patients and may be used to help clinicians in palliative care consultation. |
Cancer; palliative prognostic index (PPI); tertiary hospital; validation |
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NLM (Medline) |
01259326 |
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35027491 |
Article |
Q3 |
321 |
14162 |
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135 |
Pujianto D., Permatasari S. |
8745734300;57408686700; |
Mouse CD52 is predominantly expressed in the cauda epididymis, regulated by androgen and lumicrine factors |
2021 |
Journal of Human Reproductive Sciences |
14 |
4 |
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350 |
355 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122693572&doi=10.4103%2fjhrs.jhrs_29_21&partnerID=40&md5=442cc066428b3f1038737d1f1d81f1cc |
Department of Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, 10430, Indonesia; Department of Biology, Faculty of Medicine, Universitas Palangka Raya, Indonesia |
Pujianto, D., Department of Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, 10430, Indonesia; Permatasari, S., Department of Biology, Faculty of Medicine, Universitas Palangka Raya, Indonesia |
Background: Sperm maturation takes place through contact between sperm and proteins produced in the epididymal lumen. CD52 had been characterised in the sperm; however, the expression and its regulation in the epididymis are mostly unknown. Aim: This study aimed to analyse the expression and regulation of CD52 in the mouse epididymis. Setting and Design: Experimental design was used in this study. Materials and Methods: Epididymis tissues from mice strain Deutch Democratic Yokohama were used as sources of total RNA. Bioinformatic tool was used to predict signal peptides. Quantitative real-Time reverse transcription-polymerase chain reaction was used to analyse tissue distribution, androgen, testicular factors dependency and postnatal development. Statistical Analysis: One-way analysis of variance was used to analyse differences between treatment and control untreated group. P < 0.05 was determined as a significant difference. Results: CD52 amino acid sequence contains a signal peptide, indicating it is a secretory protein. CD52 exhibited region-specific expression in the epididymis, with the highest level being in the cauda. CD52 expression was regulated by androgen indicated by a significant downregulation at day 1 and day 3 following a castration (P < 0.05). Dependency on androgen was confirmed by injection of exogenous testosterone which prevented downregulation by 50%. Moreover, lumicrine factors also influenced CD52 expression indicated by ligation of efferent duct which also reduced expression at day 1 to day 5 following the ligation (P < 0.05). CD52 expression was developmentally regulated. This was shown by increase in the level of expression starting at day 15 postnatally. Conclusion: CD52 shows characteristics of genes involved in sperm maturation in the epididymis. © 2021 Wolters Kluwer Medknow Publications. All rights reserved. |
Androgen; CD52; Epididymis; Lumicrine; Sperm maturation |
androgen; bromethol; CD52 antigen; hormone; lumicrine; RNA; signal peptide; testosterone; unclassified drug; amino acid sequence; androgen therapy; animal experiment; animal tissue; antigen expression; Article; castration; cauda epididymis; controlled study; down regulation; epididymis; male; mouse; nonhuman; postnatal development; quantitative analysis; real time reverse transcription polymerase chain reaction; spermatozoon maturation; tissue distribution |
Wolters Kluwer Medknow Publications |
09741208 |
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Article |
Q3 |
484 |
10359 |
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