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741 |
Sandora N., Putra M.A., Busro P.W., Ardiansyah, Muttaqin C., Makdinata W., Fitria N.A., Kusuma T.R. |
57204103434;57215605850;57192273817;57223036386;57226442135;57223405655;57148498800;57222897694; |
Preparation of Cell-Seeded Heart Patch In Vitro; Co-Culture of Adipose-Derived Mesenchymal Stem Cell and Cardiomyocytes in Amnion Bilayer Patch |
2021 |
Cardiovascular Engineering and Technology |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111590228&doi=10.1007%2fs13239-021-00565-4&partnerID=40&md5=cd6477f9e1ad4b1bf0110d68c68a71fc |
Institute of Medical Education and Research Indonesia, Jakarta, 10430, Indonesia; Department of Thoracic Surgery, RSCM, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Sandora, N., Institute of Medical Education and Research Indonesia, Jakarta, 10430, Indonesia; Putra, M.A., Department of Thoracic Surgery, RSCM, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Busro, P.W., Department of Thoracic Surgery, RSCM, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Ardiansyah, Department of Thoracic Surgery, RSCM, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Muttaqin, C., Department of Thoracic Surgery, RSCM, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Makdinata, W., Department of Thoracic Surgery, RSCM, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Fitria, N.A., Institute of Medical Education and Research Indonesia, Jakarta, 10430, Indonesia; Kusuma, T.R., Institute of Medical Education and Research Indonesia, Jakarta, 10430, Indonesia |
Introduction: Cardiovascular disease is the second killer across the globe, while coronary disease is the major cause. Cell therapy is one alternative to regenerate the infarcted heart wall. Materials and Methods: In this study, the cardiomyogenesis capacity of human adipose stem cells (hAdSC) and human cardiomyocytes (hCardio) cultured in a 3-D biological scaffold (decellularised amnion bilayer) for nine days in a static condition was investigated. The cardiomyogenesis capacity of hAdSC were identified using immunohistochemistry and RT-PCR. The population of the cells isolated from the heart tissue expressed cTnT-1 (13.38 ± 11.38%), cKit (7.85 ± 4.2%), ICAM (85.53 ± 8.69%), PECAM (61.63 ± 7.18%) and VCAM (35.9 ± 9.11%), while from the fat tissue expressed the mesenchymal phenotypes (CD73, CD90, CD105, but not CD45, CD34, CD11b, CD19 and HLA-DR). Two age groups of hAdSC donors were compared, the youngsters (30-40yo) and the elderly (60–70 yo). Results: The co-culture showed that after 5-day incubation, the seeded graft in the hAdSC-30 group had a tube-like appearance while the hAdSC-60 group demonstrated a disorganised pattern, despite of the MSC expressions of the hAdSC-60 were significantly higher. Initial co-culture showed no difference of ATP counts among all groups, however the hAdSC-30 group had the highest ATP count after 9 days culture (p = 0.004). After normalising to the normal myocardium, only the hAdSC-60 group expressed cTnT and MHC, very low, seen during the initial cultivation, but then disappeared. Meanwhile, the hAdSC-30 group expressed α-actinin, MHC and cTnT in the Day-5. The PPAR also was higher in the Day-5 compared to the Day-9 (p < 0.005). Conclusion: Cardiomyogenesis capacity of hAdSC co-cultured with hCardio in a 3-D scaffold taken from the 30–40yo donor showed better morphology and viability than the 60-70yo group, but maintained less than 5 days in this system. © 2021, Biomedical Engineering Society. |
3-D scaffold; Amnion bilayer; Human adipose stem cells; Human cardiomyocytes; Myocardial infarction |
Cell culture; Heart; Stem cells; Tissue; Adipose-derived mesenchymal stem cells; Biological scaffolds; Cardio-vascular disease; Cardiomyogenesis; Coronary disease; Human adipose stem cells; Immunohistochemistry; Static conditions; Scaffolds (biology) |
Springer |
1869408X |
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Article |
Q3 |
541 |
9343 |
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742 |
Paramaiswari W.T., Sidik N.S., Khoeri M.M., Tafroji W., Said W.F., Safari D. |
57221097580;57226416343;55994827400;57118271600;57196217927;23493586700; |
Isolation and Identification of Optochin-Resistant Viridans Group Streptococci from the Sputum Samples of Adult Patients in Jakarta, Indonesia |
2021 |
International Journal of Microbiology |
2021 |
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6646925 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111490612&doi=10.1155%2f2021%2f6646925&partnerID=40&md5=b022c6984c9b9cf061564ebeaeeb4831 |
Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Department of Biochemistry, Bogor Agricultural University, Bogor, Indonesia; Department of Clinical Pathology, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
Paramaiswari, W.T., Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Sidik, N.S., Department of Biochemistry, Bogor Agricultural University, Bogor, Indonesia; Khoeri, M.M., Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Tafroji, W., Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Said, W.F., Department of Clinical Pathology, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Safari, D., Eijkman Institute for Molecular Biology, Jakarta, Indonesia |
Aim. To investigate optochin-resistant viridans group streptococci (VGS) strains isolated from the sputum sample of adult patients with different clinical symptoms. Materials and Methods. Optochin-resistant VGS isolates were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). recA sequencing was used to confirm identified isolates at the genus level by MALDI-TOF MS. Finding. We identified 79% of tested isolates (148/187) at the species-level identification using the MALDI-TOF MS tool. We identified that the most common species isolated from sputum specimens were S. oralis (44.9%) followed by S. mitis (25.7%), S. infantis (9.1%), S. parasanguinis (7.5%), S. peroris (3.7%), S. anginosus (2.7%), and S. sanguinis (2.1%). Discussion. The S. oralis strains were majority of optochin-resistant VGS isolates obtained from sputum of adult patients in Jakarta, Indonesia. MALDI-TOF MS showed potential for the rapid identification tool to identify optochin-resistant VGS isolates. Although there were discrepancies in identifying isolates at the genus/species level, the performance could be improved by expanding its database. © 2021 Wisiva Tofriska Paramaiswari et al. |
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Hindawi Limited |
1687918X |
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Article |
Q3 |
696 |
7161 |
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743 |
Soeroso N.N., Ananda F.R., Pradana A., Tarigan S.P., Syahruddin E., Noor D.R. |
57201670271;57201666959;57202578193;6602815057;6507688750;57214096801; |
The absence of mutations in the exon 2 kras gene in several ethnic groups in north sumatra may not the main factor for lung cancer |
2021 |
Acta Informatica Medica |
29 |
2 |
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108 |
112 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111477707&doi=10.5455%2fAIM.2021.29.108-112&partnerID=40&md5=4854ca99a072394cbd039b5f66172820 |
Thoracic Oncology Division, Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara, Indonesia; Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara, Indonesia; Thoracic Oncology Division, Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Indonesia; Human Cancer Research Center, Indonesian Medical Education and Research Institute, Universitas Indonesia, Indonesia |
Soeroso, N.N., Thoracic Oncology Division, Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara, Indonesia; Ananda, F.R., Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara, Indonesia; Pradana, A., Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara, Indonesia; Tarigan, S.P., Thoracic Oncology Division, Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara, Indonesia; Syahruddin, E., Thoracic Oncology Division, Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Indonesia, Human Cancer Research Center, Indonesian Medical Education and Research Institute, Universitas Indonesia, Indonesia; Noor, D.R., Human Cancer Research Center, Indonesian Medical Education and Research Institute, Universitas Indonesia, Indonesia |
Background: Rat Sarcoma (RAS) protein encoded Guanosine Triphosphate (GTP-ase) activity, known as a switch of cell proliferation. The mutation of this protein alters the early stage of carcinogenesis and along with the interaction with other oncogene drivers and environmental factors affect the clinical characteristics and prognosis in cancer patients, particularly lung cancer. Objective: This study aims to determine the Kristen Rat Sarcoma (KRAS) mutation in lung cancer patients in North Sumatera and evaluate factors that might contribute in the development of lung cancer in the absence of KRAS mutation. Methods: This was a retrospective cohort study enrolled 44 subjects age > 18 year with the diagnosis of lung cancer. Histopathology preparation was obtained from surgery, bronchoscopy, and percutaneus needle biopsy then formed as paraffin-block. KRAS mutation was analyzed using Polymerase Chain Reaction (PCR) method with specific primer of exon 2 for evaluating the expression of RAS protein then continued with Sanger Sequencing Method at 12th and 13th codon. Results: The majority of subjects were male, age > 40 years old, bataknese, heavy smoker, with Adenocarcinoma. Almost all the subjects showed the expression of exon 2 of RAS protein in PCR examinations. However, Sequencing analysis using Bioedit Software, BLASTs and Finch T showed GGT GGC as protein base 219-224 which represented 12th and 13th Codon 12 and 13. The results interpreted there was no mutations of exon 2 of KRAS in North Sumatera Population. Conclusion: The absence of KRAS mutation in exon 2 in several ethnics in North Sumatera populations was not the main factors of lung cancer. © 2021 Noni Novisari Soeroso, Fannie Rizki Ananda, Andika Pradana, Setia Putra Tarigan, Elisna Syahruddin, Dimas Ramadhian Noor |
Ethnic variation; Exon 2; Indonesian; KRAS mutation; Lung cancer |
K ras protein; adult; age; Article; bronchoscopy; cancer diagnosis; cancer patient; cancer surgery; clinical article; cohort analysis; controlled study; ethnic difference; female; gender; gene expression; gene mutation; histopathology; human; lung adenocarcinoma; lung cancer; male; needle biopsy; polymerase chain reaction; retrospective study; Sanger sequencing; smoking |
Avicena Publishing |
03538109 |
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Article |
Q3 |
267 |
15980 |
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744 |
Rinaldi I., Sari R.M., Tedhy V.U., Winston K. |
23475122400;57208474079;57226362042;57224165400; |
Leukapheresis does not improve early survival outcome of acute myeloid leukemia with leukostasis patients – A dual-center retrospective cohort study |
2021 |
Journal of Blood Medicine |
12 |
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623 |
633 |
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1 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111261844&doi=10.2147%2fJBM.S312140&partnerID=40&md5=ef2e5f0e39b0ea908c07e71bba0bf1a4 |
Division of Hematology and Medical Oncology, Department of Internal Medicine, Cipto Mangunkusumo National General Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Division of Hematology and Medical Oncology, Department of Internal Medicine, Dharmais National Cancer Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Rinaldi, I., Division of Hematology and Medical Oncology, Department of Internal Medicine, Cipto Mangunkusumo National General Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Sari, R.M., Division of Hematology and Medical Oncology, Department of Internal Medicine, Dharmais National Cancer Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Tedhy, V.U., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Winston, K., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Introduction: Leukostasis is a medical emergency with high mortality which often occurs in acute myeloid leukemia patients with hyperleukocytosis. One of the therapies that can be used for leukostasis in acute myeloid leukemia is leukapheresis. However, whether leukapheresis can provide better survival benefit when compared with patients not receiving leukapheresis is still unclear. Hence, we aimed to evaluate the effect of chemotherapy plus leukapheresis combination versus chemotherapy only on 28-day survival of acute myeloid leukemia patients with leukostasis. Methods: This study was a dual-center retrospective cohort using secondary data from medical records collected from November 2018 to March 2019. Inclusion criteria were adult patients aged 18 years old or above, diagnosed with acute leukemia with hyperleukocytosis status defined by WBC count greater than 100,000/uL, and with symptoms of leukostasis. One-month survival analysis was conducted using Kaplan–Meier curve method. Univariate and multivariate analyses were then conducted using Cox proportional hazards model to obtain value of hazard ratio (HR) with a 95% confidence interval (CI). Results: A total of 38 patients were obtained for analysis. The median overall survival was 25 days (95% CI: 17.001–32.999 days) in the chemotherapy only group and 20 days (95% CI: 1.497–38.503) in the chemotherapy with leukapheresis group. The use of leukapheresis did not affect 28-day survival (HR: 1.140; 95% CI: 0.396–3.283; p value: 0.809) and 7-day survival (HR: 1.073; 95% CI: 0.277–4.152; p value: 0.919). In the multivariate analysis, age ≥60 years, blast percentage ≥90%, creatinine ≥1.4 mg/dL, and presence of disseminated intravascular coagulation were associated with worse 28-day survival. Conclusion: AML patients with leukostasis who received both chemotherapy and leuka-pheresis did not have better 28-day survival and 7-day survival when compared with patients receiving chemotherapy only. Old age, high blast percentage, high creatinine, and presence of disseminated intravascular coagulation were prognostic factors for worse 28-day survival. © 2021 Rinaldi et al. |
Chemotherapy; Hyperleukocytosis; Leukapheresis; Leukemia; Leukostasis; Survival |
creatinine; gluconate calcium; hemoglobin; lactate dehydrogenase; vascular cell adhesion molecule 1; acute myeloid leukemia; adult; allogeneic hematopoietic stem cell transplantation; Article; blood transfusion; cancer prognosis; chronic myeloid leukemia; clinical article; cohort analysis; de novo acute myeloid leukemia; dyspnea; female; heart failure; human; hypertension; hypocalcemia; Kaplan Meier method; leukapheresis; leukocyte count; leukocytosis; leukostasis; lymphocyte count; male; mean corpuscular hemoglobin; mean corpuscular volume; mean platelet volume; observational study; overall survival; platelet count; platelet lymphocyte ratio; pneumothorax; red blood cell distribution width; retrospective study; risk factor; thrombocyte aggregation |
Dove Medical Press Ltd |
11792736 |
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Article |
Q3 |
676 |
7401 |
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745 |
Syam A.F. |
8443384400; |
Gastrointestinal disorders in covid-19 patients: A great imitator |
2021 |
Medical Journal of Indonesia |
30 |
2 |
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166 |
169 |
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1 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111112690&doi=10.13181%2fmji.bc.204960&partnerID=40&md5=1e55d25030cd22add972a3dac84c5ac5 |
Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
Syam, A.F., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
Up to this point, the coronavirus disease 2019 (COVID-19) pandemic is still ongoing. Some studies with a large number of cases have reported its clinical manifestations, concluding that the disease is a great imitator. Patients may present with symptoms other than the main symptoms of respiratory tract infections, such as diarrhea, abdominal pain, nausea, or vomiting, which may sometimes cause a delayed treatment in managing COVID-19 patients. Reports of various hospitals have also demonstrated gastrointestinal complaints as a clinical manifestation in those patients. The patients may come with gastrointestinal symptoms as their early clinical manifestation, or the gastrointestinal symptoms may be found in hospitalized COVID-19 patients, which indeed can be explained since the SARS-CoV-2, an etiologic agent of COVID-19 infection, can obviously be found along the gastrointestinal tract. Hence, the virus can be found in fecal and anal, and therefore, rectal swabs can be used as a diagnostic tool for COVID-19 infection. © 2021 Author. |
COVID-19; Gastrointestinal disease; SARS-CoV-2 |
alkaline phosphatase; angiotensin converting enzyme 2; aspartate aminotransferase; transmembrane protease serine 2; virus RNA; anal swab; Article; coronavirus disease 2019; coughing; diabetes mellitus; diarrhea; dyspnea; epigastric pain; feces analysis; fever; gastrointestinal disease; gastrointestinal symptom; gastrointestinal tract; human; hypertension; inflammatory bowel disease; lung lavage; nasopharyngeal swab; nausea and vomiting; nonhuman; pneumonia; real time reverse transcription polymerase chain reaction; rectal swab; RNA virus; Severe acute respiratory syndrome coronavirus 2; stomach pain; thorax radiography; virus transmission |
Faculty of Medicine, Universitas Indonesia |
08531773 |
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Article |
Q4 |
164 |
21905 |
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746 |
Schuwirth L., Findyartini A. |
7003825152;56543777300; |
Never waste a good crisis: Resilient health professions education |
2021 |
Asia Pacific Scholar |
6 |
3 |
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1 |
4 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111073193&doi=10.29060%2fTAPS.2021-6-3%2fEV6N3&partnerID=40&md5=9a31005ce32498af7be04ec902845ab8 |
Prideaux Centre for Research in Health Professions Education, College of Medicine and Public Health, Flinders University, Australia; Department of Medical Education, Faculty of Medicine, Universitas Indonesia, Indonesia |
Schuwirth, L., Prideaux Centre for Research in Health Professions Education, College of Medicine and Public Health, Flinders University, Australia; Findyartini, A., Department of Medical Education, Faculty of Medicine, Universitas Indonesia, Indonesia |
[No abstract available] |
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National University of Singapore, Faculty of Law |
24249335 |
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Editorial |
#N/A |
#N/A |
#N/A |
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747 |
Rahyussalim A.J., Sahputra R.E., Yanwirasti, Manjas M., Whulanza Y., Kurniawati T., Aprilya D., Zufar M.L.L. |
55212166100;57212460235;56711920400;24473177100;36807053500;55213290600;57193710642;57216349850; |
The effect of mesenchymal stem cell-enriched scaffolds on mmp-8 and tgf-β levels of vertebrae postlaminoplasty in rabbit model |
2021 |
Stem Cells and Cloning: Advances and Applications |
14 |
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27 |
37 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111052490&doi=10.2147%2fSCCAA.S314107&partnerID=40&md5=6b23d4e3b167216975366ce0f5f83ae6 |
Department of Orthopedics and Traumatology Clinics, Faculty of Medicine, Universitas of Indonesia-Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Stem Cell and Tissue Engineering Cluster, IMERI Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Department of Surgery, Faculty of Medicine, Universitas Andalas-RSUP M. Djamil, Padang, Indonesia; Department of Anatomy, Faculty of Medicine, Universitas Andalas-RSUP M. Djamil, Padang, Indonesia; Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Jakarta, Indonesia |
Rahyussalim, A.J., Department of Orthopedics and Traumatology Clinics, Faculty of Medicine, Universitas of Indonesia-Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Stem Cell and Tissue Engineering Cluster, IMERI Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Sahputra, R.E., Department of Surgery, Faculty of Medicine, Universitas Andalas-RSUP M. Djamil, Padang, Indonesia; Yanwirasti, Department of Anatomy, Faculty of Medicine, Universitas Andalas-RSUP M. Djamil, Padang, Indonesia; Manjas, M., Department of Surgery, Faculty of Medicine, Universitas Andalas-RSUP M. Djamil, Padang, Indonesia; Whulanza, Y., Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Jakarta, Indonesia; Kurniawati, T., Stem Cell and Tissue Engineering Cluster, IMERI Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Aprilya, D., Department of Orthopedics and Traumatology Clinics, Faculty of Medicine, Universitas of Indonesia-Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Zufar, M.L.L., Department of Orthopedics and Traumatology Clinics, Faculty of Medicine, Universitas of Indonesia-Cipto Mangunkusumo General Hospital, Jakarta, Indonesia |
Introduction: Some laminoplasty procedures still have restenosis because of bony-bridging failure of the laminar hinge. The present study aimed to determine the effect of mesenchymal stem cell (MSC)-enriched scaffolds on vertebral regeneration after laminoplasty on the basis of the number of osteoblasts, matrix metalloproteinase-8 (MMP-8), and transforming growth factor-beta (TGF-β) levels. Methods: Laminoplasty procedure using the Hirabayashi technique was conducted at the lumbar level in 32 rabbits that were divided into four and three groups of the control (C) and treatment groups, respectively, with different types of laminoplasty spacer (T1, autograft; T2, scaffold; and T3, scaffold with MSCs). Histopathological studies were conducted to calculate the number of osteoblasts and enzyme-linked immunosorbent assay tests to detect MMP-8 and TGF-β 4 weeks after the surgery. Results: The results showed a significant decrease in MMP-8 level in the T3 group compared with that in the control group (p < 0.05). A significant difference exists between the average number of newly formed osteoblasts in the control group compared with that in the T3 group (p < 0.05) with a higher mean blood TGF-β level of all experimental groups compared with that of the control group (p = 0.58). Conclusion: The significant decrease in MMP-8 levels, increase in TGF-β levels, and increased number of osteoblasts on MSC-seeded polylactic acid scaffolds could be useful to support the laminoplasty procedure to prevent restenosis because it was biocompatible and promoted the bone healing process. © 2021 Rahyussalim et al. |
Hirabayashi technique; Laminoplasty; Mesenchymal stem cells; Scaffolds |
enrofloxacin; eosin; hematoxylin; ketoprofen; liquid nitrogen; neutrophil collagenase; polylactic acid; transforming growth factor beta; animal experiment; animal model; animal tissue; Article; autograft; blood sampling; body weight; bone development; cell suspension; cell viability; cervical spine dislocation; controlled study; cryopreservation; enzyme linked immunosorbent assay; follow up; fracture healing; histopathology; inflammation; injury; laminoplasty; mesenchymal stem cell; neoplasm; nonhuman; nutrition; ossification; osteoblast; restenosis; sample size; spine; umbilical cord |
Dove Medical Press Ltd |
11786957 |
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Article |
Q2 |
606 |
8341 |
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748 |
Karina K., Ekaputri K., Biben J.A., Purwoko R.H., Sibuea T.P., Astuti S.L., Loho A.M., Limengka Y., Nelfidayani, Agustini S., Krisandi G., Maryam A., Rosadi I., Rosliana I., Sobariah S., Subroto W.R., Afini I., Widyastuti T., Zakiyah A., Ernanda D., Aini N., Jusryanti, Sulaeha A.D., Prestiani S.I., Donna I.M., Habibi, Mutiara M.S. |
57212146678;57285088000;55947833300;57286031400;57286487800;57285797400;57223264641;37029815800;57286487900;57286488000;57225009844;57286260300;57204720098;57186671400;57203547382;57218940654;57211068324;57211074403;57226293808;57226273639;57226289464;57286944600;57226286645;57283770100;57284210300;57285797500;57284210400; |
Evaluating the Safety of Intravenous Delivery of Autologous Activated Platelet-rich Plasma |
2021 |
Journal of Health Sciences |
11 |
2 |
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61 |
65 |
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2 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111033469&doi=10.17532%2fjhsci.2021.1276&partnerID=40&md5=b0a026ecaccc92d972564c5dfd1f4824 |
Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Hayandra Lab, Yayasan Hayandra Peduli, Jakarta, Indonesia; Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Jakarta, Indonesia; Pusat Kajian Stem Cell, Universitas Pembangunan Nasional Veteran Jakarta, Jakarta, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Indonesia |
Karina, K., Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia, Hayandra Lab, Yayasan Hayandra Peduli, Jakarta, Indonesia, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia, Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Jakarta, Indonesia, Pusat Kajian Stem Cell, Universitas Pembangunan Nasional Veteran Jakarta, Jakarta, Indonesia; Ekaputri, K., Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Biben, J.A., Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Purwoko, R.H., Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Sibuea, T.P., Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Astuti, S.L., Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Loho, A.M., Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Limengka, Y., Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Nelfidayani, Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Agustini, S., Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Krisandi, G., Hayandra Lab, Yayasan Hayandra Peduli, Jakarta, Indonesia, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Maryam, A., Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Rosadi, I., Hayandra Lab, Yayasan Hayandra Peduli, Jakarta, Indonesia, Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Indonesia; Rosliana, I., Hayandra Lab, Yayasan Hayandra Peduli, Jakarta, Indonesia; Sobariah, S., Hayandra Lab, Yayasan Hayandra Peduli, Jakarta, Indonesia; Subroto, W.R., Hayandra Lab, Yayasan Hayandra Peduli, Jakarta, Indonesia; Afini, I., Hayandra Lab, Yayasan Hayandra Peduli, Jakarta, Indonesia; Widyastuti, T., Hayandra Lab, Yayasan Hayandra Peduli, Jakarta, Indonesia; Zakiyah, A., Hayandra Lab, Yayasan Hayandra Peduli, Jakarta, Indonesia; Ernanda, D., Hayandra Lab, Yayasan Hayandra Peduli, Jakarta, Indonesia; Aini, N., Hayandra Lab, Yayasan Hayandra Peduli, Jakarta, Indonesia; Jusryanti, Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Sulaeha, A.D., Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Prestiani, S.I., Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Donna, I.M., Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Habibi, Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Mutiara, M.S., Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia |
Introduction: Autologous platelet-rich plasma (PRP) has been a growing trend in the field of medicine due to its broad range of application and is considered safe from bloodborne diseases. Furthermore, various studies have tried to optimize the use of autologous PRP through various preparation protocols, including PRP activation. However, most of the studies available have not evaluated the safety for intravenous delivery of PRP, especially autologous activated PRP (aaPRP). Therefore, this study aimed to evaluate the safety of intravenous delivery of aaPRP. Methods: Blood was drawn from each patient and aaPRP was isolated through calcium activation and light irradiation. Each aaPRP was administered intravenously to all patients. Adverse events were documented and analyzed. Results: Six hundred eleven patients participated in this study with a total of 4244 aaPRP therapies. Quality control of autologous aaPRP showed no platelets present after both calcium activation and light irradiation. No adverse events such as allergic reaction, infection, and coagulation problems were observed on all patients over the course of the study. Conclusion: Our results showed that intravenous administration of autologous aaPRP is safe even in patients with various pathological conditions. © 2021. Karina Karina, et al.; licensee University of Sarajevo - Faculty of Health Studies. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Intravenous infusion; platelet-rich plasma; safety |
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University of Sarajevo - Faculty of Health Studies |
22327576 |
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Article |
Q4 |
112 |
28273 |
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749 |
Victor A.A., Yandandri V.R., Gondowiardjo T.D., Dharma R., Jusman S.W., Prihartono J. |
57191055282;57226248415;54956727400;12777817700;36518792100;6602605635; |
Effect of laser photocoagulation before vitrectomy to hypoxia inducible factor-1alfa and intracellular adhesive molecule-1 in diabetic patients: A randomized clinical trial |
2021 |
Retina-Vitreus |
30 |
2 |
|
204 |
211 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85110980758&doi=10.37845%2fret.vit.2021.30.37&partnerID=40&md5=0facab036ffe38e8aaf9f0feeac57868 |
Faculty of Medicine, Cipto Mangunkusumo National General Hospital, Ophthalmology, DKI Jakarta, Indonesia; Faculty of Medicine, Clinical Pathology, DKI Jakarta, Jakarta, Indonesia; Faculty of Medicine, Biochemistry Department, Faculty of Medicine, University of Indonesia, DKI Jakarta, Indonesia; Faculty of Medicine, Community Department, Faculty of Medicine, University of Indonesia, DKI Jakarta, Indonesia |
Victor, A.A., Faculty of Medicine, Cipto Mangunkusumo National General Hospital, Ophthalmology, DKI Jakarta, Indonesia; Yandandri, V.R., Faculty of Medicine, Cipto Mangunkusumo National General Hospital, Ophthalmology, DKI Jakarta, Indonesia; Gondowiardjo, T.D., Faculty of Medicine, Cipto Mangunkusumo National General Hospital, Ophthalmology, DKI Jakarta, Indonesia; Dharma, R., Faculty of Medicine, Clinical Pathology, DKI Jakarta, Jakarta, Indonesia; Jusman, S.W., Faculty of Medicine, Biochemistry Department, Faculty of Medicine, University of Indonesia, DKI Jakarta, Indonesia; Prihartono, J., Faculty of Medicine, Community Department, Faculty of Medicine, University of Indonesia, DKI Jakarta, Indonesia |
Purpose: The vitreous concentration of Hypoxia-inducible Factor-1α (HIF-1α) and Intercellular Adhesive Molecule-1 (ICAM-1) were related to the permeability of retinal vessels and the grades of macular edema in proliferative diabetic retinopathy (PDR). Prior studies have showed that pan-retinal photocoagulation (PRP) is benefi cial in treating PDR. The aim of this study is to determine how pre-treatment with PRP before vitrectomy affect the vitreous level of HIF-1 and ICAM-1 in patients with PDR. Materials and Methods: A randomized clinical trial study was conducted to 22 eyes in Cipto Mangunkusumo National General Hospital, Indonesia. At the beginning of PRP, just before vitrectomy, and at 2, 4, and 12 weeks after vitrectomy, central macular thickness (CMT) was measured using optical coherence tomography (OCT). Undiluted vitreous humour was extracted during vitrectomy to obtain HIF-1α and ICAM-1 concentration. Results: In the control and the photocoagulation group, the average level of HIF-1α (ng/mL) were 0.152±0.015 and 0.164±0.033 respectively. The average level of ICAM-1 (ng/mL) in control group and pre-treated group were 17,840±14,140 and 27,027±10,452 respectively. No statistically signifi cant difference was seen in the level of HIF-1α and ICAM-1 between each group. The correlation between vitreous ICAM- 1 and HbA1c was statistically signifi cant (r=0.463, p=0.03). No signifi cant differences for CMT at pre-vitrectomy, or 2 and 4 weeks after vitrectomy. Statistically signifi cant difference was observed at 12 weeks after follow-up (p=0.049). The correlation between vitreous level of HIF-Iα and CMT in the control and laser group are r = 0.447 and r = 0.32, respectively. Conclusion: Laser photocoagulation 1-2 weeks prior to vitrectomy did not lower vitreous concentration of HIF-1α and ICAM-1. © 2021 Gazi Eye Foundation. All rights reserved. |
Diabetic retinopathy; Hypoxia-Inducible Factor 1; Intercellular Adhesion Molecule-1; Light coagulation |
hypoxia inducible factor 1alpha; intercellular adhesion molecule 1; adult; Article; cataract; central macular thickness; controlled study; diabetic patient; female; follow up; glaucoma; human; hyphema; laser coagulation; major clinical study; male; middle aged; optical coherence tomography; pars plana vitrectomy; proliferative diabetic retinopathy; randomized controlled trial; retina blood vessel; retina detachment; vitrectomy; vitreous body; vitreous hemorrhage |
Gazi Eye Foundation |
13001256 |
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Article |
Q4 |
110 |
28729 |
|
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|
750 |
Amalia R., Bustamam A., Yudantha A.R., Victor A.A. |
57226194312;36815737800;55489644900;57191055282; |
Diabetic retinopathy detection and captioning based on lesion features using deep learning approach |
2021 |
Communications in Mathematical Biology and Neuroscience |
2021 |
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59 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85110864476&doi=10.28919%2fcmbn%2f5832&partnerID=40&md5=e3e95d1370fc8ff4347362007e9add2e |
Department of Mathematics, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, 16424, Indonesia; Department of Ophthalmology, Faculty of Medicine, Universitas Indonesia Cipto Mangunkusumo National General Hospital, Jakarta Pusat, 10430, Indonesia |
Amalia, R., Department of Mathematics, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, 16424, Indonesia; Bustamam, A., Department of Mathematics, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, 16424, Indonesia; Yudantha, A.R., Department of Ophthalmology, Faculty of Medicine, Universitas Indonesia Cipto Mangunkusumo National General Hospital, Jakarta Pusat, 10430, Indonesia; Victor, A.A., Department of Ophthalmology, Faculty of Medicine, Universitas Indonesia Cipto Mangunkusumo National General Hospital, Jakarta Pusat, 10430, Indonesia |
Diabetic Retinopathy (DR) can lead to vision loss if the patient does not get effective treatment based on the patient’s condition. Early detection is needed to know what an effective treatment for those patients is. For helping ophthalmologists, DR detection methods using computer-based were developed. Ophthalmologists can use the result of the method as a consideration in diagnosing the class of DR. One of the powerful methods is deep learning. The proposed method uses two deep learning architectures, namely Convolutional Neural Network (CNN) and Recurrent Neural Network (RNN), for DR detection. CNN is used to detect DR lesion features, and RNN is used for captioning based on those lesion features. We used three pre-trained CNN models, including AlexNet, VGGNet and GoogleNet, and used Long Short-Term Memory (LSTM) as RNN models. In the image preprocessing, we applied contrast enhancement using Contrast Limited Adaptive Histogram Equalization (CLAHE) and compared the results with those without CLAHE. We have done the training and testing process with a different proportion of data. The experimental results show that our proposed method can detect the lesion features and generate caption with the highest average accuracy of 96.12% for GoogleNet and LSTM with CLAHE and the proportion 70% training data 30% testing data. © 2021 the author(s). |
Convolutional neural network (CNN); Deep learning; Diabetic retinopathy; Long short-term memory (LSTM) |
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SCIK Publishing Corporation |
20522541 |
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Article |
Q4 |
189 |
20081 |
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