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74 |
Darmadi J., Batubara R.R., Himawan S., Azizah N.N., Audah H.K., Arsianti A., Kurniawaty E., Ismail I.S., Batubara I., Audah K.A. |
57222431230;57220745212;57222422725;57191927056;57222432027;36124567000;57214889313;7103005751;26031903000;15119028200; |
Evaluation of Indonesian mangrove Xylocarpus granatum leaves ethyl acetate extract as potential anticancer drug |
2021 |
Scientific Reports |
11 |
1 |
6080 |
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2 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102715983&doi=10.1038%2fs41598-021-85383-3&partnerID=40&md5=0ea6644166c67e4defd273cae7f6aa43 |
Department of Biomedical Engineering, Swiss German University, Tangerang, 15143, Indonesia; Drug Development Research Center, IMERI, University of Indonesia, Jakarta, 10430, Indonesia; Department of Medical Chemistry, University of Indonesia, Jakarta, 10430, Indonesia; Faculty of Medicine, University of Lampung, Bandar Lampung, 35145, Indonesia; Institute of Bioscience, Universiti Putra Malaysia, Serdang, 43400, Malaysia; Biopharmaca Tropica Research Center, IPB University, Bogor, 16680, Indonesia; Department of Chemistry, IPB University, Bogor, 16680, Indonesia; Directorate of Academic Research and Community Service, Swiss German University, Tangerang, 15143, Indonesia |
Darmadi, J., Department of Biomedical Engineering, Swiss German University, Tangerang, 15143, Indonesia; Batubara, R.R., Department of Biomedical Engineering, Swiss German University, Tangerang, 15143, Indonesia; Himawan, S., Department of Biomedical Engineering, Swiss German University, Tangerang, 15143, Indonesia; Azizah, N.N., Drug Development Research Center, IMERI, University of Indonesia, Jakarta, 10430, Indonesia; Audah, H.K., Department of Biomedical Engineering, Swiss German University, Tangerang, 15143, Indonesia; Arsianti, A., Drug Development Research Center, IMERI, University of Indonesia, Jakarta, 10430, Indonesia, Department of Medical Chemistry, University of Indonesia, Jakarta, 10430, Indonesia; Kurniawaty, E., Faculty of Medicine, University of Lampung, Bandar Lampung, 35145, Indonesia; Ismail, I.S., Institute of Bioscience, Universiti Putra Malaysia, Serdang, 43400, Malaysia; Batubara, I., Biopharmaca Tropica Research Center, IPB University, Bogor, 16680, Indonesia, Department of Chemistry, IPB University, Bogor, 16680, Indonesia; Audah, K.A., Department of Biomedical Engineering, Swiss German University, Tangerang, 15143, Indonesia, Directorate of Academic Research and Community Service, Swiss German University, Tangerang, 15143, Indonesia |
Local Xylocarpus granatum leaves were extracted by ethyl acetate solvent and characterized by TLC fingerprinting and 2D 1H NMR spectroscopy to contain phenolic compounds as well as several organic and amino acids as metabolic byproducts, such as succinic acid and acetic acid. Traces of flavonoids and other non-categorized phenolic compounds exhibited intermediate antioxidant activity (antioxidant IC50 84.93 ppm) as well as anticancer activity against HeLa, T47D, and HT-29 cell lines; which the latter being most effective against HT-29 with Fraction 5 contained the strongest activity (anticancer IC50 23.12 ppm). Extracts also behaved as a natural growth factor and nonlethal towards brine shrimps as well as human adipose-derived stem cell hADSC due to antioxidative properties. A stability test was performed to examine how storage conditions factored in bioactivity and phytochemical structure. Extracts were compared with several studies about X. granatum leaves extracts to evaluate how ethnogeography and ecosystem factored on biologically active compounds. Further research on anticancer or antioxidant mechanism on cancer cells is needed to determine whether the extract is suitable as a candidate for an anticancer drug. © 2021, The Author(s). |
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acetic acid; antineoplastic agent; plant extract; adipose tissue; animal; Artemia; chemistry; drug screening; HeLa cell line; HT-29 cell line; human; MCF-7 cell line; Meliaceae; metabolism; neoplasm; plant leaf; stem cell; Acetates; Adipose Tissue; Animals; Antineoplastic Agents, Phytogenic; Artemia; Drug Screening Assays, Antitumor; HeLa Cells; HT29 Cells; Humans; MCF-7 Cells; Meliaceae; Neoplasms; Plant Extracts; Plant Leaves; Stem Cells |
Nature Research |
20452322 |
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33727582 |
Article |
Q1 |
1240 |
3130 |
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394 |
Abdullah M., Sudrajat D.G., Muzellina V.N., Kurniawan J., Rizka A., Utari A.P., Pribadi R.R., Idrus M.F., Yusra Y., Meilany S., Surandy A., Shatri H., Rinaldi I., Pitoyo C.W., Renaldi K. |
7103393434;54893136100;57223968527;57193251655;57203243658;57113585900;57194732282;57223976376;57220998367;57193709281;57223964417;28767986500;23475122400;26022606900;57190963547; |
The value of anal swab RT-PCR for COVID-19 diagnosis in adult Indonesian patients |
2021 |
BMJ Open Gastroenterology |
8 |
1 |
e000590 |
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3 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106611204&doi=10.1136%2fbmjgast-2020-000590&partnerID=40&md5=cc17ce689ac73f1e46021151c9319871 |
Gastroenterology, Pancreatobilliary, and Endoscopy Division, Internal Medicine Department, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Indonesia Medical Education and Research Institute - Human Cancer Research Center, University of Indonesia Faculty of Medicine, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Hepatobilliary Division, Internal Medicine Department, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Geriatric Division, Internal Medicine Department, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Department of Clinical Pathology, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region Jakarta, Indonesia; Research Center for Virology and Cancer Pathobiology, University of Indonesia Faculty of Medicine, Jakarta, Special Capital Region of Jakarta, Indonesia; Psychosomatic and Palliative Care Division, Internal Medicine Department, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Special Capital Region of Jakarta, Central Jakarta, Indonesia; Hematology and Medical Oncology Division, Internal Medicine Department, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Respirology and Critical Care Division, Internal Medicine Department, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia |
Abdullah, M., Gastroenterology, Pancreatobilliary, and Endoscopy Division, Internal Medicine Department, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia, Indonesia Medical Education and Research Institute - Human Cancer Research Center, University of Indonesia Faculty of Medicine, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Sudrajat, D.G., Gastroenterology, Pancreatobilliary, and Endoscopy Division, Internal Medicine Department, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Muzellina, V.N., Gastroenterology, Pancreatobilliary, and Endoscopy Division, Internal Medicine Department, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Kurniawan, J., Hepatobilliary Division, Internal Medicine Department, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Rizka, A., Geriatric Division, Internal Medicine Department, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Utari, A.P., Gastroenterology, Pancreatobilliary, and Endoscopy Division, Internal Medicine Department, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Pribadi, R.R., Gastroenterology, Pancreatobilliary, and Endoscopy Division, Internal Medicine Department, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Idrus, M.F., Gastroenterology, Pancreatobilliary, and Endoscopy Division, Internal Medicine Department, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Yusra, Y., Department of Clinical Pathology, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region Jakarta, Indonesia; Meilany, S., Research Center for Virology and Cancer Pathobiology, University of Indonesia Faculty of Medicine, Jakarta, Special Capital Region of Jakarta, Indonesia; Surandy, A., Gastroenterology, Pancreatobilliary, and Endoscopy Division, Internal Medicine Department, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Shatri, H., Psychosomatic and Palliative Care Division, Internal Medicine Department, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Special Capital Region of Jakarta, Central Jakarta, Indonesia; Rinaldi, I., Hematology and Medical Oncology Division, Internal Medicine Department, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Pitoyo, C.W., Respirology and Critical Care Division, Internal Medicine Department, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia; Renaldi, K., Gastroenterology, Pancreatobilliary, and Endoscopy Division, Internal Medicine Department, Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo, Central Jakarta, Special Capital Region of Jakarta, Indonesia |
Objective This study will test the performance of the anal swab PCR test when compared with the nasopharyngeal swab PCR test as a diagnostic tool for COVID-19. Design An observational descriptive study which included hospitalised suspected, or probable cases of hopitalised COVID-19 patients, conducted in Dr. Cipto Mangunkusumo National Hospital, Ciputra Hospital, Mitra Keluarga Depok Hospital and Mitra Keluarga Kelapa Gading Hospital, Indonesia. Epidemiological, clinical, laboratory and radiology data were obtained. Nasopharyngeal and anal swabs specimens were collected for SARS-CoV-2 RNA detection. Results We analysed 136 subjects as part of this study. The clinical spectrum of COVID-19 manifesation in this study was typical of hospitalised patients, with 25% classified as mild cases, 14.7% in severe condition and 12.5% of subjects classified as having acute respiratory distress syndrome. When compared with nasopharyngeal swab as the standard specimen for reverse transcription polymerase chain reaction (RT-PCR) detection of SARS-CoV-2 antigen, the sensitivity and specificity of the anal swab was 36.7% and 93.8%, respectively. The positive and negative predictive value were 97.8% and 16.5 %, respectively. The performance of the anal swab remained similar when only the subgroup of patients with gastrointestinal symptoms (n=92, 67.6%) was analysed (sensitivity 40% and specificity 91.7%). Out of all the subjects included in analysis, 67.6% had gastrointestinal symptoms. Similarly, 73.3% of patients in the anal swab-positive group had gastrointestinal symptoms. The two most common gastrointestinal symptoms in the subjects' population were nausea and anorexia. Conclusion Anal swab specimen has low sensitivity (36.7%) but high specificity (93.8%) for detecting SARS-CoV-2 antigen by RT-PCR. Only one additional positive result was found by anal swab among the nasopharyngeal swab-negative group. Anal swab may not be needed as an additional test at the beginning of a patient's diagnostic investigation and nasopharyngeal swab RT-PCR remains as the standard diagnostic test for COVID-19. © 2020 American Society of Mechanical Engineers (ASME). All rights reserved. |
anal; COVID-19; diagnostic virology |
abdominal pain; adult; adult respiratory distress syndrome; anal swab; anorexia; Article; body mass; clinical laboratory; controlled study; coronavirus disease 2019; coughing; COVID-19 testing; current smoker; diagnostic test accuracy study; diarrhea; ex-smoker; female; fever; gastrointestinal symptom; headache; heart disease; hospital patient; human; hypertension; Indonesian; lung disease; major clinical study; malaise; male; mortality rate; nasopharyngeal swab; nausea; non insulin dependent diabetes mellitus; practice guideline; predictive value; priority journal; respiratory tract infection; reverse transcription polymerase chain reaction; sensitivity and specificity; sore throat |
BMJ Publishing Group |
20544774 |
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Article |
Q1 |
1240 |
3126 |
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