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Kusmardi K., Situmorang N.Y., Zuraidah E., Estuningtyas A., Tedjo A. |
56966625300;57357530500;57357911500;55650360200;57189320451; |
The effect of mahkota dewa (Phaleria macrocarpa) leaf extract on the Mucin 1 expression in mice colonic epithelial cells induced by dextran sodium sulfate (DSS) |
2021 |
Pharmacognosy Journal |
13 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120333886&doi=10.5530%2fPJ.2021.13.181&partnerID=40&md5=668714fd6caa4dce8362e17d7e54ca12 |
Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Drug Development Research Center, Indonesia Medical Education and Resesarch Institute (IMERI), Universitas Indonesia, Jakarta, Indonesia; Human Cancer Research Center, IMERI, Universitas Indonesia, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Kusmardi, K., Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Drug Development Research Center, Indonesia Medical Education and Resesarch Institute (IMERI), Universitas Indonesia, Jakarta, Indonesia, Human Cancer Research Center, IMERI, Universitas Indonesia, Jakarta, Indonesia; Situmorang, N.Y., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Zuraidah, E., Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Estuningtyas, A., Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Tedjo, A., Drug Development Research Center, Indonesia Medical Education and Resesarch Institute (IMERI), Universitas Indonesia, Jakarta, Indonesia, Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Background: Inflammatory bowel disease is a chronic inflammation caused by the malignant inflammation response and if not treated, could lead to colorectal cancer. One of the researched treatment is mahkota dewa (Phaleria macrocarpa) leaf extract that has flavonoid compound known to reduce inflammation. This study was aimed to prove that mahkota dewa leaf extract could reduce inflammation of mice colon induced with dextran sodium sulfate (DSS) and observe MUC1 expression from colon epithelial crypt of Lieberkuhn. Methods: This was a laboratory experiment using biological material (paraffin block) taken from 28 mice and divided into 5 groups: normal, aspirin, low and high dose mahkota dewa, and negative control. They were processed into immunohistochemistry and stained microscopic slides. Afterwards, they were observed with 400x magnification and 5 field-of-view of mice colon crypt of lieberkuhn. Then MUC1 expression was counted using ImageJ to obtain mean immunohistochemistry score and analyzed with SPSS. Results: There were significant reduction of MUC1 expressions from normal, aspirin, and high dose mahkota dewa groups compared to the negative control group. The result shown MUC1 expression from high dose mahkota dewa (M=149.90,SD=3.81) and aspirin (M=158.92,SD=5.28) were closer to normal group (M=148.02,SD=5.28). There were no significant results between negative (M=175.39,SD=14.30) and low dose mahkota dewa group (M=149.90,SD=5.02). Conclusion: There was a reduction of MUC1 expression in DSS-induced mice colonic epithelial cells for high dose mahkota dewa group. This shown that high dosage mahkota dewa leaf extract could reduce inflammation like aspirin. © 2021 Phcogj.Com. |
Anti-inflammatory agent; Colon epithelial cell; Inflammatory bowel disease; Mahkota dewa (Phaleria macrocarpa); MUC 1 expression |
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Rusdi N.K., Yuliana W.L., Purwaningsih E.H., Hestiantoro A., Kusmardi K. |
57211475250;57358166800;57186723500;8743255100;56966625300; |
Subchronic toxicity of lunasin targeted extract (ET-Lun) from soybean seed (Glycine max (L.) Merr.): Perspective from liver histopathology, SGOT, and SGPT levels in Sprague Dawley rats |
2021 |
Pharmacognosy Journal |
13 |
6 |
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1384 |
1388 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120333876&doi=10.5530%2fPJ.2021.13.175&partnerID=40&md5=05cdb4c0d35834a5b3b9ce79bf4c1e56 |
Doctoral Program for Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. Hamka, Jakarta, Indonesia; Department of Pharmacy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Drug Development Research Cluster, Indonesian Medical Education and Reseach Institute, Universitas INDONESIA, Indonesia; Department Obstetrics and Gynaecology, School of Medicine, Universitas Indonesia, Dr Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Human Cancer Research Cluster, Indonesian Medical Education and Reseach Institute, Universitas INDONESIA, Indonesia |
Rusdi, N.K., Doctoral Program for Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. Hamka, Jakarta, Indonesia; Yuliana, W.L., Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. Hamka, Jakarta, Indonesia; Purwaningsih, E.H., Department of Pharmacy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Drug Development Research Cluster, Indonesian Medical Education and Reseach Institute, Universitas INDONESIA, Indonesia; Hestiantoro, A., Department Obstetrics and Gynaecology, School of Medicine, Universitas Indonesia, Dr Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Kusmardi, K., Doctoral Program for Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Drug Development Research Cluster, Indonesian Medical Education and Reseach Institute, Universitas INDONESIA, Indonesia, Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Human Cancer Research Cluster, Indonesian Medical Education and Reseach Institute, Universitas INDONESIA, Indonesia |
Background: Lunasin Targeted Extract (ET-Lun) has a pharmacology effect in inhibiting inflammation by decreasing COX-2 and iNOS expression. ET-Lun could increase apoptosis and decrease dysplasia (p > 0,05). In addition, ET-Lun could decrease EGFR expression in breast cancer rats. The acute toxicity showed ET-Lun has LD50 more than 5000 mg/kg BW and was practically non-toxic. Objective: this study aimed to determine the subchronic toxicity of ET-Lun. Methods: Male and female Sprague Dawley rats (n=40) were divided into 4 groups, the control group and treatment group ET-Lun dose of 250 mg/Kg BW, 500 mg/kg BW, and 750 mg/kg BW. The ET-Lun was administered for 90 days. On the 91st day, the animals were dissected and examined for SGOT-SGPT levels, liver histopathology, and diameter of the central vein. Results: The SGOT-SGPT levels showed no significant difference between the treatment group and the control group (p > 0.05). On microscopic observation, there was no change or damage to the liver of rats in each group. The diameter of the central vein of the rat liver shows no significant difference between the control and treatment groups. Conclusion: The ET-Lun does not produce adverse effects in liver rats after subchronic treatment. © 2021 Phcogj.Com. |
Liver; Lunasin; SGOT; SGPT; Soybean; Subchronic toxicity |
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Kusmardi K., Hairi B.N., Lubis N.S., Lestari T.W., Intan P.R. |
56966625300;57357912100;57222661567;57208401033;57357912200; |
The effect of sambiloto and spirulina combination on Mucin-1 protein expression in medial colon of Plasmodium berghei ANKA infected mice |
2021 |
Pharmacognosy Journal |
13 |
6 |
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1359 |
1365 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120320780&doi=10.5530%2fPJ.2021.13.172&partnerID=40&md5=1af1902c44df820801a8286e3e589f81 |
Anatomical Pathology Department, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Drug Development Research Cluster, Indonesia Medical Educational and Research Institute, Jakarta, Indonesia; Human Cancer Research Cluster, Indonesia Medical Educational and Research Institute, Jakarta, Indonesia; Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Undergraduate Program, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Parasitology Department, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; National Institute of Health Research and Development, Ministry of Health of Indonesia, Jakarta, Indonesia; Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia |
Kusmardi, K., Anatomical Pathology Department, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Drug Development Research Cluster, Indonesia Medical Educational and Research Institute, Jakarta, Indonesia, Human Cancer Research Cluster, Indonesia Medical Educational and Research Institute, Jakarta, Indonesia, Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Hairi, B.N., Undergraduate Program, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Lubis, N.S., Parasitology Department, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Lestari, T.W., National Institute of Health Research and Development, Ministry of Health of Indonesia, Jakarta, Indonesia; Intan, P.R., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia |
Malaria still be health problem in the world, especially in Eastern Indonesia. Malaria’s inflammation and metabolism defect can cause colonic damage, such as enhancement Muc-1 protein expression and goblet cells hyperplasia. Sambiloto and spirulina combination as antiinflammatory and antioxidative agent can prevent medial colon damage Plasmodium berghei ANKA infected mice. The aim of the study to show the effect of sambiloto and spirulina combination on Muc-1 protein activity in medial colon Plasmodium berghei ANKA infected mice. This study use preserve male Swiss Webser mice colonic tissue which has inoculated by Plasmodium berghei ANKA, whose treatment group include positive control (dehyroartemisin piperaquine), negative control (carboxymethil cellulose), AP (sambiloto), AP+ES (sambiloto+spirulina extract), and AP+PS (sambiloto+spirulina powder) and terminated after 28 days of treatment. Colonic tissue was stained with immunohistochemistry and observed using light microscope (400x) in five different field and was analyzed with ImageJ® sowtware, and statisitcal analysis was done with SPSS 20.0. According to One Way ANOVA and Duncan posthoc test, only AP+PS(120,98 ±3,37), which significantly difference between negative control, AP, and AP+ES group. Meanwhile, between DHP, AP+PS group not significantly differenece. Sambiloto extract and spirulina powder combination can reduce Muc-1 protein expression in medial colon Plasmodium berghei ANKA infected mice. © 2021 Phcogj.Com. |
Medial colon; Muc-1; Plasmodium berghei ANKA; Sambiloto; Spirulina |
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Rusdi N.K., Purwaningsih E.H., Hestiantoro A., Elya B., Kusmardi K. |
57211475250;57186723500;8743255100;14014224500;56966625300; |
In vivo antimammary tumor effects of soybean extract with targeted lunasin (ET-Lun) |
2021 |
Pharmacognosy Journal |
13 |
5 |
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1269 |
1276 |
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2 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115297490&doi=10.5530%2fpj.2021.13.160&partnerID=40&md5=d96a1538654afeda0377ba6b0d8a5e38 |
Doctoral Program for Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. Hamka, Jakarta, Indonesia; Department of Pharmacy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department Obstetrics and Gynaecology, School of Medicine, Universitas Indonesia, Dr Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Department of Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia; Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Drug Development Research Cluster, Indonesian Medical Education and Reseach Institute, Universitas INDONESIA, Indonesia; Human Cancer Research Cluster, Indonesian Medical Education and Research Institute, Universitas INDONESIA, Indonesia |
Rusdi, N.K., Doctoral Program for Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. Hamka, Jakarta, Indonesia; Purwaningsih, E.H., Department of Pharmacy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Drug Development Research Cluster, Indonesian Medical Education and Reseach Institute, Universitas INDONESIA, Indonesia; Hestiantoro, A., Department Obstetrics and Gynaecology, School of Medicine, Universitas Indonesia, Dr Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Elya, B., Department of Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia; Kusmardi, K., Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Drug Development Research Cluster, Indonesian Medical Education and Reseach Institute, Universitas INDONESIA, Indonesia, Human Cancer Research Cluster, Indonesian Medical Education and Research Institute, Universitas INDONESIA, Indonesia |
Background/Objective: Lunasin is a peptide, consist of 44 amino acids which have anti-cancer, antioxidant, and anti-inflammatory activity. The price of commercial Lunasin was very expensive due to the high cost of lunasin synthesis and the lack of methods to obtain the pure lunasin weight from plant sources, involving time-consuming analytical instruments. To overcome these problems, the soybean extract with targeted Lunasin (ET-Lun) was made. The aim of this study was to investigate anti-cancer properties of ET-Lun in breast cancer models in vivo. Methods: Effect of ET-Lun was evaluated in 7,12-Dimetilbenz[a]antrasen (DMBA) induced breast cancer rat model. Tumor Mass, volume, and number were measured. The expression of HER2 and EGFR from each treatment group in DMBA-induced rat was evaluated using immunohistochemistry. Results: The results shown that ET-Lun could reduced tumor volume (p=0,021). ET-Lun decreased EGFR expression compared to negative control DMBA (p=0,012). Conclusions: These results indicated that the ET-Lun has anti-breast cancer activity in vivo. © 2021 Phcogj.Com. |
Breast cancer; EGFR; HER2; In-vivo; Soybean |
dimethylbenz[a]anthracene; epidermal growth factor receptor; epidermal growth factor receptor 2; lunasin; peptide; soybean extract; tamoxifen; unclassified drug; aged; animal experiment; animal model; animal tissue; antineoplastic activity; Article; breast cancer; controlled study; female; immunohistochemistry; in vivo study; nonhuman; protein expression; rat; soybean; tumor number; tumor volume |
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Firdaus M.D., Artanti N., Hanafi M., Rosmalena |
57226243708;14832374300;26644895300;56891769500; |
Phytochemical constituents, and in vitro antidiabetic and antioxidant properties of various extracts of kenikir (cosmos caudatus) leaves |
2021 |
Pharmacognosy Journal |
13 |
4 |
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890 |
895 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85110937044&doi=10.5530%2fpj.2021.13.114&partnerID=40&md5=90452554ceab08be4e58b8af98f14dc7 |
Department of Medicinal Chemistry, Faculty of Medicine, Universitas Indonesia, Depok, 16424, Indonesia; Research Center for Chemistry, Indonesian Institute of Sciences, Kawasan PUSPITEK, Serpong, Banten, South Tangerang, Indonesia |
Firdaus, M.D., Department of Medicinal Chemistry, Faculty of Medicine, Universitas Indonesia, Depok, 16424, Indonesia; Artanti, N., Research Center for Chemistry, Indonesian Institute of Sciences, Kawasan PUSPITEK, Serpong, Banten, South Tangerang, Indonesia; Hanafi, M., Research Center for Chemistry, Indonesian Institute of Sciences, Kawasan PUSPITEK, Serpong, Banten, South Tangerang, Indonesia; Rosmalena, Department of Medicinal Chemistry, Faculty of Medicine, Universitas Indonesia, Depok, 16424, Indonesia |
Type 2 diabetes mellitus (T2DM) is one of the most common degenerative disorders. For therapeutic use, herbs are commonly used in Indonesia for T2DM treatment, one of them is (Cosmos caudatus) kenikir's leaves. In previous studies, kenikir's leaves have high antidiabetic and antioxidant activity. However, a comparison of antidiabetic activity from many extracts of kenikir's leave is remain unclear. This study will compare the antidiabetic and antioxidant properties of various kenikir's leave extract. Kenikir's leaves are extracted by maceration methods for three days using three different solvents: boiling water, 50% ethanol, dan ethanol 100%.Then, phenolic and flavonoid content will be measured, as well as antioxidant properties by DPPH radical scavenging activity assay, and antidiabetic properties by α-glucosidase inhibition assay, also LCMS/MS will be used to predict the compound from each extract. The result shows that 50% ethanol extract has highest phenolic and flavonoid content than others. It also has significantly higher antioxidant (p<0.05) and antidiabetic (p<0.05) properties than others. Meanwhile, LCMS/MS result of 50% ethanol extract predicts 6 chemical component, that quercetin is the most dominant compound. 50% ethanol extract of kenikir's leaves is superior from other extracts on phenolic and flavonoid content, antioxidant properties, and antidiabetic properties. © 2021 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
α-Glucosidase |
1,1 diphenyl 2 picrylhydrazyl; 2 beta 3 beta dihydroxy nortropane; alcohol; alpha glucosidase; antidiabetic agent; antioxidant; Cosmos caudatus extract; delta humulene; digiprolactone; flavonoid; genistin; gentiatibetine; glucopyranoside; herbaceous agent; oroxin B; phenol; phenylproprionic acid; phytochemical; plant extract; quercetin; solvent; spathulenol; stearidonic acid; unclassified drug; valine; water; antidiabetic activity; antioxidant activity; Article; boiling point; controlled study; Cosmos (genus); DPPH radical scavenging assay; human; in vitro study; Kenikir leaf; liquid chromatography-mass spectrometry; nonhuman; plant leaf |
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Rosmalena, Widyastuti P.A., Yazid F., Ambarwati N.S.S., Ahmad I. |
56891769500;57226249984;57207890516;57193830343;57190669391; |
Phytochemicals and antioxidant activities evaluation of origanum vulgare (L.) stem bark extracts |
2021 |
Pharmacognosy Journal |
13 |
4 |
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965 |
970 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85110932652&doi=10.5530%2fpj.2021.13.124&partnerID=40&md5=01f50c35c8a94d648081fcd99e50d8fa |
Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, South Jakarta, Jakarta, 10430, Indonesia; Medical Student, Faculty of Medicine, Universitas Indonesia, South Jakarta, Jakarta, 10430, Indonesia; Department of Cosmetology, Faculty of Engineering, Universitas Negeri Jakarta, East Jakarta, Jakarta, 13220, Indonesia; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Mulawarman, East Kalimantan, Samarinda, 75119, Indonesia |
Rosmalena, Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, South Jakarta, Jakarta, 10430, Indonesia; Widyastuti, P.A., Medical Student, Faculty of Medicine, Universitas Indonesia, South Jakarta, Jakarta, 10430, Indonesia; Yazid, F., Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, South Jakarta, Jakarta, 10430, Indonesia; Ambarwati, N.S.S., Department of Cosmetology, Faculty of Engineering, Universitas Negeri Jakarta, East Jakarta, Jakarta, 13220, Indonesia; Ahmad, I., Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Mulawarman, East Kalimantan, Samarinda, 75119, Indonesia |
The present study aimed to evaluate phytochemical and antioxidant activity (in vitro and in vivo) of Origanum vulgare (L.) ethanolic extract. The phytochemical test was assessed using the Clule method in ethanol, ethyl acetate, and hexane. In vitro evaluation of antioxidant activity was determined by radical scavenging assay using DPPH (2,2-diphenyl-1-picrylhydrazyl) as an artificial free radical activity. In vivo test was conducted to evaluate the effect of malondialdehyde (MDA) level in blood plasma during maximum physical activity treatment. In vivo test was done using 25 male Sprague Dawley rats in pre and post-test control group design. The phytochemical test of O. vulgare ethanol extract was showed some compounds, such as a flavonoid, alkaloid, triterpenoid/steroid, essential oil, and tannin, then in ethyl acetate and hexane. In vitro assay showed that O. vulgare extract has strong antioxidant activity with an IC50 value of 133.47 µg/mL. While in the in vivo test, the most effective dosage is 20 mg/200 gr B.W., represented by a significant decrease of MDA level (0.509 nmol/mL) before and after treatment. So, the ethanolic extract of clove has potency as an herbal antioxidant because of the low level of IC50 and can decrease the MDA level. © 2021 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
2; 2-diphenyl-1-picrylhydrazyl; Antioxidant activity; Malondialdehyde; Origanum vulgare (L.); Phytochemical |
1,1 diphenyl 2 picrylhydrazyl; acetic acid ethyl ester; alcohol; alkaloid; antioxidant; ascorbic acid; essential oil; flavonoid; free radical; hexane; malonaldehyde; Origanum vulgare ethanolic extract; phytochemical; plant extract; saponin; steroid; tannin; triterpenoid; unclassified drug; animal experiment; animal tissue; antioxidant activity; Article; bark; blood sampling; clove; controlled study; DPPH radical scavenging assay; drug potency; in vitro study; in vivo study; male; nonhuman; physical activity; pretest posttest design; rat; Sprague Dawley rat |
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Kusmardi K., Wiyarta E., Estuningtyas A., Sahar N., Midoen Y.H., Tedjo A., Pakpahan A. |
56966625300;57221521342;55650360200;57212464367;57197805109;57189320451;57200109636; |
Potential inhibition by Phaleria macrocarpa leaves ethanol extract on Ki-67 expression in distal colon mouse |
2021 |
Pharmacognosy Journal |
13 |
2 |
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443 |
449 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103626996&doi=10.5530%2fpj.2021.13.56&partnerID=40&md5=0fdcd0a987696734078bf217c05eefb5 |
Department of Anatomic Pathology, Drug Development Research Cluster, Human Cancer Research Center, IMERI, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Department of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jl. Kyai Tapa, Jakarta, Indonesia |
Kusmardi, K., Department of Anatomic Pathology, Drug Development Research Cluster, Human Cancer Research Center, IMERI, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Wiyarta, E., Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Estuningtyas, A., Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Sahar, N., Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Midoen, Y.H., Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Tedjo, A., Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Pakpahan, A., Department of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jl. Kyai Tapa, Jakarta, Indonesia |
Ulcerative colitis (UC) has been an important aspect of an incurable chronic inflammatory disease over the last few decades. To find useful therapies for UC, one of which is herbal therapy, many researches have been conducted. Due to its anti-inflammatory effects, Phaleria macrocarpa (PM), an Indonesian indigenous herb, is considered to be the alternative therapy for UC. Phaleria macrocarpa Leaves Ethanol Extract (PMLEE) is then used in this research to determine its effect on UC by using Ki-67 as a marker of proliferation. PMLEE was created from dry PM content undergoing maceration. The animals were classified into six categories: normal, positive control, negative control and PMLEE group (100, 200, 300 mg/kgBW). PMLEE was then injected for 7 consecutive days into BALB/c mice that were caused by dextran sodium sulphate (DSS). DSS is used for modeling UC in the colon tissue of mice. All mice were terminated and then stained with anti-Ki-67 after their colons were extracted. Subsequently, the stained parts were analyzed with ImageJ based on the color intensity produced by the results of H-score. Based on H-score, PMLEE 300mg and 200mg has significantly decreased the expression of Ki-67 compare to the negative control (p=0.001 and p=0.01). PMLEE also has a tendency to be dose dependent based on the significant difference from PMLEE 300mg and 100mg (p=0.002). It then concludes that PMLEE is related to Ki-67 expression in cells, as it was inversely proportional in this analysis. © 2021 Phcogj.Com. |
Dextran sodium sulphate; Inflamation; Ki-67; Mahkota Dewa (Phaleria macrocarpa) |
acetylsalicylic acid; alcohol; antiinflammatory agent; dextran sulfate; Ki 67 antigen; Phaleria macrocarpa extract; plant extract; unclassified drug; animal experiment; animal model; animal tissue; antigen expression; antiinflammatory activity; Article; colon tissue; controlled study; data analysis software; descending colon; dysplasia; medicinal plant; mouse; nonhuman; Phaleria macrocarpa; plant leaf; proliferation index; scoring system; treatment duration; ulcerative colitis |
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Bahtiar A., Miranda A.J., Arsianti A. |
35365874400;57221531432;36124567000; |
The effect of artocarpus altilis (parkinson) fosberg extract supplementation on kidney ischemia-reperfusion injury rat |
2021 |
Pharmacognosy Journal |
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154 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099365078&doi=10.5530%2fpj.2021.13.21&partnerID=40&md5=13b866767b1597a22566e8ac646a0b9f |
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, West Java, 16424, Indonesia; Department of Medicinal Chemistry, Faculty of Medicine, Universitas Indonesia, Kampus UI Salemba, Jakarta, Indonesia |
Bahtiar, A., Department of Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, West Java, 16424, Indonesia; Miranda, A.J., Department of Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, West Java, 16424, Indonesia; Arsianti, A., Department of Medicinal Chemistry, Faculty of Medicine, Universitas Indonesia, Kampus UI Salemba, Jakarta, Indonesia |
Background: Acute kidney injury (AKI) is a kidney disease resulting in high morbidity and mortality levels in humans. One of the disorders classified as AKI is ischemia-reperfusion injury (IRI), characterized by two phases. The first phase is Ischemia in the kidneys due to obstruction of the renal arteries or veins, followed by the second phase, which is the occurrence of reperfusion with blood flowing back in the renal arteries veins. The aim of this current research is to analyze the efficacy of Artocarpus altilis on Kidney ischemia-reperfusion model rats. Methods: To this end, first, we established Ischaemia-reperfusion kidney injury rat. We then evaluated the Artocarpus altilis extract on IRI model rats. A total of 36 rats have grouped into six groups. Group I is the Sham group, Group II is the negative control group, Group III is the positive control group (vitamin C 100 mg/kg BW), Group IV is Dose I of Artocarpus altilis extract 50 mg/kg BW), Group V is Dose II Artocarpus altilis extract 100 mg/kg BW), Group VI is Dose III Artocarpus altilis extract 200 mg/kg BW). The vitamin C and Artocarpus altilis extract administered 14 days before and after Ischemia-reperfusion treatment. At day 0, Ischemia was made by bilateral renal pedicle clamping method for 30 minutes, sacrificed 14 days after reperfusion. The blood and histology samples were collected on day 0, a day after reperfusion, at 24 hrs after reperfusion, at 48 hrs after reperfusion, and 14 days after treatment. Results: The clamping duration of 30 minutes leads to achieving the most representative clinical IRI conditions. It shows the most significant recovery of injury conditions within the 14-day reperfusion period in IRI animal models, making it ideal for IRI operations for the preliminary test. The administration of 100 mg/kg BW of Artocarpus altilis extract could reduce the malondialdehyde plasma compared with the sham group. The SOD and Catalase activity showed improvement after reperfusion. Conclusion: Artocarpus altilis extracts showed antioxidant activity to prevent the kidney from ischemia-reperfusion injury by modulated SOD and Catalase. © 2021 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
Acute kidney disease; Artocarpus altilis (Parkinson) Fosberg; Free radicals; Reperfusion injury; Sukun |
antiinflammatory agent; antioxidant; Artocarpus altilis extract; ascorbic acid; catalase; creatinine; malonaldehyde; nitrogen; plant extract; protective agent; superoxide dismutase; unclassified drug; urea; animal experiment; animal model; animal tissue; antiinflammatory activity; antioxidant activity; Article; blood sampling; controlled study; creatinine blood level; dose response; drug efficacy; enzyme activity; enzyme blood level; glomerulus filtration rate; histopathology; male; nonhuman; rat; renal ischemia reperfusion injury; renal protection; supplementation; treatment duration; urea nitrogen blood level |
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Kusmardi K., Wiyarta E., Estuningtyas A., Sahar N., Midoen Y.H., Tedjo A. |
56966625300;57221521342;55650360200;57212464367;57197805109;57189320451; |
Potential of phaleria macrocarpa leaves ethanol extract to upregulate the expression of caspase-3 in mouse distal colon after dextran sodium sulphate induction |
2021 |
Pharmacognosy Journal |
13 |
1 |
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23 |
29 |
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2 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099343021&doi=10.5530%2fpj.2021.13.4&partnerID=40&md5=8dd1b8ac126d5a96f41668c2f3066c1f |
Department of Anatomic Pathology, Drug Development Research Cluster, Human Cancer Research Center, IMERI, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Department Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia |
Kusmardi, K., Department of Anatomic Pathology, Drug Development Research Cluster, Human Cancer Research Center, IMERI, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Wiyarta, E., Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Estuningtyas, A., Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Sahar, N., Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Midoen, Y.H., Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia; Tedjo, A., Department Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia |
Ulcerative colitis (UC) is a part of incurable chronic inflammatory disease that has gained importance over the past few decades. A lot of research has been done to find effective treatments for UC, one of which is herbal medicine. Phaleria macrocarpa (PM), an Indonesian native plant, is thought to be an alternative therapy for UC because of its anti-inflammatory properties. Therefore, in this research, Phaleria macrocarpa Leaves Ethanol Extract (PMLEE) is used to assess its effect on UC by using Caspase-3 as apoptosis marker. PMLEE was made from dried material of PM that undergo maceration. Animals were separated into six groups: normal, negative control, positive control, and PMLEE groups (100, 200, 300 mg/kgBW). PMLEE was then injected to BALB/c mice that have been induced by dextran sodium sulphate (DSS) for 7 consecutive days. DSS is used to model UC in mice colon tissue. All animals were sacrificed and their colons were collected then stained with anti-Caspase-3. The stained sections were subsequently examined with ImageJ based on color intensity which generated H-Score as the results. Based on H-Score of each group, PMLEE 300mg has significantly upregulate the expression of Caspase-3 compare to the negative control (p=0.015). PMLEE also has a tendency to be dose dependent based on the significant difference between PMLEE doses. Therefore, it concludes that PMLEE is able to upregulate the expression of Caspase-3 in colon cells as in this study it was directly proportional. © 2021 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
Apoptosis; Inflammation; Mahkota Dewa; Ulcerative colitis |
acetylsalicylic acid; alcohol; antiinflammatory agent; apoptotic protease activating factor 1; caspase 3; cytochrome c; dextran sulfate; Phaleria macrocarpa extract; plant extract; unclassified drug; animal cell; animal experiment; animal model; animal tissue; antiinflammatory activity; apoptosis; Article; cell death; cell proliferation; colon tissue; controlled study; descending colon; dysplasia; medicinal plant; mitochondrion; mouse; nonhuman; Phaleria macrocarpa; plant leaf; protein expression; ulcerative colitis; upregulation |
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09753575 |
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