Publikasi Scopus FKUI Tahun 2010 s/d 2020 (data Per 3 Februari 2021)

Pawitan J.A., Bustami A., Damayanti L., Antarianto R.D., Swantari N.M.
6508348067;57192888839;6506640886;57190862806;57192911834;
Effect of adipose tissue processing procedures in culture result: A study preliminary
2011
Medical Journal of Indonesia
20
1
15
59
Department of Histology, Faculty of Medicine, University of Indonesia, Indonesia; Immunology and Endocrinology Integrated Laboratory, Faculty of Medicine, University of Indonesia, Indonesia; Department of Surgery, Faculty of Medicine, University of Indonesia, Indonesia
Pawitan, J.A., Department of Histology, Faculty of Medicine, University of Indonesia, Indonesia; Bustami, A., Immunology and Endocrinology Integrated Laboratory, Faculty of Medicine, University of Indonesia, Indonesia; Damayanti, L., Department of Histology, Faculty of Medicine, University of Indonesia, Indonesia; Antarianto, R.D., Department of Histology, Faculty of Medicine, University of Indonesia, Indonesia; Swantari, N.M., Department of Surgery, Faculty of Medicine, University of Indonesia, Indonesia
Background: There are various methods of processing adipose tissue before culture, depending on the adipose tissue samples. The aim of this study is to compare several modifications of culturing and sub-culturing procedures of adipose tissue to fit the condition in our laboratory. Method: This is a descriptive study that was done in the Immunology and Endocrinology Integrated Laboratory, University of Indonesia, from October 2009 to April 2010. Three adipose tissue processing procedures, various amount of seeding and two subculture methods were compared in term of cell yield and time needed. In the first procedure, collagenase-1 digestion was done in 30minutes, cell seeding were 24,000 and 36,000 per flask; in the second procedure, collagenase-1 digestion was done in 60minutes, cell seeding were 24,000, 48,000, and 72,000 per flask; and in the third procedure, the adipose tissue remnants from the first procedure were again digested for another 45 minutes, cell seeding were 74,000, and 148,000 per flask. Difference in subculture methods were the presence or absence of washing step. Result: Procedure 1 yielded the lowest amount of cell, and after culture, the cells grew very slow, and was contaminated before harvest of primary culture. Procedure-2 and -3 succeeded to yield primary cultures. Some of the cultures were contaminated, so that further subculture was not applicable, and only one tissue processing procedure (procedure 2: 60 minute collagenase-1 digestion, without lysis buffer, cell seeding 48,000 and 72,000) could complete the three subcultures. Though some of the procedures could not be completed, final result could be concluded. Conclusion: In this preliminary study, 60 minute colagenase-1 digestion with intermittent shaking every 5 minutes and cell seeding around 50,000 or more, followed by subculture method without washing step gave the best result. © 2011, Faculty of Medicine, Universitas Indonesia. All rights reserved.
Collagenase-1; Primary culture; Stromal-vascular fraction; Subculture
collagenase 3; adipose tissue; adipose tissue processing; Article; cell growth; cell seeding; cryopreservation; descriptive research; human; human cell; Indonesia; lysis; organ culture; primary culture
Faculty of Medicine, Universitas Indonesia
08531773
Article
Q3

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