Publikasi Scopus 2024 per tanggal 31 Agustus 2024 (707 artikel)

Selisana S.M.G.; Chen X.; Mahfudhoh E.; Bowolaksono A.; Rozaliyani A.; Orihara K.; Kajiwara S.
Selisana, Shiela Marie Gines (57194495441); Chen, Xinyue (57192119643); Mahfudhoh, Eny (59207100400); Bowolaksono, Anom (57205093224); Rozaliyani, Anna (57203065912); Orihara, Kanami (22234653000); Kajiwara, Susumu (55759474200)
57194495441; 57192119643; 59207100400; 57205093224; 57203065912; 22234653000; 55759474200
Alteration of β-glucan in the emerging fungal pathogen Candida auris leads to immune evasion and increased virulence
2024
Medical Microbiology and Immunology
213
1
13
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School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Kanagawa, Yokohama, 226-8501, Japan; Department of Biology, Faculty of Mathematics and Science, University of Indonesia, Depok, 16424, Indonesia; Faculty of Medicine, University of Indonesia, Jakarta, 10430, Indonesia
Selisana S.M.G., School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Kanagawa, Yokohama, 226-8501, Japan; Chen X., School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Kanagawa, Yokohama, 226-8501, Japan; Mahfudhoh E., School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Kanagawa, Yokohama, 226-8501, Japan; Bowolaksono A., Department of Biology, Faculty of Mathematics and Science, University of Indonesia, Depok, 16424, Indonesia; Rozaliyani A., Faculty of Medicine, University of Indonesia, Jakarta, 10430, Indonesia; Orihara K., School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Kanagawa, Yokohama, 226-8501, Japan; Kajiwara S., School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Kanagawa, Yokohama, 226-8501, Japan
Candida auris is an emerging pathogenic yeast that has been categorized as a global public health threat and a critical priority among fungal pathogens. Despite this, the immune response against C. auris infection is still not well understood. Hosts fight Candida infections through the immune system that recognizes pathogen-associated molecular patterns such as β-glucan, mannan, and chitin on the fungal cell wall. In this study, levels of β-glucan and mannan exposures in C. auris grown under different physiologically relevant stimuli were quantified by flow cytometry-based analysis. Lactate, hypoxia, and sublethal concentration of fluconazole trigger a decrease in surface β-glucan while low pH triggers an increase in β-glucan. There is no inverse pattern between exposure levels of β-glucan and mannan in the cell wall architecture among the three clades. To determine the effect of cell wall remodeling on the immune response, a phagocytosis assay was performed, followed by quantification of released cytokines by ELISA. Lactate-induced decrease in β-glucan leads to reduced uptake of C. auris by PMA-differentiated THP-1 and RAW 264.7 macrophages. Furthermore, reduced production of CCL3/MIP-1⍺ but not TNF-⍺ and IL-10 were observed. An in vivo infection analysis using silkworms reveals that a reduction in β-glucan triggers an increase in the virulence of C. auris. This study demonstrates that β-glucan alteration occurs in C. auris and serves as an escape mechanism from immune cells leading to increased virulence. © The Author(s) 2024.
Candida auris; Cell wall remodeling; Phagocytosis; Silkworm; β-glucan
Animals; beta-Glucans; Candida auris; Candidiasis; Cell Wall; Cytokines; Disease Models, Animal; Humans; Immune Evasion; Lactic Acid; Macrophages; Mannans; Mice; Phagocytosis; RAW 264.7 Cells; THP-1 Cells; Virulence; beta glucan; cytokine; fluconazole; interleukin 10; lactic acid; macrophage inflammatory protein 1alpha; mannan; phorbol 12 acetate 13 myristate; tumor necrosis factor; beta glucan; cytokine; mannan; Article; Bombyx mori; Candida auris; controlled study; cytokine release; enzyme linked immunosorbent assay; flow cytometry; fungal cell wall; fungal strain; fungal virulence; fungus growth; hypoxia; immune evasion; immune response; immunocompetent cell; in vivo study; nonhuman; pH; phagocytosis; RAW 264.7 cell line; sublethal concentration; THP-1 cell line; animal; Candida auris;
Tokyo Institute of Technology, TITECH
This work was supported by internal research funds from Tokyo Institute of Technology.
Springer Science and Business Media Deutschland GmbH
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Article
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