Publikasi Scopus FKUI 2021 per tanggal 31 Agustus 2021 (582 artikel)

Publikasi Scopus FKUI 2021 per tanggal 31 Agustus 2021 (582 artikel)

Sunarno, Khariri, Muna F., Sariadji K., Rukminiati Y., Febriyana D., Febrianti T., Saraswati R.D., Susanti I., Puspandari N., Karuniawati A., Malik A., Soebandrio A.
57199647190;57222528104;57218911032;57199654249;57214868942;57222530233;57222528085;57214871905;57222520009;56786591900;54886816200;35079198800;8602893200;
New approach for the identification of potentially toxigenic Corynebacterium sp. using a multiplex PCR assay
2021
Journal of Microbiological Methods
184
106198
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103000221&doi=10.1016%2fj.mimet.2021.106198&partnerID=40&md5=3183ff3090d2d560fdf7771ab93c7a66
Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Division of Microbiology and Biotechnology Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia; Eijkman Institute for Molecular Biology, Jakarta, Indonesia
Sunarno, Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Khariri, Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Muna, F., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Sariadji, K., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Rukminiati, Y., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Febriyana, D., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Febrianti, T., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Saraswati, R.D., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Susanti, I., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Puspandari, N., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Karuniawati, A., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Malik, A., Division of Microbiology and Biotechnology Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia; Soebandrio, A., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Eijkman Institute for Molecular Biology, Jakarta, Indonesia
In diphtheria laboratory examinations, the PCR test can be applied to isolates and clinical specimens. This study aimed to develop a PCR assay to identify the species and toxigenicity of diphtheria-causing bacteria, including the prediction of some NTTB types. Seven reference isolates, four synthetic DNA samples, 36 stored isolates, and 487 clinical samples used for PCR optimization. The PCR results was confirmed by DNA sequence analysis. The results of the PCR examination of the 7 reference isolates and 36 stored isolates were similar to the results obtained using conventional methods as gold standard, both for diphtheria-causing and non-diphtheria-causing bacteria. The validation of the PCR results using DNA sequence analysis showed that there was no mispriming or misamplification. The multiplex PCR assay developed in this study could correctly identify the species and toxigenicity of diphtheria-causing bacteria, including the prediction of some NTTB types not yet covered by established PCR methods. © 2021 Elsevier B.V.
Diphtheria; dtxR gene; PCR; Potentially toxigenic Corynebacterium
Article; bacterial gene; bacterium identification; controlled study; Corynebacterium; diphtheria; DNA sequence; dtxR gene; gold standard; multiplex polymerase chain reaction; nonhuman; priority journal
Elsevier B.V.
1677012
33713727
Article
Q3
629
8028

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