Publikasi Scopus FKUI 2021 per tanggal 31 Agustus 2021 (582 artikel)

182

Authors_(Last_Name)

Sunarno, Khariri, Muna F., Sariadji K., Rukminiati Y., Febriyana D., Febrianti T., Saraswati R.D., Susanti I., Puspandari N., Karuniawati A., Malik A., Soebandrio A.

Author S Id

57199647190;57222528104;57218911032;57199654249;57214868942;57222530233;57222528085;57214871905;57222520009;56786591900;54886816200;35079198800;8602893200;

Title

New approach for the identification of potentially toxigenic Corynebacterium sp. using a multiplex PCR assay

Year

2021

Source Title

Journal of Microbiological Methods

Volume

184

Issue

Art No

106198

Page Start

Page End

Page Count

Cited By

Link

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103000221&doi=10.1016%2fj.mimet.2021.106198&partnerID=40&md5=3183ff3090d2d560fdf7771ab93c7a66

Affiliations

Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Division of Microbiology and Biotechnology Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia; Eijkman Institute for Molecular Biology, Jakarta, Indonesia

Authors With Affiliations

Sunarno, Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Khariri, Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Muna, F., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Sariadji, K., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Rukminiati, Y., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Febriyana, D., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Febrianti, T., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Saraswati, R.D., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Susanti, I., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Puspandari, N., Centre for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia; Karuniawati, A., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Malik, A., Division of Microbiology and Biotechnology Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia; Soebandrio, A., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Eijkman Institute for Molecular Biology, Jakarta, Indonesia

Abstract

In diphtheria laboratory examinations, the PCR test can be applied to isolates and clinical specimens. This study aimed to develop a PCR assay to identify the species and toxigenicity of diphtheria-causing bacteria, including the prediction of some NTTB types. Seven reference isolates, four synthetic DNA samples, 36 stored isolates, and 487 clinical samples used for PCR optimization. The PCR results was confirmed by DNA sequence analysis. The results of the PCR examination of the 7 reference isolates and 36 stored isolates were similar to the results obtained using conventional methods as gold standard, both for diphtheria-causing and non-diphtheria-causing bacteria. The validation of the PCR results using DNA sequence analysis showed that there was no mispriming or misamplification. The multiplex PCR assay developed in this study could correctly identify the species and toxigenicity of diphtheria-causing bacteria, including the prediction of some NTTB types not yet covered by established PCR methods. © 2021 Elsevier B.V.

Author Keywords

Diphtheria; dtxR gene; PCR; Potentially toxigenic Corynebacterium

Index Keywords

Article; bacterial gene; bacterium identification; controlled study; Corynebacterium; diphtheria; DNA sequence; dtxR gene; gold standard; multiplex polymerase chain reaction; nonhuman; priority journal

Publisher

Elsevier B.V.

Issn

1677012

Isbn

Pubmed Id

33713727

Document Type

Article

Sjr_Best_Quartile

Sjr

629

Sjr Rank

8028