Publikasi Scopus FKUI 2021 per tanggal 31 Agustus 2021 (582 artikel)

Soeroso N.N., Ananda F.R., Pradana A., Tarigan S.P., Syahruddin E., Noor D.R.
57201670271;57201666959;57202578193;6602815057;6507688750;57214096801;
The absence of mutations in the exon 2 kras gene in several ethnic groups in north sumatra may not the main factor for lung cancer
2021
Acta Informatica Medica
29
2
108
112
Thoracic Oncology Division, Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara, Indonesia; Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara, Indonesia; Thoracic Oncology Division, Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Indonesia; Human Cancer Research Center, Indonesian Medical Education and Research Institute, Universitas Indonesia, Indonesia
Soeroso, N.N., Thoracic Oncology Division, Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara, Indonesia; Ananda, F.R., Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara, Indonesia; Pradana, A., Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara, Indonesia; Tarigan, S.P., Thoracic Oncology Division, Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara, Indonesia; Syahruddin, E., Thoracic Oncology Division, Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Indonesia, Human Cancer Research Center, Indonesian Medical Education and Research Institute, Universitas Indonesia, Indonesia; Noor, D.R., Human Cancer Research Center, Indonesian Medical Education and Research Institute, Universitas Indonesia, Indonesia
Background: Rat Sarcoma (RAS) protein encoded Guanosine Triphosphate (GTP-ase) activity, known as a switch of cell proliferation. The mutation of this protein alters the early stage of carcinogenesis and along with the interaction with other oncogene drivers and environmental factors affect the clinical characteristics and prognosis in cancer patients, particularly lung cancer. Objective: This study aims to determine the Kristen Rat Sarcoma (KRAS) mutation in lung cancer patients in North Sumatera and evaluate factors that might contribute in the development of lung cancer in the absence of KRAS mutation. Methods: This was a retrospective cohort study enrolled 44 subjects age > 18 year with the diagnosis of lung cancer. Histopathology preparation was obtained from surgery, bronchoscopy, and percutaneus needle biopsy then formed as paraffin-block. KRAS mutation was analyzed using Polymerase Chain Reaction (PCR) method with specific primer of exon 2 for evaluating the expression of RAS protein then continued with Sanger Sequencing Method at 12th and 13th codon. Results: The majority of subjects were male, age > 40 years old, bataknese, heavy smoker, with Adenocarcinoma. Almost all the subjects showed the expression of exon 2 of RAS protein in PCR examinations. However, Sequencing analysis using Bioedit Software, BLASTs and Finch T showed GGT GGC as protein base 219-224 which represented 12th and 13th Codon 12 and 13. The results interpreted there was no mutations of exon 2 of KRAS in North Sumatera Population. Conclusion: The absence of KRAS mutation in exon 2 in several ethnics in North Sumatera populations was not the main factors of lung cancer. © 2021 Noni Novisari Soeroso, Fannie Rizki Ananda, Andika Pradana, Setia Putra Tarigan, Elisna Syahruddin, Dimas Ramadhian Noor
Ethnic variation; Exon 2; Indonesian; KRAS mutation; Lung cancer
Avicena Publishing
3538109
Article
Q3
267
15980