Publikasi Scopus FKUI 2021 per tanggal 31 Agustus 2021 (582 artikel)

397

Authors_(Last_Name)

Sundari A.M., Iffanolida P.A., Mutia K., Muna N., Mansyur E., Hestiantoro A., Wiweko B., Fauzi M., Bowolaksono A.

Author S Id

57222070793;57195937128;57195936432;57195936218;57190493343;8743255100;43061741400;57211523956;57205093224;

Title

Carrier RNA (cRNA) Enhances dsDNA Recovery Extracted from Small Volume Spent Embryo Culture Medium

Year

2021

Source Title

International Journal on Advanced Science, Engineering and Information Technology

Volume

Issue

Art No

Page Start

1203

Page End

1208

Page Count

Cited By

Link

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85110438610&doi=10.18517%2fijaseit.11.3.13591&partnerID=40&md5=970d0465dee0414f7c917de0738ee65b

Affiliations

Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences of Universitas Indonesia, West Java, Depok, 16424, Indonesia; Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine Universitas Indonesia, Jakarta, 10430, Indonesia; Yasmin In Vitro Fertilization Clinic, Cipto Mangunkusumo General Hospital, Jakarta, 10430, Indonesia; Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, 10430, Indonesia; Department of Diabetes Endocrinology and Nutrition, Graduates School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan

Authors With Affiliations

Sundari, A.M., Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences of Universitas Indonesia, West Java, Depok, 16424, Indonesia; Iffanolida, P.A., Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine Universitas Indonesia, Jakarta, 10430, Indonesia; Mutia, K., Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences of Universitas Indonesia, West Java, Depok, 16424, Indonesia, Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine Universitas Indonesia, Jakarta, 10430, Indonesia; Muna, N., Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine Universitas Indonesia, Jakarta, 10430, Indonesia; Mansyur, E., Yasmin In Vitro Fertilization Clinic, Cipto Mangunkusumo General Hospital, Jakarta, 10430, Indonesia; Hestiantoro, A., Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, 10430, Indonesia; Wiweko, B., Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, 10430, Indonesia; Fauzi, M., Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences of Universitas Indonesia, West Java, Depok, 16424, Indonesia, Department of Diabetes Endocrinology and Nutrition, Graduates School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan; Bowolaksono, A., Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences of Universitas Indonesia, West Java, Depok, 16424, Indonesia

Abstract

Embryo spent culture medium has been intensively investigated, considering its promising feature for non-invasive bioanalytical techniques in in-vitro fertilization (IVF). Despite, isolating DNA from such samples is quite challenging due to its small volume. Carrier RNA is reported to exhibit DNA retrieval effects and commonly employed in various limited biological samples, but there are no reports regarding its benefit on embryo media. Therefore, we aim to evaluate the competence of cRNA on isolated DNA from embryo medium and analyzed its optimal volume as there are also no records respecting its ideal volume to obtain decent outcomes. Results showed that cRNA significantly increases DNA amounts in the cRNA treated group (p<0.001), but the D-4/D-5 medium yielded similar (p=0.684). Pearson test demonstrated no correlation between cRNA volume vs. total retrieved DNA (r=0.760, p=0.80), and Whole Genome Amplification (WGA) was shown to increase DNA in the treated group (p=0.022), but not in the untreated group (p=0.128). Additionally, electrophoresis successfully resulting in a thick and thin band of TH01 locus signifies the cRNA competence. In conclusion, our study suggests that cRNA addition is essential in embryo medium extraction as it increases initial DNA that crucial for downstream application. However, the optimal volume could not be determined in the current study since the initial amount of DNA in the medium is unknown. Obtained findings are expected to be a new input for subsequent research on DNA extraction. © 2021. All Rights Reserved.

Author Keywords

Assisted reproductive technology; carrier RNA; in-vitro fertilization; spent embryo medium

Index Keywords

Publisher

Insight Society

Issn

20885334

Isbn

Pubmed Id

Document Type

Article

Sjr_Best_Quartile

Sjr

216

Sjr Rank

18393