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Publikasi Scopus 2024 per tanggal 30 April 2024 (334 artikel)
Publikasi Scopus 2024 per tanggal 30 April 2024 (334 artikel)
Desire, Itfetania Aemilly (58934496000); Luqman, Muhammad (58934570000); Puspitasari, Yulianna (57208229012); Tyasningsih, Wiwiek (57204721266); Wardhana, Dhandy Koesoemo (57207833139); Meles, Dewa Ketut (57204721968); Dhamayanti, Yeni (57204787302); Permatasari, Dian Ayu (57217108986); Witaningrum, Adiana Mutamsari (56151088600); Perwitasari, Agnes Dwi Sis (58934496100); Raharjo, Hartanto Mulyo (57204733784); Ayuti, Siti Rani (57215069310); Kurniawan, Shendy Canadya (57863649900); Kamaruzaman,
First detection of bovine tuberculosis by Ziehl–Neelsen staining and polymerase chain reaction at dairy farms in the Lekok Sub-District, Pasuruan Regency, and Surabaya region, Indonesia
2024
Veterinary World
17
3
577
584
7
0
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85187576911&doi=10.14202%2fvetworld.2024.577-584&partnerID=40&md5=b14930e8ae596f073e845a7f7cad1312
Bachelor Program of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, East Java, Surabaya, Indonesia; Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, East Java, Surabaya, Indonesia; Division of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, East Java, Surabaya, Indonesia; Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, East Java, Surabaya, Indonesia; Division of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, East Java, Surabaya, Indonesia; Department of Tuberculosis, Institute Tropical Disease, Universitas Airlangga, East Java, Surabaya, Indonesia; Biochemistry Laboratory, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, Indonesia; Master Program of Animal Sciences, Department of Animal Sciences, Specialisation in Molecule, Cell and Organ Functioning, Wageningen University and Research, Wageningen, Netherlands; Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kelantan, Malaysia; Doctoral Program of Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Background and Aim: Bovine tuberculosis (TB) is a zoonotic disease of great public health importance, particularly in Indonesia, where control measures are limited or are not implemented. This study aimed to detect the presence of Mycobacterium pathogens in milk samples from dairy cattle in Pasuruan regency and Surabaya City, East Java, using Ziehl–Neelsen acid-fast staining and polymerase chain reaction (PCR). Materials and Methods: Milk samples were aseptically collected from 50 cattle in the Lekok Subdistrict, Pasuruan Regency, and 44 from dairy farms in the Lakarsantri Subdistrict, Wonocolo Subdistrict, Mulyorejo Subdistrict, and Kenjeran Subdistrict, Surabaya, East Java. To detect Mycobacteria at the species level, each sample was assessed by Ziehl–Neelsen staining and PCR using the RD1 and RD4 genes. Results: The results of PCR assay from 50 samples in Lekok Subdistrict, Pasuruan Regency showed that 30 samples (60%) were positive for Mycobacterium tuberculosis and two samples (4%) were positive for Mycobacterium bovis, although Ziehl–Neelsen staining did not show the presence of Mycobacterium spp. In the Surabaya region, 31 samples (70.45%) were positive for M. tuberculosis and three samples (6.8%) were positive for M. bovis. Six samples (13.63%) from all PCR-positive samples could be detected microscopically with Ziehl–Neelsen. Conclusion: The presence of bovine TB in this study supports the importance of using a molecular tool alongside routine surveillance for a better understanding of the epidemiology of bovine TB in East Java. © 2024 Veterinary World. All rights reserved.
agar gel electrophoresis; animal experiment; Article; bacterial gene; bovine tuberculosis; controlled study; dairy cattle; dairying; DNA extraction; gene amplification; Indonesia; milk; molecular diagnosis; multiplex polymerase chain reaction; Mycobacterium bovis; Mycobacterium tuberculosis; nonhuman; RD1 gene; RD4 gene; staining; Ziehl-Neelsen staining
Universitas Airlangga, UNAIR; Penelitian Dosen Pemula Universitas Airlangga Tahun 2022, (251/UN3/2022)
This study was supported by the Rector of Universitas Airlangga, Surabaya, Indonesia, who provided funding for this study by Penelitian Dosen Pemula Universitas Airlangga Tahun 2022 (Grant number: 251/UN3/2022). The authors would also like to thank the Food Security and Agriculture Departments of Surabaya and Pasuruan for their support and cooperation.
Veterinary World
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