Riset dan Pengabdian Masyarakat
Do Research Sosial Service and Innovation
Publikasi Scopus 2024 per tanggal 30 April 2024 (334 artikel)
Publikasi Scopus 2024 per tanggal 30 April 2024 (334 artikel)
Kusmiati, Kusmiati (58639246100); Fanani, Asrul (57950675000); Nurkanto, Arif (36025675800); Purnaningsih, Ismu (57214117743); Mamangkey, Jendri (57210287811); Ramadhani, Indriati (57195557566); Nurcahyanto, Dian Alfian (54890727300); Simanjuntak, Partomuan (16158253100); Afiati, Fifi (57201406105); Irawan, Herman (58650668500); Puteri, Ade Lia (58945342700); Ewaldo, Muhammad Farrel (58945342800); Juanssilfero, Ario Betha (57189369470)
Profile and in silico analysis of metabolite compounds of the endophytic fungus Alternaria alternata K-10 from Drymoglossum piloselloides as antioxidants and antibacterials
2024
Heliyon
10
6
e27978
0
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85188135761&doi=10.1016%2fj.heliyon.2024.e27978&partnerID=40&md5=4b68e80e8824e8af0682e1393dac3120
Research Center for Biosystematics and Evolution- Research Organization for Life Sciences and Environment, The National Research and Innovation Agency (BRIN), Indonesia; Research and Education Center for Bioinformatics, Indonesia Institute of Bioinformatics, Malang, 65162, Indonesia; Directorate of Scientific Collection Management, The National Research and Innovation Agency (BRIN)- KST Soekarno, Jl Raya Bogor Km 46, Cibinong Bogor, 16911, Indonesia; Department of Biology Education, Faculty of Education and Teacher Training, Universitas Kristen Indonesia, Jakarta, Indonesia; Research Center for Pharmaceutical Ingredient and Traditional Medicine, National Research and Innovation Agency (BRIN), Indonesia; Research Center for Applied Microbiology-Research Organization for Life Sciences and Environment, The National Research and Innovation Agency (BRIN), Indonesia; Research Center for Genetic Engineering, Research Organization for Life Sciences and Environment, National Research and Innovation Agency (BRIN), KST Soekarno, Cibinong, Bogor, Indonesia; Master's Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia Jl. Salemba Raya – Jakarta Pusat, Indonesia
Endophytic fungi are known for producing secondary metabolites with valuable biological activities, including antiviral, anticancer, antibacterial, and antioxidant properties. This study aims to evaluate an endophytic fungus from Dragon Scales leaves (Drymoglossum piloselloides) and analyze its metabolites as antioxidants and antibacterials. In this study, an endophytic fungus was isolated from the leaves of Dragon Scales (D. piloselloides) and identified using molecular analysis of the Internal Transcribed Spacer (ITS) ribosomal RNA locus. The fungus was authenticated as Alternaria alternata strain K-10. Crude extracts were obtained using n-hexane and ethyl acetate and analyzed via GC-MS Shimadzu-QP 2010 Ultra with NIST spectral library. Antibacterial activity was observed against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using the paper disc method, showing inhibition zones of 8.7–9.3 mm and 8.8–9.4 mm for ethyl acetate and n-hexane extracts, respectively. Ethyl acetate and n-hexane extracts exhibited strong antioxidant potential against 2,2-diphenyl-1-picrylhydrazil (DPPH) radical (IC50 values of 50.99 μg mL−1 and 74.44 μg mL−1, respectively). GC–MS analysis revealed 40 compounds in both extracts, some of which, including 2-ethylhexyl ester benzoic acid, benzo-b-dihydropyran-6-hydroxy-4-4-5-7-8-pentamethyl, diethyl phthalate, and octadecanoic acid, were identified through in silico analysis and found to possess antioxidant properties. These findings hold implications for potential applications of the plant and its biological constituent to be developed as lead compounds in the medical sector. © 2024 The Authors
RIIM; Lembaga Pengelola Dana Pendidikan, LPDP; Rispro LPDP; Priority grant of Drug and Vaccine by Research Organization for Health BRIN; Badan Riset dan Inovasi Nasional, BRIN; Riset Inovasi Untuk Indonesia Maju, Rispro LPDP Indonesia, (RIIM-B-803/II.7.5/FR/6/2022, B-1373/ III.5/PR.03)
Funding text 1: This work was partially supported by a grant from RIIM (Riset Inovasi u ntuk Indonesia Maju), Rispro LPDP (Lembaga Pengelola Dana Pendidikan)Indonesia (RIIM-B-803/II.7.5/FR/6/2022 and B-1373/ III.5/PR.03. June 08, 2022) and Priority grant of Drug and Vaccine by Research Organization for Health BRIN (DIPA). The authors would like to thank Deputy for Research and Innovation Infrastru
Elsevier Ltd
24058440
Article
Q1
609
8422