Publikasi Scopus FKUI 2021 per tanggal 30 April 2021 (299 artikel)

Rizal, Syaidah R., Aqsha Z.M., Josephin A., Pakpahan V.M.
57222623832;55418885900;57222626455;57222619624;57222621602;
Characterization, differentiation, and population doubling time of Wharton's jelly mesenchymal stem cells (WJ-MSCs) in passage 5 and 8
2021
AIP Conference Proceedings
2344
040002
Biomedical Engineering Program, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, West Java, 16424, Indonesia; Department of Histology, Faculty of Medicine, Universitas Indonesia, Kampus UI Depok, West Java, 16424, Indonesia; Undergraduate Program in Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, West Java, 16424, Indonesia
Rizal, Biomedical Engineering Program, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, West Java, 16424, Indonesia; Syaidah, R., Department of Histology, Faculty of Medicine, Universitas Indonesia, Kampus UI Depok, West Java, 16424, Indonesia; Aqsha, Z.M., Undergraduate Program in Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, West Java, 16424, Indonesia; Josephin, A., Undergraduate Program in Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, West Java, 16424, Indonesia; Pakpahan, V.M., Undergraduate Program in Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, West Java, 16424, Indonesia
Wharton's jelly mesenchymal stem cells (WJ-MSCs) is proposed to be the best source of mesenchymal stem cells (MSCs). It has better self-renewal and differentiation capacity compare to adult stem cells such as bone marrow- derived stem cells (BM-MSCs). However, their characteristics in different passages do not yet well described. This research aims to isolate and characterize WJ-MSCs with regard to their proliferation capacity, immunophenotypes, and differentiation capacity. The WJ-MSCs were isolated by the explant method. The cultured WJ-MSCs at P5 and P8 were then characterized using flow cytometric analysis with regard to its surface marker, which are CD44, CD90, CD105, CD73, and negative lineages. The proliferation capacity of WJ-MSCs was analyzed with the population doubling time method and the characterized cells were differentiated into three different lineages. The result revealed that the isolated cells highly express MSCs surface marker with >98% for all surface markers and 0.00% for negative lineages. Isolated WJ-MSCs also exhibited a high proliferation capacity with a range of 1.06 to 1.59 days. Both passages revealed excellent differentiation capacity into chondrocytes, adipocytes, and osteocytes. © 2021 Author(s).
characterization; differentiation; Mesenchymal stem cells; proliferation; Wharton's jelly
American Institute of Physics Inc.
0094243X
9.78074E+12
Conference Paper
-
190
19092