Publikasi Scopus 926 artikel (Per 14 Maret 2022)

Hamid A.R.A.H., Luna-Velez M.V., Dudek A.M., Jansen C.F.J., Smit F., Aalders T.W., Verhaegh G.W., Schaafsma E., Sedelaar J.P.M., Schalken J.A.
57202054669;56667692600;48761226600;8515960200;7005490663;6603571474;6603760728;6603552898;6602103585;7101847178;
Molecular Phenotyping of AR Signaling for Predicting Targeted Therapy in Castration Resistant Prostate Cancer
2021
Frontiers in Oncology
11
721659
Department of Urology, Radboud University Medical Center, Nijmegen, Netherlands; Department of Urology, Ciptomangunkusumo Hospital, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; NovioGendix, Nijmegen, Netherlands; Department of Pathology, Radboud University Medical Center, Nijmegen, Netherlands
Hamid, A.R.A.H., Department of Urology, Radboud University Medical Center, Nijmegen, Netherlands, Department of Urology, Ciptomangunkusumo Hospital, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Luna-Velez, M.V., Department of Urology, Radboud University Medical Center, Nijmegen, Netherlands; Dudek, A.M., Department of Urology, Radboud University Medical Center, Nijmegen, Netherlands; Jansen, C.F.J., Department of Urology, Radboud University Medical Center, Nijmegen, Netherlands; Smit, F., NovioGendix, Nijmegen, Netherlands; Aalders, T.W., Department of Urology, Radboud University Medical Center, Nijmegen, Netherlands; Verhaegh, G.W., Department of Urology, Radboud University Medical Center, Nijmegen, Netherlands; Schaafsma, E., Department of Pathology, Radboud University Medical Center, Nijmegen, Netherlands; Sedelaar, J.P.M., Department of Urology, Radboud University Medical Center, Nijmegen, Netherlands; Schalken, J.A., Department of Urology, Radboud University Medical Center, Nijmegen, Netherlands
Castration-resistant prostate cancer (CRPC) is defined by resistance of the tumor to androgen deprivation therapy (ADT). Several molecular changes, particularly in the AR signaling cascade, have been described that may explain ADT resistance. The variety of changes may also explain why the response to novel therapies varies between patients. Testing the specific molecular changes may be a major step towards personalized treatment of CRPC patients. The aim of our study was to evaluate the molecular changes in the AR signaling cascade in CRPC patients. We have developed and validated several methods which are easy to use, and require little tissue material, for exploring AR signaling pathway changes simultaneously. We found that the AR signaling pathway is still active in the majority of our CRPC patients, due to molecular changes in AR signaling components. There was heterogeneity in the molecular changes observed, but we could classify the patients into 4 major subgroups which are: AR mutation, AR amplification, active intratumoral steroidogenesis, and combination of AR amplification and active intratumoral steroidogenesis. We suggest characterizing the AR signaling pathway in CRPC patients before beginning any new treatment, and a recent fresh tissue sample from the prostate or a metastatic site should be obtained for the purpose of this characterization. © Copyright © 2021 Hamid, Luna-Velez, Dudek, Jansen, Smit, Aalders, Verhaegh, Schaafsma, Sedelaar and Schalken.
androgen receptor; castration-resistant prostate cancer; gene amplification; gene mutation; splice variant; steroidogenic enzymes
aldo keto reductase family 1 member C3; androgen receptor; antiandrogen; estrogen; genomic DNA; gonadorelin agonist; Article; castration resistant prostate cancer; cell culture; controlled study; gene amplification; gene expression; gene mutation; human; human tissue; immunohistochemistry; male; molecularly targeted therapy; mRNA expression level; orchiectomy; phenotype; prostate hypertrophy; real time polymerase chain reaction; RNA extraction; signal transduction
Frontiers Media S.A.
2234943X
Article
Q1
1834
1574