Publikasi Scopus 926 artikel (Per 14 Maret 2022)

Kusmardi K., Situmorang N.Y., Zuraidah E., Estuningtyas A., Tedjo A.
56966625300;57357530500;57357911500;55650360200;57189320451;
The effect of mahkota dewa (Phaleria macrocarpa) leaf extract on the Mucin 1 expression in mice colonic epithelial cells induced by dextran sodium sulfate (DSS)
2021
Pharmacognosy Journal
13
6
1509
1515
Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Drug Development Research Center, Indonesia Medical Education and Resesarch Institute (IMERI), Universitas Indonesia, Jakarta, Indonesia; Human Cancer Research Center, IMERI, Universitas Indonesia, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Kusmardi, K., Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Drug Development Research Center, Indonesia Medical Education and Resesarch Institute (IMERI), Universitas Indonesia, Jakarta, Indonesia, Human Cancer Research Center, IMERI, Universitas Indonesia, Jakarta, Indonesia; Situmorang, N.Y., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Zuraidah, E., Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Estuningtyas, A., Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Tedjo, A., Drug Development Research Center, Indonesia Medical Education and Resesarch Institute (IMERI), Universitas Indonesia, Jakarta, Indonesia, Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Background: Inflammatory bowel disease is a chronic inflammation caused by the malignant inflammation response and if not treated, could lead to colorectal cancer. One of the researched treatment is mahkota dewa (Phaleria macrocarpa) leaf extract that has flavonoid compound known to reduce inflammation. This study was aimed to prove that mahkota dewa leaf extract could reduce inflammation of mice colon induced with dextran sodium sulfate (DSS) and observe MUC1 expression from colon epithelial crypt of Lieberkuhn. Methods: This was a laboratory experiment using biological material (paraffin block) taken from 28 mice and divided into 5 groups: normal, aspirin, low and high dose mahkota dewa, and negative control. They were processed into immunohistochemistry and stained microscopic slides. Afterwards, they were observed with 400x magnification and 5 field-of-view of mice colon crypt of lieberkuhn. Then MUC1 expression was counted using ImageJ to obtain mean immunohistochemistry score and analyzed with SPSS. Results: There were significant reduction of MUC1 expressions from normal, aspirin, and high dose mahkota dewa groups compared to the negative control group. The result shown MUC1 expression from high dose mahkota dewa (M=149.90,SD=3.81) and aspirin (M=158.92,SD=5.28) were closer to normal group (M=148.02,SD=5.28). There were no significant results between negative (M=175.39,SD=14.30) and low dose mahkota dewa group (M=149.90,SD=5.02). Conclusion: There was a reduction of MUC1 expression in DSS-induced mice colonic epithelial cells for high dose mahkota dewa group. This shown that high dosage mahkota dewa leaf extract could reduce inflammation like aspirin. © 2021 Phcogj.Com.
Anti-inflammatory agent; Colon epithelial cell; Inflammatory bowel disease; Mahkota dewa (Phaleria macrocarpa); MUC 1 expression
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Article
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