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Publikasi Scopus 926 artikel (Per 14 Maret 2022)
Publikasi Scopus 926 artikel (Per 14 Maret 2022)
Effect of Cell Culture Medium on the Proliferation and Stemness of CD24-/CD44+ Human Breast Cancer Stem Cells
2021
Indonesian Biomedical Journal
13
4
355
363
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123550895&doi=10.18585%2fINABJ.V13I4.1674&partnerID=40&md5=f0c363bff3a069d364bc71970a109469
Master’s Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia; Molecular Biology and Proteomics Core Facilities, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya, Kenari, Jakarta, 10430, Indonesia
BACKGROUND: Cancer stem cells (CSCs) is defined as tumor initiating cells within tumor that maintain stemness properties and tumorigenicity. Extracellular pH of CSCs in in vitro condition is important for supporting cell proliferation which may also regulate the expression of stemness markers such as OCT4. This work aimed to examine the effect of cell culture media on the proliferation and stemness of human breast cancer stem cells (BCSCs). RESULTS: Our results showed a higher viable cell number and proliferation of BCSCs in DMEM/F-12 HEPES (-) compared to HEPES (+) medium until 4 day incubation. OCT4 mRNA and protein level, as well as MFU of BCSCs were significantly higher in HEPES (-) compared to HEPES (+) medium on day 2. CONCLUSION: DMEM/F-12 medium without HEPES facilitates CD24-/CD44+ BCSCs to have higher proliferation and stemness on day 2 incubation compared to those with HEPES. © 2021. All Rights Reserved.
Prodia Education and Research Institute
20853297
Article
Q4
160
22228