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Publikasi Scopus 926 artikel (Per 14 Maret 2022)
Publikasi Scopus 926 artikel (Per 14 Maret 2022)
Analysis of dna methylation level and mrna expression of transient receptor ankyrin member 1 (Trpa1) in endometriosis-associated pain
2021
Asia-Pacific Journal of Molecular Biology and Biotechnology
29
3
1
10
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85110656378&partnerID=40&md5=011cfc4031284fa4520681b99cc9b930
Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Human Reproductive, Infertility, and Family Planning Cluster, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Endometriosis Center, Fatmawati Hospital, Jakarta, Indonesia; Neurology Department, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Undergraduate Program in Biological Sciences, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Jakarta, Indonesia; Medical Biology Department, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Midwifery, Politeknik Kesehatan Palembang, Ministry of Health, Palembang, Indonesia
Transient Receptor Ankyrin Member 1 (TRPA1) is an ion channel family protein that regulates pain sensation through sensory neurons' activity. This study's purpose to analyzes the DNA methylation and mRNA expression level of the TRPA1 gene in endometriosis and its correlation with pain level. Twenty samples of peritoneal endometriosis and endometrial samples were obtained from women with endometriosis, which was subsequently compared to 20 endometrial samples of women without endometriosis. The DNA methylation level of TRPA1 was analyzed using Methylation-specific PCR (MS-PCR) and ImageJ software, while the mRNA expression of TRPA1 was analyzed using qRT-PCR. Furthermore, the pain level was measured using the numeric rating scale (NRS) by interviewing all the women. This study showed that there was a significant difference in the mRNA expression of TRPA1 in peritoneal endometriosis. The TRPA1 was unmethylated in both peritoneal and endometrial samples in endometriosis. However, DNA Methylation level of TRPA1 in peritoneal and endometrial of endometriosis compared to normal endometrial were no significant difference. Additionally, there was no correlation between DNA methylation level and mRNA expression level of TRPA1 in all samples, along with the endometriosis-associated pain. © 2021, University of Malaya. All rights reserved.
University of Malaya
01287451
Article
Q4
137
24544