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417 |
Yunir E., Soewondo P., Soelistijo S.A., Rudijanto A. |
36520254800;23475336100;57211324846;23499422500; |
Knowledge and behavior changes in clinician after training of partnership for Diabetes Control in Indonesia |
2021 |
Diabetes and Metabolic Syndrome: Clinical Research and Reviews |
15 |
3 |
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719 |
724 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103636919&doi=10.1016%2fj.dsx.2021.03.012&partnerID=40&md5=69390ed0f1d97f773993433ca4e7d1d0 |
Division of Endocrinology and Metabolism, Department of Internal Medicine Dr. Cipto Mangunkusumo National Referral Hospital, Faculty of Medicine Universitas Indonesia, Jl. Diponegoro No.71, Central Jakarta, 10430, Indonesia; Surabaya Diabetes and Nutrition Centre, Department of Internal Medicine, Universitas Airlangga, Jl. Mayjen Prof. Dr. Moestopo No.47, Surabaya, 60132, Indonesia; Division of Endocrinology and Metabolism, Department of Internal Medicine, Universitas Brawijaya, Jl. Veteran Malang, Malang, 65145, Indonesia |
Yunir, E., Division of Endocrinology and Metabolism, Department of Internal Medicine Dr. Cipto Mangunkusumo National Referral Hospital, Faculty of Medicine Universitas Indonesia, Jl. Diponegoro No.71, Central Jakarta, 10430, Indonesia; Soewondo, P., Division of Endocrinology and Metabolism, Department of Internal Medicine Dr. Cipto Mangunkusumo National Referral Hospital, Faculty of Medicine Universitas Indonesia, Jl. Diponegoro No.71, Central Jakarta, 10430, Indonesia; Soelistijo, S.A., Surabaya Diabetes and Nutrition Centre, Department of Internal Medicine, Universitas Airlangga, Jl. Mayjen Prof. Dr. Moestopo No.47, Surabaya, 60132, Indonesia; Rudijanto, A., Division of Endocrinology and Metabolism, Department of Internal Medicine, Universitas Brawijaya, Jl. Veteran Malang, Malang, 65145, Indonesia |
Background and aims: One of the main determinants of successful diabetes management is the quality of healthcare provider including general practitioner and internist which can be increased through medical training. This study aimed to describe the changes of clinician's knowledge and behavior of comprehensive diabetes management training program around Indonesia. Method: We conducted a three-day training program for general practitioners and internists for 3.5 years, 2013 to 2016. All clinicians invited as voluntary participant to send their patient data from medical record. Each participant was expected to submit a minimum of 25 type 2 diabetes (T2DM) set patient data before and 6 months after training program to analyze the impact of program in physician knowledge and behavior related to diabetes management. Result: 120 of 489 voluntary participants submitted completed baseline data with 4676 patient data. Meanwhile, only 32 participants that submitted completed data of 6 months before after training with 886 patient data. Most of parameters were improve before and after program. The greatest and lowest improvement were on A1c measurement (21%) and smoking assessment (2%). Conclusion: Intensive seminar and training was not enough to empower diabetes management. This research might push the creation of clinical practice program that were tailored to each care facilities and integrated within routine care aimed at continual improvement of its healthcare worker. © 2021 |
Behavior; Diabetes; Knowledge; Training |
fibric acid derivative; hemoglobin A1c; antidiabetic agent; biological marker; glycosylated hemoglobin; hemoglobin A1c protein, human; adult; aged; Article; behavior; behavior change; controlled study; diabetes control; diabetes mellitus; diabetic complication; female; general practitioner; health personnel attitude; human; Indonesia; internist; major clinical study; male; medical education; middle aged; non insulin dependent diabetes mellitus; patient coding; priority journal; retrospective study; smoking; young adult; attitude to health; blood; clinical competence; education; follow up; glucose blood level; health care personnel; internal medicine; medical education; non insulin dependent diabetes mellitus; procedures; professional practice; prognosis; psychology; Adult; Aged; Biomarkers |
Elsevier Ltd |
18714021 |
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33813247 |
Article |
Q2 |
684 |
7304 |
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422 |
Putra M.D., Rahyussalim A.J., Jusman S.W.A., Iswanti F.C., Sadikin M. |
57221617403;55212166100;36518792100;57192920851;36981411100; |
Phagocytosis and the antigen-processing abilities of macrophages derived from monocytes in spinal tuberculosis patients |
2021 |
Journal of Clinical Tuberculosis and Other Mycobacterial Diseases |
23 |
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100215 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099630069&doi=10.1016%2fj.jctube.2021.100215&partnerID=40&md5=2548fbe746aaf9e6e815fb1375351254 |
Master's Program in Biomedical Science, Master's Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia10430, Indonesia; Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta15419, Indonesia; Department of Orthopedics and Traumatology, Faculty of Medicine, Universitas Indonesia-Ciptomangunkusumo Hospital10430, Indonesia; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia10430, Indonesia; Center of Hypoxia and Oxidative Stress Studies, Indonesia |
Putra, M.D., Master's Program in Biomedical Science, Master's Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia10430, Indonesia, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta15419, Indonesia; Rahyussalim, A.J., Department of Orthopedics and Traumatology, Faculty of Medicine, Universitas Indonesia-Ciptomangunkusumo Hospital10430, Indonesia; Jusman, S.W.A., Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia10430, Indonesia, Center of Hypoxia and Oxidative Stress Studies, Indonesia; Iswanti, F.C., Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia10430, Indonesia, Center of Hypoxia and Oxidative Stress Studies, Indonesia; Sadikin, M., Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia10430, Indonesia, Center of Hypoxia and Oxidative Stress Studies, Indonesia |
This study examined the hypothesis that there is an impairment of macrophageal function in spinal TB. We examined macrophageal functions in spinal TB patients. Monocytes were isolated from peripheral blood mononuclear cells (PBMCs) of five spinal TB patients and five healthy persons as control. The isolated monocytes were cultured with stimulation of macrophage colony-stimulating factor (M-CSF) for seven days for maturation. The phagocytic ability of the macrophages derived from monocytes was measured. Also, nitric oxide (NO), myeloperoxidase (MPO), beta-glucuronide, and acid phosphatase activity was investigated. We found that the monocytes collected from patient PBMCs were significantly fewer than those of the control group (2992.103 vs. 6474.103 (cells/mL)). There were also fewer macrophages that had adhered to sheep red blood cells (SRBC) (598.103 vs. 264.103 (cells/mL)). However, NO production (2346 vs. 325.17 (µmol/gram of protein)), and the MPO (570.7 vs. 17.4 (unit/mg), beta-glucuronide (0.149 vs. 0.123 (μmol/hour/100 mg of protein)), and acid phosphatase activities (1776.9 vs. 287.9 (μmol/hour/100 mg of protein)) of the macrophages in the spinal TB group were markedly higher than in the healthy group. Despite the low adhesion to foreign bodies, the intracellular processing of TB macrophages, including oxidative activity and lysosome function, was significantly high. These results suggested the impairment of macrophageal function in spinal TB. Possibly, there is a dominance of innate non-specific immunity in spinal TB infection. © 2021 The Author(s) |
Acid phosphatase; Beta-glucuronidase; Macrophage; Myeloperoxidase; Nitric oxide; Spinal tuberculosis |
acid phosphatase; beta glucuronidase; colony stimulating factor 1; glucuronide; myeloperoxidase; nitric oxide; adult; antigen presentation; Article; cell adhesion; cell maturation; clinical article; controlled study; enzyme activity; female; foreign body; human; human cell; lysosome; macrophage; macrophage culture; macrophage function; male; monocyte; oxidation; phagocytosis; priority journal; sheep erythrocyte; tuberculous spondylitis |
Elsevier Ltd |
24055794 |
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Article |
Q3 |
585 |
8643 |
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423 |
Sitohang I.B.S., Rahmayunita G., Hosfiar V.A., Ninditya S., Augustin M. |
56734569200;57192909174;57219421454;57216741327;57219417020; |
Effectiveness of water as the neutralising agent for glycolic acid peels in skin phototypes IV-V |
2021 |
Australasian Journal of Dermatology |
62 |
2 |
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e212 |
e216 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092675176&doi=10.1111%2fajd.13486&partnerID=40&md5=a36758f77d6cfee5443e0a9ae6f60fed |
Department of Dermatology and Venereology, Faculty of Medicine Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
Sitohang, I.B.S., Department of Dermatology and Venereology, Faculty of Medicine Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Rahmayunita, G., Department of Dermatology and Venereology, Faculty of Medicine Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Hosfiar, V.A., Department of Dermatology and Venereology, Faculty of Medicine Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Ninditya, S., Department of Dermatology and Venereology, Faculty of Medicine Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Augustin, M., Department of Dermatology and Venereology, Faculty of Medicine Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
The aim of the study is to evaluate the effectiveness of water as a substitute for sodium bicarbonate plus solution in the neutralisation process of chemical peeling using a 35% glycolic acid solution. This randomised, double-blind, split-face, controlled trial was conducted in an outpatient setting. A total of 126 healthy patients with skin phototypes IV–V aged between 18 and 60 years old were recruited. Chemical peeling was performed with 35% glycolic acid solution. One side of the face was neutralised with water, and the other side of the face was neutralised with a sodium bicarbonate plus solution. The main outcome of this study was measured by the degree of erythema, pruritus and pain scores. Significant difference in the pain score immediately after the neutralisation process of the chemical peeling with glycolic acid was seen. However, there were no significant differences in the degree of erythema or the pruritus score. These results indicate that water can be used as a substitute for sodium bicarbonate plus solution in the neutralisation process of chemical peeling with 35% glycolic acid in patients with skin phototypes IV–V. © 2020 The Australasian College of Dermatologists |
chemexfoliation; glycolic acid peel; skin phototypes IV–V; sodium bicarbonate; split face; water |
bicarbonate; glycolic acid; water; bicarbonate; buffer; keratolytic agent; adult; Article; chemexfoliation; clinical effectiveness; clinical protocol; Clinician Erythema Assessment scale; controlled study; cutaneous parameters; disease severity; documentation; double blind procedure; erythema; female; human; human experiment; male; middle aged; normal human; photosensitivity; pruritus; randomized controlled trial; rating scale; scoring system; visual analog scale; young adult; chemexfoliation; procedures; Adult; Buffers; Chemexfoliation; Double-Blind Method; Female; Glycolates; Humans; Keratolytic Agents; Male; Middle Aged; Sodium Bicarbonate; Water; Young Adult |
John Wiley and Sons Inc |
00048380 |
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33070326 |
Article |
Q2 |
670 |
7459 |
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424 |
Satjakoesoemah A.I., Situmorang G.R., Wahyudi I., Rodjani A. |
57189614677;57190001213;36341995300;6504653529; |
Single-stage urethroplasty: An eight-year single-centre experience and its associated factors for urethrocutaneous fistula |
2021 |
Journal of Clinical Urology |
14 |
3 |
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190 |
195 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088243456&doi=10.1177%2f2051415820941746&partnerID=40&md5=9a30aabea7f642db31b4abbbd426894e |
Department of Urology, Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Indonesia |
Satjakoesoemah, A.I., Department of Urology, Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Indonesia; Situmorang, G.R., Department of Urology, Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Indonesia; Wahyudi, I., Department of Urology, Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Indonesia; Rodjani, A., Department of Urology, Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Indonesia |
Introduction: This study aimed to describe single-stage urethroplasty and to determine factors associated with urethrocutaneous fistula after the procedure at our institution. Methods: All hypospadias patients without any prior surgery who underwent single-stage urethroplasty from July 2010 to January 2018 were included. In total, 179 patients were followed for at least one year postoperatively. Information on types of hypospadias, operation techniques, chordee degree, preoperative pyuria (urine white blood cell count >5/HPF), urethral defect length and urethrocutaneous fistula formation was collected. Results: We obtained 103 cases of posterior (57.5%), middle (57 cases) and anterior (19 cases) hypospadias on whom onlay preputial island flap (71 cases), tubularized incised plate (46 cases) and Duckett’s transverse preputial island flap (35 cases) were conducted. After 47.1±25.8 months of follow-up, urethrocutaneous fistula were found in 23 (12.8%) patients associated with posterior hypospadias occurrence (p=0.025), longer urethral defect (p=0.007) and preoperative pyuria (p<0.001). Chordee degree (p=0.886) and age (p=0.187) were not significant factors associated with urethrocutaneous fistula formation. Conclusion: Single-stage urethroplasty is a versatile procedure for various hypospadias cases, with a urethrocutaneous fistula rate of 12.8%. Posterior hypospadias, preoperative pyuria and longer urethral defect were associated with urethrocutaneous fistula formation. Level of evidence: Level 2B. © British Association of Urological Surgeons 2020. |
hypospadias; risk factors; Single stage; single-centre experience; urethrocutaneous fistula; urethroplasty |
cefoperazone; cefotaxime; chloramphenicol; adolescent; adult; Article; child; chordee; cohort analysis; cystostomy; follow up; human; hypospadias; infant; island flap; leukocyturia; major clinical study; male; operation duration; preoperative evaluation; pyuria; renal system parameters; retrospective study; skin fistula; ureter fistula; urethral defect length; urethroplasty; urinary tract infection |
SAGE Publications Ltd |
20514158 |
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Article |
Q3 |
192 |
19868 |
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428 |
Soetisna T.W. |
57214887740; |
A new hope of cd133+ bone marrow stem cell for functional exercise capacity improvement in low ejection fraction coronary artery bypass graft patients: A clinical trial |
2021 |
Bali Medical Journal |
10 |
1 |
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229 |
233 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106002523&doi=10.15562%2fbmj.v10i1.2255&partnerID=40&md5=80046a217774aa6ef82836d9a524c4ff |
Department of Cardiothoracic and Vascular Surgery, National Cardiovascular Center Harapan Kita, Jakarta, Indonesia; Department of Surgery, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Soetisna, T.W., Department of Cardiothoracic and Vascular Surgery, National Cardiovascular Center Harapan Kita, Jakarta, Indonesia, Department of Surgery, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Background: Patients with low ejection fraction who are undergoing coronary artery bypass graft (CABG) only have an insignificant improvement in ejection fraction. This condition will make a little hope to achieve an improvement in physical performance. But now, from a view study, CD133+ stem cells offer new hope for this situation. This study evaluates CD133+ bone marrow stem cells’ role for functional exercise capacity improvement in low ejection fraction coronary artery bypass graft patients. Methods: Thirty patients with ischemic heart disease who had ejection fraction<35% at the National Cardiovascular Center were randomized into 2 groups. The treatment group undergoes the CABG + CD133+ procedure and the control group undergoes the CABG only. All research subjects underwent follow-up before and 6 months after the procedure. Fraction ejection, scar size, wall motion score index, ventricular dimensions, myocardial perfusion measured by cardiac MRI, 6 Minutes Walking Test (6MWT) and Minnesota Living with Heart Failure Questionnaire (MLHFQ) as an additional parameter for physical performance and quality of life. Data was analyzed using SPSS version 21 for Windows. Results: The results of the fraction ejection parameters showed a significant improvement in the treatment group, from 25.88±5.66% to 34.57%±11.31% compared to CABG only 30.18±3.85% to 31.61±7.89% (p=0.040), in the perfusion defect showed improvement but not significant, left ventricular end-systolic volume and left ventricular end-diastolic volume showed improvement with no significant result, scar size was found to be an improvement in the treatment group 10 persons (76.92%) compared to the control group 5 persons (38, 46%) (p=0.040), the wall motion score index and 6MWT showed a significant improvement in the treatment group (p=0.003 and p=0.03, respectively). The MLHFQ parameter showed improvement but not significant. Conclusion: CD 133+ stem cell implantation in patients with low ejection fraction who undergo CABG provides improved myocardial function and indirectly improves functional exercise capacity and patients’ quality of life. © 2021, Sanglah General Hospital. All rights reserved. |
CABG; CD133+ Stem cell; Functional exercise capacity |
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Sanglah General Hospital |
20891180 |
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Article |
#N/A |
#N/A |
#N/A |
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429 |
Ocviyanti D., Putri R.A. |
57189661230;57226166006; |
Biopsychosocial aspect of pregnant women suspected brainstem death [Aspek Biopsikososial pada Perempuan Hamil dengan Kecurigaan Mati Batang Otak] |
2021 |
Indonesian Journal of Obstetrics and Gynecology |
9 |
2 |
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107 |
110 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115419653&doi=10.32771%2finajog.v9i2.1269&partnerID=40&md5=41a14ffbc68b4ced0cd44396ae92afad |
Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia |
Ocviyanti, D., Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Putri, R.A., Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia |
Objective: Diagnosis of brainstem death and the vital organ function support in the pregnant woman to prolong gestation to attain fetal viability is still controversial. The decision is influenced by ethical and legal issue in the country. Another consideration is the hospital cost and health insurance coverage. This article purpose is to report a case and discuss the biopsychosocial aspect of this issue, so the doctors know how to decide a similar case. Methods: We reported a suspected brainstem death in pregnant women and discussed the holistic approach. Case: This case is a-38-year-old women, third pregnancy, 22 weeks of gestation, referred from the secondary hospital in a comatose condition. She was diagnosed with brainstem dysfunction due to intracranial mass and cerebral oedema. She wasn't diagnosed with brainstem death due to the electrolyte imbalance that can cause this condition. We did the multidisciplinary management approach. We decided the termination of pregnancy would only be performed if the fetus reaches 28 weeks of gestational age (with survival rate on perinatology is 31%). From the husband point of view, since the attending doctors have not declared the mother to be dead, then the husband still want to keep the mother in full life support. The patient and the fetus died on the 8th day of hospitalization. The patient was fully paid for by Indonesian Health Insurance. Conclusions: Maternal brainstem dysfunction and brainstem death during pregnancy are rare. In Indonesia, ethical and legal consideration to keep both mother and fetus are appropriate with the general social, cultural, and religious values. However, we recommend managing every single case individually with an intensive multidisciplinary approach due to the possibility of the different personal value of the patient. © Creative Commons Atribuição-Não Comercial 4.0 Internacional |
Brain death; Brainstem dysfunction; Ethic; Fetal; Legal; Pregnancy |
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Indonesian Society of Obstetrics and Gynecology |
23386401 |
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Article |
#N/A |
#N/A |
#N/A |
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430 |
Mariati N.M.A.S., Sugiarto A., Endriani E., Lestari R., Anindita K. |
57223228784;57189612291;57222314378;57223220725;57192903786; |
High flow nasal cannula to prevent intubation in obese patient with COVID-19 induced ARDS: a case report |
2021 |
Anaesthesia, Pain and Intensive Care |
25 |
2 |
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212 |
216 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105281913&doi=10.35975%2fAPIC.V25I2.1473&partnerID=40&md5=2108db369e86e24a12b1ed89d9a0970b |
Department of Anesthesiology & Intensive Care, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Anesthesiology & Intensive Care, Faculty of Medicine, University of Mataram-West Nusa Tenggara General Hospital, Lombok, Indonesia; Pulmonology Department, Faculty of Medicine, University of Mataram-West Nusa Tenggara General Hospital, Lombok, Indonesia; Department of Internal Medicine, Siloam Hospitals Mataram, Jl. Majapahit, Pagesangan, Kec. Mataram, Kota Mataram, Nusa Tenggara Bar, 83115, Indonesia |
Mariati, N.M.A.S., Department of Anesthesiology & Intensive Care, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Sugiarto, A., Department of Anesthesiology & Intensive Care, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Endriani, E., Department of Anesthesiology & Intensive Care, Faculty of Medicine, University of Mataram-West Nusa Tenggara General Hospital, Lombok, Indonesia; Lestari, R., Pulmonology Department, Faculty of Medicine, University of Mataram-West Nusa Tenggara General Hospital, Lombok, Indonesia; Anindita, K., Department of Internal Medicine, Siloam Hospitals Mataram, Jl. Majapahit, Pagesangan, Kec. Mataram, Kota Mataram, Nusa Tenggara Bar, 83115, Indonesia |
Obese patients have been identified to have a poor prognosis and a higher risk of death from coronavirus disease-2019 (COVID-19). We present reports of two obese patients infected with COVID-19 with acute respiratory distress syndrome (ARDS) who showed improvement with high flow nasal cannula (HFNC). HFNC is a noninvasive oxygen delivery device which is capable of delivering a high concentration of oxygen that can reduce the requirement for tracheal intubation. HFNC is convenient to use, can reduce dyspnea without effecting the length of stay in ICU in these cases; and has a high merit to be used in selected patients requiring oxygen before attempting intubation and mechanical ventilation. © the authors. |
ARDS; COVID-19; HFNC; High flow nasal cannula; Obese |
alanine aminotransferase; antiarrhythmic agent; ascorbic acid; aspartate aminotransferase; azithromycin; C reactive protein; chloroquine; dexamethasone; famotidine; heparin; hypertensive factor; levofloxacin; low molecular weight heparin; methylprednisolone; micafungin; oseltamivir; remdesivir; SARS-CoV-2 convalescent plasma; vancomycin; adult; adult respiratory distress syndrome; ageusia; alanine aminotransferase blood level; anosmia; Article; artificial ventilation; aspartate aminotransferase blood level; case report; clinical article; coronavirus disease 2019; coughing; COVID-19 testing; crackle; diarrhea; drug withdrawal; dyspnea; endotracheal intubation; fever; heart arrhythmia; high flow nasal cannula therapy; hospital admission; human; hypotension; hypoxemia; intensive care unit; le |
Faculty of Anaesthesia, Pain and Intensive Care, AFMS |
16078322 |
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Article |
Q3 |
170 |
21404 |
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431 |
Marzuki N.S., Idris F.P., Kartapradja H., Renata S., Harahap A., Latief Batubara J.R. |
37111053300;57224096699;6504373157;57224098534;6507325543;57224097014; |
Accuracy of urinary etiocholanolone/androsterone ratio as alternative to serum testosterone/dihydrotestosterone ratio for diagnosis of 5 alpha-reductase type 2 deficiency patients and carriers in Indonesia |
2021 |
International Journal of Endocrinology and Metabolism |
19 |
2 |
e109510 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106858176&doi=10.5812%2fIJEM.109510&partnerID=40&md5=262ad88d94d397f7bc55e0ace31050be |
Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Department of Child Health, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Marzuki, N.S., Eijkman Institute for Molecular Biology, Jakarta, Indonesia, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Idris, F.P., Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Kartapradja, H., Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Renata, S., Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Harahap, A., Eijkman Institute for Molecular Biology, Jakarta, Indonesia, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Latief Batubara, J.R., Department of Child Health, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Background: The 5 Alpha-reductase type 2 deficiency (5ARD2) is an inherited condition, which clinically presents as variable degrees of under virilization in affected 46,XY individuals. In the diagnostic pathway of 5ARD2, the testosterone/dihydrotestosterone (T/DHT) ratio is broadly employed before molecular analysis of the SRD5A2 gene. However, due to cost-benefit considerations, the DHT test in our country is routinely lacking in clinical settings; therefore, we considered applying the urinary etiocholanolone/androsterone (Et/An) ratio as an alternative test. Objectives: We aimed to determine the diagnostic value of the urinary Et/An ratio versus the T/DHT ratio in diagnosing 5ARD2 patients and carriers. Methods: Sixty-six suspected 5ARD2 46,XY disorders of sex development (DSD) individuals and 95 family members were recruited in the study. Their clinical manifestations, T/DHT and urinary Et/An ratios, and SRD5A2 genes were analyzed. Using molecular analysis of the SRD5A2 gene as the gold standard, we compared the accuracy of both ratios in diagnosing 5ARD2 patients and carriers with receiver operating characteristic (ROC) curve analysis. Results: Thirty-seven patients were confirmed molecularly to have 5ARD2, and the rest (n = 29) were assessed as normal controls, while in the carrier group, 53 were molecularly confirmed as carriers and 42 as controls. The AUCs (areas under the curve) of the T/DHT and urinary Et/An ratios were 57.7% (95% CI 43.0 - 72.4%, P > 0.05) and 79.7% (95% CI 69.0 - 90.4%, P < 0.001), respectively, in diagnosing 5ARD2 patients and 54.1% (95% CI 42.4 - 65.8%, P > 0.05) and 75.1% (95% CI 65.1 - 85.1%, P < 0.001), respectively, in diagnosing carriers. The cutoff value of the urinary Et/An ratio was set at ≥ 0.95 for detecting 5ARD2 patients and ≥ 0.99 for detecting carriers. Conclusions: The testosterone/DHT ratio was inaccurate in diagnosing 5ARD2 patients. When molecular analysis for the SRD5A2 gene is lacking, the urinary Et/An ratio may be a useful test to diagnose 5ARD2 patients and carriers. Copyright © 2021, International Journal of Endocrinology and Metabolism. |
46; 5 Alpha-reductase 2 deficiency; Androsterone; Dihydrotestosterone; Etiocholanolone; SRD5A2; Testosterone; XY DSD |
androstanolone; androsterone; chorionic gonadotropin; etiocholanolone; genomic DNA; steroid; steroid 5alpha reductase 2; testosterone; 5 alpha reductase deficiency; adolescent; adult; analytical parameters; area under the curve; Article; child; clinical article; clinical assessment; clinical feature; competitive ELISA; controlled study; cost benefit analysis; diagnostic accuracy; diagnostic value; disorder of sex development; female; genetic analysis; hormone urine level; human; human tissue; Indonesia; infant; male; molecular biology; neutrophil lymphocyte ratio; newborn; receiver operating characteristic; serum testosterone dihydrotestosterone ratio; testosterone blood level; urinary etiocholanolone androsterone ratio |
Kowsar Medical Institute |
1726913X |
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Article |
Q3 |
652 |
7699 |
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433 |
Jiamsakul A., Azwa I., Zhang F., Yunihastuti E., Ditangco R., Kumarasamy N., Ng O.T., Chan Y.-J., Ly P.S., Choi J.Y., Lee M.-P., Pujari S., Kiertiburanakul S., Chaiwarith R., Merati T.P., Sangle S., Khusuwan S., Sim B.L.H., Avihingsanon A., Do C.D., Tanuma J., Ross J., Law M., the TREAT Asia HIV Observational Database of IeDEA Asia-Pacific |
55285745500;55553159100;55503803800;57221273925;55406840800;7003549856;57203665233;33667461800;9743902800;48761023600;56143671100;57205894660;6506539792;57203665049;57203678680;6602877716;56166613100;9242778900;57200282477;56658396600;57208428839;57193109926;57222965808; |
Treatment modification after second-line failure among people living with HIV in Asia-Pacific |
2021 |
Antiviral Therapy |
25 |
7 |
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377 |
387 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106544855&doi=10.3851%2fIMP3388&partnerID=40&md5=485e6ef464b90e2346135f1d92ba9394 |
The Kirby Institute, UNSW Sydney, Sydney, NSW, Australia; University of Malaya Medical Centre, Kuala Lumpur, Malaysia; Beijing Ditan Hospital, Capital Medical University, Beijing, China; Working Group on AIDS, Faculty of Medicine, University of Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Research Institute for Tropical Medicine, Manila, Philippines; Chennai Antiviral Research and Treatment Clinical Research Site (CART CRS), The Voluntary Health Services (VHS), Chennai, India; Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore; Taipei Veterans General Hospital, Taipei, Taiwan; National Center for HIV/AIDS, Dermatology and STDs, University of Health Sciences, Phnom Penh, Cambodia; Division of Infectious Diseases, Department of Internal Medicine, Yonsei University, College of Medicine, Seoul, South Korea; Queen Elizabeth Hospital, Yau Ma Tei, Hong Kong; Institute of Infectious Diseases, Pune, India; Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand; Research Institute for Health Sciences, Chiang Mai, Thailand; Faculty of Medicine, Udayana University, Sanglah Hospital, Bali, Indonesia; BJ Government Medical College, Sassoon General Hospital, Pune, India; Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand; Hospital Sungai Buloh, Sungai Buloh, Malaysia; HIV-NAT/Thai Red Cross AIDS Research Centre, Bangkok, Thailand; Bach Mai Hospital, Hanoi, Viet Nam; National Center for Global Health and Medicine, Tokyo, Japan; TREAT Asia, AmfAR, The Foundation for AIDS Research, Bangkok, Thailand |
Jiamsakul, A., The Kirby Institute, UNSW Sydney, Sydney, NSW, Australia; Azwa, I., University of Malaya Medical Centre, Kuala Lumpur, Malaysia; Zhang, F., Beijing Ditan Hospital, Capital Medical University, Beijing, China; Yunihastuti, E., Working Group on AIDS, Faculty of Medicine, University of Indonesia/Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Ditangco, R., Research Institute for Tropical Medicine, Manila, Philippines; Kumarasamy, N., Chennai Antiviral Research and Treatment Clinical Research Site (CART CRS), The Voluntary Health Services (VHS), Chennai, India; Ng, O.T., Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore; Chan, Y.-J., Taipei Veterans General Hospital, Taipei, Taiwan; Ly, P.S., National Center for HIV/AIDS, Dermatology and STDs, University of Health Sciences, Phnom Penh, Cambodia; Choi, J.Y., Division of Infectious Diseases, Department of Internal Medicine, Yonsei University, College of Medicine, Seoul, South Korea; Lee, M.-P., Queen Elizabeth Hospital, Yau Ma Tei, Hong Kong; Pujari, S., Institute of Infectious Diseases, Pune, India; Kiertiburanakul, S., Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand; Chaiwarith, R., Research Institute for Health Sciences, Chiang Mai, Thailand; Merati, T.P., Faculty of Medicine, Udayana University, Sanglah Hospital, Bali, Indonesia; Sangle, S., BJ Government Medical College, Sassoon General Hospital, Pune, India; Khusuwan, S., Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand; Sim, B.L.H., Hospital Sungai Buloh, Sungai Buloh, Malaysia; Avihingsanon, A., HIV-NAT/Thai Red Cross AIDS Research Centre, Bangkok, Thailand; Do, C.D., Bach Mai Hospital, Hanoi, Viet Nam; Tanuma, J., National Center for Global Health and Medicine, Tokyo, Japan; Ross, J., TREAT Asia, AmfAR, The Foundation for AIDS Research, Bangkok, Thailand; Law, M., The Kirby Institute, UNSW Sydney, Sydney, NSW, Australia; the TREAT Asia HIV Observational Database of IeDEA Asia-Pacific |
Background: The World Health Organization recommends continuation with the failing second-line regimen if third-line option is not available. We investigated treatment outcomes among people living with HIV in Asia who continued with failing second-line regimens compared with those who had treatment modifications after failure. Methods: Treatment modification was defined as a change of two antiretrovirals, a drug class change or treatment interruption (TI), all for >14 days. We assessed factors associated with CD4 changes and undetectable viral load (UVL <1,000 copies/ml) at 1 year after second-line failure using linear and logistic regression, respectively. Survival time was analysed using competing risk regression. Results: Of the 328 patients who failed second-line ART in our cohorts, 208 (63%) had a subsequent treatment modification. Compared with those who continued the failing regimen, the average CD4 cell increase was higher in patients who had a modification without TI (difference =77.5, 95% CI 35.3, 119.7) while no difference was observed among those with TI (difference =-5.3, 95% CI -67.3, 56.8). Compared with those who continued the failing regimen, the odds of achieving UVL was lower in patients with TI (OR=0.18, 95% CI 0.06, 0.60) and similar among those who had a modification without TI (OR=1.97, 95% CI 0.95, 4.10), with proportions of UVL 60%, 22% and 75%, respectively. Survival time was not affected by treatment modifications. Conclusions: CD4 cell improvements were observed in those who had treatment modification without TI compared with those on the failing regimen. When no other options are available, maintaining the same failing ART combination provided better VL control than interrupting treatment. © 2020 International Medical Press |
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Human immunodeficiency virus proteinase inhibitor; nonnucleoside reverse transcriptase inhibitor; RNA directed DNA polymerase inhibitor; adult; antiretroviral therapy; Article; Asia; CD4 lymphocyte count; clinical outcome; cohort analysis; confidence interval; controlled study; female; human; Human immunodeficiency virus infected patient; Human immunodeficiency virus infection; linear regression analysis; logistic regression analysis; major clinical study; male; odds ratio; survival time; treatment duration; treatment modification; undetectable virus load; virus load |
International Medical Press Ltd |
13596535 |
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33843656 |
Article |
Q2 |
747 |
6553 |
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434 |
Pustimbara A., Putri D.C., Prakoso N.M., Priambodo R., Ariani Y., Yuliarti K., Bowolaksono A., Sjarif D.R. |
57217086984;57204606877;57214084050;57190937999;57200504713;54917483500;57205093224;6506242684; |
Novel base alterations at intron 3 of 6-pyruvoyl-tetrahydropterin synthase gene in Indonesian population |
2021 |
AIP Conference Proceedings |
2331 |
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050028 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103860378&doi=10.1063%2f5.0042047&partnerID=40&md5=80d1cbd9c9334c733fed5caccbb5bdad |
Human Genetics Research Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, 10430, Indonesia; Department of Pediatric, Universitas Indonesia, RSUPN, Dr. Cipto Mangunkusumo, Jakarta, 10430, Indonesia; Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia |
Pustimbara, A., Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia; Putri, D.C., Human Genetics Research Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, 10430, Indonesia, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia; Prakoso, N.M., Human Genetics Research Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, 10430, Indonesia; Priambodo, R., Human Genetics Research Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, 10430, Indonesia; Ariani, Y., Human Genetics Research Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, 10430, Indonesia, Department of Pediatric, Universitas Indonesia, RSUPN, Dr. Cipto Mangunkusumo, Jakarta, 10430, Indonesia, Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Yuliarti, K., Human Genetics Research Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, 10430, Indonesia, Department of Pediatric, Universitas Indonesia, RSUPN, Dr. Cipto Mangunkusumo, Jakarta, 10430, Indonesia; Bowolaksono, A., Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia; Sjarif, D.R., Human Genetics Research Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, 10430, Indonesia, Department of Pediatric, Universitas Indonesia, RSUPN, Dr. Cipto Mangunkusumo, Jakarta, 10430, Indonesia |
6-pyruvoyl-tetrahydropterin synthase (PTPS) or tetrahydrobiopterin (BH4) deficiency is the most common enzyme synthesis defect which was reported to cause of hyperphenylalaninemia. This deficiency is caused by pathogenic variant in exons and introns of 6-pyruvoyl-tetrahydropterin synthase (PTS) gene in chromosome 11q22.3-q23.3. This study is focused on the detection of DNA variants in intron 3 especially for insertion and base alteration. Methods that has been carried out in this study are DNA isolation, polymerase chain reaction (PCR), the visualization of PCR products through DNA electrophoresis, and Sanger sequencing. A total 29 variants have been characterized in this study, obtained from the DNA of one Indonesian PTPS patients and 33 healthy individuals as control. Those alterations were categorized into substitution and intronic insertion and located in the sequence of intron 3 and 4 of PTS gene. Further analyses are required to be performed to characterize the effect of identified variants to the splicing events of PTS mRNA. © 2021 Author(s). |
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American Institute of Physics Inc. |
0094243X |
9780735440753 |
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Conference Paper |
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177 |
20880 |
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