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798 |
Rinaldi I., Hamonangan R., Azizi M.S., Cahyanur R., Wirawan F., Fatya A.I., Budiananti A., Winston K. |
23475122400;57194734003;57209578916;37066991400;57205313463;57224164794;57224171532;57224165400; |
Diagnostic value of neutrophil lymphocyte ratio and D-Dimer as biological markers of deep vein thrombosis in patients presenting with unilateral limb edema |
2021 |
Journal of Blood Medicine |
12 |
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313 |
325 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107134090&doi=10.2147%2fJBM.S291226&partnerID=40&md5=e8ea48e2b9ed2ba98d1bdc094dbbe4f7 |
Division of Hematology and Medical Oncology, Department of Internal Medicine, Cipto Mangunkusumo National General Hospital Faculty of Medicine Universitas Indonesia, Jakarta, 10430, Indonesia; Department of Internal Medicine, PGI Cikini Hospital, Jakarta, Indonesia; Division of Cardiology, Department of Internal Medicine, Cipto Mangunkusumo National General Hospital Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Rinaldi, I., Division of Hematology and Medical Oncology, Department of Internal Medicine, Cipto Mangunkusumo National General Hospital Faculty of Medicine Universitas Indonesia, Jakarta, 10430, Indonesia; Hamonangan, R., Department of Internal Medicine, PGI Cikini Hospital, Jakarta, Indonesia; Azizi, M.S., Division of Cardiology, Department of Internal Medicine, Cipto Mangunkusumo National General Hospital Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Cahyanur, R., Division of Hematology and Medical Oncology, Department of Internal Medicine, Cipto Mangunkusumo National General Hospital Faculty of Medicine Universitas Indonesia, Jakarta, 10430, Indonesia; Wirawan, F., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Fatya, A.I., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Budiananti, A., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Winston, K., Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Introduction: Patients with deep vein thrombosis (DVT) pose high morbidity and mortality risk thus needing fast and accurate diagnosis. Wells clinical prediction scores with D-dimer testing are traditionally used to rule out patients with low probability of DVT. However, D-dimer testing has a few limitations regarding its relatively low specificity. Neutrophil-lymphocyte ratio (NLR), a marker of inflammation, was found to increase in DVT. Hence, we aimed to evaluate the role of NLR for DVT diagnosis. Methods: Data were collected from medical records of patients with suspected DVT at Cipto Mangunkusumo National General Hospital during January-December 2014. Diagnosis of DVT was conducted using lower limb ultrasonography. Diagnostic values for NLR, D-dimer, and NLR + D-dimer were determined by receiver operating characteristic (ROC) analysis to obtain area under the curve (AUC), sensitivity, specificity, negative predictive value, and positive predictive values. Sensitivity and specificity analyses of NLR and D-dimer were also conducted based on Wells score and divided into groups of low and high probability of DVT. Results: The AUC values for NLR, D-dimer, and NLR + D-dimer were 72.6%, 70.4%, and 76.1%, respectively. The optimal cut-off value determined for NLR was 5.12 with sensitivity of 67.7%, specificity of 67.9%, PPV of 68.85%, and NPV of 64.91% in differentiating subjects with and without DVT. This study also found that D-dimer had sensitivity of 69.4%, specificity of 71.4%, PPV of 72.88%, and NPV of 67.8%. Meanwhile, the NLR + D-dimer combination had sensitivity of 66.1% and specificity of 72.6%. Multivariate analysis showed that NLR (OR: 2.636; 95% CI: 1.144-6.076; p: 0.023) and D-dimer (OR: 4.175; 95% CI: 1.810-9.633; p: 0.001) were associated with DVT. Conclusion: NLR value has wider AUC than D-Dimer and is relatively easier to obtain and does not require specific assay, thus enabling rapid evaluation of symptomatic patients suspected of having DVT. Adding NLR to D-dimer increased AUC to detect DVT. Therefore, NLR could serve as a complementary diagnostic tool for D-dimer to exclude DVT, especially in low clinical probability patients. © 2021 Rinaldi et al. |
D-dimer; Deep vein thrombosis; Inflammation; Neutrophils lymphocyte ratio; NLR |
biological marker; C reactive protein; D dimer; hemoglobin; adult; Article; atrial fibrillation; cerebrovascular disease; chronic kidney failure; controlled study; deep vein thrombosis; diabetes mellitus; diagnostic test accuracy study; echography; extracellular trap; female; health care facility; heart disease; hematological parameters; human; hypertension; inflammation; internal medicine; lymphocyte count; major clinical study; male; middle aged; nanotechnology; neutrophil lymphocyte ratio; peripheral edema; predictive value; pregnancy; receiver operating characteristic; sensitivity analysis; sensitivity and specificity; venous thromboembolism |
Dove Medical Press Ltd |
11792736 |
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Article |
Q3 |
676 |
7401 |
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799 |
Atmakusuma T.D., Lubis A.M. |
57216961785;56702707800; |
Correlation of serum ferritin and liver iron concentration with transient liver elastography in adult thalassemia intermedia patients with blood transfusion |
2021 |
Journal of Blood Medicine |
12 |
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235 |
243 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107032322&doi=10.2147%2fJBM.S303703&partnerID=40&md5=408d9c4b4409a7ccccfcbc0f6dca1380 |
Division of Hematology-Medical Oncology, Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia |
Atmakusuma, T.D., Division of Hematology-Medical Oncology, Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Lubis, A.M., Division of Hematology-Medical Oncology, Department of Internal Medicine, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia |
Introduction: Iron overload is a common feature of thalassemia intermedia due to regular blood transfusion and increased gastrointestinal iron absorption. Early detection and adequate iron chelator can decrease morbidity and mortality from iron overload. Liver iron concentration (LIC) by MRI T2* is the best non-invasive way to measure body iron stores. However, this method is expensive and not available nationwide in Indonesia. The aim of this study was to identify liver iron overload and correlation of transferrin saturation, serum ferritin, liver MRI T2* and LIC with transient liver elastography in adult thalassemia intermedia patients. Methods: This is a cross-sectional study of 45 patients with thalassemia intermedia with blood transfusion and with and without iron chelator therapy. The study was conducted at Cipto Mangunkusumo Hospital from August through October 2016. We performed measurements of transferrin saturation, serum ferritin level, transient liver elastography and liver MRI T2*. Pearson and Spearman correlation tests were used to evaluate the correlation between transient liver elastography with transferrin saturation, serum ferritin, liver MRI T2*and LIC. Results and Discussion: This study showed that 64.4% of study subjects are β-Hb E thalassemia intermedia. Furthermore, 84.4% of study subjects have regular transfusion. Based on liver MRI T2*all subjects suffered from liver iron overload, 48.9% had severe degree. Median value of liver MRI T2* was 1.6 ms. Mean serum ferritin was 2831 ng/mL, with median transferrin saturation of 66%. Mean of LIC corresponding to liver MRI T2* and mean liver stiffness measurement was 15.36±7.37 mg Fe/gr dry weight and 7.7±3.8 kPa, respectively. Liver stiffness correlated with serum ferritin (r=0.651; p=0.000), liver MRI T2* (r=−0.357; p=0.016), and LIC (r=0.433; p=0.003). No correlation was found between liver elastography and transferrin saturation (r=0.204; p=0.178). Conclusion: Serum ferritin, liver MRI T2*and LIC correlated with liver elastography. No correlation was found between transferrin saturation and liver elastography. © 2021 Atmakusuma and Lubis. |
LIC; Liver MRI T2*; Serum ferritin; Thalassemia intermedia; Transient liver elastography |
alanine aminotransferase; aspartate aminotransferase; bilirubin; C reactive protein; deferasirox; deferiprone; hemoglobin; hemoglobin F; iron; iron chelating agent; adult; alpha thalassemia; Article; beta thalassemia; blood sampling; blood transfusion; capillary electrophoresis; clinical article; concentration (parameter); correlation analysis; cross-sectional study; DNA determination; elastography; female; ferritin blood level; hemoglobin E-beta thalassemia; high performance liquid chromatography; human; hypertransaminasemia; iron overload; liver examination; liver function test; liver hemosiderosis; liver stiffness; male; nuclear magnetic resonance imaging; splenectomy; thalassemia intermedia; transferrin saturation |
Dove Medical Press Ltd |
11792736 |
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Article |
Q3 |
676 |
7401 |
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800 |
Harbuwono D.S., Mokoagow M.I., Magfira N., Helda H. |
36056341600;57216673213;57221224711;57195467249; |
ADA Diabetes Risk Test Adaptation in Indonesian Adult Populations: Can It Replace Random Blood Glucose Screening Test? |
2021 |
Journal of Primary Care and Community Health |
12 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106969426&doi=10.1177%2f21501327211021015&partnerID=40&md5=cfd7fedfd14bbf80f4f0f6c421b6a755 |
Faculty of Medicine, Universitas Indonesia, Central Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Fatmawati General Hospital, Jakarta, Indonesia; Faculty of Public Health, Universitas Indonesia, Jakarta, Indonesia |
Harbuwono, D.S., Faculty of Medicine, Universitas Indonesia, Central Jakarta, Indonesia, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Mokoagow, M.I., Faculty of Medicine, Universitas Indonesia, Central Jakarta, Indonesia, Fatmawati General Hospital, Jakarta, Indonesia; Magfira, N., Faculty of Public Health, Universitas Indonesia, Jakarta, Indonesia; Helda, H., Faculty of Public Health, Universitas Indonesia, Jakarta, Indonesia |
Aims: The use of non-invasive and easily available assessment tools such as the ADA diabetes risk test is recommended for diabetes screening among general population. This study aims to assess the validity of the ADA diabetes risk test in screening for screening hyperglycemia in Indonesian population. Methods: This cross-sectional study conducted at primary health care in Cibeber sub district at Cilegon city, Banten province, Indonesia. Subjects were aged ≥45 years old without a prior diagnosis of diabetes were recruited consecutively. The risk of hyperglycemia was measured using the ADA Diabetes Risk Test. Random capillary blood glucose (RcBG) with a cut-off value >140 mg/dL used as a comparison. Results: From a total of 134 subjects, 23.13% of subjects (n = 31) had hyperglycemia. The ADA Diabetes Risk Test gave an area under the ROC curve (AUC) of 0.71 (95% CI: 0.60-0.81) with an optimal cut-off of value ≥5. The sensitivity of the ADA diabetes risk test in diagnosing hyperglycemia was 68% with a false-negative rate (FNR) of 32.26%. Meanwhile, at a cut-off value ≥4, the sensitivity of the ADA Diabetes Risk Test was 93% with an FNR of 9.7%. Conclusion: ADA diabetes risk test provides a valuable result as a diabetes screening tool in the Indonesian population, thus promotes intervention strategies for population known to be at risk © The Author(s) 2021. |
ADA diabetes risk test; adult population; hyperglycemia; Indonesia; screening |
adult; cross-sectional study; diabetes mellitus; glucose blood level; human; Indonesia; mass screening; middle aged; sensitivity and specificity; Adult; Blood Glucose; Cross-Sectional Studies; Diabetes Mellitus; Humans; Indonesia; Mass Screening; Middle Aged; Sensitivity and Specificity |
SAGE Publications Inc. |
21501319 |
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34053367 |
Article |
Q2 |
550 |
9179 |
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801 |
Siagian S.N., Haas N.A., Prakoso R. |
57214134720;7103216848;57192893243; |
Transcatheter pulmonary balloon valvuloplasty of severe valvar pulmonary stenosis and atrial septal defect in patient with severe cyanosis and very low ventricle ejection fractions: a bailout procedure |
2021 |
Cardiology in the Young |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106665812&doi=10.1017%2fS1047951121001967&partnerID=40&md5=92e3509fdca8f306fe3ccb03a7fd028a |
Department of Cardiology and Vascular Medicine, Faculty of Medicine Universitas Indonesia, National Cardiovascular Center Harapan Kita, Jakarta, Indonesia; Department Pediatric Cardiology and Pediatric Intensive Care, Ludwig Maximilians University, University Hospital, Muenchen, Germany |
Siagian, S.N., Department of Cardiology and Vascular Medicine, Faculty of Medicine Universitas Indonesia, National Cardiovascular Center Harapan Kita, Jakarta, Indonesia; Haas, N.A., Department Pediatric Cardiology and Pediatric Intensive Care, Ludwig Maximilians University, University Hospital, Muenchen, Germany; Prakoso, R., Department of Cardiology and Vascular Medicine, Faculty of Medicine Universitas Indonesia, National Cardiovascular Center Harapan Kita, Jakarta, Indonesia |
Most cases of severe or critical pulmonary stenosis are detected early and interventional management is routine within the first days of life. We present a case of a thirteen-year-old boy diagnosed with pulmonary stenosis and atrial septal defect with low ventricle ejection fraction. The patient underwent staged pulmonary balloon valvuloplasty and interventional atrial septal defect closure with good results. © 2021 The Author(s),. Published by Cambridge University Press. |
atrial septal defect; bailout; cyanosis; low ejection fraction; pulmonary balloon valvuloplasty; Pulmonary stenosis |
adolescent; case report; complication; cyanosis; heart septum defect; human; male; pulmonary valve stenosis; transluminal valvuloplasty; Adolescent; Balloon Valvuloplasty; Cyanosis; Heart Septal Defects, Atrial; Humans; Male; Pulmonary Valve Stenosis |
Cambridge University Press |
10479511 |
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34016212 |
Article |
Q3 |
386 |
12454 |
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802 |
Lilihata G., Saputra C., Yaniarti D., Soerarso R. |
57223977510;57223988357;57223991240;57192913673; |
Hyperthyroidism in severe mitral regurgitation post mechanical mitral valve replacement: The effect on warfarin anticoagulation |
2021 |
Cardiovascular Endocrinology and Metabolism |
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146 |
148 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106612745&doi=10.1097%2fXCE.0000000000000233&partnerID=40&md5=01f9076c604667b3b97acb9eb9581c09 |
Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Indonesia, Indonesia; Division of Clinical Cardiology, Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Indonesia, National Cardiovascular Center, Harapan Kita, Jakarta, Indonesia |
Lilihata, G., Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Indonesia, Indonesia; Saputra, C., Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Indonesia, Indonesia; Yaniarti, D., Division of Clinical Cardiology, Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Indonesia, National Cardiovascular Center, Harapan Kita, Jakarta, Indonesia; Soerarso, R., Division of Clinical Cardiology, Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Indonesia, National Cardiovascular Center, Harapan Kita, Jakarta, Indonesia |
A 24-year-old male patient came to the emergency room with melena, gum bleeding and nosebleeds. This patient has a history of mechanical prosthetic mitral valve replacement for severe mitral regurgitation (MR) and consumed warfarin irregularly, but did not come back for regular check-up. Investigations showed greatly increased thyroid function and international normalised ratio (INR) was 15.8. Patients were diagnosed with thyroid storm and bleeding due to prolongation of INR. His hyperthyroid state might have caused increased rate of degradation of vitamin K-dependent clotting factor thereby increased sensitivity to warfarin. Concomitant acute decompensated heart failure, thrombocytopenia and hypoalbuminemia also contributed to his risk of bleeding. Treatment included anti-thyroid therapy as well as warfarin reversal therapy by stopping warfarin, low-dose intravenous vitamin K due to his mechanical prosthetic valve and fresh frozen plasma. In conclusion, hyperthyroidism could increase the response to warfarin so close monitoring is needed to balance the risk of bleeding and thromboembolism. © 2021 Lippincott Williams and Wilkins. All rights reserved. |
anticoagulation; bleeding; hyperthyroidism; mechanical valve; prosthetic valve; warfarin |
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Lippincott Williams and Wilkins |
25740954 |
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Article |
Q3 |
498 |
10107 |
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803 |
Nova R., Nurmaini S., Partan R.U., Putra S.T. |
57210234221;26639610000;57190664693;6603587929; |
Automated image segmentation for cardiac septal defects based on contour region with convolutional neural networks: A preliminary study |
2021 |
Informatics in Medicine Unlocked |
24 |
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100601 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106348104&doi=10.1016%2fj.imu.2021.100601&partnerID=40&md5=37e5f3bcab0ef015f7441efa32757710 |
Department of Child Health, Division of Pediatric Cardiology, Dr. Moh Hoesin Hospital, Faculty of Medicine, Universitas Sriwijaya, Palembang, 30126, Indonesia; Intelligent System Research Group, Universitas Sriwijaya, Palembang, 30139, Indonesia; Department of Medicine, Dr. MohHoesin Hospital, Faculty of Medicine, Universitas Sriwijaya, Palembang, 30126, Indonesia; Department of Child Health, Division of Pediatric Cardiology, Dr. CiptoMangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia |
Nova, R., Department of Child Health, Division of Pediatric Cardiology, Dr. Moh Hoesin Hospital, Faculty of Medicine, Universitas Sriwijaya, Palembang, 30126, Indonesia; Nurmaini, S., Intelligent System Research Group, Universitas Sriwijaya, Palembang, 30139, Indonesia; Partan, R.U., Department of Medicine, Dr. MohHoesin Hospital, Faculty of Medicine, Universitas Sriwijaya, Palembang, 30126, Indonesia; Putra, S.T., Department of Child Health, Division of Pediatric Cardiology, Dr. CiptoMangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia |
Echocardiogram examination is important for diagnosing cardiac septal defects. With the development of AI-based technology, an echocardiogram examination previously performed manually by cardiologists can be done automatically. Automatic segmentation of cardiac septal defects can help a physician to make an initial diagnosis before referring a pediatric cardiologist for further treatment. In previous studies, automatic object segmentation using convolutional neural networks (CNNs) was one of the DL applications that have been developed for cardiac abnormalities. In this study, we propose a CNN-based U-Net architecture to automatically segment the cardiac chamber to detect abnormalities (holes) in the heart septum. In this study, echocardiogram examinations were performed on atrial septal defects (ASDs), ventricular septal defects (VSDs), atrioventricular septal defects (AVSDs), and normal hearts with patients undergoing echocardiogram examination at Moh Hoesin Hospital in Palembang. The results show that even for the relatively small number of datasets, the proposed technique can produce superior performance in the detection of the cardiac septal defects. Using the proposed segmentation model for four classes produces a pixel accuracy of 99.15%, mean intersection over union (IoU) of 94.69%, mean accuracy of 97.73%, sensitivity of 96.02%, and F1 score of 94.88%, respectively. The plots of the loss and accuracy curve show that all the errors were small, with accuracy rates reaching 99.05%, 98.62%, 99.39%, and 98.97% for ASD, VSD, AVSD, and normal heart, respectively. The comparison accuracy of contour prediction for U-Net was 99.01%, while V-Net was 93.70%. This shows that the U-Net has better accuracy than the V-Net model architecture. It can be proven that the architecture of CNNs has been successful in segmenting the cardiac chamber to detect defects in the heart septum and support the work of cardiologists. © 2021 The Authors |
Cardiac septal defect; CNNs; Contour segmentation; Deep learning |
adult; Article; atrioventricular septal defect; child; clinical article; clinical examination; computer assisted diagnosis; controlled study; convolutional neural network; deconvolution; deep learning; diagnostic accuracy; diagnostic test accuracy study; heart atrium septum defect; heart ventricle septum defect; human; image processing; image segmentation; pediatric patient; two dimensional echocardiography |
Elsevier Ltd |
23529148 |
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Article |
Q3 |
440 |
11223 |
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804 |
Wicaksana P., Kurniati N., Pardede S. |
57193446457;36473260300;57210394278; |
Dyslipidemia among HIV-positive children receiving antiretroviral therapy in Indonesia |
2021 |
HIV and AIDS Review |
20 |
1 |
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46 |
51 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106294127&doi=10.5114%2fhivar.2021.105086&partnerID=40&md5=1f2028e2d19b8667b0fe6419e86d0dfd |
Department of Child Health, Universitas Indonesia, Cipto Mangunkusumo General Hospital, Indonesia |
Wicaksana, P., Department of Child Health, Universitas Indonesia, Cipto Mangunkusumo General Hospital, Indonesia; Kurniati, N., Department of Child Health, Universitas Indonesia, Cipto Mangunkusumo General Hospital, Indonesia; Pardede, S., Department of Child Health, Universitas Indonesia, Cipto Mangunkusumo General Hospital, Indonesia |
Introduction: Antiretroviral (ARV) therapy has changed the status of human immunodeficiency virus (HIV) infection from a high-mortality disease into a chronic one. One of the consequences of long-term use of ARV medications is dyslipidemia, which may progress to cardiovascular disease in the future. The aim of the study was to measure the rate of dyslipidemia among HIV-infected children receiving ARV therapy and related risk factors. Material and methods: A cross-sectional study was conducted at pediatric outpatient clinic, Cipto Mangunkusumo Hospital, Jakarta, Indonesia, from January to July 2019. Lipid profile was examined on 96 eligible subjects and data regarding nutritional status, clinical stage at diagnosis, latest immunosuppression status, latest viral load (VL) value, and latest ARV combination used were obtained from medical records. Bivariate and multivariate analysis were performed to find the association between dependent and independent variables using SPSS version 22. Results: Of 96 subjects included, 52 (54.2%) subjects experienced dyslipidemia. The prevalence of dyslipidemia among those with second-line (containing protease inhibitors) and first-line (containing non-nucleoside reverse transcriptase inhibitors) ARV therapy were 80% and 39%, respectively. The use of second-line ARV therapy was associated with 6.3 times (p < 0.01; 95% CI: 2.4-17.1) increased risk of dyslipidemia compared to first-line ARV therapy. Conclusions: Prevalence of dyslipidemia among HIV-positive children on ARV was high with second-line ARV therapy being a risk factor. © 2021 Termedia Publishing House Ltd.. All rights reserved. |
Children; Dyslipidemia; HAART; HIV; Protease inhibitors |
abacavir; antiretrovirus agent; cholesterol; efavirenz; emtricitabine; high density lipoprotein; lamivudine; lopinavir plus ritonavir; low density lipoprotein; nevirapine; tenofovir; triacylglycerol; zidovudine; adolescent; Article; child; controlled study; cross-sectional study; dyslipidemia; female; highly active antiretroviral therapy; human; Human immunodeficiency virus infected patient; Human immunodeficiency virus infection; hypertriglyceridemia; immune deficiency; Indonesia; lipid fingerprinting; major clinical study; male; nutritional status; pediatric hospital; preschool child; prevalence; school child; virus load |
Termedia Publishing House Ltd. |
17301270 |
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Article |
Q4 |
156 |
22602 |
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806 |
Adriztina I., Munir D., Sandra F., Ichwan M., Bashiruddin J., Putra I.B., Farhat, Sembiring R.J., Sartika C.R., Chouw A., Pratiwi E.D. |
56928394300;57039203600;56016164200;26040779000;56469338300;57194636376;57194721197;57038845000;57205297509;56431084200;57223858156; |
Differentiation capacity of dental pulp stem cell into inner ear hair cell using an in vitro assay: a preliminary step toward treating sensorineural hearing loss |
2021 |
European Archives of Oto-Rhino-Laryngology |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106239040&doi=10.1007%2fs00405-021-06864-9&partnerID=40&md5=5a4ff939b0fff7361593d6d043015bd8 |
Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Jl. Dr. Mansyur No 5, Medan, Sumatera Utara 20155, Indonesia; Division of Oral Biology, Departement of Biochemistry and Molecular Biology, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia; Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Department of Dermatovenerology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Department of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Prodia Stemcell Indonesia, Jl. Kramat VII No.11, Jakarta, Indonesia |
Adriztina, I., Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Jl. Dr. Mansyur No 5, Medan, Sumatera Utara 20155, Indonesia; Munir, D., Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Jl. Dr. Mansyur No 5, Medan, Sumatera Utara 20155, Indonesia; Sandra, F., Division of Oral Biology, Departement of Biochemistry and Molecular Biology, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia; Ichwan, M., Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Bashiruddin, J., Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Putra, I.B., Department of Dermatovenerology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Farhat, Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Jl. Dr. Mansyur No 5, Medan, Sumatera Utara 20155, Indonesia; Sembiring, R.J., Department of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Sartika, C.R., Prodia Stemcell Indonesia, Jl. Kramat VII No.11, Jakarta, Indonesia; Chouw, A., Prodia Stemcell Indonesia, Jl. Kramat VII No.11, Jakarta, Indonesia; Pratiwi, E.D., Prodia Stemcell Indonesia, Jl. Kramat VII No.11, Jakarta, Indonesia |
Purpose: Sensorineural hearing loss (SNHL) is commonly caused by the death or dysfunction of cochlear cell types as a result of their lack of regenerative capacity. However, regenerative medicine, such as stem cell therapy, has become a promising tool to cure many diseases, including hearing loss. In this study, we determined whether DPSCs could differentiate into cochlear hair cell in vitro. Methods: DPSCs derived from human third molar dental pulp were induced into NSCs using a medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) for 7 days, and then into cochlear hair cell using a medium containing EGF and IGF-1 for the next 14 days. We used the neuroepithelial protein marker nestin and cochlear hair cell marker myosin VIIa as the markers for cells differentiation. Cells expressing the positive markers under the microscope were confirmed to have differentiated into cochlear hair cell. Results: DPSCs were successfully induced to differentiate into NSCs, with mean 24% nestin-positive cells. We found that DPSC-derived NSCs have a great capacity in differentiating into inner ear hair cell-like cells with an average of 81% cells presenting myosin VIIa. Thus, DPSCs have high potential to serve as a good resource for SNHL treatment. Conclusion: We found the high potential of DPSCs to differentiate into NSC. The ability of DPSCs in differentiating into neural lineage cell made them a good candidate for regenerative therapy in neural diseases, such as SNHL © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. |
Cochlear hair cell; Dental pulp stem cells; Sensorineural hearing loss |
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Springer Science and Business Media Deutschland GmbH |
09374477 |
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34008035 |
Article |
Q1 |
849 |
5560 |
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807 |
Jusman S.W.A., Azzizah I.N., Sadikin M., Hardiany N.S. |
36518792100;57223677370;57205093115;57192910605; |
Is the mitochondrial function of keloid fibroblasts affected by cytoglobin? |
2021 |
Malaysian Journal of Medical Sciences |
28 |
2 |
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39 |
47 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106010069&doi=10.21315%2fmjms2021.28.2.4&partnerID=40&md5=8c4df0a7d2cdcd87611b189bf2440f21 |
Department of Biochemistry & Molecular Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Faculty of Medicine Universitas Indonesia, Indonesia; Center of Hypoxia & Oxidative Stress Studies, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia |
Jusman, S.W.A., Department of Biochemistry & Molecular Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia, Center of Hypoxia & Oxidative Stress Studies, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Azzizah, I.N., Faculty of Medicine Universitas Indonesia, Indonesia; Sadikin, M., Department of Biochemistry & Molecular Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia, Center of Hypoxia & Oxidative Stress Studies, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Hardiany, N.S., Department of Biochemistry & Molecular Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia, Center of Hypoxia & Oxidative Stress Studies, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia |
Background: A keloid is a benign skin tumour characterised by excessive proliferation of fibroblasts, a process that requires a sufficient amount of energy. The energy needs are associated with adequate oxygen (O2) flow and well-functioning mitochondria. It is known that cytoglobin (CYGB) has a function in O2 distribution. The aim of the present study was to explore whether the inhibition of CYGB expression caused impaired mitochondrial function of keloid fibroblasts. Methods: An in vitro study was conducted on a keloid fibroblast derived from our previous study. The study was carried out in the laboratory of the Biochemistry & Molecular Biology Department, Faculty of Medicine, Universitas Indonesia (FMUI), from July to December 2018. CYGB expression was inhibited by small interfering ribonucleic acid (siRNA) and CYGB. Analysis of mitochondrial function was observed through peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), a mitochondrial biogenesis marker and the activity of the succinate dehydrogenase (SDH) enzyme in mitochondria. Results: The CYGB gene and protein were downregulated after treatment with CYGB siRNA. Inhibition of CYGB expression with siRNA also tended to decrease the levels of PGC-1α messenger ribonucleic acid (mRNA) and protein, as well as SDH enzyme activity. Conclusion: Inhibition of CYGB expression with siRNA tended to decrease mitochondrial biogenesis and function. This may be useful for understanding the excessive proliferation of fibroblasts in keloids and for development of treatment for keloids. © Penerbit Universiti Sains Malaysia, 2021. |
CYGB siRNA; Keloid fibroblasts; PGC-1α; SDH enzyme activity |
cytoglobin; oxygen; peroxisome proliferator activated receptor gamma coactivator 1alpha; small interfering RNA; succinate dehydrogenase; Article; cell function; cell proliferation; colorimetry; controlled study; down regulation; enzyme activity; enzyme linked immunosorbent assay; fibroblast; gene expression; genetic transfection; human; human cell; in vitro study; keloid; mitochondrial biogenesis; mitochondrial genetics; mRNA expression level; protein expression; protein isolation; real time polymerase chain reaction; reverse transcription; RNA isolation |
Penerbit Universiti Sains Malaysia |
1394195X |
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Article |
Q3 |
394 |
12287 |
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808 |
Ambarsari C.G., Hidayati E.L., Tridjaja B., Mochtar C.A., Wulandari H.F., Harahap A.S., Grace A. |
57211850895;57200542624;6504507193;6506558321;57223434925;57218511857;57217085273; |
Silent Hypertensive Crisis in an Adolescent: First Case Report of Pediatric Pheochromocytoma from Indonesia |
2021 |
Global Pediatric Health |
8 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105884851&doi=10.1177%2f2333794X211015484&partnerID=40&md5=51b6ae929a36713aa5a073baeb034198 |
Faculty of Medicine, Universitas Indonesia - Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
Ambarsari, C.G., Faculty of Medicine, Universitas Indonesia - Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Hidayati, E.L., Faculty of Medicine, Universitas Indonesia - Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Tridjaja, B., Faculty of Medicine, Universitas Indonesia - Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Mochtar, C.A., Faculty of Medicine, Universitas Indonesia - Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Wulandari, H.F., Faculty of Medicine, Universitas Indonesia - Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Harahap, A.S., Faculty of Medicine, Universitas Indonesia - Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Grace, A., Faculty of Medicine, Universitas Indonesia - Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
Secondary hypertension in children, to the rare extent, can be caused by endocrine factors such as pheochromocytoma, an adrenal tumor that secretes catecholamine. Only a few cases have been reported in the past 3 decades. To the best of our knowledge, this is the first case report of pediatric pheochromocytoma from Indonesia. We reviewed a case of a 16-year-old Indonesian boy with history of silent hypertensive crisis who was referred from a remote area in an island to the pediatric nephrology clinic at Cipto Mangunkusumo Hospital, Jakarta, Indonesia. Despite medications, his symptoms persisted for 14 months. At the pediatric nephrology clinic, pheochromocytoma was suspected due to symptoms of catecholamine secretion presented, which was palpitation, diaphoresis, and weight loss. However, as the urine catecholamine test was unavailable in Indonesia, the urine sample was sent to a laboratory outside the country. The elevated level of urine metanephrine, focal pathological uptake in the right adrenal mass seen on 131I-MIBG, and histopathology examination confirmed the suspicion of pheochromocytoma. Following the tumor resection, he has been living with normal blood pressure without antihypertensive medications. This case highlights that pheochromocytoma should always be included in the differential diagnoses of any atypical presentation of hypertension. In limited resources setting, high clinical awareness of pheochromocytoma is required to facilitate prompt referral. Suspicion of pheochromocytoma should be followed by measurement of urine metanephrine levels. Early diagnosis of pheochromocytoma would fasten the optimal cure, alleviate the symptoms of catecholamine release, and reverse hypertension. © The Author(s) 2021. |
adrenal glands; blood pressure; epinephrine; metanephrine; MIBG; norepinephrine |
bisoprolol; captopril; catecholamine; creatinine; furosemide; liothyronine; nebivolol; nifedipine; telmisartan; terazosin; vitamin D; adolescent; adrenalectomy; Article; case report; clinical article; diaphoresis; Doppler flowmetry; free thyroxine index; heart palpitation; human; hypertension; hypertensive crisis; Indonesian; kidney artery stenosis; male; nuclear magnetic resonance imaging; pheochromocytoma; scintigraphy; tachycardia |
SAGE Publications Inc. |
2333794X |
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Article |
Q2 |
354 |
13264 |
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