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606 |
Hanifah N., Wiweko B., Bowolaksono A. |
57200075370;43061741400;57205093224; |
FSHR and LHR mRNA expression in granulosa cells of poor responder |
2021 |
Journal of Physics: Conference Series |
1725 |
1 |
012057 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85100793552&doi=10.1088%2f1742-6596%2f1725%2f1%2f012057&partnerID=40&md5=498a20aef40d78ffcc6b9fa74da8dec6 |
Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok, 16424, Indonesia; Department of Obstetric and Gynecology, Faculty of Medicine, Universitas Indonesia, Depok, 16424, Indonesia |
Hanifah, N., Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok, 16424, Indonesia; Wiweko, B., Department of Obstetric and Gynecology, Faculty of Medicine, Universitas Indonesia, Depok, 16424, Indonesia; Bowolaksono, A., Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok, 16424, Indonesia |
In Vitro Fertilization (IVF) is one of the most commonly used procedures to help pregnancies in couples who have infertility problems. One of the problems of infertility is poor ovarian response and the woman who experiences it is known as poor responder. Poor responders do not have an adequate ovarian response to gonadotropin in ovarian stimulation. The success of fertilization in poor responders tends to be low due to low quantity and is generally followed by low oocyte quality. Gonadotropins consisting of FSH and LH, play a role in follicle development and ovulation. The follicle response in capturing gonadotropins depends on the exact bond between the hormone and its receptor (FSHR and LHR) in the granulosa cells surrounding the oocyte. The purpose of this research is to know the expression level of fshr and lhr mRNA in granulosa cells of poor responders through real-time PCR method which then tested statistically using t-test. Fourteen samples of each poor responders and normal women were used in this research. The results showed insignificant differences between expression level of fshr and lhr mRNA in granulosa cells of poor responders and normal women (p > 0.05). © 2021 Journal of Physics: Conference Series. |
FSHR; LHR; Poor ovarian response; Real-time PCR |
Cytology; Polymerase chain reaction; Expression levels; Follicle development; Granulosa cells; In-vitro; mRNA expression; Real-time PCR method; T-tests; Cells |
IOP Publishing Ltd |
17426588 |
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Conference Paper |
Q4 |
210 |
18731 |
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607 |
Lestari D.A., Abdullah S., Rachman A. |
56119337300;57204563168;57217184320; |
Identification of factors associated with hypothyroidism due to radiotherapy in patients with nasopharyngeal cancer (Case study of nasopharyngeal cancer in one of the hospitals in Jakarta) |
2021 |
Journal of Physics: Conference Series |
1725 |
1 |
012027 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85100793360&doi=10.1088%2f1742-6596%2f1725%2f1%2f012027&partnerID=40&md5=eb87ed0fd4bc75637854a08478652d08 |
Department of Mathematics, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok, 16424, Indonesia; Division of Hematology and Medical Oncology, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia |
Lestari, D.A., Department of Mathematics, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok, 16424, Indonesia; Abdullah, S., Department of Mathematics, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok, 16424, Indonesia; Rachman, A., Division of Hematology and Medical Oncology, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia |
Nasopharyngeal cancer is an abnormal cell growth that develops around the nasopharynx. Treatment of nasopharyngeal cancer patients includes chemotherapy or radiotherapy. Both treatments have side effects in patients. In this study, we will focus on hypothyroidism as a side effect of radiotherapy in the treatment of patients with nasopharyngeal cancer. Hypothyroidism is a condition when the thyroid gland is unable to produce enough thyroid hormone. The main goal of this study is to identify the factors associated with hypothyroidism. To achieve this goal, classification tree and logistic regression methods will be used. Classification tree is used to obtain important variables in the classification of subject classes. Then, logistic regression is used to quantify the risk of variables that appear in the classification tree, hypothyroidism risk factors, and hypothyroidism marker factors. Based on the analysis, it was found that the factors associated in this study were variable symptom, physical sign, smoking habits, gender, age, BMI (Body Mass Index), TSH (Thyroid Stimulating Hormone) and fT4 (free thyroxine) hormones, and also all items on Zulewski score, except items delayed ankle reflex and slow movements. These factors associated tended to increase the risk of hypothyroidism, except for the fT4 hormone and BMI. © 2021 Journal of Physics: Conference Series. |
Classification tree; Hypothyroidism; Logistic regression; Marker factors; Risk factors |
Cell proliferation; Chemotherapy; Hormones; Logistic regression; Radiotherapy; Body mass index; Classification trees; Logistic regression method; Nasopharyngeal cancer; Risk factors; Thyroid glands; Thyroid hormones; Thyroid stimulating hormones; Diseases |
IOP Publishing Ltd |
17426588 |
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Conference Paper |
Q4 |
210 |
18731 |
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608 |
Lukman P.A., Abdullah S., Rachman A. |
57221968648;57204563168;57217184320; |
Bayesian logistic regression and its application for hypothyroid prediction in post-radiation nasopharyngeal cancer patients |
2021 |
Journal of Physics: Conference Series |
1725 |
1 |
012010 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85100754485&doi=10.1088%2f1742-6596%2f1725%2f1%2f012010&partnerID=40&md5=902f22d97f61ad13843e9bbe6a66f42e |
Department of Mathematics, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok, 16424, Indonesia; Department of Medical Education, Faculty of Medicine, Universitas Indonesia, Depok, 16424, Indonesia |
Lukman, P.A., Department of Mathematics, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok, 16424, Indonesia; Abdullah, S., Department of Mathematics, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok, 16424, Indonesia; Rachman, A., Department of Medical Education, Faculty of Medicine, Universitas Indonesia, Depok, 16424, Indonesia |
Logistic regression models are commonly used to model response variables in the form of categorical variables with several predictor variables. The contribution of the predictor variable to the response variable is expressed through a regression coefficient (β). Therefore, it is necessary to estimate β. This study discusses the estimation of β using the Bayesian method. Bayesian approach utilizes a combination of information from sample data and prior information about the characteristics of the parameters of interest, resulting in the updated information, namely the posterior. Bayesian method thus can overcome the problem if the quality of the sample data does not support observation. Bayesian logistic regression method will be used in analyzing post-radiation nasopharyngeal cancer (NPC) patient data, using measurement on Zulewski's score components. Markov Chain Monte Carlo with Gibbs Sampling were used to obtain the sample from posterior distribution. Convergent estimates were obtained, and the result showed that Zulewski's component scores only were not enough to explain the hypothyroidism in NPC. Additional information is required in order to explain the incidence of hypothyroidism in NPC. © 2021 Journal of Physics: Conference Series. |
Bayesian logistic regression; Gibbs sampling; Logistic regression; Markov chain monte carlo; Nasopharyngeal cancer |
Bayesian networks; Diseases; Hospital data processing; Markov chains; Radiotherapy; Categorical variables; Logistic regression method; Logistic regression models; Markov Chain Monte-Carlo; Nasopharyngeal cancer; Posterior distributions; Regression coefficient; Updated informations; Logistic regression |
IOP Publishing Ltd |
17426588 |
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Conference Paper |
Q4 |
210 |
18731 |
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609 |
Amelia V., Siswantining T., Kamelia T. |
57221954910;57193446800;35603752000; |
Prediction model of exacerbations in patients with Chronic Obstructive Pulmonary Disease (COPD) at RSCM |
2021 |
Journal of Physics: Conference Series |
1725 |
1 |
012011 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85100735594&doi=10.1088%2f1742-6596%2f1725%2f1%2f012011&partnerID=40&md5=3f0c08db1ff574eac108d1a10d8c68a9 |
Department of Mathematics, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok, 16424, Indonesia; Division of Pulmonology, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia |
Amelia, V., Department of Mathematics, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok, 16424, Indonesia; Siswantining, T., Department of Mathematics, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok, 16424, Indonesia; Kamelia, T., Division of Pulmonology, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia |
Chronic Obstructive Pulmonary Disease (COPD) is a worldwide health problem. COPD has a tendency for exacerbations. Exacerbations are worsening of acute respiratory symptoms resulting in additional therapy. Exacerbations in COPD increase the risk of death. The objective of this study is to determine the prediction model of exacerbations in patients with COPD based on factors affecting exacerbations in patients with COPD at RSCM (Rumah Sakit Cipto Mangunkusumo). The data used in this study is secondary data from the medical records of patients with COPD in RSCM. The sample was chosen using purposive sampling technique. The samples in this study are 107 patients with COPD. The method used is binary logistic regression analysis. The results of this study indicate that the factors that significantly influence the exacerbations of COPD are breathlessness, history of ICS use, and history of antibiotics use. Appropriate logistic regression model has been obtained. The result indicates that patients with COPD who have breathlessness, have history of ICS use, and have history of antibiotics use are more at risk of exacerbations than those who don't. Accuracy test has been conducted with classification table at cut point 0.5. The prediction model has an accuracy rate of 74.77 %. © 2021 Journal of Physics: Conference Series. |
COPD; Exacerbations; Logistic regression |
Antibiotics; Forecasting; Logistic regression; Predictive analytics; Binary logistic regression; Chronic obstructive pulmonary disease; Logistic Regression modeling; Medical record; Prediction model; Respiratory symptoms; Sampling technique; Secondary datum; Pulmonary diseases |
IOP Publishing Ltd |
17426588 |
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Conference Paper |
Q4 |
210 |
18731 |
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610 |
Lisnawati L., Billianti Y.D., Manatar A.F. |
57215311695;57398065300;57398065400; |
Association between foxp3 tumor infiltrating lymphocyte expression and response after chemoradiation in nasopharyngeal carcinoma |
2021 |
Open Access Macedonian Journal of Medical Sciences |
9 |
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1285 |
1291 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122331446&doi=10.3889%2foamjms.2021.7639&partnerID=40&md5=966b01cb054ca6692343d8dda3943b56 |
Department of Anatomical Pathology, Faculty of Medicine, University of Indonesia, Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia |
Lisnawati, L., Department of Anatomical Pathology, Faculty of Medicine, University of Indonesia, Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia; Billianti, Y.D., Department of Anatomical Pathology, Faculty of Medicine, University of Indonesia, Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia; Manatar, A.F., Department of Anatomical Pathology, Faculty of Medicine, University of Indonesia, Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia |
BACKGROUND: Nasopharyngeal carcinoma (NPC) is a carcinoma originating from the surface epithelium of the nasopharynx with the highest incidence in China and South East Asia. Currently, many researchers are developing tumor microenvironment which can be assessed by tumor-infiltrating lymphochyte, and its association with treatment response in several tumors, including NPC. Foxp3, known as a regulatory T cell (Treg) marker, plays a role in the immunoregulatory environment of tumor cells and can be used as a prognostic factor. The relationship between Foxp3 expression and treatment response is considered as one of the factors affecting the prognosis of NPC. AIM: This study aims to determine the relationship between Foxp3 expression and treatment response in NPC. MATERIALS AND METHODS: A cross-sectional study was done to analyze the association between Foxp3 and treatment response in NPC. This study included 60 samples who were diagnosed with non-keratinizing NPC at the Department of Anatomical Pathology, Faculty of Medicine Universitas Indonesia/Cipto Mangunkusumo Hospital from January 2018 until December 2020. Immunohistochemistry was done to evaluate the expression of Foxp3. Foxp3 expression was evaluated in the intratumoral and peritumoral areas. RESULTS: Among 60 patients, the number of males were more than females (66.7%, 33.3%, respectively) with a ratio of 2:1. There was statistically significant difference between intratumoral and total Foxp3 expression and treatment response (p < 0.05, p = 0.001, respectively); however, no significant differences found between peritumoral Foxp3 expression and treatment response (p = 0.114). CONCLUSION: Foxp3 expression had a statistically significant relationship with response therapy after chemoradiation. © 2021 Lisnawati Lisnawati, Yayi Dwina Billianti, Amelia Fossetta Manatar. |
Chemoradiation therapy; Foxp3; Nasopharyngeal carcinoma |
transcription factor FOXP3; adolescent; adult; Article; cancer infiltration; cancer prognosis; chemoradiotherapy; chemotherapy; child; complete response; computer assisted tomography; cross-sectional study; female; gene expression; histopathology; human; human tissue; immunohistochemistry; lymph vessel metastasis; lymphocytic infiltration; major clinical study; male; nasopharynx carcinoma; nuclear magnetic resonance imaging; partial response; pathologist; progressive response; protein expression; radiodiagnosis; regulatory T lymphocyte; rehydration; stable disease; treatment response; tumor associated leukocyte; tumor microenvironment; tumor volume |
Scientific Foundation SPIROSKI |
18579655 |
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Article |
Q3 |
288 |
15252 |
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611 |
Indarti J., Surya R., Aditya R., Ikhsan M., Alda K., Antoniman M.A. |
39161587400;56986345100;57384331700;36518708700;57218391373;57385259100; |
Successfully pregnancy outcome after lletz in women with cervical intraepithelial neoplasia: A case series |
2021 |
Open Access Macedonian Journal of Medical Sciences |
9 |
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308 |
312 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121712103&doi=10.3889%2foamjms.2021.7770&partnerID=40&md5=c8d73616847c9c707c197e7f03bfed5e |
Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Indarti, J., Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Surya, R., Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Aditya, R., Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Ikhsan, M., Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Alda, K., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Antoniman, M.A., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
BACKGROUND: Human papillomavirus (HPV) has an important role in cervical cancer development and the incidence of cervical intraepithelial neoplasia (CIN) was 1.3–2.7/1000 pregnancies. The HPV and its treatments such as loop electrosurgical excisional procedure (LEEP) or large loop electrosurgical excisional procedure (LLETZ) have an association with poor obstetric outcomes. CASE REPORT: Here, we present four case studies of successful live birth after treatment of CIN. We reported that four patients had been performed LLETZ, with abnormal colposcopy results and liquidbased cytology results were one ASCUS, one ASCH, and two HSIL. The histopathology results were one CIN 1, one CIN 2, and two CIN 3. There was a higher rate of pregnancy for treated women than untreated women. The higher the CIN grades, the more prevalence of cesarean section rate. CONCLUSION: The HPV testing or cotesting at 3-year intervals is recommended after treatment due to the sensitivity of HPV testing. Although pregnancy could delay the progression of precancerous lesions, it is recommended to follow the individualized algorithm in the ASCCP guideline to reduce the risk of cervical cancer progression. © 2021 Junita Indarti, Raymond Surya, Reyhan Aditya, Muhammad Ikhsan, Kristian Alda, Mohammad Agassi Antoniman. |
Infant outcome; Precancerous lesion; Pregnancy profile |
adult; algorithm; Article; birth weight; cancer growth; case report; case study; cesarean section; clinical article; colposcopy; cytology; family planning; female; histopathology; human; live birth; loop electrosurgical excision; practice guideline; preeclampsia; pregnancy outcome; uterine cervix carcinoma in situ; Wart virus |
Scientific Foundation SPIROSKI |
18579655 |
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Article |
Q3 |
288 |
15252 |
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612 |
Astuti S.W., Liem I.K., Ramli Y. |
57385798200;55802927800;57197869497; |
The effect of intravenously and intra-arterially delivered human umbilical cord blood mononuclear cell on cortical neurogenesis of post-ischemic stroke rat brain |
2021 |
Open Access Macedonian Journal of Medical Sciences |
9 |
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1245 |
1251 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121681681&doi=10.3889%2foamjms.2021.6555&partnerID=40&md5=33a2aafda87f7fa7e1e4defa3f943842 |
Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Anatomy, Faculty of Medicine, Gunadarma University, Depok, Indonesia; Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Stem Cell Medical Technology Integrated Service Unit, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Stem Cell and Tissue Engineering Cluster, Indonesia Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Neurology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National Hospital, Jakarta, Indonesia |
Astuti, S.W., Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Department of Anatomy, Faculty of Medicine, Gunadarma University, Depok, Indonesia; Liem, I.K., Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Stem Cell Medical Technology Integrated Service Unit, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia, Stem Cell and Tissue Engineering Cluster, Indonesia Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Ramli, Y., Stem Cell Medical Technology Integrated Service Unit, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia, Department of Neurology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National Hospital, Jakarta, Indonesia |
BACKGROUND: Stroke is the second most cause of death in the world. There are several treatments but they often end up with disabilities. Recently, cell therapy has become a new hope as an alternative treatment as it could improve the patients neurological deficits and daily living activities. Cord blood mononuclear cells (CB-MNCs) are one of the cell therapies for post-ischemic neurogenesis by intravenous or intra-arterial administration; however, it is not clear which one is better. AIM: This study aims to compare the effects of intra-arterial and intravenous administration of human CB-MNC on cortical neurogenesis of rat brain after ischemic stroke. METHODS: Twenty-four rats were divided into four groups, that is, control, middle cerebral artery obstruction (MCAO) without treatment, MCAO with intra-arterial CB-MNC injection (MCAO-IA), and MCAO with intravenous CB-MNC injection (MCAO-IV). Two weeks after injection, all rats were sacrificed, the brain was harvested, histologically process and stained with hematoxylin eosin (HE) to determine cellular and tissue morphology changes, and immunohistochemical staining, anti-NeuN antibody to determine the number of cortical neurons. The HE showed that MCAO rat brain had gliosis and shrunken cells. RESULTS: The results showed that MCAO-IA and MCAO-IV had fewer areas of gliosis and shrunken cells when compared to the MCAO group. The number of neurons also showed an increase. However, there was no difference between the MCAO-IA and MCAO-IV groups. It was concluded both of them could improve neurogenesis. CONCLUSION: CB-MNC administration can be an alternative for stroke ischemic therapy because it is proven to increase neurogenesis and reduce gliosis areas. However, there was no difference in neurogenesis in the brain tissue of mice injected with CB-MNC intravenously or intra-arterially. © 2021, Scientific Foundation SPIROSKI. All rights reserved. |
Cord blood mononuclear cells; Ischemic stroke; NeuN; Neurogenesis |
anti NeuN antibody; ketamine; neuron specific nuclear protein; unclassified drug; xylazine; animal experiment; animal model; animal tissue; Article; brain cell; cell therapy; controlled study; gliosis; immunohistochemistry; intraarterial drug administration; intravenous drug administration; ischemic stroke; male; middle cerebral artery occlusion; mononuclear cell; nervous system development; nonhuman; rat; umbilical cord blood |
Scientific Foundation SPIROSKI |
18579655 |
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Article |
Q3 |
288 |
15252 |
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613 |
Margiana R., Alawiyah K., Ima K., Fitriana R., Widodo A.R., Wibowo T.D. |
56685900600;57212465522;57210444835;57377515100;57377515200;57377674000; |
Improvement of walking analysis using the sciatic function index for sciatic nerve function in injured rat model treated with low-intensity aerobics |
2021 |
Open Access Macedonian Journal of Medical Sciences |
9 |
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1162 |
1168 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121438837&doi=10.3889%2foamjms.2021.7289&partnerID=40&md5=16b490d881d3f49901d8a12bcb6ef892 |
Departement of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Salemba, Indonesia; Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Margiana, R., Departement of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Salemba, Indonesia; Alawiyah, K., Departement of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Salemba, Indonesia; Ima, K., Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Fitriana, R., Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Widodo, A.R., Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Wibowo, T.D., Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
BACKGROUND: Sciatica is a disease of the peripheral nerves. Sciatica shows that there is damage to the peripheral nerves in the sciatic nerves. Factors that can affect this disease include gender, posture, parity, age, genetic factors, and occupation. Some of the pathophysiological conditions of sciatica include the pathology of the intervertebral disc, dorsal root, and sciatic nerve itself. The results of standard therapy with surgery have not been effective and very expensive. Therefore, research on therapy in sciatica cases still needs to be done and evaluated. Physical exercise treatment (aerobic) is necessary for this therapy in sciatica cases due to promote the function of peripheral nerves. AIM: This study aimed to determine the effect of aerobic exercise treatment on peripheral nerve injury and its relationship to walking function during injury-induced peripheral nerve regeneration. METHODS: This study was an experimental study with a post-test. The study sample comprised male Sprague-Dawley rats with an age of about 2–3 months divided into three groups. The control group was conducted by surgery without clamping/injuring the peripheral nerves. The treatment for the second group (P1) was clamping/injury of peripheral nerve and given the treatment of physical exercise with aerobics. The treatment for the third group (P2) was clamping/injury of peripheral nerve and not given the treatment of physical exercise with aerobics. The intensity of giving physical exercise treatment with aerobic that is carried out is for 42 days. Nerve functional evaluation was carried out using the sciatic function index (SFI) method. Histological staining for sciatic was used for hematoxylin-eosin staining and immunohistochemistry with growth-associated protein 43 (GAP43) [Bioss, bs-0154R] and S100 antibody [ab52642]. This research was approved by the Animal Ethics Committee of the University of Indonesia protocol (No.19-07-0852). RESULTS: There was a significant change between the 7th and 14th days (p < 0.001; paired t-test) in the P1 treatment. Improvement in nerve function was found on the 14th day after being given aerobic treatment. This is showed by the data average change in SFI scores on days 7 and 4 was from −144 to 34. These data are also supported by footprint changes for injured hindfoot data. CONCLUSION: Low-intensity aerobics treatment improves the walking function and nerve function in sciatic nerve injury on day 14. This is due to the effect of physical exercise on the injured sciatic nerve. © 2021 Ria Margiana, Kamila Alawiyah, Khoirul Ima, Rizni Fitriana, Arif Rahmat Widodo, Theresa Devi Wibowo. |
Aerobics; Physical exercise; Sciatic fuction index; Sciatic nerve injury |
eosin; hematoxylin; neuromodulin; aerobic exercise; animal experiment; animal model; Article; controlled study; demyelination; histology; histopathology; immunohistochemistry; ischiadicus function index; kinesiotherapy; low intensity aerobics; male; muscle weakness; nerve function; nerve injury; nerve regeneration; nonhuman; parameters; peripheral nerve injury; physical activity; rat; rat model; sciatic function index; sciatic nerve; Sprague Dawley rat; walking function |
Scientific Foundation SPIROSKI |
18579655 |
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Article |
Q3 |
288 |
15252 |
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614 |
Rhatomy S., Setyawan R., Romulo M.A. |
57204509883;57206249736;57221505201; |
Enhancement of chondrogenesis in hypoxic precondition culture: A systematic review |
2021 |
Open Access Macedonian Journal of Medical Sciences |
9 |
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492 |
504 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118498577&doi=10.3889%2foamjms.2021.5850&partnerID=40&md5=a767ee0e694f65e29d11b57b920b1f48 |
Department of Orthopaedics and Traumatology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia; Department of Orthopaedics and Traumatology, Sport and Adult Reconstructive Division, Dr. Soeradji, Tirtonegoro General Hospital, Klaten, Indonesia; Department of Orthopaedics and Traumatology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Soeradji Tirtonegoro Sport Center and Research Unit, Dr. Soeradji Tirtonegoro General Hospital, Klaten, Indonesia |
Rhatomy, S., Department of Orthopaedics and Traumatology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia, Department of Orthopaedics and Traumatology, Sport and Adult Reconstructive Division, Dr. Soeradji, Tirtonegoro General Hospital, Klaten, Indonesia, Department of Orthopaedics and Traumatology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Setyawan, R., Department of Orthopaedics and Traumatology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Romulo, M.A., Soeradji Tirtonegoro Sport Center and Research Unit, Dr. Soeradji Tirtonegoro General Hospital, Klaten, Indonesia |
BACKGROUND: Cartilage tear has begun to be treated with stem cells. However, stem cell oxygen level culture has not been evaluated for the best environment to enhance chondrogenesis. AIM: The purpose of this review is to focus on the hypoxic oxygen level of stem cells culture as a treatment for cartilage tear. METHODS: A literature search was systemically conducted on PubMed (MEDLINE), OVID, EMBASE, the Cochrane Library, Scopus, Web of Science, Science Direct, Wiley Online Library, Google Scholar, and bibliography of selected articles with the terms (“culture”) AND (“stem cell” OR “mesenchymal stem cell” OR “MSC”) AND (“hypoxic” OR “hypoxia”) AND (“cartilage” OR “chondro*”) as the main keywords. A total of 438 articles were reviewed. Thirty-six articles were considered relevant for this systematic review. RESULTS: The result of this review supports stimulation effects of hypoxic oxygen level stem cell culture in chondrogenesis process. Most studies used 5% oxygen concentration for culture, both of in vivo and in vitro studies. Due to the heterogeneity nature of the included studies, meta-analysis was unable to be conducted. CONCLUSION: Hypoxia state seems to play an important role in chondrocytes proliferation, differentiation, and matrix production. © 2021 Sholahuddin Rhatomy,. |
Cartilage; Hypoxic; Oxygen level; Stem cell |
amphotericin; collagen type 1; collagen type 10; collagen type 2; elastin; glycosaminoglycan; hydroxyproline; oxygen; penicillin G; probiotic agent; streptomycin; stromal cell derived factor 1; Thy 1 membrane glycoprotein; adipose derived stem cell; biomechanics; cell culture technique; cell differentiation; cell isolation; cell proliferation; chondrogenesis; Cochrane Library; Embase; flow cytometry; gene expression; histology; human; human cell; hypoxia; hypoxic precondition culture; iliac crest; in vitro fertilization; in vitro study; in vivo study; mesenchymal stem cell; mesenchymal stem cell transplantation; natural killer cell; osteoblast; oxygen concentration; oxygen tension; phylogenetic tree; protein expression; quality control; Review; ScienceDirect; Scopus; search engine; stem ce |
Scientific Foundation SPIROSKI |
18579655 |
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Review |
Q3 |
288 |
15252 |
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615 |
Rahadiani N., Retnowulan I.A., Stephanie M., Handjari D.R., Krisnuhoni E. |
16426455700;57313230400;57192369336;56160657900;35310982200; |
β-Catenin Expression and Its Association with Prognostic Factors in Hepatocellular Carcinoma: A Study on Alpha-fetoprotein, Histologic Grade, and Microvascular Invasion |
2021 |
Open Access Macedonian Journal of Medical Sciences |
9 |
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887 |
893 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117945095&doi=10.3889%2foamjms.2021.6123&partnerID=40&md5=76f7c7436e4af55617330cb33cb4138f |
Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia |
Rahadiani, N., Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Retnowulan, I.A., Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Stephanie, M., Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Handjari, D.R., Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Krisnuhoni, E., Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia |
BACKGROUND: Hepatocellular carcinoma (HCC) is the most common primary liver cancer. In addition to its high incidence, the disease burden is high due to its poor prognosis and high recurrence rate. Some of the currently known clinicopathologic prognostic factors include alpha-fetoprotein (AFP) level, histologic grade, and microvascular invasion. At the molecular level, β-catenin is one of the most common driver mutations found in HCC. The Wnt/β-catenin pathway regulates cellular processes related to initiation, growth, survival, migration, differentiation, and apoptosis. Although the underlying pathogenesis of hepatocarcinogenesis is known, clinical application warrants a greater understanding of the molecular characteristics and tumor phenotype, especially for determining the prognosis. AIM: This study aims to analyze the expression of β-catenin and its association with AFP, histologic grade, and microvascular invasion. METHODS: Thirty-five samples of surgically resected HCCs at Cipto Mangunkusumo National Referral Hospital were examined. Diagnoses were made based on histopathological and immunohistochemical findings followed by β-catenin staining. β-catenin expression was analyzed to determine difference between variables. RESULTS: Here, we show that β-catenin expression is significantly associated with low serum AFP and well to moderate differentiation implications. Strong nuclear β-catenin expression implies better prognosis in HCC. CONCLUSION: β-catenin expression is significantly associated with low serumAFP and well-to-moderate differentiation, but not with microvascular invasion. Strong nuclear β-catenin expression implies better prognosis in HCC. © 2021 Nur Rahadiani, Ignasia Andhini Retnowulan, Marini Stephanie, Diah Rini Handjari, Ening Krisnuhoni. |
Alpha-fetoprotein; Hepatocellular carcinoma; Microvascular invasion; Tumor grade; β-catenin |
alpha fetoprotein; beta catenin; adult; aged; alpha fetoprotein blood level; Article; cancer grading; cancer prognosis; cell invasion; clinical article; controlled study; female; hepatitis B; hepatitis C; histology; histopathology; human; human tissue; immunohistochemistry; liver cell carcinoma; liver cirrhosis; lymph vessel; male; microvasculature; prevalence; protein expression; risk factor; tumor growth; tumor volume |
Scientific Foundation SPIROSKI |
18579655 |
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Article |
Q3 |
288 |
15252 |
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