389 |
Bangun K., Sukasah C.L., Dilogo I.H., Indrani D.J., Siregar N.C., Pandelaki J., Iskandriati D., Kekalih A., Halim J. |
36902624600;57218391534;56161962800;6602774472;6508087790;35759266900;6507742011;55633562200;57219398869; |
Bone Growth Capacity of Human Umbilical Cord Mesenchymal Stem Cells and BMP-2 Seeded Into Hydroxyapatite/Chitosan/Gelatin Scaffold in Alveolar Cleft Defects: An Experimental Study in Goat |
2021 |
Cleft Palate-Craniofacial Journal |
58 |
6 |
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707 |
717 |
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1 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092575595&doi=10.1177%2f1055665620962360&partnerID=40&md5=a3280d360d1de28c8353dffc23d716b3 |
Department of Plastic and Reconstructive Surgery, Cleft and Craniofacial Center, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Unit Pelayanan Terpadu Teknologi Kedokteran Sel Punca (Stem Cell Research Center), Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Department of Dental Material Science and Technology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia; Anatomical Pathology Department, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Radiology Department of Cipto, Mangunkusumo General Hospital, Jakarta, Indonesia; Primate Research Center of Bogor Agricultural Institute, Bogor, Indonesia; Community Medicine Department, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Cipto Mangunkusumo General Hospital, Jakarta, Indonesia |
Bangun, K., Department of Plastic and Reconstructive Surgery, Cleft and Craniofacial Center, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Sukasah, C.L., Department of Plastic and Reconstructive Surgery, Cleft and Craniofacial Center, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Dilogo, I.H., Unit Pelayanan Terpadu Teknologi Kedokteran Sel Punca (Stem Cell Research Center), Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Indrani, D.J., Department of Dental Material Science and Technology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia; Siregar, N.C., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Anatomical Pathology Department, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Pandelaki, J., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Radiology Department of Cipto, Mangunkusumo General Hospital, Jakarta, Indonesia; Iskandriati, D., Primate Research Center of Bogor Agricultural Institute, Bogor, Indonesia; Kekalih, A., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Community Medicine Department, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Halim, J., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia |
Objective: To evaluate bone regeneration in alveolar defects treated with human umbilical cord–derived mesenchymal stem cells (hUCMSCs), hydroxyapatite/chitosan/gelatin (HA/CS/Gel) scaffold, and bone morphogenic protein-2 (BMP-2) in Capra hircus models. Design: Randomized posttest-only control group design. Setting: Animal Hospital at Bogor Agricultural Institute. Participants: Healthy and equally treated 24 female Capra hircus/goats. Intervention: Animals were randomly assigned to 3 experimental group design (iliac crest alveolar bone graft/ICABG [control], HA/Cs/Gel+BMP-2 [Novosys], and HA/Cs/Gel+BMP-2+UCMSCs). Graft materials were implanted in surgically made alveolar defects. Main Outcome Measures: Postoperative functional score and operating time were assessed. New bone growth, bone density, inflammatory cells recruitment, and neoangiogenesis were evaluated based on radiological and histological approach at 2 time points, week 4 and 12. Statistical analysis was done between treatment groups. Results: Operating time was 34% faster and functional score 94.5% more superior in HA/Cs/Gel+BMP-2+hUCMSC group. Bone growth capacity in HA/Cs/Gel+BMP-2+UCMSCs mimicked ICABG, but ICABG showed possibility of bone loss between week 4 and 12. The HA/Cs/Gel+BMP-2+UCMSCs showed early bone repopulation and unseen inflammatory cells and angiogenesis on week 12. Discussion and Conclusion: The HA/Cs/Gel+BMP-2+hUCMSCs were superior in enhancing new bone growth without donor site morbidity compared to ICABG. The presence of hUCMSCs in tissue-engineered alveolar bone graft (ABG), supported with paracrine activity of the resident stem cells, initiated earlier new bone repopulation, and completed faster bone regeneration. The HA/Cs/Gel scaffold seeded with UCMSCs+BMP-2 is a safe substitute of ICABG to close alveolar bone defects suitable for patients with cleft lip, alveolus, and palate. © American Cleft Palate-Craniofacial Association. All rights reserved 2020. |
alveolar bone grafts; bone regeneration; hydroxyapatite/chitosan/gelatin scaffold; iliac crest bone graft; mesenchymal stem cells; tissue engineering |
bone morphogenetic protein 2; chitosan; gelatin; hydroxyapatite; gelatin; hydroxyapatite; adult; alveolar bone; alveolar bone grafting; angiogenesis; animal experiment; animal model; animal tissue; Article; bone density; bone graft; bone growth; bone regeneration; controlled study; domestic goat; donor site; female; histology; human; human cell; iliac crest; inflammatory cell; jaw malformation; morbidity; nonhuman; operation duration; osteolysis; outcome assessment; paracrine signaling; umbilical cord mesenchymal stem cell; animal; bone development; goat; mesenchymal stem cell; tissue scaffold; umbilical cord; Animals; Bone Development; Bone Regeneration; Chitosan; Durapatite; Female; Gelatin; Goats; Humans; Mesenchymal Stem Cells; Tissue Scaffolds; Umbilical Cord |
SAGE Publications Ltd |
10556656 |
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34047209 |
Article |
Q2 |
641 |
7859 |
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643 |
Bangun K., Halim J., Tania V. |
36902624600;57219398869;57394760300; |
Repair of Protruding Bilateral Cleft Lip and Palate With Staged Premaxilla Setback Osteotomy, Cheiloplasty, and Palatoplasty in Trisomy 17p Patient: A Review of Syndromic Clinical Characteristic |
2021 |
Cleft Palate-Craniofacial Journal |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122134129&doi=10.1177%2f10556656211069820&partnerID=40&md5=065c5cf3acef75c03e60e1e90acf33a2 |
Cleft and Craniofacial Center, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Division of Plastic Reconstructive and Aesthetic Surgery, Department of Surgery, Faculty of Medicine Universitas Indonesia, Indonesia |
Bangun, K., Cleft and Craniofacial Center, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Division of Plastic Reconstructive and Aesthetic Surgery, Department of Surgery, Faculty of Medicine Universitas Indonesia, Indonesia; Halim, J., Cleft and Craniofacial Center, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Division of Plastic Reconstructive and Aesthetic Surgery, Department of Surgery, Faculty of Medicine Universitas Indonesia, Indonesia; Tania, V., Cleft and Craniofacial Center, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Division of Plastic Reconstructive and Aesthetic Surgery, Department of Surgery, Faculty of Medicine Universitas Indonesia, Indonesia |
Objective : Chromosome 17 duplication is correlated with an increased risk of developmental delay, birth defects, and intellectual disability. Here, we reported a female patient with trisomy 17 on the whole short arm with bilateral complete cleft lip and palate (BCLP). This study will review the surgical strategies to reconstruct the protruding premaxillary segment, cleft lip, and palate in trisomy 17p patient. Case Presentation : The patient had heterozygous pathogenic duplication of chromosomal region chr17:526-18777088 on almost the entire short arm of chromosome 17. Beside the commonly found features of trisomy 17p, the patient also presented with BCLP with a prominent premaxillary portion. Premaxillary setback surgery was first performed concomitantly with cheiloplasty. The ostectomy was performed posterior to the vomero-premaxillary suture (VPS). The premaxilla was firmly adhered to the lateral segment and the viability of philtral flap was not compromised. Two-flap palatoplasty with modified intravelar veloplasty (IVV) was performed 4 months after. Conclusion : Successful positioning of the premaxilla segment, satisfactory lip aesthetics, and vital palatal flap was obtained from premaxillary setback, primary cheiloplasty, and subsequent palatoplasty in our trisomy 17p patient presenting with BLCP. Postoperative premaxillary stability and patency of the philtral and palatal flap were achieved. Longer follow-up is needed to evaluate the long-term effects of our surgical techniques on inhibition of midfacial growth. However, the benefits that the patient received from the surgery in improving feeding capacity and facial appearance early in life outweigh the cost of possible maxillary retrusion. © 2021, American Cleft Palate-Craniofacial Association. |
bilateral cleft lip and palate; premaxillary osteotomy; trisomy 17 |
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SAGE Publications Ltd |
10556656 |
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Review |
Q2 |
641 |
7859 |
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