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No records
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131 |
Maulahela H., Fauzi A. |
57189612709;36518523000; |
Peripancreatic Tuberculosis Lymphadenopathy: The Role of Endoscopic Ultrasound for Diagnosis |
2021 |
Acta medica Indonesiana |
53 |
4 |
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457 |
459 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123566183&partnerID=40&md5=db2cd4e2d6a90740d0bc3e39263c3c1d |
Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo HospitalJakarta, Indonesia |
Maulahela, H., Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo HospitalJakarta, Indonesia; Fauzi, A. |
Pancreatic and peripancreatic tuberculosis is a rare abdominal tuberculosis. Diagnosis for pancreatic tuberculosis can be challenging. Conventional imaging tools may show mass or malignancy in the pancreas. Endoscopic ultrasound (EUS) is an excellent tools for evaluating pancreas and peri pancreas region. It also allows us to obtain tissue sample for cytology and histopathology. Here we present a case of peripancreatic tuberculosis lymphadenopathy that mimic pancreatic mass. His symptoms were also nonspecific (weight loss, epigastric pain, and irregular fever). From EUS evaluation we found that there was no mass but multiple lymphadenopathy around the pancreas and then performed FNA. The result of the cytology was granuloma inflammation and caseous necrosis which is compatible with tuberculosis infection. From this case illustration we conclude that EUS is an important diagnostic tool for pancreatic lesion to avoid unnecessary surgery. |
endoscopic ultrasound; lymphadenopathy; Pancreas; tuberculosis |
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NLM (Medline) |
01259326 |
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35027493 |
Article |
Q3 |
321 |
14162 |
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132 |
Rajabto W., Priantono D., Mulyadi R. |
36519576100;57219443427;56403164500; |
Pulmonary Embolism in Hospitalized Patient with Coronavirus Disease 2019 (COVID-19) |
2021 |
Acta medica Indonesiana |
53 |
4 |
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493 |
496 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123461861&partnerID=40&md5=389908dacd77633e6cd2254227617a8c |
Division of Hematology-Medical Oncology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo General HospitalJakarta, Indonesia |
Rajabto, W., Division of Hematology-Medical Oncology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo General HospitalJakarta, Indonesia; Priantono, D.; Mulyadi, R. |
Coronavirus disease 2019 (COVID-19) has been a global pandemic for over a year. Meanwhile, thrombosis occurs in up to one-third of hospitalized patients with the disease, while pulmonary embolism has been reported to be the most dangerous thrombosis which greatly increases mortality in COVID-19.Hospitalized patients with COVID-19 are at high risk of thromboembolic complications such as deep vein thrombosis and pulmonary embolism. The hypercoagulable state caused by COVID-19 leads to activation of coagulation cascade, meanwhile, CT pulmonary angiography is used to diagnose or exclude pulmonary embolism. Furthermore, ground-glass opacities are also evaluated using this modality. Low molecular weight heparin is the anticoagulant of choice due to simplicity in administration and low risk of drug-drug interactions.Pulmonary embolism occurs in COVID-19 patients without DVT. Based on the results, parenteral anticoagulant followed by DOAC is the mainstay of treatment in COVID-19 coagulopathy. |
COVID-19; pulmonary embolism; thrombosis |
anticoagulant agent; complication; diagnostic imaging; human; lung embolism; vein thrombosis; virology; Anticoagulants; COVID-19; Humans; Pulmonary Embolism; SARS-CoV-2; Venous Thrombosis |
NLM (Medline) |
01259326 |
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35027499 |
Article |
Q3 |
321 |
14162 |
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133 |
Shatri H., Prasetyaningtyas A., Putranto R., Rinaldi I. |
28767986500;57426347900;56074051000;23475122400; |
Palliative Prognostic Index Validation in Hospitalized Advanced Cancer Patients in Indonesia Tertiary Hospitals |
2021 |
Acta medica Indonesiana |
53 |
4 |
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442 |
449 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123460876&partnerID=40&md5=6a2a641fbdfa3aa8f7eb17fb4fc4458e |
1. Division of Psychosomatic and Pallliative, Indonesia. 2. Clinical Epidemiology Unit, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - dr. Cipto Mangunkusumo HospitalJakarta, Indonesia |
Shatri, H., 1. Division of Psychosomatic and Pallliative, Indonesia. 2. Clinical Epidemiology Unit, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - dr. Cipto Mangunkusumo HospitalJakarta, Indonesia; Prasetyaningtyas, A.; Putranto, R.; Rinaldi, I. |
BACKGROUND: Accurate prediction of survival is important for advanced cancer patients to determine medical interventions plan the patient's lives and prepare for their death. The palliative prognostic index (PPI) is most popular scores used worldwide to predict life expectancy in advanced cancer palliative patients. The purpose of this study was to test validity and the performance of PPI in Cipto Mangunkusumo Hospital as a Tertiary Referral Nasional Hospital. METHODS: This retrospective cohort study, uses total subject during study with consecutive sampling. Palliative prognostic index was assessed by a palliative care team (PCT). Demographic data were summarized as n (%) and Chi square for categorical variables and median or mean for continuous variables. Overall survival was calculated using the Kaplan-Meier method with hazard ratios. The performance of PPI analyzed using SPSS version 20.0, includes for Receiving Operator Characteristics (ROC) and Hosmer-Lemeshow calibration test. RESULTS: 160 patients were included in the PPI study. The subjects have an average age of 50.08 years and are mostly women 68.10%. 28 (17.50%) had symptoms of dyspnoea, 22 (14.60%) pneumonia, and 19 (11.90%) had pain. The number of patients who died during hospitalisation was 83 (51.90%). PPI sum score >6 109 (68,10%). Calibration performance PPI score reached x2 = 8.915 (p = 0.259), and showed correlation r 0.799 (p 0.000). The accuracy of PPI scores in predicting survival in advanced cancer patients in studies for survival <3 weeks 81%, with a sensitivity of 85%, specificity 70%, PPV 86%, and NPV 67%. Predictive accuracy of survival within 3-6 weeks had 76%, sensitivity 66%, specificity 88%, PPV 85% and NPV 70%. PPI score discrimination performance is had a AUC value of 0.822 (95% CI 0.749-0.895). CONCLUSION: Palliative Prognostic Index (PPI ) is valid and has good performance in predicting the survival of advanced cancer patients and may be used to help clinicians in palliative care consultation. |
Cancer; palliative prognostic index (PPI); tertiary hospital; validation |
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NLM (Medline) |
01259326 |
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35027491 |
Article |
Q3 |
321 |
14162 |
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134 |
Gamalliel N., Sutanto R.L., Wardhani A.N.H. |
57222183873;57222060277;57409481900; |
To involve or not to involve: youth participation in Indonesia's pandemic campaign |
2021 |
The Lancet Regional Health - Western Pacific |
15 |
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100290 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122707972&doi=10.1016%2fj.lanwpc.2021.100290&partnerID=40&md5=fd7cc1adaa04029c8ef04d93e3a955e5 |
National COVID-19 Volunteer, Indonesian Medical Students’ Executive Boards Association, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta, Indonesia |
Gamalliel, N., National COVID-19 Volunteer, Indonesian Medical Students’ Executive Boards Association, Jakarta, Indonesia, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Sutanto, R.L., National COVID-19 Volunteer, Indonesian Medical Students’ Executive Boards Association, Jakarta, Indonesia, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Wardhani, A.N.H., National COVID-19 Volunteer, Indonesian Medical Students’ Executive Boards Association, Jakarta, Indonesia, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta, Indonesia |
[No abstract available] |
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Elsevier Ltd |
26666065 |
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Note |
#N/A |
#N/A |
#N/A |
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135 |
Pujianto D., Permatasari S. |
8745734300;57408686700; |
Mouse CD52 is predominantly expressed in the cauda epididymis, regulated by androgen and lumicrine factors |
2021 |
Journal of Human Reproductive Sciences |
14 |
4 |
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350 |
355 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122693572&doi=10.4103%2fjhrs.jhrs_29_21&partnerID=40&md5=442cc066428b3f1038737d1f1d81f1cc |
Department of Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, 10430, Indonesia; Department of Biology, Faculty of Medicine, Universitas Palangka Raya, Indonesia |
Pujianto, D., Department of Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, 10430, Indonesia; Permatasari, S., Department of Biology, Faculty of Medicine, Universitas Palangka Raya, Indonesia |
Background: Sperm maturation takes place through contact between sperm and proteins produced in the epididymal lumen. CD52 had been characterised in the sperm; however, the expression and its regulation in the epididymis are mostly unknown. Aim: This study aimed to analyse the expression and regulation of CD52 in the mouse epididymis. Setting and Design: Experimental design was used in this study. Materials and Methods: Epididymis tissues from mice strain Deutch Democratic Yokohama were used as sources of total RNA. Bioinformatic tool was used to predict signal peptides. Quantitative real-Time reverse transcription-polymerase chain reaction was used to analyse tissue distribution, androgen, testicular factors dependency and postnatal development. Statistical Analysis: One-way analysis of variance was used to analyse differences between treatment and control untreated group. P < 0.05 was determined as a significant difference. Results: CD52 amino acid sequence contains a signal peptide, indicating it is a secretory protein. CD52 exhibited region-specific expression in the epididymis, with the highest level being in the cauda. CD52 expression was regulated by androgen indicated by a significant downregulation at day 1 and day 3 following a castration (P < 0.05). Dependency on androgen was confirmed by injection of exogenous testosterone which prevented downregulation by 50%. Moreover, lumicrine factors also influenced CD52 expression indicated by ligation of efferent duct which also reduced expression at day 1 to day 5 following the ligation (P < 0.05). CD52 expression was developmentally regulated. This was shown by increase in the level of expression starting at day 15 postnatally. Conclusion: CD52 shows characteristics of genes involved in sperm maturation in the epididymis. © 2021 Wolters Kluwer Medknow Publications. All rights reserved. |
Androgen; CD52; Epididymis; Lumicrine; Sperm maturation |
androgen; bromethol; CD52 antigen; hormone; lumicrine; RNA; signal peptide; testosterone; unclassified drug; amino acid sequence; androgen therapy; animal experiment; animal tissue; antigen expression; Article; castration; cauda epididymis; controlled study; down regulation; epididymis; male; mouse; nonhuman; postnatal development; quantitative analysis; real time reverse transcription polymerase chain reaction; spermatozoon maturation; tissue distribution |
Wolters Kluwer Medknow Publications |
09741208 |
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Article |
Q3 |
484 |
10359 |
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136 |
Daulay R.S., Saragih R.A.C., Daulay R.M., Ganie R.A., Tann G., Supriyatno B. |
57201677329;57204321772;6504644320;57193788722;57213061716;37068046400; |
Role of interferon-gamma +874 a/t single-nucleotide polymorphism and tuberculosis susceptibility of pediatric population in north sumatera, indonesia |
2021 |
Open Access Macedonian Journal of Medical Sciences |
9 |
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1057 |
1060 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121721756&doi=10.3889%2foamjms.2021.7441&partnerID=40&md5=490f692392638585ec48409e7140509b |
Department of Child Health, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Department of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Department of Child Health, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Daulay, R.S., Department of Child Health, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Saragih, R.A.C., Department of Child Health, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Daulay, R.M., Department of Child Health, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Ganie, R.A., Department of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Tann, G., Department of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Supriyatno, B., Department of Child Health, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
BACKGROUND: Tuberculosis (TB) remains to be a leading cause of morbidity and mortality worldwide. The immune defense against Mycobacterium tuberculosis is complicated. Interferon-gamma (IFN-γ) is the main cytokine involved in the immune response of TB. To date, the role of +874 A/T single-nucleotide polymorphism (SNP) and TB disease susceptibility continues to be controversial. AIM: The aim of this study was to investigate the role of +874 A/T SNP and TB disease susceptibility of pediatric population in North Sumatera, Indonesia. METHODS: A case–control study was conducted in Medan and Batubara, North Sumatera, Indonesia, from January to December 2016. A total of 51 children with TB and 51 healthy controls were enrolled in this study. Subjects were 2 months–14 years old age children diagnosed with TB and written informed consent from the parents or the caregivers to participate. Subjects were withdrawn from the study when immunodeficiency condition was found or suffered from other infection disease. DNA samples were obtained from all of the subjects. +874 A/T SNP was identified by performing the amplification refractory mutational system-polymerase chain reaction method. IFN-γ levels were measured using human enzyme-linked immunosorbent assay. Data analysis was performed using Chi-square and Mann–Whitney test. P < 0.05 was considered statistically significant. RESULTS: The result of this study reveals that the presence of AA, AT, and TT genotype in TB patients was 31 (60.8%), 20 (39.2%), and 0 (0%), respectively (p = 0.023). Significant decreased production of IFN-γ levels (p = 0.042) was found in TB patients 9.41 (1.10–28.06) pg/ml. CONCLUSION: Our study demonstrated significant evidence of the role of +874 A/T SNP and TB disease susceptibility of pediatric population in North Sumatera, Indonesia, predominantly AA genotype. Significant decreased production of IFN-γ reported among pediatric TB. © 2021 Rini Savitri Daulay, Rina Amalia C. Saragih, Ridwan Muchtar Daulay, Ratna Akbari Ganie, Gino Tann, Bambang Supriyatno. |
+874 A/T; Indonesia; Interferon-gamma; Pediatric; Single-nucleotide polymorphism; Tuberculosis |
gamma interferon; adolescent; amplification refractory mutation system polymerase chain reaction; Article; case control study; child; controlled study; cytokine production; disease predisposition; DNA isolation; enzyme linked immunosorbent assay; female; fine needle aspiration biopsy; gene frequency; genotype; human; immune deficiency; infant; major clinical study; male; newborn; preschool child; school child; single nucleotide polymorphism; thorax radiography; tuberculin test; tuberculosis |
Scientific Foundation SPIROSKI |
18579655 |
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Article |
Q3 |
288 |
15252 |
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137 |
Aditianingsih D., Hidayat J., Ginting V.M. |
56312263600;57221444286;57377973500; |
Comparison of bioimpedance versus pulse contour analysis for intraoperative cardiac index monitoring in patients undergoing kidney transplantation |
2021 |
Anesthesiology and Pain Medicine |
11 |
5 |
e117918 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121465922&doi=10.5812%2fAAPM.117918&partnerID=40&md5=cc44c95f4c7c6f8cc3dd0d4b279457ff |
Department of Anesthesiology and Intensive Therapy, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia |
Aditianingsih, D., Department of Anesthesiology and Intensive Therapy, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Hidayat, J., Department of Anesthesiology and Intensive Therapy, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Ginting, V.M., Department of Anesthesiology and Intensive Therapy, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia |
Background: Cardiac index (CI; cardiac output indexed to body surface area) is routinely measured during kidney transplant surgery. Bioimpedance cardiometry is a transthoracic impedance as the non-invasive alternative for hemodynamic monitoring, using semi-invasive uncalibrated pulse wave or contour (UPC) analysis. Objectives: We performed a cross-sectional observational study on 50 kidney transplant patients to compare the CI measurement agreement, concordance rate, and trending ability between bioimpedance and UPC analysis. Methods: For each patient, CI was measured by bioimpedance analysis (ICON™) and UPC analysis (EV1000™) devices at three time points: after induction, during incision, and at reperfusion. The device measurement accuracy was assessed by the bias value, limit of agreement (LoA), and percentage error (PE) using Bland-Altman analyses. Trending ability was assessed by angular bias and polar concordance through four-quadrant and polar plot analyses. Results: From each time point and pooled measurement, the correlation coefficients were 0.267, 0.327, 0.321, and 0.348. BlandAltman analyses showed mean bias values of 1.18, 1.06, 1.48, and 1.30, LoA of-1.35 to 3.72,-1.39 to 3.51,-1.07 to 4.04, and-1.17 to 3.78, and PE of 82.21, 78.50, 68.74, and 74.58%, respectively. Polar plot analyses revealed angular bias values of-10.37º,-15.01º,-18.68º, and-12.62º, with radial LoA of 89.79º, 85.86º, 83.38º, and 87.82º, respectively. The four-quadrant plot concordance rates were 70.77, 67.35, 65.90, and 69.79%. These analyses showed poor agreement, weak concordance, and low trending ability of bioimpedance cardiometry to UPC analysis. Conclusions: Bioimpedance and UPC analysis for CI measurements were not interchangeable in patients undergoing kidney transplant surgery. Cardiac index monitoring using bioimpedance cardiometry during kidney transplantation should be interpreted cautiously because it showed poor reliability due to low accuracy, precision, and trending ability for CI measurement. © 2021, Author(s). |
Cardiac Output; Intraoperative Monitoring; Kidney Transplantation; Pulse Wave Analysis; Transthoracic Impedance |
atracurium besilate; dobutamine; fentanyl; noradrenalin; propofol; sevoflurane; adult; aged; agitation; anesthesia induction; arterial pressure; Article; body mass; bradycardia; cardiac index; cardiopulmonary bypass; central venous pressure; chronic kidney failure; correlation coefficient; cross-sectional study; female; heart arrhythmia; heart output; heart rate; heart stroke volume; hemodynamic monitoring; human; hypertension; intraoperative monitoring; kidney transplantation; major clinical study; male; mean arterial pressure; measurement accuracy; nausea and vomiting; observational study; outcome assessment; pleura effusion; prospective cost; prospective study; pulse oximetry; pulse wave; systolic blood pressure; tachycardia; transesophageal echocardiography; uncalibrated pulse contour |
Kowsar Medical Institute |
22287523 |
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Article |
Q2 |
438 |
11251 |
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138 |
Gunardi T.H., Susantono D.P., Victor A.A., Sitompul R. |
57195939686;57353411000;57191055282;8312163900; |
Atopobiosis and dysbiosis in ocular diseases: Is fecal microbiota transplant and probiotics a promising solution? |
2021 |
Journal of Ophthalmic and Vision Research |
16 |
4 |
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631 |
643 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119915775&doi=10.18502%2fJOVR.V16I4.9754&partnerID=40&md5=8d0e2b295a398c85ff1c28564276103b |
Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Ophthalmology, Dr. Cipto Mangunkusumo National General Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Gunardi, T.H., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Susantono, D.P., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Victor, A.A., Department of Ophthalmology, Dr. Cipto Mangunkusumo National General Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Sitompul, R., Department of Ophthalmology, Dr. Cipto Mangunkusumo National General Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Purpose: To highlight the role of atopobiosis and dysbiosis in the pathomechanism of autoimmune uveitis, therefore supporting fecal microbiota transplant (FMT) and probiotics as potential targeted-treatment for uveitis. Methods: This review synthesized literatures upon the relation between gut microbiota, autoimmune uveitis, FMT, and probiotics, published from January 2001 to March 2021 and indexed in PubMed, Google Scholar, CrossRef. Results: The basis of the gut-eye axis revolves around occurrences of molecular mimicry, increase in pro-inflammatory cytokines, gut epithelial barrier disruption, and translocation of microbes to distant sites. In patients with autoimmune uveitis, an increase of gut Fusobacterium and Enterobacterium were found. With current knowledge of aforementioned mechanisms, studies modifying the gut microbiome and restoring the physiologic gut barrier has been the main focus for pathomechanism-based therapy. In mice models, FMT and probiotics targeting repopulation of gut microbiota has shown significant improvement in clinical manifestations of uveitis. Consequently, a better understanding in the homeostasis of gut microbiome along with their role in the gut-eye axis is needed to develop practical targeted treatment. Conclusion: Current preliminary studies are promising in establishing a causative gut-eye axis relationship and the possibility of conducting FMT and probiotics as targeted treatment to mitigate autoimmune uveitis, to shorten disease duration, and to prevent further complications. © 2021 GUNARDI ET AL. THIS IS AN OPEN ACCESS ARTICLE DISTRIBUTED UNDER THE CREATIVE COMMONS ATTRIBUTION LICENSE | PUBLISHED BY KNOWLEDGE E |
Atopobiosis; Autoimmune; Dysbiosis; Gut-Eye Axis; Uveitis |
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Knowledge E |
20082010 |
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Review |
Q3 |
557 |
9058 |
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139 |
Harzif A., Anggraeni T., Syaharutsa D., Hellyanti T. |
57191493435;57192894826;57204145265;57217993236; |
Hysteroscopy role for female genital tuberculosis |
2021 |
Gynecology and Minimally Invasive Therapy |
10 |
4 |
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243 |
246 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119285063&doi=10.4103%2fGMIT.GMIT_151_20&partnerID=40&md5=2dea6cbfe3f4a65bb9bc6b632d3cc573 |
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Department of Anatomical Pathology, Faculty of Medicine, University of Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia |
Harzif, A., Department of Obstetrics and Gynecology, Faculty of Medicine, University of Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Anggraeni, T., Department of Obstetrics and Gynecology, Faculty of Medicine, University of Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Syaharutsa, D., Department of Obstetrics and Gynecology, Faculty of Medicine, University of Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Hellyanti, T., Department of Anatomical Pathology, Faculty of Medicine, University of Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia |
Female genital tuberculosis affects the quality of women's lives. One of the symptoms is amenorrhea. In our country, it is still underdiagnosed due to limited resources. Hysteroscopy is known as one of the diagnostic tools for this condition. We performed hysteroscopy and endometrial biopsy in four cases. Hysteroscopy findings show various signs. Histopathological examination showed typical features of tuberculosis in some cases. We also learned that hysteroscopy could evaluate the condition of the endometrium when ongoing and after treatment is accomplished. It is useful for further explanation to the client. Hysteroscopy can be utilized as a diagnostic tool for endometrial sampling, evaluate intracavity condition after treatment, and prognostic tool for future reproductive function. © 2021 Wolters Kluwer Medknow Publications. All rights reserved. |
Amenorrhea; caseous necrosis; genital tuberculosis; hysteroscopy |
tuberculostatic agent; adolescent; adult; Article; case report; caseation; chronic inflammation; clinical article; clinical feature; diagnostic value; echography; endometritis; endometrium; endometrium biopsy; female; female genital tuberculosis; granulomatous inflammation; histopathology; human; human tissue; hysteroscopy; laparotomy; primary amenorrhea; secondary amenorrhea; sister; tuberculous peritonitis; tuberculous spondylitis |
Wolters Kluwer Medknow Publications |
22133070 |
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Article |
Q3 |
441 |
11202 |
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140 |
Waters S., Agostino M., Lee S., Ariyanto I., Kresoje N., Leary S., Munyard K., Gaudieri S., Gaff J., Irish A., Keil A.D., Price P., Allcock R.J.N. |
57195514207;34771068500;56272877300;57193538110;55413004800;56906725500;6506851062;56245970200;57193534280;7004314741;57339623900;57201814264;7003764659; |
Sequencing directly from clinical specimens reveals genetic variations in HCMV-encoded chemokine receptor us28 that may influence antibody levels and interactions with human chemokines |
2021 |
Microbiology Spectrum |
9 |
2 |
e00020-21 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119150219&doi=10.1128%2fSpectrum.00020-21&partnerID=40&md5=d943d30d66f9a7de80628f87fcbb3810 |
Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia; Curtin Institute for Computation, Curtin University, Bentley, WA, Australia; Department of Microbiology, PathWest Laboratory Medicine WA, Nedlands, WA, Australia; Virology and Cancer Pathobiology Research Center, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; School of Biomedical Sciences, University of Western Australia, Perth, WA, Australia; Institute for Immunology and Infectious Diseases, Murdoch University, Murdoch, WA, Australia; School of Human Sciences, University of Western Australia, Perth, WA, Australia; Department of Medicine, Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, United States; Department of Nephrology, Fiona Stanley Hospital, Murdoch, WA, Australia; Department of Diagnostic Genomics, PathWest Laboratory Medicine WA, Nedlands, WA, Australia |
Waters, S., Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia; Agostino, M., Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia, Curtin Institute for Computation, Curtin University, Bentley, WA, Australia; Lee, S., Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia, Department of Microbiology, PathWest Laboratory Medicine WA, Nedlands, WA, Australia; Ariyanto, I., Virology and Cancer Pathobiology Research Center, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Kresoje, N., School of Biomedical Sciences, University of Western Australia, Perth, WA, Australia; Leary, S., Institute for Immunology and Infectious Diseases, Murdoch University, Murdoch, WA, Australia; Munyard, K., Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia; Gaudieri, S., Institute for Immunology and Infectious Diseases, Murdoch University, Murdoch, WA, Australia, School of Human Sciences, University of Western Australia, Perth, WA, Australia, Department of Medicine, Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, United States; Gaff, J., Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia; Irish, A., Department of Nephrology, Fiona Stanley Hospital, Murdoch, WA, Australia; Keil, A.D., Department of Microbiology, PathWest Laboratory Medicine WA, Nedlands, WA, Australia; Price, P., Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA, Australia; Allcock, R.J.N., School of Biomedical Sciences, University of Western Australia, Perth, WA, Australia, Department of Diagnostic Genomics, PathWest Laboratory Medicine WA, Nedlands, WA, Australia |
Human cytomegalovirus (HCMV) is a beta-herpesvirus carried by;80% of the world’s population. Acute infections are asymptomatic in healthy individuals but generate diverse syndromes in neonates, solid organ transplant recipients, and HIV-infected individuals. The HCMV gene US28 encodes a homolog of a human chemokine receptor that is able to bind several chemokines and HIV gp120. Deep sequencing technologies were used to sequence US28 directly from 60 clinical samples from Indonesian HIV patients and Australian renal transplant recipients, healthy adults, and neonates. Molecular modeling approaches were used to predict whether nine nonsynonymous mutations in US28 may alter protein binding to a panel of six chemokines and two variants of HIV gp120. Ninety-two percent of samples contained more than one variant of HCMV, as defined by at least one nonsynonymous mutation. Carriage of these variants differed between neonates and adults, Australian and Indonesian samples, and saliva samples and blood leukocytes. Two nonsynonymous mutations (N170D and R267K) were associated with increased levels of immediate early protein 1 (IE-1) and glycoprotein B (gB) HCMV-reactive antibodies, suggesting a higher viral burden. Seven of the nine mutations were predicted to alter binding of at least one ligand. Overall, HCMV variants are common in all populations and have the potential to affect US28 interactions with human chemokines and/or gp120 and alter responses to the virus. The findings relied on deep sequencing technologies applied directly to clinical samples, so the variants exist in vivo. IMPORTANCE Human cytomegalovirus (HCMV) is a common viral pathogen of solid organ transplant recipients, neonates, and HIV-infected individuals. HCMV encodes homologs of several host genes with the potential to influence viral persistence and/ or pathogenesis. Here, we present deep sequencing of an HCMV chemokine receptor homolog, US28, acquired directly from clinical specimens. Carriage of these variants differed between patient groups and was associated with different levels of circulating HCMV-reactive antibodies. These features are consistent with a role for US28 in HCMV persistence and pathogenesis. This was supported by in silico analyses of the variant sequences demonstrating altered ligand-binding profiles. The data delineate a novel approach to understanding the pathogenesis of HCMV and may impact the development of an effective vaccine. Copyright © 2021 Waters et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
Chemokine receptor; Deep sequencing; HIV patients; Human cytomegalovirus; Renal transplant recipients; US28 |
arginine; asparagine; aspartic acid; chemokine; chemokine receptor; chemokine receptor US28; glycoprotein B; glycoprotein gp 120; immediate early protein; immediate early protein 1; lysine; unclassified drug; virus antibody; chemokine; chemokine receptor; protein binding; US28 receptor, Cytomegalovirus; viral protein; virus antibody; adult; Article; Australian; blood; controlled study; genetic variation; graft recipient; human; Human cytomegalovirus; Human immunodeficiency virus infection; Indonesian; kidney transplantation; leukocyte; molecular model; mutation; newborn; nonhuman; protein protein interaction; saliva; virus load; amino acid sequence; blood; Cytomegalovirus; cytomegalovirus infection; genetic variation; genetics; high throughput sequencing; immunology; infant; isolation and |
American Society for Microbiology |
21650497 |
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34704798 |
Article |
Q1 |
2502 |
907 |
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