747 |
Rahyussalim A.J., Sahputra R.E., Yanwirasti, Manjas M., Whulanza Y., Kurniawati T., Aprilya D., Zufar M.L.L. |
55212166100;57212460235;56711920400;24473177100;36807053500;55213290600;57193710642;57216349850; |
The effect of mesenchymal stem cell-enriched scaffolds on mmp-8 and tgf-β levels of vertebrae postlaminoplasty in rabbit model |
2021 |
Stem Cells and Cloning: Advances and Applications |
14 |
|
|
27 |
37 |
|
|
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111052490&doi=10.2147%2fSCCAA.S314107&partnerID=40&md5=6b23d4e3b167216975366ce0f5f83ae6 |
Department of Orthopedics and Traumatology Clinics, Faculty of Medicine, Universitas of Indonesia-Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Stem Cell and Tissue Engineering Cluster, IMERI Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Department of Surgery, Faculty of Medicine, Universitas Andalas-RSUP M. Djamil, Padang, Indonesia; Department of Anatomy, Faculty of Medicine, Universitas Andalas-RSUP M. Djamil, Padang, Indonesia; Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Jakarta, Indonesia |
Rahyussalim, A.J., Department of Orthopedics and Traumatology Clinics, Faculty of Medicine, Universitas of Indonesia-Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Stem Cell and Tissue Engineering Cluster, IMERI Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Sahputra, R.E., Department of Surgery, Faculty of Medicine, Universitas Andalas-RSUP M. Djamil, Padang, Indonesia; Yanwirasti, Department of Anatomy, Faculty of Medicine, Universitas Andalas-RSUP M. Djamil, Padang, Indonesia; Manjas, M., Department of Surgery, Faculty of Medicine, Universitas Andalas-RSUP M. Djamil, Padang, Indonesia; Whulanza, Y., Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Jakarta, Indonesia; Kurniawati, T., Stem Cell and Tissue Engineering Cluster, IMERI Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Aprilya, D., Department of Orthopedics and Traumatology Clinics, Faculty of Medicine, Universitas of Indonesia-Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Zufar, M.L.L., Department of Orthopedics and Traumatology Clinics, Faculty of Medicine, Universitas of Indonesia-Cipto Mangunkusumo General Hospital, Jakarta, Indonesia |
Introduction: Some laminoplasty procedures still have restenosis because of bony-bridging failure of the laminar hinge. The present study aimed to determine the effect of mesenchymal stem cell (MSC)-enriched scaffolds on vertebral regeneration after laminoplasty on the basis of the number of osteoblasts, matrix metalloproteinase-8 (MMP-8), and transforming growth factor-beta (TGF-β) levels. Methods: Laminoplasty procedure using the Hirabayashi technique was conducted at the lumbar level in 32 rabbits that were divided into four and three groups of the control (C) and treatment groups, respectively, with different types of laminoplasty spacer (T1, autograft; T2, scaffold; and T3, scaffold with MSCs). Histopathological studies were conducted to calculate the number of osteoblasts and enzyme-linked immunosorbent assay tests to detect MMP-8 and TGF-β 4 weeks after the surgery. Results: The results showed a significant decrease in MMP-8 level in the T3 group compared with that in the control group (p < 0.05). A significant difference exists between the average number of newly formed osteoblasts in the control group compared with that in the T3 group (p < 0.05) with a higher mean blood TGF-β level of all experimental groups compared with that of the control group (p = 0.58). Conclusion: The significant decrease in MMP-8 levels, increase in TGF-β levels, and increased number of osteoblasts on MSC-seeded polylactic acid scaffolds could be useful to support the laminoplasty procedure to prevent restenosis because it was biocompatible and promoted the bone healing process. © 2021 Rahyussalim et al. |
Hirabayashi technique; Laminoplasty; Mesenchymal stem cells; Scaffolds |
enrofloxacin; eosin; hematoxylin; ketoprofen; liquid nitrogen; neutrophil collagenase; polylactic acid; transforming growth factor beta; animal experiment; animal model; animal tissue; Article; autograft; blood sampling; body weight; bone development; cell suspension; cell viability; cervical spine dislocation; controlled study; cryopreservation; enzyme linked immunosorbent assay; follow up; fracture healing; histopathology; inflammation; injury; laminoplasty; mesenchymal stem cell; neoplasm; nonhuman; nutrition; ossification; osteoblast; restenosis; sample size; spine; umbilical cord |
Dove Medical Press Ltd |
11786957 |
|
|
Article |
Q2 |
606 |
8341 |
|
|