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No records
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746 |
Schuwirth L., Findyartini A. |
7003825152;56543777300; |
Never waste a good crisis: Resilient health professions education |
2021 |
Asia Pacific Scholar |
6 |
3 |
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1 |
4 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111073193&doi=10.29060%2fTAPS.2021-6-3%2fEV6N3&partnerID=40&md5=9a31005ce32498af7be04ec902845ab8 |
Prideaux Centre for Research in Health Professions Education, College of Medicine and Public Health, Flinders University, Australia; Department of Medical Education, Faculty of Medicine, Universitas Indonesia, Indonesia |
Schuwirth, L., Prideaux Centre for Research in Health Professions Education, College of Medicine and Public Health, Flinders University, Australia; Findyartini, A., Department of Medical Education, Faculty of Medicine, Universitas Indonesia, Indonesia |
[No abstract available] |
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National University of Singapore, Faculty of Law |
24249335 |
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Editorial |
#N/A |
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813 |
Azmi L., Rukmana A., Sjatha F. |
57223235228;35491487100;55372815000; |
Cloning of the pe11 (LipX, Rv1169c) gene of a mycobacterium tuberculosis beijing strain into the pcDNA3.1 plasmid vector |
2021 |
Makara Journal of Science |
25 |
1 |
6 |
35 |
42 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105345058&doi=10.7454%2fmss.v25i1.1206&partnerID=40&md5=15f89692c545a91386310b30b135be6b |
Biomedical Student, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Departement of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, 10320, Indonesia |
Azmi, L., Biomedical Student, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Rukmana, A., Departement of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, 10320, Indonesia; Sjatha, F., Departement of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, 10320, Indonesia |
Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. It is a persistent global health problem with a high mortality rate. Currently, TB is controlled by administering the Bacillus Calmette-Guerin (BCG) vaccine, but the effectiveness of its protection varies among individuals in a population. The pe/ppe gene family comprises a typical group of genes that play a role in avoiding the host immune response and inducing persistent TB infection. Based on in silico analysis, the pe11 gene has estimated immunogenicity and potential as a TB seed vaccine candidate. The pe11 gene from an Indonesian isolate of an M. tuberculosis Beijing strain was amplified by polymerase chain reaction (PCR) and inserted into the mammalian expression vector pcDNA3.1. The recombinant vector pcDNA3.1-pe11 was used to transform Top10 competent Escherichia coli. Clones from the transformation were subjected to colony PCR to confirm the direction of the insert. Sequencing was performed to confirm the correctness of the insert sequence. In this study, the pe11 gene was successfully cloned into the pcDNA3.1 vector in the correct direction to assure PE11 expression. No mutations were found in the pe11 gene insert, compared with the M. tuberculosis H37Rv sequence as the standard. A pcDNA3.1 vector contain-ing the pe11 gene derived from an M. tuberculosis Beijing strain was successfully constructed. © 2021, Universitas Indonesia. All rights reserved. |
M. tuberculosis; PcDNA3.1; Pe11; Tuberculosis |
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Universitas Indonesia |
23391995 |
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Article |
#N/A |
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825 |
Wardoyo S., Pardede J.P.P., Asari H.A. |
57211106608;57222900416;57222898312; |
Patients’ characteristics following reoperation after modified blalock-taussig shunt (Mbts) in cardiac centre national general hospital cipto mangunkusumo from 2018-2020 |
2021 |
Bali Medical Journal |
10 |
1 |
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103 |
107 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104181375&doi=10.15562%2fbmj.v10i1.2124&partnerID=40&md5=71f0e48c1ff1cce6ec58341517f2fb6a |
Thoracic, Cardiac, and Vascular Surgery Division, Department of Surgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia |
Wardoyo, S., Thoracic, Cardiac, and Vascular Surgery Division, Department of Surgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia; Pardede, J.P.P., Thoracic, Cardiac, and Vascular Surgery Division, Department of Surgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia; Asari, H.A., Thoracic, Cardiac, and Vascular Surgery Division, Department of Surgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia |
Background: Modified Blalock-Taussig shunt (MBTS) is a common palliative procedure for congenital heart defect to connect the systemic to the pulmonary circulation via a synthetic shunt from a subclavian artery to a pulmonary artery. However, certain postoperative complications do exist, such as blockage. Early identification of modifiable risk factors is important to prompt early intervention for better outcomes. Material: This article is a descriptive narrative retrospective study. We gather data from the Cipto Mangunkusumo Hospital Cardiovascular Center medical report of 8 patients having undergone reoperation post-MBTS surgery as their first operation between January 2018 and October 2020. Data were analyzed using SPSS version 20 for Windows. Results: The patient’s age ranged from 3 months to 30 years old. Seven of them suffered from shunt occlusion complications, while the other suffered from the bleeding problem. The laboratory results showed that preoperative hemoglobin (18.9±4.1 g/dL vs. 16.90 (10.80-19.20) g/dL), hematocrit (58.8±12.40% vs. 42.9±14.30%), platelet counts (284.487±147.003 vs. 210.625±104.688) 103/uL, and oxygen saturation (86.00 (75.00-89.00) vs. 70.00±17.00) levels were higher than the pre-reoperative. The coagulation markers showed that PT value has increasing trend (1.10 (0.90-2.30) vs. 1.30±0.20) while aPTT (5.90 (2.90-6.30) vs. 2.30 (1.60-5.30)) seconds and activated clotting time (ACT) (205.00±86.00 vs. 165.00 (114.00-255.00)) showed the opposite. Conclusion: MBTS remains the first-choice bridging palliation surgery to increase the pulmonary blood flow in congenital heart defects. The cause of shunt failure remains inconclusive in this study. Other studies suggest that graft material choice, S/PA ratio, S/W ratio, operative approach, thrombus formation, and aPTT value contribute to shunt failure, thus needing a reoperation. © 2021, Sanglah General Hospital. All rights reserved. |
Characteristic; Modified Blalock-Taussig Shunt; Re-operation |
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Sanglah General Hospital |
20891180 |
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Article |
#N/A |
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843 |
Nelson B., Sitohang I.B.S., Marissa M., Indriatmi W., Wisnu W. |
57222619613;56734569200;57210795916;57189888041;57200425452; |
A comparative study of melasma severity after hyperthyroid therapy in hyperthyroid subjects with melasma |
2021 |
Acta Dermatovenerologica Alpina, Pannonica et Adriatica |
30 |
1 |
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31 |
34 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103497112&doi=10.15570%2factaapa.2021.7&partnerID=40&md5=12823fac55643be95fa3cbe943888df6 |
Department of Dermatology and Venereology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Metabolic Endocrine Division, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
Nelson, B., Department of Dermatology and Venereology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Sitohang, I.B.S., Department of Dermatology and Venereology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Marissa, M., Department of Dermatology and Venereology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Indriatmi, W., Department of Dermatology and Venereology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Wisnu, W., Metabolic Endocrine Division, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
Introduction: Melasma, and its variant chloasma, is an acquired and chronic disorder of hyperpigmentation, characterized by symmetrical hypermelanoses of the face. The exact pathogenesis of melasma remains unclear. Several hormones are thought to play a role, including thyroid hormones. The study’s objectives are to determine the proportion of melasma cases in hyperthyroid patients and to compare the severity of melasma before and after medications of hyperthyroid therapy. Methods: A quasi-experimental (pre-post intervention) study was conducted in Jakarta from August 2019 to February 2020. Twenty-three patients either newly diagnosed with hyperthyroidism or that had undergone hyperthyroid therapy for a maximum of 3 months and also had melasma were recruited. The severity of melasma was scored with the modified Melasma Area and Severity Index (mMASI), and dermoscopy of the lesions was performed. The evaluation was performed after 3 months of hyperthyroid therapy. Results: Among the 69 hyperthyroid patients, 45 (65%) had melasma. The mean difference in the mMASI score was 0.49 (p > 0.05). Dermoscopy features did not show any differences between the start and end of the study. Conclusions: There is no significant improvement of melasma severity in hyperthyroid patients after 3 months of hyperthyroid therapy. © 2021, Slovene Medical Society. All rights reserved. |
Chloasma; Hyperthyroid; Melasma; MMASI |
retinol derivative; thiamazole; thyrotropin; adult; anticonvulsant therapy; Article; chloasma; clinical article; clinical examination; comparative study; disease severity; disease severity assessment; epiluminescence microscopy; euthyroidism; female; free thyroxine index; hormonal contraception; hormone substitution; human; hyperpigmentation; hyperthyroidism; melasma area and severity index; prospective study; scoring system; skin defect; telangiectasia; thyroid function; thyroxine blood level; face; hyperthyroidism; melanosis; treatment outcome; Face; Humans; Hyperthyroidism; Melanosis; Treatment Outcome |
Slovene Medical Society |
13184458 |
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33765755 |
Article |
#N/A |
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904 |
Widaty S., Soebono H., Sunarto, Emilia O. |
57208261546;6508242918;57221528270;6504322013; |
Development of a new instrument to assess clinical performance of residents in dermatology-Venereology department |
2021 |
Asia Pacific Scholar |
6 |
1 |
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70 |
82 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099335332&doi=10.29060%2fTAPS.2021-6-1%2fOA2241&partnerID=40&md5=7ccb3f67cc4a32f511df79fd292001b5 |
Department of Dermatology and Venereology, Faculty of Medicine, Universitas Indonesia – Dr. Cipto Mangunkusumo Hospital, Indonesia; Department of Dermatology and Venereology Faculty of Medicine, Universitas Gadjah Mada, Indonesia; Pediatric Department, Faculty of Medicine, Universitas Gadjah Mada Indonesia, Indonesia; Medical Education Department, Faculty of Medicine, Universitas Gadjah Mada, Indonesia |
Widaty, S., Department of Dermatology and Venereology, Faculty of Medicine, Universitas Indonesia – Dr. Cipto Mangunkusumo Hospital, Indonesia; Soebono, H., Department of Dermatology and Venereology Faculty of Medicine, Universitas Gadjah Mada, Indonesia; Sunarto, Pediatric Department, Faculty of Medicine, Universitas Gadjah Mada Indonesia, Indonesia; Emilia, O., Medical Education Department, Faculty of Medicine, Universitas Gadjah Mada, Indonesia |
Introduction: Performance assessment of residents should be achieved with evaluation procedures, informed by measured and current educational standards. The present study aimed to develop, test, and evaluate a psychometric instrument for evaluating clinical practice performance among Dermatology and Venereology (DV) residents. Methods: This is a qualitative and quantitative study conducted from 2014 to 2016. A pilot instrument was developed by 10 expert examiners from five universities to rate four video-recorded clinical performance, previously evaluated as good and bad performance. The next step was the application of the instrument to evaluate the residents which was carried out by the faculty of DV at two Universities. Results: The instrument comprised 11 components. There was a statistically significant difference (p < 0.001) between good and bad performance. Cronbach’s alpha documented high overall reliability (α = 0.96) and good internal consistency (α = 0.90) for each component. The new instrument correctly evaluated 95.0% of poor performance. The implementation study showed that inter-rater reliability between evaluators range from low to high (correlation coefficient α =0.79, p < 0.001). Conclusion: The instrument is a reliable and valid instrument for assessing clinical practice performance of DV residents. More studies are required to evaluate the instrument in different situation. © 2021 TAPS. All rights reserved. |
Clinical Assessment; Dermatology-Venereology; Instrument; Performance; Resident; Workplace-Based Assessment |
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National University of Singapore, Faculty of Law |
24249335 |
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Article |
#N/A |
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No records
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205 |
Krisanti E.A., Gofara T.Z., Rahyussalim A.J., Mulia K. |
14019920500;57260804700;55212166100;6507666535; |
Polyvinyl alcohol (PVA)/chitosan/sodium tripolyphosphate (STPP) hydrogel formulation with freeze-thaw method for anti-tuberculosis drugs extended release |
2021 |
AIP Conference Proceedings |
2370 |
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020010 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115001335&doi=10.1063%2f5.0063175&partnerID=40&md5=a3c9afce6e9d9f6305976a87eb3a4790 |
Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, 16424, Indonesia; Department of Orthopedic and Traumatology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, 10430, Indonesia |
Krisanti, E.A., Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, 16424, Indonesia; Gofara, T.Z., Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, 16424, Indonesia; Rahyussalim, A.J., Department of Orthopedic and Traumatology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, 10430, Indonesia; Mulia, K., Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, 16424, Indonesia |
Tuberculosis (TB) is one of the infectious diseases which must be routinely oral treated with anti-tuberculosis drugs performed 12-24 months. With treatment using drug implans that can release TB drugs in a longer time in the target location, it will be more effective, because the drug will be close to the target and go directly into the blood. In this study, the PVA / chitosan / STPP hydrogel formulation loaded with 4 types of anti-tuberculosis drugs (isoniazid, ethambutol, pirazinamide, and rifampicin) made using the freeze-thaw method. It is obtained that chitosan addition up until 20% could reduce drug's release rate and hold drug's release until 30 days, but the effect of STPP addition could not be seen because the ammount added is too small which is also shown from FTIR study that there is no STPP in the hydrogel detected. 80% PVA-20% Chitosan- 2% STPP hydrogel formulation release TB drugs the slowest and extended on Isoniazid, Ethambutol, and Rifampicin. SEM study shown that chitosan addition in PVA hydrogel resulted a homogen solution, and hydrogel with densely folded surface. 2% STPP addition resulted in smoother, more homogenous, and smaller pores morphology. © 2021 Author(s). |
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American Institute of Physics Inc. |
0094243X |
9780735441262 |
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Conference Paper |
- |
177 |
20880 |
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229 |
Nadhif M.H., Irsyad M., Rahyussalim A.J., Utomo M.S. |
57189057498;57220935587;55212166100;56180933900; |
Geometrical evaluation of CAM-configured thermoplastic polyurethane lattices for intervertebral disc replacements |
2021 |
AIP Conference Proceedings |
2382 |
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030006 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85114011637&doi=10.1063%2f5.0060049&partnerID=40&md5=894c1cbce6bdc985a2b86b40dcf80e06 |
Department of Medical Physics, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 6, DKI Jakarta, 10430, Indonesia; Medical Technology Cluster, Indonesia Medical Education and Research Institute (IMERI), Jl. Salemba Raya No. 6, DKI Jakarta, 10430, Indonesia; Department of Orthopedics and Traumatology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo Central Hospital, Jakarta, 10430, Indonesia; Stem Cell and Tissue Engineering Cluster, Indonesia Medical Education and Research Institute (IMERI), Jl. Salemba Raya No. 6, DKI Jakarta, 10430, Indonesia; Research Center for Metallurgy and Material, Indonesia Institute of Science (LIPI), Banten, 15310, Indonesia |
Nadhif, M.H., Department of Medical Physics, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 6, DKI Jakarta, 10430, Indonesia, Medical Technology Cluster, Indonesia Medical Education and Research Institute (IMERI), Jl. Salemba Raya No. 6, DKI Jakarta, 10430, Indonesia; Irsyad, M., Medical Technology Cluster, Indonesia Medical Education and Research Institute (IMERI), Jl. Salemba Raya No. 6, DKI Jakarta, 10430, Indonesia; Rahyussalim, A.J., Department of Orthopedics and Traumatology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo Central Hospital, Jakarta, 10430, Indonesia, Stem Cell and Tissue Engineering Cluster, Indonesia Medical Education and Research Institute (IMERI), Jl. Salemba Raya No. 6, DKI Jakarta, 10430, Indonesia; Utomo, M.S., Department of Medical Physics, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 6, DKI Jakarta, 10430, Indonesia, Medical Technology Cluster, Indonesia Medical Education and Research Institute (IMERI), Jl. Salemba Raya No. 6, DKI Jakarta, 10430, Indonesia, Research Center for Metallurgy and Material, Indonesia Institute of Science (LIPI), Banten, 15310, Indonesia |
Intervertebral discs (IVD) are prone to deformation due to higher stress that the discs can endure. Treatments for deformed IVDs include total disc replacements. Some studies concluded the superiority of spinal fusion compared to total disc replacement devices, either in the lumbar or cervical region. In current study, cuboid scaffolds made of thermoplastic polyurethane with lattice architecture were designed and configured using computer-aided manufacturing (CAM). The scaffolds were fabricated using fused filament fabrication. Process parameters were characterized and optimized to obtain scaffolds with uniform cells distribution. The struts at the top surface had average width values closer to the setpoints than the struts at the bottom surface, indicated by lower RMSE values for the struts at the top surface. However, the printing consistency in the same extrusion ratio at the bottom surface was higher than at the top surface, indicated by lower standard deviation values. Statistical analysis using standard deviation, RMSE, and Tukey's test showed that current scaffolds had non-uniform distribution between layers, which required further improvement. © 2021 Author(s). |
Computer-aided manufacturing; intervertebral disc; lattice structure; polyurethane |
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American Institute of Physics Inc. |
0094243X |
9780735441156 |
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Conference Paper |
- |
177 |
20880 |
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392 |
Hardiany N.S., Amaanullah M.Z.B., Antarianto R.D. |
57192910605;57224223597;57190862806; |
The effect of fasting on malondialdehyde level in liver and plasma of New Zealand white rabbits |
2021 |
AIP Conference Proceedings |
2353 |
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030093 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107282294&doi=10.1063%2f5.0052627&partnerID=40&md5=f1091bc64391ab80b12474af251808e8 |
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Indonesia; Center of Hypoxia and Oxidative Stress Studies, Department of Biochemistry and Molecular Biology, Faculty of Medicine Universitas Indonesia, Indonesia; Faculty of Medicine Universitas Indonesia, Indonesia; Departemnet of Histology, Faculty of Medicine Universitas Indonesia, Indonesia |
Hardiany, N.S., Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Indonesia, Center of Hypoxia and Oxidative Stress Studies, Department of Biochemistry and Molecular Biology, Faculty of Medicine Universitas Indonesia, Indonesia; Amaanullah, M.Z.B., Faculty of Medicine Universitas Indonesia, Indonesia; Antarianto, R.D., Departemnet of Histology, Faculty of Medicine Universitas Indonesia, Indonesia |
Oxidative stress is a state of imbalance of free radicals in the cells and is one of the causes of various diseases in humans. One method that is thought to reduce oxidative stress is calorie restriction or fasting. However, its effects remain unclear. This study was conducted to determine the effect of intermittent fasting and prolonged fasting on the levels of malondialdehyde (MDA) as an oxidative stress marker in the liver and plasma of New Zealand White rabbits. Fifteen of New Zealand White rabbits were divided into three groups (intermittent fasting (IF), prolonged fasting (PF), and control). MDA was measured in plasma and liver homogenate using spectrophotometry. The results were analyzed using One-way ANOVA test. The liver MDA level was decreased in the IF group, but not significant. However, there was a significant increase in plasma MDA levels both in the IF and PF groups. Moreover, liver MDA level was increased in PF group, although it was not significant. In conclusion, intermittent and prolonged fasting could increase plasma MDA levels significantly. © 2021 Author(s). |
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American Institute of Physics Inc. |
0094243X |
9780735440968 |
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Conference Paper |
- |
177 |
20880 |
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393 |
Yusra Y., Widjaja L., Witjaksono F., Timan I.S., Kumalawati J., Adiyanti S.S., Nurbaya S., Immanuel S. |
57220998367;56906852200;57070455800;6602793366;6504406695;57191952811;57225297244;12777341300; |
Amino acid profile in patients of chronic kidney disease on hemodialysis in Indonesia |
2021 |
AIP Conference Proceedings |
2353 |
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030014 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107266850&doi=10.1063%2f5.0052847&partnerID=40&md5=ca448315d7144ead78ebf8040fae2eb4 |
Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Department of Nutrition, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia |
Yusra, Y., Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Widjaja, L., Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Witjaksono, F., Department of Nutrition, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Timan, I.S., Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Kumalawati, J., Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Adiyanti, S.S., Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Nurbaya, S., Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia; Immanuel, S., Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia |
Protein energy wasting (PEW) is a nutritional disorder syndrome that occurs 28-80% in chronic kidney disease (CKD) patients on hemodialysis. Hemodialysis cause the nutrients loss including amino acids, increase protein catabolism induced by inflammation, and inhibit protein synthesis. The objective of this study was to acquire the amino acid profile in CKD patients on hemodialysis. This study used cross sectional design and involving 60 subjects of CKD patients aged >18 years on routine hemodialysis at Dr. Cipto Mangunkusumo National Referral Hospital. Amino acids examination was using dried blood spots (DBSs) sample and Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) method. We examined 10 non-essential (alanine, arginine, aspartic acid, glutamic acid, asparagine, glycine, glutamine, proline, serine, tyrosine), 9 essentials (histidine, phenylalanine, isoleucine, leucine, lysine, methionine, threonine, tryptophan, valine), and 2 special (ornithine, citrulline) amino acids. The results showed that almost all amino acids were lower (6 non-essential, 8 essentials, and citrulline), whereas others were higher (aspartate acid, serine) or normal (glutamic acid, glycine, methionine, and ornithine) than normal reference value from Mayo. CKD patients on hemodialysis have decreased amino acid especially essential amino acids. These results can be used in modification of amino acid supplementation CKD patient on hemodialysis in Indonesia. © 2021 Author(s). |
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American Institute of Physics Inc. |
0094243X |
9780735440968 |
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Conference Paper |
- |
177 |
20880 |
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434 |
Pustimbara A., Putri D.C., Prakoso N.M., Priambodo R., Ariani Y., Yuliarti K., Bowolaksono A., Sjarif D.R. |
57217086984;57204606877;57214084050;57190937999;57200504713;54917483500;57205093224;6506242684; |
Novel base alterations at intron 3 of 6-pyruvoyl-tetrahydropterin synthase gene in Indonesian population |
2021 |
AIP Conference Proceedings |
2331 |
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050028 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103860378&doi=10.1063%2f5.0042047&partnerID=40&md5=80d1cbd9c9334c733fed5caccbb5bdad |
Human Genetics Research Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, 10430, Indonesia; Department of Pediatric, Universitas Indonesia, RSUPN, Dr. Cipto Mangunkusumo, Jakarta, 10430, Indonesia; Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia |
Pustimbara, A., Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia; Putri, D.C., Human Genetics Research Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, 10430, Indonesia, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia; Prakoso, N.M., Human Genetics Research Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, 10430, Indonesia; Priambodo, R., Human Genetics Research Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, 10430, Indonesia; Ariani, Y., Human Genetics Research Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, 10430, Indonesia, Department of Pediatric, Universitas Indonesia, RSUPN, Dr. Cipto Mangunkusumo, Jakarta, 10430, Indonesia, Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Depok, Indonesia; Yuliarti, K., Human Genetics Research Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, 10430, Indonesia, Department of Pediatric, Universitas Indonesia, RSUPN, Dr. Cipto Mangunkusumo, Jakarta, 10430, Indonesia; Bowolaksono, A., Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia; Sjarif, D.R., Human Genetics Research Center, Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jakarta, 10430, Indonesia, Department of Pediatric, Universitas Indonesia, RSUPN, Dr. Cipto Mangunkusumo, Jakarta, 10430, Indonesia |
6-pyruvoyl-tetrahydropterin synthase (PTPS) or tetrahydrobiopterin (BH4) deficiency is the most common enzyme synthesis defect which was reported to cause of hyperphenylalaninemia. This deficiency is caused by pathogenic variant in exons and introns of 6-pyruvoyl-tetrahydropterin synthase (PTS) gene in chromosome 11q22.3-q23.3. This study is focused on the detection of DNA variants in intron 3 especially for insertion and base alteration. Methods that has been carried out in this study are DNA isolation, polymerase chain reaction (PCR), the visualization of PCR products through DNA electrophoresis, and Sanger sequencing. A total 29 variants have been characterized in this study, obtained from the DNA of one Indonesian PTPS patients and 33 healthy individuals as control. Those alterations were categorized into substitution and intronic insertion and located in the sequence of intron 3 and 4 of PTS gene. Further analyses are required to be performed to characterize the effect of identified variants to the splicing events of PTS mRNA. © 2021 Author(s). |
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American Institute of Physics Inc. |
0094243X |
9780735440753 |
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Conference Paper |
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177 |
20880 |
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