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860 |
Wuyung P.E., Rahadiati F.B., Tjahjadi H., Salinah S., Kusmardi K., Kodariah R., Wiweko B. |
57192889605;57222312428;57210953454;57222311659;56966625300;14010667100;43061741400; |
Histopathology and Arid1a Expression in Endometriosis-Associated Ovarian Carcinoma (EAOC) Carcinogenesis Model with Endometrial Autoimplantation and DMBA Induction |
2021 |
Asian Pacific Journal of Cancer Prevention |
22 |
2 |
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553 |
558 |
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3 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102225233&doi=10.31557%2fAPJCP.2021.22.2.553&partnerID=40&md5=0851ed3cc33bf297db42accf77d7988a |
Department of Anatomical Pathology, Medicine Universitas Indonesia; Animal Research Facilities, Indonesian Medical Education and Research Institute, Medicine Universitas Indonesia; Specialty Programme in Anatomical Pathology, Department of Anatomical Pathology, Medicine Universitas Indonesia; Department of Obstetrics and Gynecology, Medicine Universitas Indonesia; Indonesian Medical Education and Research Institute, Medicine Universitas Indonesia |
Wuyung, P.E., Department of Anatomical Pathology, Medicine Universitas Indonesia, Animal Research Facilities, Indonesian Medical Education and Research Institute, Medicine Universitas Indonesia; Rahadiati, F.B., Specialty Programme in Anatomical Pathology, Department of Anatomical Pathology, Medicine Universitas Indonesia; Tjahjadi, H., Department of Anatomical Pathology, Medicine Universitas Indonesia; Salinah, S., Department of Anatomical Pathology, Medicine Universitas Indonesia; Kusmardi, K., Department of Anatomical Pathology, Medicine Universitas Indonesia; Kodariah, R., Department of Anatomical Pathology, Medicine Universitas Indonesia; Wiweko, B., Department of Obstetrics and Gynecology, Medicine Universitas Indonesia, Indonesian Medical Education and Research Institute, Medicine Universitas Indonesia |
Background: Ovarian carcinoma is one of the most deadly malignancies in the gynecologic field. The cause is not yet known, and the clinical symptoms are not specific. Endometrioid carcinoma and ovarian clear cell carcinoma can originate from endometriosis and are known as endometriosis-related ovarian carcinoma (EAOC). Development of EAOC experimental animal models is needed for basic research and clinical preparation of human tissue tests. This study aimed to determine the role of the Arid1a gene mutation in the carcinogenetic process of EAOC in experimental animal models induced with DMBA. Methods: In this study, the EAOC experimental model was developed using the autoimplantation technique and DMBA induction. This study involved placebo surgery mice (sham), endometrial autoimplantation, and a combination of endometrial autoimplantation and DMBA induction, which were sacrificed at weeks 5, 10, and 20, respectively. Histopathological assessment and immunohistochemical Arid1a staining with an assessment of positive percentages were carried out on 200 cells. Results: This study produced 1 (20%) atypical endometriosis and 1 (20%) clear cell carcinoma at implantation and after 10 weeks of DMBA induction, and 100% endometrioid carcinoma in the DMBA-induced group. Arid1a staining did not show any significant difference (p = 0.313) in all groups. Conclusion: The combination of endometrial autoimplantation techniques and DMBA induction in the ovary produced atypical endometriosis, clear cell carcinoma, and endometrioid carcinoma, where time is an important factor. There was no significant difference in Arid1a expression between the treatment and control groups. © 2021. All Rights Reserved. |
Arid1a; DMBA; EAOC; Endometriosis; experimental animal model |
ARID1A protein, human; DNA binding protein; transcription factor; animal; autotransplantation; carcinoma; complication; disease model; endometriosis; endometrium; female; metabolism; ovary tumor; pathology; rat; transplantation; Animals; Carcinoma; Disease Models, Animal; DNA-Binding Proteins; Endometriosis; Endometrium; Female; Ovarian Neoplasms; Rats; Transcription Factors; Transplantation, Autologous |
Asian Pacific Organization for Cancer Prevention |
15137368 |
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33639673 |
Article |
Q2 |
512 |
9866 |
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861 |
Umas R.A., Supriadi S., Whulanza Y., Hasan A.A.A.F., Kreshanti P. |
57222252781;16040272500;36807053500;57222257599;36192866200; |
Evaluating the Gear Stress of Novel Reverse Rotation Bit Manual Screwdriver Design for Miniscrew Implants |
2021 |
Journal of Mechanical Engineering |
18 |
1 |
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175 |
192 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102086175&partnerID=40&md5=0196752c2bad6ab9b05bb97c07712c5b |
Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, 16424, Indonesia; Cleft and Craniofacial Center Cipto Mangunkusumo Hospital, Plastic and Reconstructive Surgery Division, Department of Surgery, Faculty of Medicine, Universitas Indonesia, Indonesia |
Umas, R.A., Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, 16424, Indonesia; Supriadi, S., Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, 16424, Indonesia; Whulanza, Y., Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, 16424, Indonesia; Hasan, A.A.A.F., Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, 16424, Indonesia; Kreshanti, P., Cleft and Craniofacial Center Cipto Mangunkusumo Hospital, Plastic and Reconstructive Surgery Division, Department of Surgery, Faculty of Medicine, Universitas Indonesia, Indonesia |
One of the problems faced by the screwdrivers used for craniomaxillofacial implant fixation is that the maximum torque required for implant removal is significantly higher than the maximum torque needed for inserting implants. Another problem is that the hand torque produced by a right-handed person is lower in the counterclockwise direction, which is the removal direction for miniscrews. The novel design presented here of a manual screwdriver equipped with an epicyclic gear will produce a reverse bit rotation and provide the mechanical advantage of higher torque output. In this study, simulations were conducted by varying the torque input within the range of 0-1000 Nmm with an applied load in each simulation to be adapted based on the epicyclic gear set. The materials used in this study are AISI 316L and Ti6Al4V. The maximum Von Mises stress value was observed in the sun gear from the second gear set at 522.59 MPa (AISI 316L) and 430.76 MPa (Ti6Al4V) for the maximum torque input, which was followed by the planetary and ring gear from the second gear set and then the planetary gear, ring gear, and sun gear from the first gear set. The total deformation also showed the difference between the two materials; the deformation when using AISI 316L as the material was lower than when using Ti6Al4V. © 2021 Faculty of Mechanical Engineering. All Rights Reserved. |
Epicyclic Gear; Maxillofacial Implant; Surgical Screwdriver |
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UiTM Press |
18235514 |
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Article |
Q3 |
216 |
18402 |
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862 |
Putri M.I.A., Panigoro S.S., Harahap A.S., Pakasi T.A., Brahma B. |
57222261965;56790104300;57218511857;23980778600;55675752700; |
Acetic Acid and Iodine Staining for Determining Malignancy in Solid Tumors |
2021 |
Asian Pacific Journal of Cancer Prevention |
22 |
2 |
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463 |
469 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102041256&doi=10.31557%2fAPJCP.2021.22.2.463&partnerID=40&md5=a2eb3a6ba0cc657be963217a4e5d6cce |
Surgical Oncology Division, Department of Surgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Surgical Oncology, Dharmais Cancer Hospital, National Cancer Center, Jakarta, Indonesia |
Putri, M.I.A., Surgical Oncology Division, Department of Surgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Panigoro, S.S., Surgical Oncology Division, Department of Surgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Harahap, A.S., Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Pakasi, T.A., Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Brahma, B., Department of Surgical Oncology, Dharmais Cancer Hospital, National Cancer Center, Jakarta, Indonesia |
Objective: Surgical margin is an important prognostic factor in solid cancer surgery. Frozen section (FS), the gold standard for intraoperative surgical margin evaluation, requires extensive waiting time and expensive FS devices. The purpose of this diagnostic study was to verify whether multi-staining (MS) method with acetic acid and iodine could be used to differentiate malignant and non-malignant lesions of solid tumor. Methods: The study was conducted on patients with solid tumor who underwent surgery in the Surgical Oncology Division of Dr. Cipto Mangunkusumo General Hospital from December 2017 to April 2018. Samples measuring less than 5 mm, necrotic tissue sample, and patients who did not agree to participate in the study were excluded. Every specimen was divided into two, one side as unstained control and the other side as MS samples. MS samples were sprayed with 10% acetic acid combined with iodine. MS samples and unstained controls were sent for histopathologic results and the pathologist was blinded to group assignment. Acetowhitening reaction in the sample was an indication of a positive MS result, and the presence of malignant foci in the histopathology examination was classified as positive pathological results. Results: Five-hundredand-twenty samples were obtained from 150 patients. MS method was found to have sensitivity and specificity of 82%, and 63.5%, respectively. In subgroup analysis, we found that MS method has a sensitivity and specificity of 100% and 79.3%, respectively for epithelial breast tumor; 65.7% and 83.3%, respectively for thyroid nodules; and 94.1% and 33.3%, respectively for oral cavity tumors. MS method reacts positively to solid malignant tumor and negatively to benign tumor and normal tissue (from margin samples). Highest sensitivity was found for breast and oral cavity malignancy, and high specificity was found for thyroid cancers. Conclusion: This study provided an alternative staining method for intraoperative macroscopic surgical margin evaluation, especially for rural areas without frozen section facilities. © This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License. |
Acetic acid; breast cancer; frozen section; iodine; oral cancer; solid tumor; thyroid cancer; vital staining |
acetic acid; coloring agent; dyes, reagents, indicators, markers and buffers; iodide; Lugol's solution; adolescent; adult; aged; child; cross-sectional study; female; frozen section; human; male; middle aged; neoplasm; pathology; sensitivity and specificity; staining; surgical margin; young adult; Acetic Acid; Adolescent; Adult; Aged; Child; Coloring Agents; Cross-Sectional Studies; Female; Frozen Sections; Humans; Indicators and Reagents; Iodides; Male; Margins of Excision; Middle Aged; Neoplasms; Sensitivity and Specificity; Staining and Labeling; Young Adult |
Asian Pacific Organization for Cancer Prevention |
15137368 |
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33639661 |
Article |
Q2 |
512 |
9866 |
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864 |
Indriyani, Liem I.K., Wuyung P.E., Adnindya M.R., Nasution A.A., Wardiansah, Jusuf A.A. |
57195988823;55802927800;57192889605;57195990858;57195984460;57222188547;57192275719; |
The dynamic of afamin and alpha-fetoprotein expression during rat liver development |
2021 |
OnLine Journal of Biological Sciences |
21 |
1 |
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26 |
32 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101833482&doi=10.3844%2fojbsci.2021.26.32&partnerID=40&md5=63694adea4e7ba46c95652e49c319ed0 |
Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Anatomy, Faculty of Medicine, Universitas Muhammadiyah Palembang, Palembang, Indonesia; Integrated Laboratory, Faculty of Medicine, Universitas Indonesia, Indonesia; Departement of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Anatomy, Faculty of Medicine, Sriwijaya University, Palembang, Indonesia; Department of Anatomy, Faculty of Medicine and Health Science, Universitas Bengkulu, Bengkulu, Indonesia; Departement of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Indriyani, Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Department of Anatomy, Faculty of Medicine, Universitas Muhammadiyah Palembang, Palembang, Indonesia; Liem, I.K., Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Integrated Laboratory, Faculty of Medicine, Universitas Indonesia, Indonesia; Wuyung, P.E., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Departement of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Adnindya, M.R., Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Department of Anatomy, Faculty of Medicine, Sriwijaya University, Palembang, Indonesia; Nasution, A.A., Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Department of Anatomy, Faculty of Medicine and Health Science, Universitas Bengkulu, Bengkulu, Indonesia; Wardiansah, Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Department of Anatomy, Faculty of Medicine, Sriwijaya University, Palembang, Indonesia; Jusuf, A.A., Departement of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Liver has a high level of Alpha-fetoprotein which is assumed to be important for mammalian development. However, Alpha-fetoprotein knockout studies in mice suggested that despite the absence of Alpha-fetoprotein, mice developed normally; the only abnormality observed was infertility in female mice. There are indications that Afamin, a protein that has a gene sequence located on the same chromosome as Alpha-fetoprotein, may compensate for the absence of Alpha-fetoprotein during embryonic development. Nevertheless, research on the dynamics of Afamin expression and its correlation with Alpha-fetoprotein has not been reported. Therefore, it has been done a baseline study to determine the pattern and distribution of Alpha-fetoprotein expression and its correlation with Afamin expression in the developing rat liver. An analytic observational study was performed to study the expression of Afamin and Alpha-fetoprotein in the rat embryos (embryonic day/ED12.5, ED14.5, ED16.5, ED18.5), neonates and adults using an immunohistochemistry technique by assessing the location and intensity of expression using the Immunohistochemistry Optical density score. Afamin started to express in ED18.5 and was evenly distributed in the hepatocytes and was maintained until adulthood. Whereas, Alpha-fetoprotein has been seen at ED12.5 and was distributed evenly in the hepatoblast. At ED18.5, Alpha-fetoprotein expression reached a peak and decreased dramatically after birth. Spearman correlation test showed that both proteins' expressions were correlated in the opposite direction (P<0.05 and r =-0.695). In conclusion, Afamin and Alpha-fetoprotein have an opposite expression during development. The time point of intersection was ED18.5; implying the peak of hepatoblast proliferation to enter the differentiation process. © 2021 Indriyani, Isabella Kurnia Liem, Puspita Eka Wuyung, Msy Rulan Adnindya, Ahmad Azmi Nasution, Wardiansah and Ahmad Aulia Jusuf. |
Afamin; Alpha-Fetoprotein; Hepatoblast; Hepatocytes; Liver Development |
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Science Publications |
16084217 |
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Article |
Q4 |
152 |
23000 |
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865 |
Silitonga P., Dewi B.E., Bustamam A., Al-Ash H.S. |
57219406661;24076058600;36815737800;57205062769; |
Evaluation of Dengue Model Performances Developed Using Artificial Neural Network and Random Forest Classifiers |
2021 |
Procedia Computer Science |
179 |
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135 |
143 |
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1 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101780236&doi=10.1016%2fj.procs.2020.12.018&partnerID=40&md5=bf5f6b6b59db9e4a155ff56ac22ceeaf |
Department of Mathematics, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Kampus Baru UI, Depok, 16424, Indonesia; Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.5, Jakarta Pusat, Jakarta, 10430, Indonesia; Department of Computer Science, Faculty of Computer Science, Universitas Indonesia, Kampus Baru UI, Depok, 16424, Indonesia |
Silitonga, P., Department of Mathematics, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Kampus Baru UI, Depok, 16424, Indonesia; Dewi, B.E., Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.5, Jakarta Pusat, Jakarta, 10430, Indonesia; Bustamam, A., Department of Mathematics, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Kampus Baru UI, Depok, 16424, Indonesia; Al-Ash, H.S., Department of Computer Science, Faculty of Computer Science, Universitas Indonesia, Kampus Baru UI, Depok, 16424, Indonesia |
Dengue is one of the endemic diseases in Indonesia. Dengue is being suffered by many people, regardless of their gender and age. Therefore, research about dengue based on dengue patients' data was conducted. There was a lot of information written in that data regarding the corresponding patients and the dengue they had suffered, such as gender, age, how long the patients were hospitalized, the symptoms they experienced, and laboratory characteristics results. Diagnosis of each of the corresponding patients based on their symptoms and laboratory characteristics results were also written in that data. The diagnoses were classified into three different clinical degrees according to the severity level, which is DF as the mild level, DHF grade 1 as the intermediate level, and DHF grade 2 as the severe level. In this research, data of the patients on the third day of being hospitalized was analyzed, because, on the third day, dengue is entering a critical phase. The objectives of this research were: to evaluate the performance of the models that were used to predict the correct class within the given dataset developed using Artificial Neural Network (ANN) classifier and Random Forest (RF) classifier separately, and to find a classifier that yielded the best performance. The results obtained from this research will be used in the development of a Machine Learning model that can predict the clinical degree of dengue in the critical phase, if the laboratory characteristics results are known, using a classifier that yielded the best performance. © 2021 Elsevier B.V.. All rights reserved. |
Artificial Neural Network; Dengue; Random Forest |
Classification (of information); Clinical research; Decision trees; Diagnosis; Intelligent computing; Random forests; Turing machines; Indonesia; Intermediate level; Machine learning models; Model performance; Random forest classifier; Neural networks |
Elsevier B.V. |
18770509 |
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Conference Paper |
- |
334 |
13810 |
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866 |
Lesmana C.R.A., Herjuningtyas C., Inggriani S., Pratiwi Y.E., Lesmana L.A. |
8977683000;57222165389;35329838800;57222163306;55920139300; |
Bedside Percutaneous Approach in a Critically Ill ICU Patient with Complex Pancreatobiliary Disorder Followed by Endoscopic Approach: Lessons Learnt from a Tertiary Referral Center |
2021 |
Case Reports in Gastroenterology |
15 |
1 |
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210 |
217 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101734257&doi=10.1159%2f000513282&partnerID=40&md5=b06514b9ba8afa5ed7ce24173ab5b336 |
Hepatobiliary Division, Department of Internal Medicine, Dr. Cipto Mangunkusumo National General Hospital, Medical Faculty University of Indonesia, Jakarta, Indonesia; Digestive Disease and GI Oncology Center, Medistra Hospital, Jakarta, Indonesia; Department of Anesthesiology, Medistra Hospital, Jakarta, Indonesia; Department of Radiology, Medistra Hospital, Jakarta, Indonesia |
Lesmana, C.R.A., Hepatobiliary Division, Department of Internal Medicine, Dr. Cipto Mangunkusumo National General Hospital, Medical Faculty University of Indonesia, Jakarta, Indonesia, Digestive Disease and GI Oncology Center, Medistra Hospital, Jakarta, Indonesia; Herjuningtyas, C., Department of Anesthesiology, Medistra Hospital, Jakarta, Indonesia; Inggriani, S., Department of Radiology, Medistra Hospital, Jakarta, Indonesia; Pratiwi, Y.E., Digestive Disease and GI Oncology Center, Medistra Hospital, Jakarta, Indonesia; Lesmana, L.A., Digestive Disease and GI Oncology Center, Medistra Hospital, Jakarta, Indonesia |
Pancreatobiliary disorder is a challenging clinical condition, especially when this condition is causing severe infection or biliary sepsis, and sometimes it requires intensive care unit (ICU) treatment. Biliary drainage is the mainstay of therapy; however, the choice of the drainage method is dependent on the patient's clinical condition and the disease itself. A 79-year-old female was transferred on a ventilator to our ICU from another hospital due to biliary sepsis, a large common bile duct stone, and an infected pancreatic pseudocyst. The patient also has other comorbidities such as heart problems, hypothyroidism, and diabetes mellitus. Bedside percutaneous transhepatic biliary drainage without fluoroscopy and percutaneous cyst aspiration was successfully performed, which improved the patient's condition; this was followed by an endoscopic approach, i.e., endoscopic retrograde cholangiopancreatography (ERCP) and endoscopic ultrasound-guided pancreatic pseudocyst drainage. The clinical improvement showed itself in the change of the patient's respiratory status and ventilator mode. In conclusion, the percutaneous approach has a big role in managing critically ill patients in the ICU setting. However, expertise, training experience, and a multidisciplinary team approach are very important for successful management and patient outcome. © 2021 The Author(s). Published by S. Karger AG, Basel. |
Biliary sepsis; Common bile duct stone; Pancreatic pseudocyst; Percutaneous approach |
amikacin; antibiotic agent; bilirubin; C reactive protein; carcinoembryonic antigen; insulin; levothyroxine; piperacillin plus tazobactam; triacylglycerol lipase; aged; Article; artificial ventilation; biliary tract drainage; biliary tract endoscopy; case report; clinical article; common bile duct stone; comorbidity; continuous positive airway pressure; critically ill patient; cyst aspiration; diabetes mellitus; echography; endoscopic retrograde cholangiopancreatography; female; fluoroscopy; human; hypothyroidism; intensive care unit; jaundice; magnetic resonance cholangiopancreatography; pancreas disease; pancreas pseudocyst; sepsis |
S. Karger AG |
16620631 |
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Article |
Q3 |
247 |
16829 |
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867 |
Ibrahim F., Sahlan M., Ginting M.J., Kartika Pratami D., Hermansyah H., Wijanarko A. |
54886001500;57189182661;57212472114;57222140683;15759436800;6506885367; |
Isolation of protein from the spine venom of pterois volitans found in the indonesian ocean, using a heating process, for anticancer, antiretroviral, antibacterial, and antioxidant assays |
2021 |
International Journal of Applied Pharmaceutics |
13 |
special issue 2 |
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53 |
58 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101657433&doi=10.22159%2fijap.2021.v13s2.10&partnerID=40&md5=7efd709314f7d32aab3f98fec52eeb8d |
Virology and Cancer Pathobiology Research Centre, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, 10320, Indonesia; Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, Indonesia; Research Centre for Biomedical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, Indonesia; Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok, West Java, 16424, Indonesia; Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Pancasila University, South Jakarta, DKI Jakarta, 12640, Indonesia |
Ibrahim, F., Virology and Cancer Pathobiology Research Centre, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, 10320, Indonesia; Sahlan, M., Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, Indonesia, Research Centre for Biomedical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, Indonesia; Ginting, M.J., Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok, West Java, 16424, Indonesia; Kartika Pratami, D., Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Pancasila University, South Jakarta, DKI Jakarta, 12640, Indonesia; Hermansyah, H., Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, Indonesia; Wijanarko, A., Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, Indonesia |
Objective: This research investigates the antibacterial, anticancer, antioxidant, and antiretroviral activities of the lionfish spine poison extract. Methods: Isolation and purification of the phospholipase A2 (PLA2) protein obtained from the spine poison were conducted through the following stages, including, extraction of the venom by sonication, heating, and purification using gradual saturation levels of ammonium sulfate. Furthermore, the purity and concentration of PLA2 were analyzed using the Lowry test and Marinetti’s method, respectively, while its protein content was ascertained through SDS-PAGE. Toxicity was then evaluated employing the brine shrimp lethality test (BSLT), and its anticancer activity was assessed in human cervical carcinoma cells (HeLa cells). Finally, its antioxidant, antibacterial, and antiretroviral activities were analyzed using the DPPH method, agar diffusion test against Salmonella sp. and E. coli, and SRV-2 and RT-qPCR tests, respectively. Results: The protein demonstrated 37.79% inhibition for anticancer activity, IC50 1312 ppm for antioxidant activity, 98.81%, and 89.28% inhibition of E. coli and Salmonella sp. respectively for antibacterial activity and 98.13% inhibition for antiretroviral activity. Conclusion: It can be concluded that lionfish (Pterois volitans) has the potential to be developed as an antioxidant, anticancer, antibacterial, and antiretroviral agent. Furthermore, the pharmacological activity of its spine venom was determined by isolating PLA2 protein from its extract, using an optimum heating temperature of 70 °C and an ammonium sulfate saturation level of 80%. © 2021 The Authors. Published by Innovare Academic Sciences Pvt Ltd. T. |
Antibacterial; Anticancer; Antioxidant; Antiretroviral; Crude venom; Pterois volitans |
ammonium sulfate; antineoplastic agent; antioxidant; antiretrovirus agent; caspase 3; chloramphenicol; phospholipase A2; venom; agar diffusion; antibacterial activity; antineoplastic activity; antioxidant activity; antiviral activity; Article; bacterial growth; controlled study; cytotoxicity; DNA extraction; DPPH radical scavenging assay; enzyme activity; enzyme inhibition; enzyme linked immunosorbent assay; Escherichia coli; heating; HeLa cell line; human; human cell; hydroxyl radical scavenging assay; IC50; minimum inhibitory concentration; MTT assay; nonhuman; oxygen saturation; polyacrylamide gel electrophoresis; protein content; protein isolation; protein purification; Pterois volitans; Salmonella; shrimp; Staphylococcus aureus; temperature; ultrasound; ultraviolet visible spectroscop |
Innovare Academics Sciences Pvt. Ltd |
09757058 |
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Article |
Q3 |
238 |
17272 |
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868 |
Adhimulia K.J., Alfariq L., Kamal A.F. |
57222107905;57222109165;56648996700; |
Atypical telangiectatic osteosarcoma |
2021 |
Formosan Journal of Surgery |
54 |
1 |
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28 |
31 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101461287&doi=10.4103%2ffjs.fjs_63_20&partnerID=40&md5=455cb3287eb4bb8748ca09335f8c6946 |
Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Department of Orthopedic and Traumatology, Faculty of Medicine, Cipto Mangunkusumo General Hospital, University of Indonesia, Jakarta, Indonesia; Division of Orthopedic Oncology, Department of Orthopedic and Traumatology, Faculty of Medicine, Cipto Mangunkusumo General Hospital, University of Indonesia, Jakarta, Indonesia |
Adhimulia, K.J., Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Alfariq, L., Department of Orthopedic and Traumatology, Faculty of Medicine, Cipto Mangunkusumo General Hospital, University of Indonesia, Jakarta, Indonesia; Kamal, A.F., Division of Orthopedic Oncology, Department of Orthopedic and Traumatology, Faculty of Medicine, Cipto Mangunkusumo General Hospital, University of Indonesia, Jakarta, Indonesia |
Telangiectatic osteosarcoma (TOS) is a rare malignant bone tumor. The main clinical and radiological significance of the case presentation is TOS can be easily misdiagnosed as a benign lesion such as an aneurysmal bone cyst (ABC). In this case, we present 63-year-old male with a large mass in the left leg. Imaging modalities were obtained in this patient to define the diagnosis. In this atypical case of TOS, relatively old age presentation, slow-progressing lesion, well-demarcated lesion, and lack of periosteal reaction further accentuate the similarity between TOS and ABC or other benign lesion causing high rate misdiagnosis of TOS. The histopathological examination will finally differentiate TOS from ABC. © 2021 Wolters Kluwer Medknow Publications. All rights reserved. |
Aneurysmal bone cyst; atypical presentation; telangiectatic osteosarcoma |
bone cement; lactate dehydrogenase; adult; aneurysmal bone cyst; arthrodesis; Article; atypical telangiectatic osteosarcoma; bone radiography; case report; clinical article; clinical assessment; clinical feature; comparative study; computer assisted tomography; diagnostic accuracy; diagnostic error; diaphysis; disease exacerbation; epiphysis; erythrocyte sedimentation rate; histopathology; hospital admission; human; knee; laboratory test; male; medical history; metaphysis; middle aged; mitosis; multimodal imaging; neoadjuvant chemotherapy; nuclear magnetic resonance imaging; osteosarcoma; patient referral; periosteum; physical examination; priority journal; proximal tibia; skin edema; skin induration; soft tissue; stroma cell; telangiectasia; thorax radiography; tibialis anterior muscle; t |
Wolters Kluwer Medknow Publications |
1682606X |
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Article |
Q4 |
135 |
24781 |
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869 |
Fabiani H., Mudjihartini N., Lestari W. |
57222097497;57191055759;57222081857; |
Dietary Omega-6 to Omega-3 Fatty Acids Ratio is Correlated with High Molecular Weight Adiponectin Level in Indonesian Office Workers |
2021 |
International Journal of Nutrition, Pharmacology, Neurological Diseases |
11 |
1 |
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70 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101380278&doi=10.4103%2fijnpnd.ijnpnd-89-20&partnerID=40&md5=7be1b049e89a83256f279f76e4645583 |
Department of Nutrition, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Department of Nutrition, Faculty of Medicine and Health Sciences, Krida Wacana Christian University, Jakarta, Indonesia; 3Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia |
Fabiani, H., Department of Nutrition, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia, Department of Nutrition, Faculty of Medicine and Health Sciences, Krida Wacana Christian University, Jakarta, Indonesia; Mudjihartini, N., 3Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia; Lestari, W., Department of Nutrition, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia |
Context: Adiponectin is an adipokine that is secreted by adipocytes and acts to prevent obesity and obesity-related disorders. The high ratio of omega-6 to omega-3 fatty acids in modern dietary habits in working-Age populations, urban areas in particular, is known to play a role in adiponectin secretion. However, previous studies showed inconclusive results. Aim: The study aims to determine the association of the dietary omega-6 to omega-3 fatty acids ratio with adiponectin levels in office workers in Jakarta, Indonesia. Settings and Design: This cross-sectional study included 55 office workers in East Jakarta, Indonesia. Methods: Data were collected via questionnaire, 3-day food record, anthropometric measurement, and serum collection. Statistical analysis used: Independent t test was applied to assess the relationship between subjects characteristics and dietary intake with high molecular weight adiponectin levels. Association of dietary omega-6/omega-3 fatty acids ratio and adiponectin were evaluated using Pearson correlation test (P < 0.05). Results: There is no statistically significant difference in adiponectin levels based on waist circumference category, as well as categories of physical activity, household income levels, total energy, and total fat intake. Conversely, in female subjects (P = 0.000), subjects with normal body mass index (P = 0.000), higher education levels (P = 0.031), and nonsmoking subjects (P = 0.019), significantly higher adiponectin levels are obtained. The ratio of dietary omega-6/omega-3 fatty acids is negatively correlated with adiponectin (P = 0.004, r =-0.383). Conclusions: The decrease in the ratio of omega-6/omega-3 fatty acids is inversely related to higher level of adiponectin, indicating that dietary changes may potentially play a role in prevention strategies for obesity and obesity-related diseases. © 2021 Wolters Kluwer Medknow Publications. All rights reserved. |
HMW adiponectin; obesity; omega-3 fatty acid; omega-6 fatty acid; workers |
adiponectin; omega 3 fatty acid; omega 6 fatty acid; adult; Article; body mass; caloric intake; cross-sectional study; cytokine release; data analysis software; dietary intake; educational status; fat intake; female; food frequency questionnaire; household income; human; Indonesia; male; metabolic equivalent; molecular weight; non-smoker; obesity; office worker; physical activity; protein expression; sex difference; waist circumference |
Wolters Kluwer Medknow Publications |
22310738 |
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Article |
Q4 |
177 |
20903 |
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870 |
Prasetyo M., Rahardja R.R., Yanuar A., Prihartono J., Setiawan S.I. |
57192905252;57222100194;57222087272;6602605635;57195939543; |
Ultrasonography evaluation of the normal ulnar nerve in adult: Comparison of the cross-sectional area at elbow extension and flexion |
2021 |
European Journal of Radiology Open |
8 |
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100331 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101370509&doi=10.1016%2fj.ejro.2021.100331&partnerID=40&md5=f9e415e711bd67bbe8b7b71cf1b20376 |
Department of Radiology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Central General Hospital, Jakarta, Indonesia; Department of Neurology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Central General Hospital, Jakarta, Indonesia; Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Prasetyo, M., Department of Radiology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Central General Hospital, Jakarta, Indonesia; Rahardja, R.R., Department of Radiology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Central General Hospital, Jakarta, Indonesia; Yanuar, A., Department of Neurology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Central General Hospital, Jakarta, Indonesia; Prihartono, J., Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Setiawan, S.I., Department of Radiology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo National Central General Hospital, Jakarta, Indonesia |
Background: Cross-sectional area (CSA) measurement of the ulnar nerve in the adult population by using ultrasonography (US) at elbow extension and flexion has previously been reported, but not much evidence showed a significant difference between elbow extension and flexion position. Purpose: To compare the ulnar nerve CSA between elbow extension and flexion position. Methods: A comparative cross-sectional study was conducted by involving 36 healthy adults with normally functioning ulnar nerve proven by Nerve Conduction Study (NCS) or Electroneurography. The ulnar nerve CSA was measured on each elbow by using US at the level of the medial epicondyle, 2 cm distal and 2 cm proximal from the medial epicondyle. Results: The average ulnar nerve CSA at the medial epicondyle, 2 cm distal and proximal to the medial epicondyle at elbow extension respectively were 5.95 ± 0.74 mm2, 6.27 ± 0.92 mm2, and 5.92 ± 0.73 mm2. At elbow flexion, the average ulnar nerve CSA at the positions was 5.70 ± 0.83 mm2, 5.23 ± 0.87 mm2, dan 5.73 ± 0.71 mm2 respectively. The CSA of the ulnar nerve at elbow extension was significantly larger compared to the flexion position in the three areas observed in this study (p < 0.001). Conclusion: The CSA of the ulnar nerve at elbow extension position was larger compared to the flexion position. Elbow position should be considered in measuring CSA of the ulnar nerve. © 2021 The Author(s) |
Cross-sectional area; Medial epicondyle; Ulnar nerve; Ultrasonography |
adult; article; clinical article; controlled study; cross-sectional study; echography; elbow flexion; electroneurography; female; human; human experiment; male; nerve conduction; ulnar nerve |
Elsevier Ltd |
23520477 |
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Article |
Q3 |
490 |
10260 |
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