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348 |
Djuardi Y., Lazarus G., Stefanie D., Fahmida U., Ariawan I., Supali T. |
6507800820;57214599425;57193121018;14420783500;8840573400;6602742029; |
Soil-transmitted helminth infection, anemia, and malnutrition among preschool-age children in nangapanda subdistrict, indonesia |
2021 |
PLoS Neglected Tropical Diseases |
15 |
6 |
e0009506 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85110433985&doi=10.1371%2fjournal.pntd.0009506&partnerID=40&md5=acf6b372e3919f6422f75ae7d13a1c91 |
Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Southeast Asian Ministers of Education Organization Regional Centre for Food and Nutrition (SEAMEO RECFON), Pusat Kajian Gizi Regional Universitas Indonesia, Jakarta, Indonesia; Department of Biostatistics and Population Studies, Faculty of Public Health, Universitas Indonesia, Depok, Indonesia |
Djuardi, Y., Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Lazarus, G., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Stefanie, D., Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Fahmida, U., Southeast Asian Ministers of Education Organization Regional Centre for Food and Nutrition (SEAMEO RECFON), Pusat Kajian Gizi Regional Universitas Indonesia, Jakarta, Indonesia; Ariawan, I., Department of Biostatistics and Population Studies, Faculty of Public Health, Universitas Indonesia, Depok, Indonesia; Supali, T., Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Background Soil-transmitted helminth (STH) infections are still prevalent in Indonesia, with roughly one-third of infected population being preschool-age children (PSC), which are generally at higher risk of morbidity such as malnutrition and anemia. This study aimed to investigate the association of STH infections with nutritional status and anemia among PSC in Nangapanda subdistrict, Ende, East Nusa Tenggara. Methods A cross-sectional survey involving PSC ranging from 12 to 59 months old from Nangapanda subdistrict, Ende district, East Nusa Tenggara was performed. Socio-demographic, breastfeeding, and complementary feeding information was obtained from structured questionnaires, while nutritional and anemia status was determined from anthropometry and hemoglobin measurements, respectively. Anthropometric z-scores were calculated based on the World Health Organization 2006 standards and stool samples were examined using Kato-Katz method. Results A total of 393 PSC randomly selected from 22 villages were examined. The prevalence of underweight, stunting, wasting, and anemia were 33.1%, 40.2%, 17.1%, and 60.3%, respectively. STH infection, predominated by Ascaris lumbricoides, was found in 160 (58.8%) PSC. Single STH infection, but not multiple infection, was independently associated with a lower risk of anemia (odds ratio [OR] 0.320, 95% confidence interval [CI]: 0.126– 0.809, p = 0.016). Similar association with anemia was also found on mild STH infection (OR 0.318 [95% CI: 0.114–0.887], p = 0.029). On the other hand, younger children were found to have a higher risk of anemia and stunting. None of the examined variables were independently associated with underweight and wasting. Conclusion STH infection as well as anemia and malnutrition were prevalent in this region. However in this study, current STH infections seemed to have minimal negative impact on children’s nutritional status. © 2021 Djuardi et al. |
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edetic acid; hemoglobin; hemoglobin; anemia; anthropometry; antiretroviral therapy; Article; body mass; breast feeding; child; complementary feeding; controlled study; cross-sectional study; disorder of sex development; endoscopic retrograde cholangiopancreatography; feces analysis; female; flotation; food frequency questionnaire; food intake; helminthiasis; human; major clinical study; male; malnutrition; mass spectrometry; morbidity; nutritional status; obesity; preschool child; questionnaire; randomized controlled trial; risk factor; Strongyloides stercoralis; structured questionnaire; stunting; underweight; vitamin intake; vitamin supplementation; World Health Organization; anemia; animal; Ascaris lumbricoides; growth disorder; helminthiasis; Indonesia; infant; malnutrition; parasitolo |
Public Library of Science |
19352727 |
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34138863 |
Article |
Q1 |
1990 |
1322 |
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350 |
Herawati F., Jaelani A.K., Wijono H., Rahem A., Setiasih, Yulia R., Andrajati R., Soemantri D. |
57194722742;57372559200;57222260570;57204655643;57209025002;56768083700;9940247700;36640659100; |
Antibiotic stewardship knowledge and belief differences among healthcare professionals in hospitals: A survey study |
2021 |
Heliyon |
7 |
6 |
e07377 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85109492428&doi=10.1016%2fj.heliyon.2021.e07377&partnerID=40&md5=5c399c28320a996c1e4e405a6c2d8169 |
Department of Clinical and Community Pharmacy, Faculty of Pharmacy, Universitas Surabaya, Surabaya, 60293, Indonesia; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia; Department of Pharmacy, RSUD Bangil, Pasuruan, 67153, Indonesia; Faculty of Medicine, Universitas Surabaya, Surabaya, 60293, Indonesia; Community Pharmacy Department, Faculty of Pharmacy, Airlangga University, Surabaya, 60115, Indonesia; Laboratory for Developmental Psychology, Faculty of Psychology, Universitas Surabaya, Surabaya, 60293, Indonesia; Department of Medical Education, Faculty of Medicine, Universitas Indonesia, Depok, 16424, Indonesia |
Herawati, F., Department of Clinical and Community Pharmacy, Faculty of Pharmacy, Universitas Surabaya, Surabaya, 60293, Indonesia, Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia; Jaelani, A.K., Department of Pharmacy, RSUD Bangil, Pasuruan, 67153, Indonesia; Wijono, H., Faculty of Medicine, Universitas Surabaya, Surabaya, 60293, Indonesia; Rahem, A., Community Pharmacy Department, Faculty of Pharmacy, Airlangga University, Surabaya, 60115, Indonesia; Setiasih, Laboratory for Developmental Psychology, Faculty of Psychology, Universitas Surabaya, Surabaya, 60293, Indonesia; Yulia, R., Department of Clinical and Community Pharmacy, Faculty of Pharmacy, Universitas Surabaya, Surabaya, 60293, Indonesia; Andrajati, R., Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia; Soemantri, D., Department of Medical Education, Faculty of Medicine, Universitas Indonesia, Depok, 16424, Indonesia |
Background: Collaborative practice in healthcare has been recommended to improve the quality of antimicrobial stewardship interventions, a behavioral change in antimicrobial use. Insufficient knowledge regarding antibiotic resistance, the fear of complications from infections, and how providers perceive antibiotic use and resistance are likely to influence prescribing behavior. This study's objective was to identify the knowledge and belief healthcare professionals' differences about antibiotic stewardship. Methods: This cross-sectional survey study of three hospitals in the East Java province, Indonesia utilized a 43-item questionnaire to assess antimicrobial stewardship knowledge and belief. There were 12 knowledge questions (total possible score: 12) and 31 belief questions (total possible score: 155). The Kuder Richardson 20 (KR-20) and Cronbach alpha values of the questionnaire were 0.54 and 0.92, respectively. Results: Out of the 257 respondents, 19% (48/257) had a low scores of knowledge, and 39% (101/257) had low scores on belief about antibiotic stewardship (101/257). Most midwives had a low scores on knowledge (25/61) and low scores on belief (46/61). Respondents with high scores on belief were 17% (10/59) physicians, 15% (4/27) pharmacists, 8% (5/65) nurses, and 3% (2/61) midwives. Conclusion: Among healthcare professionals, knowledge and belief differences concerning antibiotic stewardship vary widely. These differences will affect their capability, behavior, and contribution to the healthcare team collaboration and performance. Further studies are needed to evaluate the correlation between the level of inter-professional collaboration and the quality of the antibiotic stewardship implementation. © 2021 The Author(s) |
Antibiotic stewardship; Belief; Knowledge |
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Elsevier Ltd |
24058440 |
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Article |
Q1 |
455 |
10919 |
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352 |
Kristin E., Endarti D., Khoe L.C., Taroeno-Hariadi K.W., Trijayanti C., Armansyah A., Sastroasmoro S. |
6504458442;56626162000;56586245200;37012289000;57203727260;57225098604;6507794136; |
Economic Evaluation of Adding Bevacizumab to Chemotherapy for Metastatic Colorectal Cancer (mCRC) Patients in Indonesia |
2021 |
Asian Pacific Journal of Cancer Prevention |
22 |
6 |
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1921 |
1926 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85109162811&doi=10.31557%2fAPJCP.2021.22.6.1921&partnerID=40&md5=8b230f77f7dbd1e3652b63db9226b4c0 |
Department of Pharmacology and Therapy, Faculty of Medicine Public Health and Nursing, Gadjah Mada University, Yogyakarta, Indonesia; Department of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia; Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Respira Hospital, Yogyakarta, Indonesia; Center of Financing and Health Insurance, Ministry of Health, Government of Indonesia, Indonesia; Department of Pediatrics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Kristin, E., Department of Pharmacology and Therapy, Faculty of Medicine Public Health and Nursing, Gadjah Mada University, Yogyakarta, Indonesia; Endarti, D., Department of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia; Khoe, L.C., Department of Community Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Taroeno-Hariadi, K.W., Department of Pharmacology and Therapy, Faculty of Medicine Public Health and Nursing, Gadjah Mada University, Yogyakarta, Indonesia; Trijayanti, C., Respira Hospital, Yogyakarta, Indonesia; Armansyah, A., Center of Financing and Health Insurance, Ministry of Health, Government of Indonesia, Indonesia; Sastroasmoro, S., Department of Pediatrics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Objective: Since 2016, bevacizumab has been widely used to treat metastatic colorectal cancer (mCRC) in Indonesia. Nevertheless, the high cost of bevacizumab has raised the question of whether the therapy is considered cost-effective and should be included in the national health insurance system. This study aimed to assess the cost-effectiveness of bevacizumab plus chemotherapy versus chemotherapy alone for the treatment of mCRC patients. Methods: A Markov model was applied using the perspective of the Indonesian healthcare system to assess cost-effectiveness. The health outcomes were expressed in terms of quality-adjusted life years (QALY) using the validated EuroQoL-5D-5L instrument. Data for medical costs were collected from hospital billings in four hospitals located in three different cities in Indonesia. Meanwhile, data for utility were obtained from interviewing 90 patients who came to the hospital. We compared those mCRC patients who received chemotherapy alone either with FOLFOX or FOLFIRI, versus patients who received the addition of bevacizumab. Results: With the perspective of societal, the incremental cost-effectiveness ratio (ICER) of adding bevacizumab was USD 49,312 per QALY gained using secondary data and USD 28,446 per QALY using real world data. Conclusion: Using either a healthcare or societal perspective, the addition of bevacizumab for mCRC treatment was considered not cost-effective. © 2021. All Rights Reserved. |
Metastatic colorectal cancer- bevacizumab- chemotherapy |
antineoplastic agent; bevacizumab; camptothecin; fluorouracil; folinic acid; platinum complex; colorectal tumor; cost benefit analysis; economics; human; Indonesia; Markov chain; metastasis; pathology; quality adjusted life year; Antineoplastic Combined Chemotherapy Protocols; Bevacizumab; Camptothecin; Colorectal Neoplasms; Cost-Benefit Analysis; Fluorouracil; Humans; Indonesia; Leucovorin; Markov Chains; Neoplasm Metastasis; Organoplatinum Compounds; Quality-Adjusted Life Years |
Asian Pacific Organization for Cancer Prevention |
15137368 |
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34181352 |
Article |
Q2 |
512 |
9866 |
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354 |
Wangsaputra V.K., Syarinta S., Louisa M. |
57215576000;57224977663;41461551400; |
Alpha-mangostin Reduces Cell Viability in Sorafenib-surviving Cells by Modulating Multiple Drug Transporters in Hepg2 Hepatocellular Carcinoma Cells |
2021 |
Journal of Applied Pharmaceutical Science |
11 |
6 |
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105 |
110 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108813859&doi=10.7324%2fJAPS.2021.110612&partnerID=40&md5=eec4655af84852758d672bc24d8bc83b |
Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Department of Pharmacology, Faculty of Medicine, YARSI University, Jakarta, Indonesia; Department of Pharmacology and Therapeutics, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia |
Wangsaputra, V.K., Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; Syarinta, S., Department of Pharmacology, Faculty of Medicine, YARSI University, Jakarta, Indonesia; Louisa, M., Department of Pharmacology and Therapeutics, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia |
A previous study showed that alpha-mangostin (AM) showed benefit when given to sorafenib (SOR)-surviving cells. However, the mechanism was not fully understood. The present study aimed to understand the effect of AM on SOR-surviving cells and its agent concerning drug transporters. SOR-surviving cells were treated with SOR 10 μM. Surviving cells were divided into four groups of treatment, namely, vehicle only dimethyl sulfoxide (DMSO), SOR 10 μM, AM 20 μM, or combination of SOR 10 μM-AM 20 μM. As controls, HepG2 naïve cells were treated with DMSO only or AM 20 μM. Cell viability was counted using trypan blue exclusion assay. Simultaneously, the mRNA expressions of P-glycoprotein (P-gp), ABCG2, MRP2, MRP3, OCT1, and OATP1B3 drug transporters were examined with quantitative reverse transcriptase-polymerase chain reaction. Decreased mRNA expression of P-gp was found in SOR-surviving cells treated with SOR. In contrast, AM alone or SOR's combination caused a significant increase in both efflux and influx transporters, no difference in fold increase of all transporters evaluated in AM versus SOR-AM combinations. Generally, AM treatment increased the mRNA expression of all the drug transporters. © 2021 Vincent Kharisma Wangsaputra et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/). |
Alpha-mangostin; drug transporters; OCT1; P-glycoprotein; sorafenib resistance |
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Open Science Publishers LLP Inc. |
22313354 |
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Article |
Q2 |
286 |
15310 |
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356 |
Fadilah F., Erlina L., Paramita R.I., Istiadi K.A. |
56966708600;57190181680;54882436900;57224568113; |
Immunoinformatics Studies and Design of Breast Cancer Multiepitope Peptide Vaccines: Diversity Analysis Approach |
2021 |
Journal of Applied Pharmaceutical Science |
11 |
6 |
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035 |
045 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108795156&doi=10.7324%2fJAPS.2021.110604&partnerID=40&md5=36bb41265fd86ae6763c0ce748625cb9 |
Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Bioinformatics Core Facilities, Indonesian Medical Education and Research Institute IMERI, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Fadilah, F., Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Bioinformatics Core Facilities, Indonesian Medical Education and Research Institute IMERI, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Erlina, L., Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Bioinformatics Core Facilities, Indonesian Medical Education and Research Institute IMERI, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Paramita, R.I., Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Bioinformatics Core Facilities, Indonesian Medical Education and Research Institute IMERI, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Istiadi, K.A., Bioinformatics Core Facilities, Indonesian Medical Education and Research Institute IMERI, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Breast cancer is one of the most diagnosed cancers in women; the number of cases continues to rise. The high prevalence and increased incidence need more attention in developing effective therapy. Current passive therapy has several drawbacks that have not yet been resolved. Thus, an alternative and preventive therapy for cancer is needed by utilizing vaccines. Immunoinformatics approach is one of the promising methods predicting epitopes in vaccine research. This approach could accelerate the initial study process of vaccine development and reduce research costs. Epitope conservancy and vaccine coverage are important parameters in vaccine research due to addressing the variability and diversity of cancer genomics. This study will be carried out on the multiepitope characterization of potential T cells against the protein mechanism in breast cancer. Proteins used in this study are Mucin-4, Phosphatase And Tensin Homolog, and Receptor tyrosine-protein kinase erbB-2. CTL epitopes, antigenicity, immunogenicity, allergenicity, and toxicity were predicted for the peptide vaccine. Immunoinformatics analysis generates a multiepitope sequence consisting of seven epitopes: DPVALVAPF, SVAYRLGTL, SQINTLNTL, RFRELVSEF, VTSANIQEF, RPRFRELVS, and MYFEFPQPL by AAY linker. The docking and molecular dynamics analyses were conducted to confirm the interactions between the multiepitope vaccine molecule and TLR-4-MD. The multiepitope vaccine construct can be an appropriate choice for further experiments. © 2021 Fadilah Fadilah et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/). |
Breast cancer; immunoinformatics; multiepitope; peptide vaccine design; vaccine |
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Open Science Publishers LLP Inc. |
22313354 |
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Article |
Q2 |
286 |
15310 |
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361 |
Sasmono R.T., Johar E., Yohan B., Ma'Roef C.N., Pronyk P., Hadinegoro S.R., Soepardi E.J., Bouckenooghe A., Hawley W.A., Rosenberg R., Powers A.M., Soebandrio A., Myint K.S.A. |
6506482032;57204001174;55843037500;6507740388;6602466584;57226218772;57191174412;18233281300;7004280510;35578810900;7005770718;8602893200;7003758970; |
Spatiotemporal heterogeneity of zika virus transmission in indonesia: Serosurveillance data from a pediatric population |
2021 |
American Journal of Tropical Medicine and Hygiene |
104 |
6 |
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2220 |
2223 |
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4 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107590950&doi=10.4269%2fajtmh.21-0010&partnerID=40&md5=31e9b723a56e45315c1b9d58055ee86b |
Eijkman Institute for Molecular Biology, Jakarta, Indonesia; UNICEF Indonesia, Jakarta, Indonesia; Faculty of Medicine and Cipto Mangunkusumo Hospital, Universitas Indonesia, Jakarta, Indonesia; Ministry of Health of the Republic of Indonesia, Jakarta, Indonesia; Sanofi Pasteur, Lyon, Rhône-Alpes, France; Centers for Disease Control and Prevention, Atlanta, GA, United States; Centers for DiseaseControl and Prevention, Fort Collins, CO, United States |
Sasmono, R.T., Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Johar, E., Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Yohan, B., Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Ma'Roef, C.N., Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Pronyk, P., UNICEF Indonesia, Jakarta, Indonesia; Hadinegoro, S.R., Faculty of Medicine and Cipto Mangunkusumo Hospital, Universitas Indonesia, Jakarta, Indonesia; Soepardi, E.J., Ministry of Health of the Republic of Indonesia, Jakarta, Indonesia; Bouckenooghe, A., Sanofi Pasteur, Lyon, Rhône-Alpes, France; Hawley, W.A., Centers for Disease Control and Prevention, Atlanta, GA, United States; Rosenberg, R., Centers for DiseaseControl and Prevention, Fort Collins, CO, United States; Powers, A.M., Centers for DiseaseControl and Prevention, Fort Collins, CO, United States; Soebandrio, A., Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Myint, K.S.A., Eijkman Institute for Molecular Biology, Jakarta, Indonesia |
The presence of Zika virus (ZIKV) in Indonesia has been recognized since the 1970s, but its transmission dynamics there have been poorly understood. To understand more fully the geographic distribution and burden of ZIKV infection, we performed retrospective serological tests on specimens collected from asymptomatic children age 5 to 9 years old living at 30 sites in 14 provinces. Of 870 serum samples tested, 9.2% were found to be positive for anti-ZIKV antibodies, as confirmed by plaque reduction neutralization assays. This was the same overall prevalence reported previously for 1- to 4-year-old children collected at the same sites at the same time. Together with geographic differences in seroprevalence between the age groups, these data suggest that, although ZIKV might be endemic in Indonesia, its occurrence has been focal and episodic. © 2021 by The American Society of Tropical Medicine and Hygiene. |
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Article; asymptomatic disease; blood sampling; disease burden; geographic distribution; human; Indonesia; nonhuman; plaque reduction neutralization test; serological surveillance; serology; seroprevalence; virus transmission; Zika fever; Zika virus; blood; child; epidemiological monitoring; immunology; preschool child; retrospective study; seroepidemiology; spatiotemporal analysis; Zika fever; Zika virus; immunoglobulin M; virus antibody; Antibodies, Viral; Child; Child, Preschool; Epidemiological Monitoring; Humans; Immunoglobulin M; Indonesia; Retrospective Studies; Seroepidemiologic Studies; Spatio-Temporal Analysis; Zika Virus; Zika Virus Infection |
American Society of Tropical Medicine and Hygiene |
00029637 |
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33939632 |
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Q1 |
1015 |
4298 |
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369 |
Yugo D., Lo L.-W., Wu Y.-H., Chung F.-P., Lin Y.-J., Chang S.-L., Hu Y.-F., Chao T.-F., Liao J.-N., Chang T.-Y., Lin C.-Y., Tuan T.-C., Kuo L., Wu C.-I., Liu C.-M., Liu S.-H., Cheng W.-H., Lugtu I.C., Jain A., Chen S.-A. |
57215499689;25224685100;57150405500;40560956700;56512618600;57154909400;57219519883;35335897300;55179923000;56763173800;56469967500;7005704090;57193883698;56320807100;57202736753;57191375309;26025932500;57212467736;57216788165;57211727582; |
Case series on stereotactic body radiation therapy in non-ischemic cardiomyopathy patients with recurrent ventricular tachycardia |
2021 |
PACE - Pacing and Clinical Electrophysiology |
44 |
6 |
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1085 |
1093 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105961876&doi=10.1111%2fpace.14254&partnerID=40&md5=d3ccf6f5cafcbd0b6064e6a5a180d5ce |
Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; Department of Cardiology and Vascular Medicine, Faculty of Medicine University of Indonesia, Jakarta, Indonesia; Arrhythmia Division, Pusat Jantung Nasional Harapan Kita, Jakarta, Indonesia; Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Division of Radiation Oncology, Department of Oncology, Taipei Veterans General Hospital, Taipei, Taiwan; Department of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Department of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan; Heart Institute, Chinese General Hospital and Medical Center, Metro Manila, Philippines; Department of Cardiology, Vardhman Mahavir Medical College, New Delhi, India; Cardiovascular Center, Taichung Veterans General Hospital, Taichung, Taiwan |
Yugo, D., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Department of Cardiology and Vascular Medicine, Faculty of Medicine University of Indonesia, Jakarta, Indonesia, Arrhythmia Division, Pusat Jantung Nasional Harapan Kita, Jakarta, Indonesia; Lo, L.-W., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Wu, Y.-H., Division of Radiation Oncology, Department of Oncology, Taipei Veterans General Hospital, Taipei, Taiwan, Department of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan, Department of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan; Chung, F.-P., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Lin, Y.-J., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Chang, S.-L., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Hu, Y.-F., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Chao, T.-F., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Liao, J.-N., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Chang, T.-Y., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Lin, C.-Y., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Tuan, T.-C., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Kuo, L., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Wu, C.-I., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Liu, C.-M., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Liu, S.-H., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Cheng, W.-H., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Lugtu, I.C., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Heart Institute, Chinese General Hospital and Medical Center, Metro Manila, Philippines; Jain, A., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Department of Cardiology, Vardhman Mahavir Medical College, New Delhi, India; Chen, S.-A., Heart Rhythm Center, Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, Faculty of Medicine and Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan, Cardiovascular Center, Taichung Veterans General Hospital, Taichung, Taiwan |
Introduction: The efficacy of stereotactic body radiation therapy (SBRT) as an alternative treatment for recurrent ventricular tachycardia (VT) is still unclear. This study aimed to report the outcome of SBRT in VT patients with nonischemic cardiomyopathy (NICM). Methods: The determination of the target substrate for radiation was based on the combination of CMR results and electroanatomical mapping merged with the real-time CT scan image. Radiation therapy was performed by Flattening-filter-free (Truebeam) system, and afterward, patients were followed up for 13.5 ± 2.8 months. We analyzed the outcome of death, incidence of recurrent VT, ICD shocks, anti-tachycardia pacing (ATP) sequences, and possible irradiation side-effects. Results: A total of three cases of NICM patients with anteroseptal scar detected by CMR. SBRT was successfully performed in all patients. During the follow-up, we found that VT recurrences occurred in all patients. In one patient, it happened during a 6-week blanking period, while the others happened afterward. Re-hospitalization due to VT only appeared in one patient. Through ICD interrogation, we found that all patients have reduced VT burden and ATP therapies. All of the patients died during the follow-up period. Radiotherapy-related adverse events did not occur in all patients. Conclusions: SBRT therapy reduces the number of VT burden and ATP sequence therapy in NICM patients with VT, which had a failed previous catheter ablation. However, the efficacy and safety aspects, especially in NICM cases, remained unclear. © 2021 Wiley Periodicals LLC |
radio-ablation; stereotactic; ventricular tachycardia |
aged; Article; cardiovascular magnetic resonance; case report; clinical article; clinical effectiveness; clinical outcome; computer assisted tomography; death; disease burden; female; follow up; heart pacing; heart ventricle tachycardia; hospital readmission; human; incidence; male; nonischemic cardiomyopathy; recurrent disease; stereotactic body radiation therapy; very elderly; cardiomyopathy; diagnostic imaging; epicardium mapping; heart ventricle tachycardia; procedures; radiosurgery; radiotherapy dosage; scar; x-ray computed tomography; Aged; Aged, 80 and over; Cardiomyopathies; Cicatrix; Epicardial Mapping; Female; Humans; Male; Radiosurgery; Radiotherapy Dosage; Tachycardia, Ventricular; Tomography, X-Ray Computed |
John Wiley and Sons Inc |
01478389 |
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33932305 |
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686 |
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378 |
Mori Y., Sato H., Kumazawa T., Mayang Permata T.B., Yoshimoto Y., Murata K., Noda S.-E., Kaminuma T., Ando K., Oike T., Okonogi N., Okada K., Kakoti S., Suzuki K., Ikota H., Yokoo H., Nakano T., Ohno T., Shibata A. |
57210846989;55697961900;57210432294;57197808751;36453407100;36103294900;14621772700;23994005700;55641963900;36453136000;36453127400;57222984111;57197814645;57376271900;57214213081;55588986400;35353843800;35395665700;8323572900; |
Analysis of radiotherapy-induced alteration of CD8+ T cells and PD-L1 expression in patients with uterine cervical squamous cell carcinoma |
2021 |
Oncology Letters |
21 |
6 |
446 |
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2 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104336493&doi=10.3892%2fol.2021.12707&partnerID=40&md5=a047e0e92118f6cb0de5b48cfc613e96 |
Department of Radiation Oncology, Graduate School of Medicine, Gunma University, Maebashi, Gunma, 371-8511, Japan; Department of Radiotherapy, Saitama Cancer Center, Ina, Saitama, 362-0806, Japan; Department of Radiation Oncology, Faculty of Medicine Universitas Indonesia, Dr Cipto Mangunkusumo Hospital, Jakarta, 10430, Indonesia; Department of Radiation Oncology, Fukushima Medical University, Fukushima, 960-1247, Japan; Department of Radiation Oncology, Comprehensive Cancer Center, International Medical Center, Saitama Medical University, Hidaka, Saitama, 350-1298, Japan; National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan; Signal Transduction Program, Gunma University Initiative for Advanced Research, Gunma University, Maebashi, Gunma, 371-8511, Japan; Department of Radiation Medical Sciences, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, 852-8523, Japan; Clinical Department of Pathology, Gunma University Hospital, Maebashi, Gunma, 371-8511, Japan; Department of Human Pathology, Gunma University, Graduate School of Medicine, Maebashi, Gunma, 371-8511, Japan |
Mori, Y., Department of Radiation Oncology, Graduate School of Medicine, Gunma University, Maebashi, Gunma, 371-8511, Japan, Department of Radiotherapy, Saitama Cancer Center, Ina, Saitama, 362-0806, Japan; Sato, H., Department of Radiation Oncology, Graduate School of Medicine, Gunma University, Maebashi, Gunma, 371-8511, Japan; Kumazawa, T., Department of Radiation Oncology, Graduate School of Medicine, Gunma University, Maebashi, Gunma, 371-8511, Japan; Mayang Permata, T.B., Department of Radiation Oncology, Faculty of Medicine Universitas Indonesia, Dr Cipto Mangunkusumo Hospital, Jakarta, 10430, Indonesia; Yoshimoto, Y., Department of Radiation Oncology, Fukushima Medical University, Fukushima, 960-1247, Japan; Murata, K., Department of Radiation Oncology, Graduate School of Medicine, Gunma University, Maebashi, Gunma, 371-8511, Japan; Noda, S.-E., Department of Radiation Oncology, Comprehensive Cancer Center, International Medical Center, Saitama Medical University, Hidaka, Saitama, 350-1298, Japan; Kaminuma, T., Department of Radiation Oncology, Graduate School of Medicine, Gunma University, Maebashi, Gunma, 371-8511, Japan; Ando, K., Department of Radiation Oncology, Graduate School of Medicine, Gunma University, Maebashi, Gunma, 371-8511, Japan; Oike, T., Department of Radiation Oncology, Graduate School of Medicine, Gunma University, Maebashi, Gunma, 371-8511, Japan; Okonogi, N., National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan; Okada, K., Department of Radiation Oncology, Graduate School of Medicine, Gunma University, Maebashi, Gunma, 371-8511, Japan; Kakoti, S., Department of Radiation Oncology, Graduate School of Medicine, Gunma University, Maebashi, Gunma, 371-8511, Japan, Signal Transduction Program, Gunma University Initiative for Advanced Research, Gunma University, Maebashi, Gunma, 371-8511, Japan; Suzuki, K., Department of Radiation Medical Sciences, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, 852-8523, Japan; Ikota, H., Clinical Department of Pathology, Gunma University Hospital, Maebashi, Gunma, 371-8511, Japan; Yokoo, H., Department of Human Pathology, Gunma University, Graduate School of Medicine, Maebashi, Gunma, 371-8511, Japan; Nakano, T., National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan; Ohno, T., Department of Radiation Oncology, Graduate School of Medicine, Gunma University, Maebashi, Gunma, 371-8511, Japan; Shibata, A., Signal Transduction Program, Gunma University Initiative for Advanced Research, Gunma University, Maebashi, Gunma, 371-8511, Japan |
Radiotherapy induces an immune response in the cancer microenvironment that may influence clinical outcome. The present study aimed to analyse the alteration of CD8+ T-cell infiltration and programmed death-ligand 1 (PD-L1) expression following radiotherapy in clinical samples from patients with uterine cervical squamous cell carcinoma. Additionally, the current study sought to analyse the associa- tion between these immune responses and clinical outcomes. A total of 75 patients who received either definitive chemoradio- therapy or radiotherapy were retrospectively analyzed. CD8+ T-cell infiltration and PD-L1 expression were determined by immunohistochemistry using biopsy specimens before radio- therapy (pre-RT) and after 10 Gy radiotherapy (post-10 Gy). The PD-L1+ rate was significantly increased from 5% (4/75) pre-RT to 52% (39/75) post-10 Gy (P<0.01). Despite this increase in the PD-L1+ rate post-10 Gy, there was no significant association between both pre-RT and post-10 Gy and overall survival (OS), locoregional control (LC) and progression-free survival (PFS). On the other hand, the CD8+ T-cell infiltration density was significantly decreased for all patients (median, 23.1% pre-RT vs. 16.9% post-10 Gy; P=0.038); however, this tended to increase in patients treated with radiotherapy alone (median, 17.7% pre-RT vs. 24.0% post-10 Gy; P=0.400). Notably, patients with high CD8+ T-cell infiltration either pre-RT or post-10 Gy exhibited positive associations with OS, LC and PFS. Thus, the present analysis suggested that CD8+ T-cell infiltration may be a prognostic biomarker for patients with cervical cancer receiving radiotherapy. Furthermore, immune checkpoint inhibitors may be effective in patients who have received radiotherapy, since radiotherapy upregu- lated PD-L1 expression in cervical cancer specimens. © 2021 Spandidos Publications. All rights reserved. |
CD8+ T cell; Cervical cancer; Immune modulation; Programmed death-ligand 1; Radiotherapy; Tumor microenvironment |
alcohol; biological marker; biotin; cisplatin; citric acid; diaminobenzidine; edetic acid; formaldehyde; hydrogen peroxide; immune checkpoint inhibitor; paraffin; peroxidase; platinum; programmed death 1 ligand 1; streptavidin; adult; aged; antigen retrieval; Article; biopsy; brachytherapy; cancer radiotherapy; cancer staging; CD8+ T lymphocyte; cell density; cell infiltration; chemoradiotherapy; controlled study; diagnostic test accuracy study; down regulation; female; follow up; human; human tissue; immune response; immunohistochemistry; irradiation; lymph node metastasis; major clinical study; microscopy; overall survival; paraffin embedding; progression free survival; protein expression; receiver operating characteristic; retrospective study; room temperature; tumor associated leukocyt |
Spandidos Publications |
17921074 |
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6367 |
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383 |
Pranata R., Henrina J., Yonas E., Putra I.C.S., Cahyadi I., Lim M.A., Munawar D.A., Munawar M. |
57201973901;57218482646;57201987097;57222144236;57221688594;57216039756;56470745000;16747447600; |
BMI and atrial fibrillation recurrence post catheter ablation: A dose-response meta-analysis |
2021 |
European Journal of Clinical Investigation |
51 |
6 |
e13499 |
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3 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101961208&doi=10.1111%2feci.13499&partnerID=40&md5=72dba8a2a9279126fd9e453324e6df70 |
Faculty of Medicine, Universitas Pelita Harapan, Tangerang, Indonesia; Binawaluya Cardiac Center, Jakarta, Indonesia; Balaraja General Hospital, Tangerang, Indonesia; Faculty of Medicine, Universitas YARSI, Jakarta, Indonesia; Syamsudin SH General Hospital, Sukabumi, Indonesia; Pasar Rebo General Hospital, Jakarta, Indonesia; Department of Cardiology, Lyell McEwin Hospital, University of Adelaide, Elizabeth Vale, Australia; Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Pranata, R., Faculty of Medicine, Universitas Pelita Harapan, Tangerang, Indonesia, Binawaluya Cardiac Center, Jakarta, Indonesia; Henrina, J., Balaraja General Hospital, Tangerang, Indonesia; Yonas, E., Faculty of Medicine, Universitas YARSI, Jakarta, Indonesia; Putra, I.C.S., Syamsudin SH General Hospital, Sukabumi, Indonesia; Cahyadi, I., Pasar Rebo General Hospital, Jakarta, Indonesia; Lim, M.A., Faculty of Medicine, Universitas Pelita Harapan, Tangerang, Indonesia; Munawar, D.A., Department of Cardiology, Lyell McEwin Hospital, University of Adelaide, Elizabeth Vale, Australia, Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Munawar, M., Binawaluya Cardiac Center, Jakarta, Indonesia, Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia |
Introduction: The evidence on the association between obesity and atrial fibrillation (AF) recurrence was equivocal. We aimed to evaluate the dose-response relationship between body mass index (BMI) and AF recurrence and adverse events. Methods: A systematic literature search was conducted using PubMed, Europe PMC, EBSCO, ProQuest and Cochrane Library. Obesity was defined as BMI ≥28 kg/m2. The primary outcome was AF recurrence, and the secondary outcome was adverse events. Adverse events were defined as procedure-related complications and cardio-cerebrovascular events. Results: There were a total of 52,771 patients from 20 studies. Obesity was associated with higher AF recurrence (Odds ratio [OR] 1.30 [95% confidence interval [CI] 1.16-1.47], P <.001; I2: 72.7%) and similar rate of adverse events (OR 1.21 [95% CI 0.87-1.67], P =.264; I2: 23.9%). Meta-regression showed that the association varies by age (coefficient: −0.03, P =.024). Meta-analysis of highest versus lowest BMI showed that the highest group had higher AF recurrence (OR 1.37 [95% CI 1.18-1.58], P <.001; I2: 64.9%) and adverse events (OR 2.02 [95% CI 1.08-3.76], P =.028; I2: 49.5%). The linear association analysis for AF recurrence was not significant (P =.544). The dose-response relationship for BMI and AF recurrence was nonlinear (pnonlinearity < 0.001), the curve became steeper at 30-35 kg/m2. For adverse events, an increase of 1% for every 1 kg/m2 increase in BMI (OR 1.01 [95% CI 1.00-1.02], P =.001), the relationship was nonlinear (pnonlinearity = 0.001). Conclusion: Obesity was associated with higher AF recurrence in patients undergoing catheter ablation. High BMI might be associated with a higher risk for adverse events. PROSPERO ID: CRD42020198787. © 2021 Stichting European Society for Clinical Investigation Journal Foundation. Published by John Wiley & Sons Ltd |
arrhythmia; Atrial Fibrillation; catheter ablation; obesity; pulmonary vein isolation |
anticoagulant agent; warfarin; adult; adverse event; age; aged; Article; atrial fibrillation; body mass; cardiovascular disease; catheter ablation; cerebrovascular disease; female; high risk patient; human; male; meta analysis; middle aged; obesity; outcome assessment; recurrence risk; systematic review; atrial fibrillation; body mass; obesity; recurrent disease; severity of illness index; Atrial Fibrillation; Body Mass Index; Catheter Ablation; Humans; Obesity; Overweight; Recurrence; Severity of Illness Index |
John Wiley and Sons Inc |
00142972 |
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33544873 |
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1164 |
3461 |
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Bangun K., Sukasah C.L., Dilogo I.H., Indrani D.J., Siregar N.C., Pandelaki J., Iskandriati D., Kekalih A., Halim J. |
36902624600;57218391534;56161962800;6602774472;6508087790;35759266900;6507742011;55633562200;57219398869; |
Bone Growth Capacity of Human Umbilical Cord Mesenchymal Stem Cells and BMP-2 Seeded Into Hydroxyapatite/Chitosan/Gelatin Scaffold in Alveolar Cleft Defects: An Experimental Study in Goat |
2021 |
Cleft Palate-Craniofacial Journal |
58 |
6 |
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707 |
717 |
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1 |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092575595&doi=10.1177%2f1055665620962360&partnerID=40&md5=a3280d360d1de28c8353dffc23d716b3 |
Department of Plastic and Reconstructive Surgery, Cleft and Craniofacial Center, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Unit Pelayanan Terpadu Teknologi Kedokteran Sel Punca (Stem Cell Research Center), Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Department of Dental Material Science and Technology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia; Anatomical Pathology Department, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Radiology Department of Cipto, Mangunkusumo General Hospital, Jakarta, Indonesia; Primate Research Center of Bogor Agricultural Institute, Bogor, Indonesia; Community Medicine Department, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Cipto Mangunkusumo General Hospital, Jakarta, Indonesia |
Bangun, K., Department of Plastic and Reconstructive Surgery, Cleft and Craniofacial Center, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Sukasah, C.L., Department of Plastic and Reconstructive Surgery, Cleft and Craniofacial Center, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Dilogo, I.H., Unit Pelayanan Terpadu Teknologi Kedokteran Sel Punca (Stem Cell Research Center), Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Indrani, D.J., Department of Dental Material Science and Technology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia; Siregar, N.C., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Anatomical Pathology Department, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Pandelaki, J., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Radiology Department of Cipto, Mangunkusumo General Hospital, Jakarta, Indonesia; Iskandriati, D., Primate Research Center of Bogor Agricultural Institute, Bogor, Indonesia; Kekalih, A., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Community Medicine Department, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; Halim, J., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia |
Objective: To evaluate bone regeneration in alveolar defects treated with human umbilical cord–derived mesenchymal stem cells (hUCMSCs), hydroxyapatite/chitosan/gelatin (HA/CS/Gel) scaffold, and bone morphogenic protein-2 (BMP-2) in Capra hircus models. Design: Randomized posttest-only control group design. Setting: Animal Hospital at Bogor Agricultural Institute. Participants: Healthy and equally treated 24 female Capra hircus/goats. Intervention: Animals were randomly assigned to 3 experimental group design (iliac crest alveolar bone graft/ICABG [control], HA/Cs/Gel+BMP-2 [Novosys], and HA/Cs/Gel+BMP-2+UCMSCs). Graft materials were implanted in surgically made alveolar defects. Main Outcome Measures: Postoperative functional score and operating time were assessed. New bone growth, bone density, inflammatory cells recruitment, and neoangiogenesis were evaluated based on radiological and histological approach at 2 time points, week 4 and 12. Statistical analysis was done between treatment groups. Results: Operating time was 34% faster and functional score 94.5% more superior in HA/Cs/Gel+BMP-2+hUCMSC group. Bone growth capacity in HA/Cs/Gel+BMP-2+UCMSCs mimicked ICABG, but ICABG showed possibility of bone loss between week 4 and 12. The HA/Cs/Gel+BMP-2+UCMSCs showed early bone repopulation and unseen inflammatory cells and angiogenesis on week 12. Discussion and Conclusion: The HA/Cs/Gel+BMP-2+hUCMSCs were superior in enhancing new bone growth without donor site morbidity compared to ICABG. The presence of hUCMSCs in tissue-engineered alveolar bone graft (ABG), supported with paracrine activity of the resident stem cells, initiated earlier new bone repopulation, and completed faster bone regeneration. The HA/Cs/Gel scaffold seeded with UCMSCs+BMP-2 is a safe substitute of ICABG to close alveolar bone defects suitable for patients with cleft lip, alveolus, and palate. © American Cleft Palate-Craniofacial Association. All rights reserved 2020. |
alveolar bone grafts; bone regeneration; hydroxyapatite/chitosan/gelatin scaffold; iliac crest bone graft; mesenchymal stem cells; tissue engineering |
bone morphogenetic protein 2; chitosan; gelatin; hydroxyapatite; gelatin; hydroxyapatite; adult; alveolar bone; alveolar bone grafting; angiogenesis; animal experiment; animal model; animal tissue; Article; bone density; bone graft; bone growth; bone regeneration; controlled study; domestic goat; donor site; female; histology; human; human cell; iliac crest; inflammatory cell; jaw malformation; morbidity; nonhuman; operation duration; osteolysis; outcome assessment; paracrine signaling; umbilical cord mesenchymal stem cell; animal; bone development; goat; mesenchymal stem cell; tissue scaffold; umbilical cord; Animals; Bone Development; Bone Regeneration; Chitosan; Durapatite; Female; Gelatin; Goats; Humans; Mesenchymal Stem Cells; Tissue Scaffolds; Umbilical Cord |
SAGE Publications Ltd |
10556656 |
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