709 |
Ayu Asri Prima Dewi A.A., Antarianto R.D., Pawitan J.A. |
57268721600;57190862806;6508348067; |
Characterization of indonesia decellularized liver cubes scaffold using scanning electron microscopy |
2021 |
Journal of Biomimetics, Biomaterials and Biomedical Engineering |
52 |
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38 |
46 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115446740&doi=10.4028%2fwww.scientific.net%2fJBBBE.52.38&partnerID=40&md5=c7fc1ea62d3680df39cd8c77bf963ba1 |
Master Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430, Indonesia; Department of Histology, Faculty of Medicine, Warmadewa University, Jl. Terompong 24 Tanjung Bungkak, Denpasar, Bali, Indonesia; Department of Histology, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430, Indonesia; Stem Cell and Tissue Engineering Research Cluster, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430, Indonesia; Integrated Stem Cell Technology Service Unit, Cipto Mangunkusumo National General Hospital, Jl. Diponegoro No.71, Salemba, Jakarta, Indonesia |
Ayu Asri Prima Dewi, A.A., Master Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430, Indonesia, Department of Histology, Faculty of Medicine, Warmadewa University, Jl. Terompong 24 Tanjung Bungkak, Denpasar, Bali, Indonesia; Antarianto, R.D., Department of Histology, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430, Indonesia, Stem Cell and Tissue Engineering Research Cluster, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430, Indonesia; Pawitan, J.A., Department of Histology, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430, Indonesia, Stem Cell and Tissue Engineering Research Cluster, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430, Indonesia, Integrated Stem Cell Technology Service Unit, Cipto Mangunkusumo National General Hospital, Jl. Diponegoro No.71, Salemba, Jakarta, Indonesia |
Liver biological scaffold was developed in order to resemble native liver tissue environment. It can be achieved by decellularizing native liver tissue that will remove cells and preserve extracellular matrix (ECM). Furthermore, ECM fibers are arranged in a special pattern, which affect liver cell polarity and topography that are important for cells’ implantation, proliferation and differentiation. Therefore, the aim of this study was to evaluate liver cube scaffold topography that was decellularized with fixed multiple sites syringe injection (Indonesia patent number: S00201907930). Rat liver cubes (n=3) underwent decellularization with Ethylene Glycol Tetraacetic Acid (EGTA) immersion and increased Sodium Dodecyl Sulfate (SDS) concentrations using previous multiple sites syringe injection protocol study. Deoxyribonucleic Acid (DNA) concentrations were measured to confirm less DNA materials remaining in scaffolds. Scanning Electron Microscope (SEM) analysis of scaffolds were conducted for topographic characterization compared to undecellularized liver control. Molecular analysis of DNA concentration showed complete removal of DNA material. SEM analysis gave appearance of intact liver cube scaffold microarchitecture. Liver cubes decellularization using multiple sites syringe injection showed good topographic liver scaffold characterization. © 2021 Trans Tech Publications Ltd, Switzerland. |
Liver biological scaffold; Liver cube tissue; Liver decellularization; Multiple site syringe injection; Scanning electron microscope |
Cytology; DNA; Ethylene; Ethylene glycol; Geometry; Scaffolds (biology); Sodium dodecyl sulfate; Sulfur compounds; Syringes; Tissue; Acid concentrations; Biological scaffolds; Decellularization; Extracellular matrices; Indonesia; Liver biological scaffold; Liver cube tissue; Liver decellularization; Liver tissue; Multiple site syringe injection; Scanning electron microscopy |
Trans Tech Publications Ltd |
22969837 |
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Article |
Q4 |
183 |
20493 |
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