Publikasi Scopus FKUI 2021 per tanggal 31 Juli 2021 (507 artikel)

Liem I.K., Oktavina R., Zakiyah, Anggraini D., Deraya I.E., Kodariah R., Krisnuhoni E., Wuyung P.E.
55802927800;57224993301;57224999441;57225000673;57216313121;14010667100;35310982200;57192889605;
Intravenous injection of umbilical cord-derived mesenchymal stem cells improved regeneration of rat liver after 2aaf/ccl4-induced injury
2021
OnLine Journal of Biological Sciences
21
2
317
326
Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo Central Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Stem Cell and Tissue Engineering Cluster, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Integrated Laboratory, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Anatomy, Faculty of Medicine and Health Science, Universitas Kristen Krida Wacana, Jakarta, Indonesia; Department of Anatomy, Faculty of Medicine, Universitas Yarsi, Jakarta, Indonesia; Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Animal Research Facilities, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Liem, I.K., Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo Central Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Stem Cell and Tissue Engineering Cluster, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Integrated Laboratory, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Oktavina, R., Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Department of Anatomy, Faculty of Medicine and Health Science, Universitas Kristen Krida Wacana, Jakarta, Indonesia; Zakiyah, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Department of Anatomy, Faculty of Medicine, Universitas Yarsi, Jakarta, Indonesia; Anggraini, D., Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Stem Cell and Tissue Engineering Cluster, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Deraya, I.E., Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Kodariah, R., Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Krisnuhoni, E., Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Wuyung, P.E., Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Animal Research Facilities, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
The effectivity of Mesenchymal Stem Cell (MSC) therapy for chronic liver injury is still questioned due to its possibility to have a pro-fibrotic effects. Other consideration is the preferably peripheral Intravenous (IV) route of application rather than direct to the target organ, such as portal vein or splenic vein, since the most of MSC will be trapped in the lung and other organs. Therefore, a study has been carried out in an animal model of chronic liver injury, 2AAF/CCl4 rats, to find out whether IV injection of single dose, 1×106 human umbilical cord-derived MSC (hUC-MSC) can regenerate the liver tissue and reduce the fibrosis. Male Wistar rats (n = 18, 8 weeks, 160-200 g) were divided into three groups of six rats, i.e., group I (control/healthy rats), group II (2AAF/CCl4-induced liver injury with hUC-MSC injected rats) and group III (2AAF/CCl4-incuded liver injury without hUC-MSC injected rats). Gross anatomical features of the livers and blood examination were tested. There was no significant different of the Alanine Aminotransferase (ALT) and Albumin serum level between groups; however there was a tendency of ALT decrease and Albumin increase in stem cells treated rats (group II). Histopathological observation using hematoxylin eosin and Masson’s trichrome staining showed an improvement in the liver damage condition (reduction of necrosis and coverage area of fat degeneration) and an improvement in the fibrosis condition in the group II compared to the group III. None of group II rat had fallen into cirrhosis as seen in group III. Immunohistochemistry staining against Caspase 3 (biomarker for apoptosis) also showed significant reduction (p = 0.038; Tukey multiple comparison test) of group II expression index (2.74%) compared to the group III (8.64%). In conclusion, IV injection of single dose, 1×106 hUC-MSC could regenerate the liver tissue and reduce the fibrosis in 2AAF/CCl4 rats model of chronic liver injury. © 2021 Isabella Kurnia Liem, Reni Oktavina, Zakiyah, Dian Anggraini, Irwina Eka Deraya, Ria Kodariah, Ening Krisnuhoni, and Puspita Eka Wuyung.
2AAF/CCl4; Caspase 3; Liver Fibrosis; Liver Injury; Liver Regeneration; MSC
Science Publications
16084217
Article
Q4
152
23000