Publikasi Scopus FKUI 2021 per tanggal 31 Juli 2021 (507 artikel)

Document details - Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
Huang, R., Chongqing University, School of Medicine, Center for Neurointelligence, Chongqing, China; Huang, S., The Second Affiliated Hospital of Xi'an Jiaotong University, Department of Radiation Oncology, Xi'an, Shaan Xi, China; Huang, T., Northwestern University Feinberg School of Medicine, Department of Neurology & Lou & Jean Malnati Brain Tumor Institute, Chicago, IL, United States; Huang, X., Zhejiang University School of Medicine, The First Affiliated Hospital, Zhejiang Provincial Key Laboratory of Pancreatic Disease, Hangzhou, Zhejiang, China; Huang, Y.J., Life Sciences Institute and Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, United States; Huber, T.B., III. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Hubert, V., Medical University of Vienna, Department of Pathology, Vienna, Austria; Hubner, C.A., University Hospital Jena, Institute of Human Genetics, Jena, Thuringia, Germany; Hughes, S.M., University of Otago, School of Biomedical Sciences and Brain Health Research Centre, Department of Biochemistry, Dunedin, New Zealand; Hughes, W.E., Medical College of Wisconsin, Department of Medicine and Cardiovascular Center, Milwaukee, WI, United States; Humbert, M., University of Bern, Institute of Pathology, Bern, Switzerland; Hummer, G., Max Planck Institute of Biophysics, Department of Theoretical Biophysics, Frankfurt am Main, Germany; Hurley, J.H., University of California, Berkeley, Department of Molecular and Cell Biology, Berkeley, CA, United States; Hussain, S., McGill University Health Centre, Montréal, QC, Canada; Hussain, S., West Virginia University, School of Medicine, Department of Physiology and Pharmacology, Morgantown, WV, United States; Hussey, P.J., Durham University, Department of Biosciences, Durham, United Kingdom; Hutabarat, M., University of Indonesia, Faculty of Medicine, Department of Obstetric and Gynecology, Jakarta, Indonesia;
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field. © 2020 Informa UK Limited, trading as Taylor & Francis Group.
Autophagosome; cancer; flux; LC3; lysosome; macroautophagy; neurodegeneration; phagophore; stress; vacuole
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