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Publikasi Scopus FKUI 2021 per tanggal 30 Juni 2021 (428 artikel)
Publikasi Scopus FKUI 2021 per tanggal 30 Juni 2021 (428 artikel)
Comparison between PRP and PRFM on FTSG healing profile: Macroscopic, microscopic and ELISA evaluation
2021
Annals of Medicine and Surgery
66
102350
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105502675&doi=10.1016%2fj.amsu.2021.102350&partnerID=40&md5=632e6c1f802e9603336c9a0770ecc410
Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Department of Pathology Anatomy, Faculty of Medicine, Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; Eijkman Institute for Molecular Biology, Jakarta, Indonesia; Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, University of Padjajaran, Hasan Sadikin Hospital, Bandung, Indonesia; Faculty of Public Health, Centre for Health Administration, Management and Policy, Universitas Indonesia, Jakarta, Indonesia; Department of Clinics, Reproduction, and Pathology, Faculty of Veterinary Medicine, Institute Pertanian Bogor, Bogor, Indonesia; Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia
Background: Studies had shown the benefit of PRFM and PRP in wound healing but their use in skin graft healing was rarely studied. This study aims to compare the use of PRP and PRFM in accelerating wound healing process of skin full-thickness skin graft (FTSG). Materials and methods: Five pigs were used to look at the wound healing effect of PRP and PRFM usage prior to FTSG implantation. Subsequent punch biopsies were then conducted on the 1st, 3rd, 7th, 14th, and 30th day to obtain samples for macroscopic (skin color), extracellular matrix (collagen), microscopic (PMN, macrophage, and fibroblast), and ELISA (TGFβ1 and PDGF) analysis to determine the level of wound healing activity. ImageJ software was used to photograph for macroscopic and extracellular matrix analysis. Results: Macroscopic, extracellular matrix, and ELISA evaluation show no significant difference in FTSG survival rates for all treatment groups. Microscopic examination showed an increase in PMN, macrophage, and fibroblast levels with PRFM application showing higher increases in all observed microscopic variables compared to PRP and control. Conclusion: This study observed that both PRFM and PRP as autologous platelet preparation accelerate wound healing in FTSG, with PRFM being superior due to the higher number of PMN, macrophage, and fibroblast. © 2021 The Authors
atropine; collagen; collagen type 1; hemoglobin; isoflurane; ketamine; lidocaine; platelet derived growth factor; platelet-rich fibrin; transforming growth factor beta1; xylazine; animal experiment; animal model; animal tissue; Article; enzyme linked immunosorbent assay; exosome; extracellular matrix; female; fibroblast; full thickness skin graft; glomerulus filtration rate; graft survival; histology; macrophage; male; microscopy; neutrophil; nonhuman; platelet-rich plasma cell; punch biopsy; scientific literature; skin injury; survival rate; thrombocyte; wound healing
Elsevier Ltd
20490801
Article
Q3
410
11547