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Publikasi Scopus 2024 per tanggal 30 November 2024 (994 artikel)
Publikasi Scopus 2024 per tanggal 30 November 2024 (994 artikel)
Hidayati, Wahyu (59397535600); Hidayati, Desti (59261136700); Khairindra, Fathur Luthfiano (59261865800); Umakappa, Munawir (59261136800); Sudarmono, Pratiwi Pudjilestari (6507855437); Farida, Siti (57193707159); Wirabrata, I Gede Made (59261312900); Dewi, Beti Ernawati (24076058600)
Prone-to-Infectivity of Omicron BA.2 Subvariant from Indonesia on ACE-2 Expressing Cell Lines
2024
Journal of Advanced Research in Micro and Nano Engineering
21
1
54
65
11
0
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85201266492&doi=10.37934%2farmne.21.1.5465&partnerID=40&md5=20169fc4fe64b02b79f23dbc6891e428
Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, Jakarta, 13460, Indonesia; Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, 10320, Indonesia; Infectious Disease and Immunology Cluster, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universi tas Indonesia, Jakarta, 10430, Indonesia; Department of Medical Pharmacy, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Agency for Health Policies Development, Indonesian Ministry of Health, Jakarta, 10560, Indonesia
SARS-CoV-2 virus undergoes mutation, leading to the virus's evolution and modifications in the characteristics of the virus. Omicron, including BA.2 subvariant, is currently the predominant variant in SARS-CoV-2 infection. There are no reports regarding its properties or the utilization of BA.2 Indonesian isolate for therapy and vaccine development. Therefore, this study evaluated appropriate host cells for Omicron BA.2 Indonesian isolate via susceptibility tests. The Omicron BA.2 from Indonesia was exposed to three mammalian-ACE2-expressing cell lines. Sharing amino acids between BA.2 from Indonesia and previous VOC Omicron subvariants was performed using a simple in silico comparison method. The results showed that the virus could not infect HepG2 and Huh-7D12 due to no foci forming on those cell lines. Moreover, we also found that BA.2 Indonesian isolate has a unique amino acid alteration on spike protein. According to the findings, the Omicron BA.2 from Indonesia could propagate on Vero E6 cell lines, and the mutations could play a role in the virus's changing infection mechanism. A deeper in vitro and in silico experiment could enhance the findings by comparing all BA.2 sequences from Indonesia and analyzing the infection mechanism by each single mutation using pseudovirus. © 2024, Penerbit Akademia Baru. All rights reserved.
Universitas Indonesia, UI, (396/PL.040/H.1/03/2021); Universitas Indonesia, UI
This study was supported by Universitas Indonesia [grant number: 396/PL.040/H.1/03/2021.K.].
Penerbit Akademia Baru
27568210
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