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Publikasi Scopus 2024 per tanggal 30 November 2024 (994 artikel)
Publikasi Scopus 2024 per tanggal 30 November 2024 (994 artikel)
Permatasari, Happy Kurnia (57194728338); Abshori, Nuril Farid (59144943300); Syahputra, Rony Abdi (57216124200); Harahap, Urip (55656293200); Amalia, Nurlinah (58315596900); Kumalawati, Dian Aruni (36721370800); Mayulu, Nelly (57303104200); Taslim, Nurpudji Astuti (57194571794); Tallei, Trina Ekawati (57193317686); Tjandrawinata, Raymond Rubianto (6603266135); Wiyarta, Elvan (57221521342); Pramono, Adriyan (56825779200); Kim, Bonglee (51663469700); Tsopmo, Apollinaire (6602260089); Serra-Majem,
Novel Functional Food Properties of Forest Onion (Eleutherine bulbosa Merr.) Phytochemicals for Treating Metabolic Syndrome: New Insights from a Combined Computational and In Vitro Approach
2024
Nutrients
16
10
1
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85194219741&doi=10.3390%2fnu16101441&partnerID=40&md5=263cd6110a09906cb804a5055e32ae8e
Department of Biochemistry and Biomolecular, Faculty of Medicine, University of Brawijaya, Malang, 65145, Indonesia; Medical Study Program, Faculty of Medicine, State Islamic University of Maulana Malik Ibrahim (UIN Maulana Malik Ibrahim), Malang, 65144, Indonesia; Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia; Master Program of Biomedical Science, Faculty of Medicine, Brawijaya University, Malang, 65151, Indonesia; Department of Biological Sciences, Faculty of Sciences and Technology, State Islamic University of Sunan Kalijaga (UIN Sunan Kalijaga), 55281, Yogyakarta, Indonesia; Department of Nutrition, Faculty of Health Science, Muhammadiyah Manado University, Manado 95249, Indonesia; Division of Clinical Nutrition, Department of Nutrition, Faculty of Medicine, Hasanuddin University, Makassar, 90245, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado, 95115, Indonesia; Department of Biotechnology, Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, 12930, Jakarta, Indonesia; Department of Neurology, Faculty of Medicine, Universitas Indonesia-Dr. Cipto Mangunkusumo National Hospital, 10430, Jakarta, Indonesia; Department of Nutrition, Faculty of Medicine, Diponegoro University, Semarang, 50275, Indonesia; Center of Nutrition Research (CENURE), Diponegoro University, Semarang, 50275, Indonesia; Department of Pathology, College of Korean Medicine, Kyung Hee University, 02447, Seoul, South Korea; Food Science Program, Department of Chemistry, Institute of Biochemistry, Carleton University, 1125 Colonel by Drive, Ottawa, ON K1S 5B6, Canada; Centro de Investigación Biomédica en Red Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), Institute of Health Carlos III, 28029, Madrid, Spain; Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, Las Palmas, 35001, Spain
Metabolic syndrome is a global health problem. The use of functional foods as dietary components has been increasing. One food of interest is forest onion extract (FOE). This study aimed to investigate the effect of FOE on lipid and glucose metabolism in silico and in vitro using the 3T3-L1 mouse cell line. This was a comprehensive study that used a multi-modal computational network pharmacology analysis and molecular docking in silico and 3T3-L1 mouse cells in vitro. The phytochemical components of FOE were analyzed using untargeted ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS). Next, an in silico analysis was performed to determine FOE's bioactive compounds, and a toxicity analysis, protein target identification, network pharmacology, and molecular docking were carried out. FOE's effect on pancreatic lipase, α-glucosidase, and α-amylase inhibition was determined. Finally, we determined its effect on lipid accumulation and MAPK8, PPARG, HMGCR, CPT-1, and GLP1 expression in the preadipocyte 3T3-L1 mouse cell line. We showed that the potential metabolites targeted glucose and lipid metabolism in silico and that FOE inhibited pancreatic lipase levels, α-glucosidase, and α-amylase in vitro. Furthermore, FOE significantly (p < 0.05) inhibits targeted protein expressions of MAPK8, PPARG, HMGCR, CPT-1, and GLP-1 in vitro in 3T3-L1 mouse cells in a dose-dependent manner. FOE contains several metabolites that reduce pancreatic lipase levels, α-glucosidase, α-amylase, and targeted proteins associated with lipid and glucose metabolism in vitro.
3T3-L1 Cells; alpha-Amylases; alpha-Glucosidases; Animals; Computer Simulation; Functional Food; Glucose; Lipase; Lipid Metabolism; Metabolic Syndrome; Mice; Molecular Docking Simulation; Network Pharmacology; Onions; Phytochemicals; Plant Extracts; PPAR gamma; Tandem Mass Spectrometry; alpha glucosidase; amylase; glucose; peroxisome proliferator activated receptor gamma; phytochemical; plant extract; triacylglycerol lipase; 3T3-L1 cell line; animal; chemistry; computer simulation; drug effect; drug therapy; functional food; lipid metabolism; metabolic syndrome X; metabolism; molecular docking; mouse; onion; systems pharmacology; tandem mass spectrometry
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