Publikasi Scopus FKUI 2021 per tanggal 31 Oktober 2021 (739 artikel)

Authors_(Last_Name)

Permata T.B.M., Sato H., Gu W., Kakoti S., Uchihara Y., Yoshimatsu Y., Sato I., Kato R., Yamauchi M., Suzuki K., Oike T., Tsushima Y., Gondhowiardjo S., Ohno T., Yasuhara T., Shibata A.

Author S Id

57197808751;55697961900;57211574572;57197814645;57221723636;57284438600;57285097100;57204087445;8307897300;55550919200;36453136000;57284002500;6508327402;35395665700;56562637100;8323572900;

Title

High linear energy transfer carbon-ion irradiation upregulates PD-L1 expression more significantly than X-rays in human osteosarcoma U2OS cells

Year

2021

Source Title

Journal of Radiation Research

Volume

Issue

Art No

Page Start

773

Page End

781

Page Count

Cited By

Link

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115918333&doi=10.1093%2fjrr%2frrab050&partnerID=40&md5=a1550b83230aa8ab90277d2716e9a556

Affiliations

Department of Radiation Oncology, Gunma University, Gunma, Maebashi, 371-8511, Japan; Department of Radiation Oncology, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo Hospital, Jakarta, 10430, Indonesia; Gunma University Heavy IonMedical Center, Gunma, Maebashi, 371-8511, Japan; Gunma University Initiative for Advanced Research (GIAR), Gunma University, Gunma, Maebashi, 371-8511, Japan; Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School OfMedicine, Gunma, Maebashi, 371-8511, Japan; Laboratory of Molecular Radiology, Center for Disease Biology and Integrative Medicine, Graduate School OfMedicine, The University OfTokyo, Bunkyo-ku, Tokyo, 113-8655, Japan; Department of Radiation Biology and Protection, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, 852-8523, Japan; Department of Radiation Medical Science, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, 852-8523, Japan

Authors With Affiliations

Permata, T.B.M., Department of Radiation Oncology, Gunma University, Gunma, Maebashi, 371-8511, Japan, Department of Radiation Oncology, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo Hospital, Jakarta, 10430, Indonesia; Sato, H., Department of Radiation Oncology, Gunma University, Gunma, Maebashi, 371-8511, Japan, Gunma University Heavy IonMedical Center, Gunma, Maebashi, 371-8511, Japan; Gu, W., Gunma University Initiative for Advanced Research (GIAR), Gunma University, Gunma, Maebashi, 371-8511, Japan, Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School OfMedicine, Gunma, Maebashi, 371-8511, Japan; Kakoti, S., Department of Radiation Oncology, Gunma University, Gunma, Maebashi, 371-8511, Japan, Gunma University Initiative for Advanced Research (GIAR), Gunma University, Gunma, Maebashi, 371-8511, Japan; Uchihara, Y., Gunma University Initiative for Advanced Research (GIAR), Gunma University, Gunma, Maebashi, 371-8511, Japan; Yoshimatsu, Y., Gunma University Initiative for Advanced Research (GIAR), Gunma University, Gunma, Maebashi, 371-8511, Japan, Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School OfMedicine, Gunma, Maebashi, 371-8511, Japan; Sato, I., Gunma University Initiative for Advanced Research (GIAR), Gunma University, Gunma, Maebashi, 371-8511, Japan; Kato, R., Laboratory of Molecular Radiology, Center for Disease Biology and Integrative Medicine, Graduate School OfMedicine, The University OfTokyo, Bunkyo-ku, Tokyo, 113-8655, Japan; Yamauchi, M., Department of Radiation Biology and Protection, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, 852-8523, Japan; Suzuki, K., Department of Radiation Medical Science, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, 852-8523, Japan; Oike, T., Department of Radiation Oncology, Gunma University, Gunma, Maebashi, 371-8511, Japan; Tsushima, Y., Gunma University Initiative for Advanced Research (GIAR), Gunma University, Gunma, Maebashi, 371-8511, Japan; Gondhowiardjo, S., Department of Radiation Oncology, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo Hospital, Jakarta, 10430, Indonesia; Ohno, T., Department of Radiation Oncology, Gunma University, Gunma, Maebashi, 371-8511, Japan, Gunma University Heavy IonMedical Center, Gunma, Maebashi, 371-8511, Japan; Yasuhara, T., Laboratory of Molecular Radiology, Center for Disease Biology and Integrative Medicine, Graduate School OfMedicine, The University OfTokyo, Bunkyo-ku, Tokyo, 113-8655, Japan; Shibata, A., Gunma University Initiative for Advanced Research (GIAR), Gunma University, Gunma, Maebashi, 371-8511, Japan

Abstract

Programmed death ligand 1 (PD-L1) expression on the surface of cancer cells affects the efficacy of anti-PD-1/PD-L1 immune checkpoint therapy. However, the mechanism underlying PD-L1 expression in cancer cells is not fully understood, particularly after ionizing radiation (IR). Here, we examined the impact of high linear energy transfer (LET) carbon-ion irradiation on the expression of PD-L1 in human osteosarcoma U2OS cells. We found that the upregulation of PD-L1 expression after high LET carbon-ion irradiation was greater than that induced by X-rays at the same physical and relative biological effectiveness (RBE) dose, and that the upregulation of PD-L1 induced by high LET carbon-ion irradiation was predominantly dependent on ataxia telangiectasia and Rad3-related (ATR) kinase activity. Moreover, we showed that the downstream signaling, e.g. STAT1 phosphorylation and IRF1 expression, was upregulated to a greater extent after high LET carbon-ion irradiation than X-rays, and that IRF1 upregulation was also ATR dependent. Finally, to visualize PD-L1 molecules on the cell surface in 3D, we applied immunofluorescence-based super-resolution imaging. The three-dimensional structured illumination microscopy (3D-SIM) analyses revealed substantial increases in the number of presented PD-L1 molecules on the cell surface after high LET carbon-ion irradiation compared with X-ray irradiation. ? 2021 The Author(s) 2021. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology.

Author Keywords

anti-PD-1/PD-L1 therapy; DNA damage response; high linear energy transfer (LET) carbon-ion therapy; PD-L1 expression

Index Keywords

Publisher

Oxford University Press

Issn

4493060

Isbn

Pubmed Id

34196706

Document Type

Article

Sjr Best Quartile

Sjr

643

Sjr Rank

7838