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Publikasi Scopus Covid-19 Per 14 Agustus 2024 (559 artikel)
Publikasi Scopus Covid-19 Per 14 Agustus 2024 (559 artikel)
Adhiyanto, Chris (6507604294); Suwarsono, Erike A. (57218531962); Hendarmin, Laifa A. (8560333500); Harriyati, Zeti (57224479053); Sari, Flori R. (36455410900); Ekayanti, Fika (56303935200); Wulandari, Endah (55573167700); Kunarisasi, Siti (58000515900); Al Farisi, Mahfuzh A. (57998989700); Nareshwari, Devi R. (57999868000); Suryani (57224476625); Rochmawati, Din F. (58000298900); Latifah, Ayu (58000299000); Freisleben, H.-J. (7003437337)
Detection of the SARS-CoV-2 Omicron Variant in COVID-19 Patients from South Tangerang Using SNP-Probes S371L and K417N
2022
Journal of Pure and Applied Microbiology
16
4
2712
2723
11
0
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85143655349&doi=10.22207%2fJPAM.16.4.42&partnerID=40&md5=80139a572885bc3132e9761e6f0fce18
Medical Research Laboratory, Faculty of Medicine, Syarif Hidayatullah State Islamic University, Jakarta, Indonesia; Faculty of Medicine, Syarif Hidayatullah State Islamic University, Jakarta, Indonesia; Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Regional Public COVID-19 Vaccination Centre, Eltville am Rhein, Germany
The COVID-19 pandemic caused by the SARS-CoV-2 virus has posed a global challenge. Experts from various branches of science have endeavoured to find solutions to control its spread, one of which has been the quick and precise detection of the virus and its variants in patients. This study aimed to detect the presence of SARS-CoV-2, notably the rapidly spreading Omicron variant, using the spike (S)-gene target failure (SGTF) and S-gene target positive (SGTP) with the principle of the single nucleotide polymorphism (SNP)-probe test. Our descriptive experimental approach detected Omicron variants with the SNP-probe technique using samples of SARS-CoV-2 patients and controls. The probes were designed to recognize the nucleotide code of the amino acids in positions 371 and 417 of SARS-CoV-2. The existence of variants was monitored by the presence or absence of a fluorescence signal, which was translated into a sigmoidal graph using a real-time (RT)-PCR machine. One hundred and twelve samples that had tested positive for SARS-CoV-2 and the Omicron variant using a registered commercial kit showed a similar result to our in-house-developed SNP-probe 371 and 417 assays. The results of this study indicate that the SNP-probe we designed can be used in the detection of the SARS-CoV-2 Omicron variant. © The Author(s) 2022.
amino acid; coronavirus spike glycoprotein; nucleotide; Article; controlled study; coronavirus disease 2019; fluorescence; gene targeting; genetic association; human; Indonesia; major clinical study; nonhuman; nucleotide sequence; real time polymerase chain reaction; SARS-CoV-2 Alpha; SARS-CoV-2 Beta; SARS-CoV-2 Delta; SARS-CoV-2 Omicron; screening; single nucleotide polymorphism; virus strain; whole genome sequencing
Faculty of Medicine Syarif Hidayatullah State Islamic University Jakarta; Ministry of Health of the Republic of Indonesia
The authors would like to thank the members of the COVID-19 laboratory Faculty of Medicine Syarif Hidayatullah State Islamic University Jakarta, Ministry of Health Republic Indonesia, PT. Biopharma and PT. Elokarsa Utama for their support in conducting the research.
Journal of Pure and Applied Microbiology
09737510
Article
Q4