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Publikasi Scopus 2023 per tanggal 31 Januari 2023 (41 artikel)
Publikasi Scopus 2023 per tanggal 31 Januari 2023 (41 artikel)
Down-Regulation of miR-93 Negatively Correlates with Overexpression of VEGFA and MMP3 in Endometriosis: A Cross-Sectional Study
2023
International Journal of Fertility and Sterility
17
1
28
33
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145563362&doi=10.22074%2fIJFS.2022.543884.1233&partnerID=40&md5=00111fdeecd07bcb6dca6d8141203c88
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Human Reproduction, Infertility, and Family Planning Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Yasmin IVF Clinic, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia
Background: Endometriosis is identified as presence of the endometrium outside the uterine cavity. Retrograde men-struation contributes to the endometrial tissue implantation and the establishment of endometriotic lesions at ectopic sites. It has been suggested that the endometriotic lesions are rich in angiogenic growth factors, while they have an essential role in survival and invasion of these cells. We investigated regulation of microRNA-93 (miR-93) and its involvement with vascular endothelial growth factor A (VEGFA) and matrix metalloproteinase (MMP) 3 expression in women with endometriosis. Materials and Methods: This was a cross-sectional study at Central Surgical Installation, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, between October 2020 and November 2021. Eutopic and ectopic endometrial tissues were collected from 30 subjects with laparoscopically-confirmed endometriotic women. Normal endometrial cells of non-endometriosis women served as controls. Total RNA was isolated from all samples and a quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was used to analyze the expression of miR-93, VEGFA and MMP3. Results: There was no significant difference in the expression levels of VEGFA (2.14 ± 0.50, P=0.719) and MMP3 (2.99 ± 0.42, P=0.583) between endometriotic lesions of endometriosis women and the healthy endometrium. Expression of miR-93 was significantly lower in the eutopic endometrium (16.7 fold) and ectopic endometriotic lesion (20 fold) compared to the normal endometrium (P<0.001). Furthermore, we also observed a significant correlation between miR-93 and VEGFA expression in eutopic endometrium obtained from women with endometriosis (r=-0.544, P=0.029). Expression of the miR-93 was also negatively correlated with MMP3 expression in both eutopic (r=-0.412, P=0.01) and ectopic (r=-0.539, P=0.03) endometrial cells of women with endometriosis. Conclusion: VEGFA and MMP3 expression levels trended to be increased in both eutopic and ectopic endometrial tissues of endometriosis women, while down-regulation of miR-93 might be involved in the alteration of VEGFA and MMP3 in endometriosis. © 2023, Royan Institute (ACECR). All rights reserved.
microRNA; microRNA 93; stromelysin; unclassified drug; vasculotropin A; adult; angiogenesis; Article; clinical article; controlled study; cross-sectional study; down regulation; echography; ectopic tissue; endometriosis; endometrium; endometrium cell; eutopic endometrium; female; human; human tissue; in vitro fertilization; laparoscopy; menstrual cycle; mRNA expression level; real time reverse transcription polymerase chain reaction; reverse transcription polymerase chain reaction; RNA extraction; scoring system; upregulation
NKB-1538/UN2.RST/HKP.05.00/2020; Universitas Indonesia, UI
The authors would like to thank Universitas Indonesia for funding this research through PUTI grant with contract NKB-1538/UN2.RST/HKP.05.00/2020.
The authors would like to thank Universitas Indonesia for funding this research through PUTI grant with contract
Royan Institute (ACECR)
2008076X
Article
Q2
465
10672
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