Riset dan Pengabdian Masyarakat
Do Research Sosial Service and Innovation
Publikasi Scopus 2024 per tanggal 11 Februari 2025 (1119 artikel)
Publikasi Scopus 2024 per tanggal 11 Februari 2025 (1119 artikel)
Pustimbara, Anantya (57217086984); Umami, Rahma Wirdatul (59448974900); Prakoso, Nurul Muhammad (59423442700); Rozaliyani, Anna (57203065912); Zaini, Jamal (57221833355); Dwiranti, Astari (55151437900); Ogura, Shun-Ichiro (57592233200); Bowolaksono, Anom (57205093224)
Hemin Promotes Higher Effectiveness of Aminolevulinic-Photodynamic Therapy (ALA-PDT) in A549 Lung Cancer Cell Line by Interrupting ABCG2 Expression
2024
Medical sciences (Basel, Switzerland)
12
4
0
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85210549480&doi=10.3390%2fmedsci12040066&partnerID=40&md5=fdb6c4d0c1fa7958d27fe12433a8e570
Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok Campus, Depok, 16424, Indonesia; School of Life Science and Technology, Tokyo Institute of Technology, Midori-ku, Nagatsuta-cho, Yokohama, 226-8501, Japan; Department of Parasitology, Faculty of Medicine, Universitas Indonesia, 10430, Jakarta, Indonesia; Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia, Persahabatan National Respiratory Referral Hospital, 13230, Jakarta, Indonesia
BACKGROUND/OBJECTIVES: Due to concerns about drug resistance and side effects, the discovery of improved drugs for lung cancer has attracted studies to find an effective and safe treatment. Aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) is a cancer treatment with minimal side effects. However, ALA-PDT effectiveness can be hindered by ABCG2 and ABCB1 transporters impeding PpIX accumulation. Combining ALA with other substances can enhance PpIX accumulation. Hemin is a potential substance due to its antitumor properties and may be involved in regulating the ABCG2 and ABCB1 expressions. METHODS: The objective of this report is to analyze the effects of administering a combination of hemin and ALA after 48 h on A549 lung cancer cells by quantifying cell viability, intracellular PpIX, and ROS accumulation, completed by ABCG2 and ABCB1 expressions. RESULTS: Our data indicate that the combination of hemin and ALA followed by photoirradiation decreased the viability of A549 cells, which was due to increased intracellular PpIX and ROS. The expression of ABCG2 mRNA was significantly decreased after ALA-hemin treatment, while the ABCB1 mRNA expression increased. This result might suggest that ABCG2 plays a greater role than ABCB1 in regulating the PpIX accumulation in A549 lung cancer cells. CONCLUSIONS: The combination of ALA and hemin followed by photoirradiation offers a promising novel treatment for lung cancer, and further evaluations of this therapy are required.
A549 Cells; Aminolevulinic Acid; ATP Binding Cassette Transporter, Subfamily B; ATP Binding Cassette Transporter, Subfamily G, Member 2; ATP-Binding Cassette Transporters; Cell Survival; Gene Expression Regulation, Neoplastic; Hemin; Humans; Lung Neoplasms; Neoplasm Proteins; Photochemotherapy; Photosensitizing Agents; Protoporphyrins; Reactive Oxygen Species; ABC transporter; ABC transporter subfamily B; ABCB1 protein, human; ABCG2 protein, human; aminolevulinic acid; breast cancer resistance protein; hemin; photosensitizing agent; protoporphyrin; reactive oxygen metabolite; tumor protein; A-549 cell line; cell survival; drug effect; drug therapy; gene expression regulation; human; lung tumor; metabolism; photochemotherapy
20763271
39584916
Article
Q1
1273
2973