Publikasi Scopus 2024 per tanggal 29 Februari 2024 (130 artikel)

Khayrani A.C.; Fahmi M.; Nurhayati R.W.; Manas N.H.A.; Suhaeri M.
Khayrani, Apriliana Cahya (54885351000); Fahmi, Muhammad (58891461500); Nurhayati, Retno Wahyu (55748436600); Manas, Nor Hasmaliana Abdul (56364749500); Suhaeri, Muhammad (56183087500)
54885351000; 58891461500; 55748436600; 56364749500; 56183087500
Effect of Freeze-Thaw Cycles Method to Transfersome Characteristics for Growth Protein Encapsulation
2024
International Journal of Technology
15
2
267
278
11
Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, West Java, Depok, 16464, Indonesia; Research Center for Biomedical Engineering, Faculty of Engineering, Universitas Indonesia, West Java, Depok, 16464, Indonesia; Stem Cells and Tissue Engineering Research Cluster, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Central Jakarta, 10430, Indonesia; Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, Johor, 81310, Malaysia; Institute of Bioproduct Development, Universiti Teknologi Malaysia, Johor, 81310, Malaysia; Universitas Indonesia Hospital, Universitas Indonesia, West Java, Depok, 16424, Indonesia
Khayrani A.C., Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, West Java, Depok, 16464, Indonesia, Research Center for Biomedical Engineering, Faculty of Engineering, Universitas Indonesia, West Java, Depok, 16464, Indonesia; Fahmi M., Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, West Java, Depok, 16464, Indonesia; Nurhayati R.W., Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, West Java, Depok, 16464, Indonesia, Research Center for Biomedical Engineering, Faculty of Engineering, Universitas Indonesia, West Java, Depok, 16464, Indonesia, Stem Cells and Tissue Engineering Research Cluster, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Central Jakarta, 10430, Indonesia; Manas N.H.A., Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, Johor, 81310, Malaysia, Institute of Bioproduct Development, Universiti Teknologi Malaysia, Johor, 81310, Malaysia; Suhaeri M., Universitas Indonesia Hospital, Universitas Indonesia, West Java, Depok, 16424, Indonesia
Transfersome, a lipid-based nanovesicle, can be a suitable tool to improve the delivery of valuable growth factors. Through transfersome technology, growth factors and active compounds can be transferred transdermally without the need for invasive delivery procedures. In this study, we evaluated the impact of freeze-thaw cycles on transfersome characteristics, particularly particle size, polydispersity, and encapsulation efficiency. Transfersome particles were prepared from dipalmitoylphosphatidylcholine (DPPC) and Tween 80 with a 97.5:2.5 w/w% using thin film hydration at a temperature of 45° - 50°C. Then, the transfersome suspension was subjected to repeated freeze-thaw for 1 minute of freezing and 3 minutes of thawing. The protein release from all transfersome samples were evaluated using Bradford assay, while the particle size and polydispersity were determined with a dynamic scattering analyzer. It was found that freeze-thaw increased encapsulation efficiency, particle size, and polydispersity of transfersomes up to 81.63±0.00%, 180.70±0.87 nm, and 0.369±0.02, respectively, from those without freeze-thaw steps (73.35±0.03%, 144.93±0.21 nm and 0.202±0.02). Moreover, freeze-thawed transfersomes exhibited a release of up to 52.80% of loaded protein within 78 hours, in contrast to 37.48% in non-freeze-thawed transfersomes. This study shows that an additional freeze-thaw step is a promising method to improve the properties of transfersome particles, especially encapsulation efficiency and sustained protein release. © 2024, Faculty of Engineering. All rights reserved.
Dipalmitoylphosphatidylcholine; Encapsulation; Freeze-thaw; Growth protein; Transfersome
Korea Institute of Science and Technology, KIST, (PKS-193/UN2.INV/HKP.05/2021); Direktorat Riset and Pengembangan, Universitas Indonesia, DRPM UI, (NKB-800/UN2.RST/HKP.05.00/2023); Kementerian Pendidikan, Kebudayaan, Riset, dan Teknologi, MECRT
This research was partially funded by Korea Institute of Science and Technology (KIST) School Partnership Project 2022 awarded to Dr. Muhammad Suhaeri and Indonesian National Research Priority Research Funding 2021 (Program Flagship Pendanaan Prioritas Riset Nasional (PRN) untuk Perguruan Tinggi 2021; No. PKS-193/UN2.INV/HKP.05/2021) from Indonesian Ministry of Education, Culture, Research, and Te
Faculty of Engineering, Universitas Indonesia
20869614
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